Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-12

AUTHORS

Christopher D Herring, Anu Raghunathan, Christiane Honisch, Trina Patel, M Kenyon Applebee, Andrew R Joyce, Thomas J Albert, Frederick R Blattner, Dirk van den Boom, Charles R Cantor, Bernhard Ø Palsson

ABSTRACT

We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts. More... »

PAGES

1406-1412

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng1906

DOI

http://dx.doi.org/10.1038/ng1906

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004277286

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17086184


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng1906'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng1906'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng1906'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng1906'


 

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