Genomic alterations in cultured human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-10

AUTHORS

Anirban Maitra, Dan E Arking, Narayan Shivapurkar, Morna Ikeda, Victor Stastny, Keyaunoosh Kassauei, Guoping Sui, David J Cutler, Ying Liu, Sandii N Brimble, Karin Noaksson, Johan Hyllner, Thomas C Schulz, Xianmin Zeng, William J Freed, Jeremy Crook, Suman Abraham, Alan Colman, Peter Sartipy, Sei-Ichi Matsui, Melissa Carpenter, Adi F Gazdar, Mahendra Rao, Aravinda Chakravarti

ABSTRACT

Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. Nevertheless, these dividing cells, like other cells, probably undergo spontaneous mutation at a rate of 10(-9) per nucleotide. Because each mutant has only a few progeny, the overall biological properties of the cell culture are not altered unless a mutation provides a survival or growth advantage. Clonal evolution that leads to emergence of a dominant mutant genotype may potentially affect cellular phenotype as well. We assessed the genomic fidelity of paired early- and late-passage hESC lines in the course of tissue culture. Relative to early-passage lines, eight of nine late-passage hESC lines had one or more genomic alterations commonly observed in human cancers, including aberrations in copy number (45%), mitochondrial DNA sequence (22%) and gene promoter methylation (90%), although the latter was essentially restricted to 2 of 14 promoters examined. The observation that hESC lines maintained in vitro develop genetic and epigenetic alterations implies that periodic monitoring of these lines will be required before they are used in in vivo applications and that some late-passage hESC lines may be unusable for therapeutic purposes. More... »

PAGES

1099-1103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng1631

DOI

http://dx.doi.org/10.1038/ng1631

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013244477

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16142235


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Mitochondrial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genome, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Johns Hopkins University", 
          "id": "https://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "McKusick-Nathans Institute of Genetic Medicine, Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maitra", 
        "givenName": "Anirban", 
        "id": "sg:person.01246576774.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246576774.91"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Arking", 
        "givenName": "Dan E", 
        "id": "sg:person.01304013052.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304013052.15"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Shivapurkar", 
        "givenName": "Narayan", 
        "id": "sg:person.0756533042.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756533042.24"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Ikeda", 
        "givenName": "Morna", 
        "id": "sg:person.01304607577.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304607577.06"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Stastny", 
        "givenName": "Victor", 
        "id": "sg:person.0745313276.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745313276.53"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kassauei", 
        "givenName": "Keyaunoosh", 
        "id": "sg:person.01104242053.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104242053.89"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Sui", 
        "givenName": "Guoping", 
        "id": "sg:person.01106342317.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106342317.32"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Cutler", 
        "givenName": "David J", 
        "id": "sg:person.015711065457.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015711065457.76"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Liu", 
        "givenName": "Ying", 
        "type": "Person"
      }, 
      {
        "familyName": "Brimble", 
        "givenName": "Sandii N", 
        "id": "sg:person.01026107356.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026107356.71"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Noaksson", 
        "givenName": "Karin", 
        "id": "sg:person.01205240623.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205240623.21"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Hyllner", 
        "givenName": "Johan", 
        "id": "sg:person.0613004244.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613004244.83"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Schulz", 
        "givenName": "Thomas C", 
        "id": "sg:person.01244530113.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244530113.11"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Zeng", 
        "givenName": "Xianmin", 
        "id": "sg:person.0754175020.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754175020.89"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Freed", 
        "givenName": "William J", 
        "id": "sg:person.01156511547.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156511547.21"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Crook", 
        "givenName": "Jeremy", 
        "id": "sg:person.01057672623.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057672623.94"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Abraham", 
        "givenName": "Suman", 
        "id": "sg:person.01100526207.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100526207.07"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Colman", 
        "givenName": "Alan", 
        "id": "sg:person.0646726005.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646726005.33"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Sartipy", 
        "givenName": "Peter", 
        "id": "sg:person.0765614677.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765614677.89"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Matsui", 
        "givenName": "Sei-Ichi", 
        "type": "Person"
      }, 
      {
        "familyName": "Carpenter", 
        "givenName": "Melissa", 
        "id": "sg:person.01254176236.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254176236.61"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Gazdar", 
        "givenName": "Adi F", 
        "id": "sg:person.01351741346.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351741346.88"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Rao", 
        "givenName": "Mahendra", 
        "id": "sg:person.014201233047.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014201233047.52"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Chakravarti", 
        "givenName": "Aravinda", 
        "id": "sg:person.01221734637.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221734637.59"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nmeth718", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005603715", 
          "https://doi.org/10.1038/nmeth718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth718", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005603715", 
          "https://doi.org/10.1038/nmeth718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005956847", 
          "https://doi.org/10.1038/nbt922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005956847", 
          "https://doi.org/10.1038/nbt922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0404-381", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009913524", 
          "https://doi.org/10.1038/nbt0404-381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0404-381", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009913524", 
          "https://doi.org/10.1038/nbt0404-381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)64218-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013211523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.15.8681", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014931389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90186-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020604215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.2001.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027621591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1556", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029737108", 
          "https://doi.org/10.1038/ng1556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1556", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029737108", 
          "https://doi.org/10.1038/ng1556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1023085825042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030117586", 
          "https://doi.org/10.1023/a:1023085825042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.18.9821", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030175469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(99)01971-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031330354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.061013598", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033953973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/77083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036186944", 
          "https://doi.org/10.1038/77083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/77083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036186944", 
          "https://doi.org/10.1038/77083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1479-7364-1-4-287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036469651", 
          "https://doi.org/10.1186/1479-7364-1-4-287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/gcc.10122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037013084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.2228504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037363719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/gcc.10038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038382503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.20041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040495618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043439652", 
          "https://doi.org/10.1038/sj.onc.1208032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1208032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043439652", 
          "https://doi.org/10.1038/sj.onc.1208032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1582-4934.2002.tb00183.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045063409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/93.9.691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047096266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bc.2002.097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047694435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.10430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048489083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2004.13.585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050575585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.287.5460.2017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062568782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.197201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074949081"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-10", 
    "datePublishedReg": "2005-10-01", 
    "description": "Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. Nevertheless, these dividing cells, like other cells, probably undergo spontaneous mutation at a rate of 10(-9) per nucleotide. Because each mutant has only a few progeny, the overall biological properties of the cell culture are not altered unless a mutation provides a survival or growth advantage. Clonal evolution that leads to emergence of a dominant mutant genotype may potentially affect cellular phenotype as well. We assessed the genomic fidelity of paired early- and late-passage hESC lines in the course of tissue culture. Relative to early-passage lines, eight of nine late-passage hESC lines had one or more genomic alterations commonly observed in human cancers, including aberrations in copy number (45%), mitochondrial DNA sequence (22%) and gene promoter methylation (90%), although the latter was essentially restricted to 2 of 14 promoters examined. The observation that hESC lines maintained in vitro develop genetic and epigenetic alterations implies that periodic monitoring of these lines will be required before they are used in in vivo applications and that some late-passage hESC lines may be unusable for therapeutic purposes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/ng1631", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2722832", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2715362", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2708045", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2724504", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1103138", 
        "issn": [
          "1061-4036", 
          "1546-1718"
        ], 
        "name": "Nature Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "37"
      }
    ], 
    "name": "Genomic alterations in cultured human embryonic stem cells", 
    "pagination": "1099-1103", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3f1891e935da0a890e8696528061ce59cb2ebafd7105a7a226b5d94085c6fa71"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16142235"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9216904"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ng1631"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013244477"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ng1631", 
      "https://app.dimensions.ai/details/publication/pub.1013244477"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87112_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/ng/journal/v37/n10/full/ng1631.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng1631'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng1631'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng1631'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng1631'


 

This table displays all metadata directly associated to this object as RDF triples.

343 TRIPLES      21 PREDICATES      66 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ng1631 schema:about N050478079f3f437b83892891ff70269a
2 N0a496c86ddf44c04889c43fe912ae227
3 N0f589db54814427a8a7363c64a9b88c2
4 N2e824acb62f8475bb32215f1aed61329
5 N5399da9367e649fa99c2c17c5a84f09d
6 N6816b8f4b696429d91c952b47b221b03
7 Na4d9f77a55d0441e9959eacdd64a9314
8 Na8d1c8f430d442ff96450a8ec7e72895
9 Ncb572c443e9d4afb928b0832e644821d
10 Nea8a6701bbf5498d924d6c86030ce29a
11 Nf294361650d744f4b2334050a6e3b52d
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author N806ceb4d9f814fbe96a8c02a6e69aa40
15 schema:citation sg:pub.10.1023/a:1023085825042
16 sg:pub.10.1038/77083
17 sg:pub.10.1038/nbt0404-381
18 sg:pub.10.1038/nbt922
19 sg:pub.10.1038/ng1556
20 sg:pub.10.1038/nmeth718
21 sg:pub.10.1038/sj.onc.1208032
22 sg:pub.10.1186/1479-7364-1-4-287
23 https://doi.org/10.1002/dvdy.10430
24 https://doi.org/10.1002/gcc.10038
25 https://doi.org/10.1002/gcc.10122
26 https://doi.org/10.1002/ijc.20041
27 https://doi.org/10.1006/meth.2001.1262
28 https://doi.org/10.1016/0092-8674(90)90186-i
29 https://doi.org/10.1016/s0002-9440(10)64218-6
30 https://doi.org/10.1016/s0168-9525(99)01971-x
31 https://doi.org/10.1073/pnas.061013598
32 https://doi.org/10.1073/pnas.93.18.9821
33 https://doi.org/10.1073/pnas.96.15.8681
34 https://doi.org/10.1089/scd.2004.13.585
35 https://doi.org/10.1093/jnci/93.9.691
36 https://doi.org/10.1101/gr.197201
37 https://doi.org/10.1101/gr.2228504
38 https://doi.org/10.1111/j.1582-4934.2002.tb00183.x
39 https://doi.org/10.1126/science.287.5460.2017
40 https://doi.org/10.1515/bc.2002.097
41 schema:datePublished 2005-10
42 schema:datePublishedReg 2005-10-01
43 schema:description Cultured human embryonic stem cell (hESC) lines are an invaluable resource because they provide a uniform and stable genetic system for functional analyses and therapeutic applications. Nevertheless, these dividing cells, like other cells, probably undergo spontaneous mutation at a rate of 10(-9) per nucleotide. Because each mutant has only a few progeny, the overall biological properties of the cell culture are not altered unless a mutation provides a survival or growth advantage. Clonal evolution that leads to emergence of a dominant mutant genotype may potentially affect cellular phenotype as well. We assessed the genomic fidelity of paired early- and late-passage hESC lines in the course of tissue culture. Relative to early-passage lines, eight of nine late-passage hESC lines had one or more genomic alterations commonly observed in human cancers, including aberrations in copy number (45%), mitochondrial DNA sequence (22%) and gene promoter methylation (90%), although the latter was essentially restricted to 2 of 14 promoters examined. The observation that hESC lines maintained in vitro develop genetic and epigenetic alterations implies that periodic monitoring of these lines will be required before they are used in in vivo applications and that some late-passage hESC lines may be unusable for therapeutic purposes.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree false
47 schema:isPartOf N231dee40fe4d4119871d917d4d9cd9c2
48 N5192ce19d57047f9bf6bd4bd6b073906
49 sg:journal.1103138
50 schema:name Genomic alterations in cultured human embryonic stem cells
51 schema:pagination 1099-1103
52 schema:productId N080699b9bd8441b4b166832f3162e8a5
53 N297aa30c8f6a4729a96a26cb9b2d406d
54 N2a8f991d3fa5456fbfeb45211e55924d
55 N83cb381f41fb490b88b2483300984bd6
56 Ndc477e2a5f78499d99b8c52df09cb2c6
57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013244477
58 https://doi.org/10.1038/ng1631
59 schema:sdDatePublished 2019-04-11T12:26
60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
61 schema:sdPublisher Nad0bb658213d4f6a8d7a596887dc7f18
62 schema:url http://www.nature.com/ng/journal/v37/n10/full/ng1631.html
63 sgo:license sg:explorer/license/
64 sgo:sdDataset articles
65 rdf:type schema:ScholarlyArticle
66 N050478079f3f437b83892891ff70269a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Cell Line
68 rdf:type schema:DefinedTerm
69 N080699b9bd8441b4b166832f3162e8a5 schema:name pubmed_id
70 schema:value 16142235
71 rdf:type schema:PropertyValue
72 N0a496c86ddf44c04889c43fe912ae227 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Promoter Regions, Genetic
74 rdf:type schema:DefinedTerm
75 N0d68bc40d9464777ae4ff543abe617b2 rdf:first sg:person.0745313276.53
76 rdf:rest N0eab801a8aa746848165b21be42e0092
77 N0eab801a8aa746848165b21be42e0092 rdf:first sg:person.01104242053.89
78 rdf:rest Nd1813a7f7c7f45e383fd728bd8325af2
79 N0ec6f145544f4f978ba16908aaf926f1 rdf:first sg:person.01254176236.61
80 rdf:rest Nedfee60b3c0a454aa09dfc4a09b67ce5
81 N0f589db54814427a8a7363c64a9b88c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name DNA
83 rdf:type schema:DefinedTerm
84 N11fa984765e74c18be2c3ae94e42f53d rdf:first sg:person.01057672623.94
85 rdf:rest N78cdd3aadce64f239ce9e46924fb95d7
86 N15551cc8c92d4ec389a8d5be610f17b7 rdf:first sg:person.01156511547.21
87 rdf:rest N11fa984765e74c18be2c3ae94e42f53d
88 N1d5dcb5055794e60bc8fbaf6b5f20b0b rdf:first sg:person.015711065457.76
89 rdf:rest N1f8b2f384e91433384d7552cfbfe2d37
90 N1f8b2f384e91433384d7552cfbfe2d37 rdf:first N2fe92450707d4e4da2fcfc2c148f0d21
91 rdf:rest N53495f37a650402f9c13c779ffdf7559
92 N231dee40fe4d4119871d917d4d9cd9c2 schema:volumeNumber 37
93 rdf:type schema:PublicationVolume
94 N297aa30c8f6a4729a96a26cb9b2d406d schema:name readcube_id
95 schema:value 3f1891e935da0a890e8696528061ce59cb2ebafd7105a7a226b5d94085c6fa71
96 rdf:type schema:PropertyValue
97 N2a8f991d3fa5456fbfeb45211e55924d schema:name doi
98 schema:value 10.1038/ng1631
99 rdf:type schema:PropertyValue
100 N2e824acb62f8475bb32215f1aed61329 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Genome, Human
102 rdf:type schema:DefinedTerm
103 N2ec582b328ba47c8bfc4e6503107084c rdf:first sg:person.01304607577.06
104 rdf:rest N0d68bc40d9464777ae4ff543abe617b2
105 N2fe92450707d4e4da2fcfc2c148f0d21 schema:familyName Liu
106 schema:givenName Ying
107 rdf:type schema:Person
108 N461f208c7a504b9dae4466e446b25785 rdf:first sg:person.0756533042.24
109 rdf:rest N2ec582b328ba47c8bfc4e6503107084c
110 N496767d53bed4a358d32c58503b4a6b2 rdf:first sg:person.0646726005.33
111 rdf:rest N73d953eaf59f471c888cc69c1bf9d1c1
112 N5192ce19d57047f9bf6bd4bd6b073906 schema:issueNumber 10
113 rdf:type schema:PublicationIssue
114 N53495f37a650402f9c13c779ffdf7559 rdf:first sg:person.01026107356.71
115 rdf:rest N547dc88eab544a23ba29c8ae2083a52f
116 N5399da9367e649fa99c2c17c5a84f09d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Stem Cells
118 rdf:type schema:DefinedTerm
119 N547dc88eab544a23ba29c8ae2083a52f rdf:first sg:person.01205240623.21
120 rdf:rest N555db7a5153c4fa1b1dcaa7979bf2184
121 N555db7a5153c4fa1b1dcaa7979bf2184 rdf:first sg:person.0613004244.83
122 rdf:rest Ndba99001a6174809b666d16393213347
123 N566c652ee023476fa45f3d5c9e676bd2 schema:familyName Matsui
124 schema:givenName Sei-Ichi
125 rdf:type schema:Person
126 N5c13e855041e48c6af1bbb54d21b5497 rdf:first N566c652ee023476fa45f3d5c9e676bd2
127 rdf:rest N0ec6f145544f4f978ba16908aaf926f1
128 N5d48d84d48084bfc85a37b5891c586ef rdf:first sg:person.0754175020.89
129 rdf:rest N15551cc8c92d4ec389a8d5be610f17b7
130 N6816b8f4b696429d91c952b47b221b03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name DNA, Mitochondrial
132 rdf:type schema:DefinedTerm
133 N73d953eaf59f471c888cc69c1bf9d1c1 rdf:first sg:person.0765614677.89
134 rdf:rest N5c13e855041e48c6af1bbb54d21b5497
135 N78cdd3aadce64f239ce9e46924fb95d7 rdf:first sg:person.01100526207.07
136 rdf:rest N496767d53bed4a358d32c58503b4a6b2
137 N806ceb4d9f814fbe96a8c02a6e69aa40 rdf:first sg:person.01246576774.91
138 rdf:rest Nfe3d6ed4ce3b45828a15d886358bf5e5
139 N83cb381f41fb490b88b2483300984bd6 schema:name nlm_unique_id
140 schema:value 9216904
141 rdf:type schema:PropertyValue
142 Na4d9f77a55d0441e9959eacdd64a9314 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name DNA Methylation
144 rdf:type schema:DefinedTerm
145 Na8d1c8f430d442ff96450a8ec7e72895 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Cell Culture Techniques
147 rdf:type schema:DefinedTerm
148 Nad0bb658213d4f6a8d7a596887dc7f18 schema:name Springer Nature - SN SciGraph project
149 rdf:type schema:Organization
150 Naf30175a619e4950a64d04afa197818a rdf:first sg:person.01221734637.59
151 rdf:rest rdf:nil
152 Nc0edd793e5184e658c8462aa476be514 rdf:first sg:person.014201233047.52
153 rdf:rest Naf30175a619e4950a64d04afa197818a
154 Ncb572c443e9d4afb928b0832e644821d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Embryo, Mammalian
156 rdf:type schema:DefinedTerm
157 Nd1813a7f7c7f45e383fd728bd8325af2 rdf:first sg:person.01106342317.32
158 rdf:rest N1d5dcb5055794e60bc8fbaf6b5f20b0b
159 Ndba99001a6174809b666d16393213347 rdf:first sg:person.01244530113.11
160 rdf:rest N5d48d84d48084bfc85a37b5891c586ef
161 Ndc477e2a5f78499d99b8c52df09cb2c6 schema:name dimensions_id
162 schema:value pub.1013244477
163 rdf:type schema:PropertyValue
164 Nea8a6701bbf5498d924d6c86030ce29a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Mutation
166 rdf:type schema:DefinedTerm
167 Nedfee60b3c0a454aa09dfc4a09b67ce5 rdf:first sg:person.01351741346.88
168 rdf:rest Nc0edd793e5184e658c8462aa476be514
169 Nf294361650d744f4b2334050a6e3b52d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Humans
171 rdf:type schema:DefinedTerm
172 Nfe3d6ed4ce3b45828a15d886358bf5e5 rdf:first sg:person.01304013052.15
173 rdf:rest N461f208c7a504b9dae4466e446b25785
174 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
175 schema:name Biological Sciences
176 rdf:type schema:DefinedTerm
177 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
178 schema:name Genetics
179 rdf:type schema:DefinedTerm
180 sg:grant.2708045 http://pending.schema.org/fundedItem sg:pub.10.1038/ng1631
181 rdf:type schema:MonetaryGrant
182 sg:grant.2715362 http://pending.schema.org/fundedItem sg:pub.10.1038/ng1631
183 rdf:type schema:MonetaryGrant
184 sg:grant.2722832 http://pending.schema.org/fundedItem sg:pub.10.1038/ng1631
185 rdf:type schema:MonetaryGrant
186 sg:grant.2724504 http://pending.schema.org/fundedItem sg:pub.10.1038/ng1631
187 rdf:type schema:MonetaryGrant
188 sg:journal.1103138 schema:issn 1061-4036
189 1546-1718
190 schema:name Nature Genetics
191 rdf:type schema:Periodical
192 sg:person.01026107356.71 schema:familyName Brimble
193 schema:givenName Sandii N
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026107356.71
195 rdf:type schema:Person
196 sg:person.01057672623.94 schema:familyName Crook
197 schema:givenName Jeremy
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057672623.94
199 rdf:type schema:Person
200 sg:person.01100526207.07 schema:familyName Abraham
201 schema:givenName Suman
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100526207.07
203 rdf:type schema:Person
204 sg:person.01104242053.89 schema:familyName Kassauei
205 schema:givenName Keyaunoosh
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104242053.89
207 rdf:type schema:Person
208 sg:person.01106342317.32 schema:familyName Sui
209 schema:givenName Guoping
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106342317.32
211 rdf:type schema:Person
212 sg:person.01156511547.21 schema:familyName Freed
213 schema:givenName William J
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156511547.21
215 rdf:type schema:Person
216 sg:person.01205240623.21 schema:familyName Noaksson
217 schema:givenName Karin
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205240623.21
219 rdf:type schema:Person
220 sg:person.01221734637.59 schema:familyName Chakravarti
221 schema:givenName Aravinda
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221734637.59
223 rdf:type schema:Person
224 sg:person.01244530113.11 schema:familyName Schulz
225 schema:givenName Thomas C
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244530113.11
227 rdf:type schema:Person
228 sg:person.01246576774.91 schema:affiliation https://www.grid.ac/institutes/grid.21107.35
229 schema:familyName Maitra
230 schema:givenName Anirban
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246576774.91
232 rdf:type schema:Person
233 sg:person.01254176236.61 schema:familyName Carpenter
234 schema:givenName Melissa
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254176236.61
236 rdf:type schema:Person
237 sg:person.01304013052.15 schema:familyName Arking
238 schema:givenName Dan E
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304013052.15
240 rdf:type schema:Person
241 sg:person.01304607577.06 schema:familyName Ikeda
242 schema:givenName Morna
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304607577.06
244 rdf:type schema:Person
245 sg:person.01351741346.88 schema:familyName Gazdar
246 schema:givenName Adi F
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351741346.88
248 rdf:type schema:Person
249 sg:person.014201233047.52 schema:familyName Rao
250 schema:givenName Mahendra
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014201233047.52
252 rdf:type schema:Person
253 sg:person.015711065457.76 schema:familyName Cutler
254 schema:givenName David J
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015711065457.76
256 rdf:type schema:Person
257 sg:person.0613004244.83 schema:familyName Hyllner
258 schema:givenName Johan
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613004244.83
260 rdf:type schema:Person
261 sg:person.0646726005.33 schema:familyName Colman
262 schema:givenName Alan
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646726005.33
264 rdf:type schema:Person
265 sg:person.0745313276.53 schema:familyName Stastny
266 schema:givenName Victor
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745313276.53
268 rdf:type schema:Person
269 sg:person.0754175020.89 schema:familyName Zeng
270 schema:givenName Xianmin
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754175020.89
272 rdf:type schema:Person
273 sg:person.0756533042.24 schema:familyName Shivapurkar
274 schema:givenName Narayan
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756533042.24
276 rdf:type schema:Person
277 sg:person.0765614677.89 schema:familyName Sartipy
278 schema:givenName Peter
279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765614677.89
280 rdf:type schema:Person
281 sg:pub.10.1023/a:1023085825042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030117586
282 https://doi.org/10.1023/a:1023085825042
283 rdf:type schema:CreativeWork
284 sg:pub.10.1038/77083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036186944
285 https://doi.org/10.1038/77083
286 rdf:type schema:CreativeWork
287 sg:pub.10.1038/nbt0404-381 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009913524
288 https://doi.org/10.1038/nbt0404-381
289 rdf:type schema:CreativeWork
290 sg:pub.10.1038/nbt922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005956847
291 https://doi.org/10.1038/nbt922
292 rdf:type schema:CreativeWork
293 sg:pub.10.1038/ng1556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029737108
294 https://doi.org/10.1038/ng1556
295 rdf:type schema:CreativeWork
296 sg:pub.10.1038/nmeth718 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005603715
297 https://doi.org/10.1038/nmeth718
298 rdf:type schema:CreativeWork
299 sg:pub.10.1038/sj.onc.1208032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043439652
300 https://doi.org/10.1038/sj.onc.1208032
301 rdf:type schema:CreativeWork
302 sg:pub.10.1186/1479-7364-1-4-287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036469651
303 https://doi.org/10.1186/1479-7364-1-4-287
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1002/dvdy.10430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048489083
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1002/gcc.10038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038382503
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1002/gcc.10122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037013084
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1002/ijc.20041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040495618
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1006/meth.2001.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027621591
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1016/0092-8674(90)90186-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1020604215
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1016/s0002-9440(10)64218-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013211523
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1016/s0168-9525(99)01971-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031330354
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1073/pnas.061013598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033953973
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1073/pnas.93.18.9821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030175469
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1073/pnas.96.15.8681 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014931389
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1089/scd.2004.13.585 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050575585
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1093/jnci/93.9.691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047096266
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1101/gr.197201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074949081
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1101/gr.2228504 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037363719
334 rdf:type schema:CreativeWork
335 https://doi.org/10.1111/j.1582-4934.2002.tb00183.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045063409
336 rdf:type schema:CreativeWork
337 https://doi.org/10.1126/science.287.5460.2017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062568782
338 rdf:type schema:CreativeWork
339 https://doi.org/10.1515/bc.2002.097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047694435
340 rdf:type schema:CreativeWork
341 https://www.grid.ac/institutes/grid.21107.35 schema:alternateName Johns Hopkins University
342 schema:name McKusick-Nathans Institute of Genetic Medicine, Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
343 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...