A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-01

AUTHORS

Siu Sylvia Lee, Raymond Y.N. Lee, Andrew G. Fraser, Ravi S. Kamath, Julie Ahringer, Gary Ruvkun

ABSTRACT

We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity. More... »

PAGES

40-48

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng1056

DOI

http://dx.doi.org/10.1038/ng1056

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047240808

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12447374


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