Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-03

AUTHORS

Frank J Hernandez, Lingyan Huang, Michael E Olson, Kristy M Powers, Luiza I Hernandez, David K Meyerholz, Daniel R Thedens, Mark A Behlke, Alexander R Horswill, James O McNamara

ABSTRACT

Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the activity of the S. aureus secreted nuclease, micrococcal nuclease (MN). Several short synthetic oligonucleotides, rendered resistant to mammalian serum nucleases by various chemical modifications and flanked with a fluorophore and quencher, were activated upon degradation by purified MN and in S. aureus culture supernatants. A probe consisting of a pair of deoxythymidines flanked by several 2'-O-methyl-modified nucleotides was activated in culture supernatants of S. aureus but not in culture supernatants of several other pathogenic bacteria. Systemic administration of this probe to mice bearing S. aureus muscle infections resulted in probe activation at the infection sites in an MN-dependent manner. This new bacterial imaging approach has potential clinical applicability for infections with S. aureus and several other medically important pathogens. More... »

PAGES

301-306

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nm.3460

DOI

http://dx.doi.org/10.1038/nm.3460

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029423318

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24487433


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