Regulation of Lck activity by CD4 and CD28 in the immunological synapse View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-02-04

AUTHORS

Amy D. Holdorf, Kyeong-Hee Lee, W. Richard Burack, Paul M. Allen, Andrey S. Shaw

ABSTRACT

Although the Src family tyrosine kinase Lck is essential for T cell receptor (TCR) signaling, whether or how Lck is activated is unknown. Using a phosphospecific antiserum to Lck, we show here that Lck becomes autophosphorylated when T cells are stimulated by antigen-presenting cells (APCs). We found that TCR cross-linking alone could not stimulate Lck autophosphorylation and CD45 was not required for this process. Instead, the T cell accessory molecules CD4 and CD28 cooperated to induce autophosphorylation of Lck. CD4 recruited Lck to the T cell–APC interface, whereas CD28 sustained Lck activation. These data show how the multiple interactions afforded by the immunological synapse drive efficient and highly specific signaling. More... »

PAGES

259-264

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ni761

DOI

http://dx.doi.org/10.1038/ni761

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002992988

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11828322


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD28 Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD4 Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphocyte Specific Protein Tyrosine Kinase p56(lck)", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Antigen, T-Cell", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Synapses", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Holdorf", 
        "givenName": "Amy D.", 
        "id": "sg:person.012762313111.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012762313111.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Kyeong-Hee", 
        "id": "sg:person.01110535621.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110535621.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Burack", 
        "givenName": "W. Richard", 
        "id": "sg:person.0722532117.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722532117.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Allen", 
        "givenName": "Paul M.", 
        "id": "sg:person.014057324042.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014057324042.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.4367.6", 
          "name": [
            "Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shaw", 
        "givenName": "Andrey S.", 
        "id": "sg:person.01204351550.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204351550.49"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-1-4684-5943-2_10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025827964", 
          "https://doi.org/10.1007/978-1-4684-5943-2_10"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/25764", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001596774", 
          "https://doi.org/10.1038/25764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048719919", 
          "https://doi.org/10.1038/ni737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/385650a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015845219", 
          "https://doi.org/10.1038/385650a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-02-04", 
    "datePublishedReg": "2002-02-04", 
    "description": "Although the Src family tyrosine kinase Lck is essential for T cell receptor (TCR) signaling, whether or how Lck is activated is unknown. Using a phosphospecific antiserum to Lck, we show here that Lck becomes autophosphorylated when T cells are stimulated by antigen-presenting cells (APCs). We found that TCR cross-linking alone could not stimulate Lck autophosphorylation and CD45 was not required for this process. Instead, the T cell accessory molecules CD4 and CD28 cooperated to induce autophosphorylation of Lck. CD4 recruited Lck to the T cell\u2013APC interface, whereas CD28 sustained Lck activation. These data show how the multiple interactions afforded by the immunological synapse drive efficient and highly specific signaling.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/ni761", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1022778", 
        "issn": [
          "1529-2908", 
          "1529-2916"
        ], 
        "name": "Nature Immunology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "3"
      }
    ], 
    "keywords": [
      "antigen-presenting cells", 
      "Src family tyrosine kinase Lck", 
      "immunological synapse", 
      "autophosphorylation of Lck", 
      "tyrosine kinase Lck", 
      "accessory molecules CD4", 
      "cell receptor signaling", 
      "cell-APC interface", 
      "Lck autophosphorylation", 
      "phosphospecific antiserum", 
      "CD4", 
      "kinase Lck", 
      "receptor signaling", 
      "CD28", 
      "Lck activity", 
      "specific signaling", 
      "Lck activation", 
      "Lck", 
      "autophosphorylation", 
      "synapse", 
      "signaling", 
      "multiple interactions", 
      "cells", 
      "CD45", 
      "TCR", 
      "antiserum", 
      "activation", 
      "regulation", 
      "activity", 
      "interaction", 
      "data", 
      "process", 
      "interface", 
      "family tyrosine kinase Lck", 
      "cell accessory molecules CD4", 
      "molecules CD4"
    ], 
    "name": "Regulation of Lck activity by CD4 and CD28 in the immunological synapse", 
    "pagination": "259-264", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002992988"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ni761"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11828322"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ni761", 
      "https://app.dimensions.ai/details/publication/pub.1002992988"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_361.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/ni761"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ni761'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ni761'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ni761'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ni761'


 

This table displays all metadata directly associated to this object as RDF triples.

182 TRIPLES      22 PREDICATES      76 URIs      64 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ni761 schema:about N2ed8713cd91840c2af5c712cb4fb4013
2 N41ac311d1fe6454cac29358d767eda47
3 N44acdc456d0346eabcfbe87bc1af91c3
4 N8582e5ce32b049dcb953d6d4c4db48bc
5 N9498e2af08b0478cb97199b730dc2a6b
6 N9ffe27817de54d23818012913d833be5
7 Nb18b35854e0148daa9dd1595ae6d313c
8 Nbfac29e9886649d984615ba6af43991f
9 Ncdd3b62d425847e3b1d878d89655e9c5
10 Nd0cd3419da4343c78d9165af5e1db4fb
11 anzsrc-for:11
12 anzsrc-for:1107
13 schema:author N87c730055d8b449993ce9625cfd659e4
14 schema:citation sg:pub.10.1007/978-1-4684-5943-2_10
15 sg:pub.10.1038/25764
16 sg:pub.10.1038/385650a0
17 sg:pub.10.1038/ni737
18 schema:datePublished 2002-02-04
19 schema:datePublishedReg 2002-02-04
20 schema:description Although the Src family tyrosine kinase Lck is essential for T cell receptor (TCR) signaling, whether or how Lck is activated is unknown. Using a phosphospecific antiserum to Lck, we show here that Lck becomes autophosphorylated when T cells are stimulated by antigen-presenting cells (APCs). We found that TCR cross-linking alone could not stimulate Lck autophosphorylation and CD45 was not required for this process. Instead, the T cell accessory molecules CD4 and CD28 cooperated to induce autophosphorylation of Lck. CD4 recruited Lck to the T cell–APC interface, whereas CD28 sustained Lck activation. These data show how the multiple interactions afforded by the immunological synapse drive efficient and highly specific signaling.
21 schema:genre article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N06e897dd0b9b4b07aef148007254a275
25 N75cba6ea60964cf2a44e17af00a9040a
26 sg:journal.1022778
27 schema:keywords CD28
28 CD4
29 CD45
30 Lck
31 Lck activation
32 Lck activity
33 Lck autophosphorylation
34 Src family tyrosine kinase Lck
35 TCR
36 accessory molecules CD4
37 activation
38 activity
39 antigen-presenting cells
40 antiserum
41 autophosphorylation
42 autophosphorylation of Lck
43 cell accessory molecules CD4
44 cell receptor signaling
45 cell-APC interface
46 cells
47 data
48 family tyrosine kinase Lck
49 immunological synapse
50 interaction
51 interface
52 kinase Lck
53 molecules CD4
54 multiple interactions
55 phosphospecific antiserum
56 process
57 receptor signaling
58 regulation
59 signaling
60 specific signaling
61 synapse
62 tyrosine kinase Lck
63 schema:name Regulation of Lck activity by CD4 and CD28 in the immunological synapse
64 schema:pagination 259-264
65 schema:productId N52d4b42f4bca4fd99649cf7422eaf535
66 N99a07f60b3ae4b6886642a09bc61cda1
67 Nad1d780e0f614ad4b0c7a529e822f4f2
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002992988
69 https://doi.org/10.1038/ni761
70 schema:sdDatePublished 2022-01-01T18:12
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher Nf3236570f9e849f18f22a11508ef7709
73 schema:url https://doi.org/10.1038/ni761
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N06e897dd0b9b4b07aef148007254a275 schema:volumeNumber 3
78 rdf:type schema:PublicationVolume
79 N2ed8713cd91840c2af5c712cb4fb4013 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
81 rdf:type schema:DefinedTerm
82 N3997c1b1803548578d74012042fb02c4 rdf:first sg:person.01204351550.49
83 rdf:rest rdf:nil
84 N41ac311d1fe6454cac29358d767eda47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Animals
86 rdf:type schema:DefinedTerm
87 N44acdc456d0346eabcfbe87bc1af91c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Mice, Transgenic
89 rdf:type schema:DefinedTerm
90 N52d4b42f4bca4fd99649cf7422eaf535 schema:name dimensions_id
91 schema:value pub.1002992988
92 rdf:type schema:PropertyValue
93 N666a8a051f0449efab3684d823339904 rdf:first sg:person.01110535621.64
94 rdf:rest Na6432deada4d45f5b4eff88bb532ab95
95 N75cba6ea60964cf2a44e17af00a9040a schema:issueNumber 3
96 rdf:type schema:PublicationIssue
97 N8582e5ce32b049dcb953d6d4c4db48bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Receptors, Antigen, T-Cell
99 rdf:type schema:DefinedTerm
100 N87c730055d8b449993ce9625cfd659e4 rdf:first sg:person.012762313111.33
101 rdf:rest N666a8a051f0449efab3684d823339904
102 N939b30efe68a4b83955f1d4e57dc229d rdf:first sg:person.014057324042.67
103 rdf:rest N3997c1b1803548578d74012042fb02c4
104 N9498e2af08b0478cb97199b730dc2a6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Synapses
106 rdf:type schema:DefinedTerm
107 N99a07f60b3ae4b6886642a09bc61cda1 schema:name doi
108 schema:value 10.1038/ni761
109 rdf:type schema:PropertyValue
110 N9ffe27817de54d23818012913d833be5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Rabbits
112 rdf:type schema:DefinedTerm
113 Na6432deada4d45f5b4eff88bb532ab95 rdf:first sg:person.0722532117.72
114 rdf:rest N939b30efe68a4b83955f1d4e57dc229d
115 Nad1d780e0f614ad4b0c7a529e822f4f2 schema:name pubmed_id
116 schema:value 11828322
117 rdf:type schema:PropertyValue
118 Nb18b35854e0148daa9dd1595ae6d313c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name CD28 Antigens
120 rdf:type schema:DefinedTerm
121 Nbfac29e9886649d984615ba6af43991f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name CD4 Antigens
123 rdf:type schema:DefinedTerm
124 Ncdd3b62d425847e3b1d878d89655e9c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Mice
126 rdf:type schema:DefinedTerm
127 Nd0cd3419da4343c78d9165af5e1db4fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Phosphorylation
129 rdf:type schema:DefinedTerm
130 Nf3236570f9e849f18f22a11508ef7709 schema:name Springer Nature - SN SciGraph project
131 rdf:type schema:Organization
132 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
133 schema:name Medical and Health Sciences
134 rdf:type schema:DefinedTerm
135 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
136 schema:name Immunology
137 rdf:type schema:DefinedTerm
138 sg:journal.1022778 schema:issn 1529-2908
139 1529-2916
140 schema:name Nature Immunology
141 schema:publisher Springer Nature
142 rdf:type schema:Periodical
143 sg:person.01110535621.64 schema:affiliation grid-institutes:grid.4367.6
144 schema:familyName Lee
145 schema:givenName Kyeong-Hee
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110535621.64
147 rdf:type schema:Person
148 sg:person.01204351550.49 schema:affiliation grid-institutes:grid.4367.6
149 schema:familyName Shaw
150 schema:givenName Andrey S.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204351550.49
152 rdf:type schema:Person
153 sg:person.012762313111.33 schema:affiliation grid-institutes:grid.4367.6
154 schema:familyName Holdorf
155 schema:givenName Amy D.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012762313111.33
157 rdf:type schema:Person
158 sg:person.014057324042.67 schema:affiliation grid-institutes:grid.4367.6
159 schema:familyName Allen
160 schema:givenName Paul M.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014057324042.67
162 rdf:type schema:Person
163 sg:person.0722532117.72 schema:affiliation grid-institutes:grid.4367.6
164 schema:familyName Burack
165 schema:givenName W. Richard
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722532117.72
167 rdf:type schema:Person
168 sg:pub.10.1007/978-1-4684-5943-2_10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025827964
169 https://doi.org/10.1007/978-1-4684-5943-2_10
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/25764 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001596774
172 https://doi.org/10.1038/25764
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/385650a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015845219
175 https://doi.org/10.1038/385650a0
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/ni737 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048719919
178 https://doi.org/10.1038/ni737
179 rdf:type schema:CreativeWork
180 grid-institutes:grid.4367.6 schema:alternateName Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA
181 schema:name Department of Pathology and Immunology, Washington University School of Medicine, 660 S. Euclid, Campus Box 8118, 63110, St. Louis, MO, USA
182 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...