A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-01

AUTHORS

Siu Sylvia Lee, Raymond Y.N. Lee, Andrew G. Fraser, Ravi S. Kamath, Julie Ahringer, Gary Ruvkun

ABSTRACT

We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity. More... »

PAGES

40-48

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng1056

DOI

http://dx.doi.org/10.1038/ng1056

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047240808

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12447374


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Helminth", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Testing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leucine-tRNA Ligase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Longevity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxygen Consumption", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Interference", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stress, Physiological", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Harvard University", 
          "id": "https://www.grid.ac/institutes/grid.38142.3c", 
          "name": [
            "Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Siu Sylvia", 
        "id": "sg:person.0774007237.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774007237.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Harvard University", 
          "id": "https://www.grid.ac/institutes/grid.38142.3c", 
          "name": [
            "Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Raymond Y.N.", 
        "id": "sg:person.01316216346.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316216346.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Wellcome Trust/Cancer Research UK Institute, University of Cambridge, Cambridge, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fraser", 
        "givenName": "Andrew G.", 
        "id": "sg:person.01237446422.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237446422.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Wellcome Trust/Cancer Research UK Institute, University of Cambridge, Cambridge, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kamath", 
        "givenName": "Ravi S.", 
        "id": "sg:person.0647452223.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647452223.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Wellcome Trust/Cancer Research UK Institute, University of Cambridge, Cambridge, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ahringer", 
        "givenName": "Julie", 
        "id": "sg:person.01100127223.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100127223.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Harvard University", 
          "id": "https://www.grid.ac/institutes/grid.38142.3c", 
          "name": [
            "Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ruvkun", 
        "givenName": "Gary", 
        "id": "sg:person.0600576602.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600576602.56"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1101/gad.867301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002133200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.22.13091", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003296828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35041700", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003389377", 
          "https://doi.org/10.1038/35041700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35041700", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003389377", 
          "https://doi.org/10.1038/35041700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.970902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004289431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(97)01299-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007290947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1534-5807(01)00071-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008099912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6603(08)60625-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009317923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.277.5328.942", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009449154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-0854.2001.1r008.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010229316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/366461a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011026745", 
          "https://doi.org/10.1038/366461a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1568-1637(02)00010-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013134094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0047-6374(01)00244-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013455457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.16.7540", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016184894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/21.3.221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016308427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.98.2.421", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016617560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.19.8905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020169117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.278.5341.1319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020198490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80391-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022062500"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/40194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022762380", 
          "https://doi.org/10.1038/40194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/40194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022762380", 
          "https://doi.org/10.1038/40194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00595-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024175913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35041687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028702240", 
          "https://doi.org/10.1038/35041687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35041687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028702240", 
          "https://doi.org/10.1038/35041687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0531-5565(99)00042-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028856185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0047-6374(01)00331-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030660359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00604-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030793805"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.2002.6394", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034191092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35042517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034980685", 
          "https://doi.org/10.1038/35042517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35042517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034980685", 
          "https://doi.org/10.1038/35042517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m104718200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035117734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00526-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037999010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.283.5407.1482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039626795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m103999200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040081735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/414807a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040179145", 
          "https://doi.org/10.1038/414807a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/414807a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040179145", 
          "https://doi.org/10.1038/414807a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382536a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044090038", 
          "https://doi.org/10.1038/382536a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051938768", 
          "https://doi.org/10.1038/nature00829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051938768", 
          "https://doi.org/10.1038/nature00829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0197-4580(99)00087-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052296043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.272.5264.1010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062553007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.275.5302.980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062555788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.290.5499.2137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062572477"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074365934", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.13.11.1385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074499627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074924588", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076959104", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082878716", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083083599", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-01", 
    "datePublishedReg": "2003-01-01", 
    "description": "We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/ng1056", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3626006", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1103138", 
        "issn": [
          "1061-4036", 
          "1546-1718"
        ], 
        "name": "Nature Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "33"
      }
    ], 
    "name": "A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity", 
    "pagination": "40-48", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e10a6bc9bf0aa003d14793d87dc482a034eb5d71bee9f0af89bb874222e728d4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12447374"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9216904"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ng1056"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1047240808"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ng1056", 
      "https://app.dimensions.ai/details/publication/pub.1047240808"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87088_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/ng1056"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng1056'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng1056'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng1056'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng1056'


 

This table displays all metadata directly associated to this object as RDF triples.

289 TRIPLES      21 PREDICATES      84 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ng1056 schema:about N6180911707f245ef978d6d4b5506f346
2 N7b0acd9871da40ecb3c20273236716c3
3 N7c1c08693507433a98c685e05b7c2c0b
4 Nbba724a1aa0644a48ae0049a667da878
5 Nbd3f656083fc4fbfb0b988c83d9b3f2a
6 Nbda9b13557634d49baee6893177c6c7c
7 Nd17e9b6dbac4485bb47f43d179a4af04
8 Ndcc805381a0c482e8720d001c976ab87
9 Nf6baad6d281e47979c5fe90be5748f86
10 Nf762f720281f4620a662428e984eb1e6
11 Nf76af16cd2d94adb840bb56319a3b809
12 Nfad2e4173df34a48b2811ee81f74883e
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author N18be64a3927f4427b93840098a218672
16 schema:citation sg:pub.10.1038/35041687
17 sg:pub.10.1038/35041700
18 sg:pub.10.1038/35042517
19 sg:pub.10.1038/366461a0
20 sg:pub.10.1038/382536a0
21 sg:pub.10.1038/40194
22 sg:pub.10.1038/414807a
23 sg:pub.10.1038/nature00829
24 https://app.dimensions.ai/details/publication/pub.1074365934
25 https://app.dimensions.ai/details/publication/pub.1074924588
26 https://app.dimensions.ai/details/publication/pub.1076959104
27 https://app.dimensions.ai/details/publication/pub.1082878716
28 https://app.dimensions.ai/details/publication/pub.1083083599
29 https://doi.org/10.1006/bbrc.2002.6394
30 https://doi.org/10.1016/s0047-6374(01)00244-5
31 https://doi.org/10.1016/s0047-6374(01)00331-1
32 https://doi.org/10.1016/s0079-6603(08)60625-x
33 https://doi.org/10.1016/s0168-9525(97)01299-7
34 https://doi.org/10.1016/s0197-4580(99)00087-1
35 https://doi.org/10.1016/s0531-5565(99)00042-x
36 https://doi.org/10.1016/s0960-9822(01)00526-7
37 https://doi.org/10.1016/s0960-9822(01)00595-4
38 https://doi.org/10.1016/s0960-9822(01)00604-2
39 https://doi.org/10.1016/s1097-2765(00)80391-3
40 https://doi.org/10.1016/s1534-5807(01)00071-5
41 https://doi.org/10.1016/s1568-1637(02)00010-7
42 https://doi.org/10.1034/j.1600-0854.2001.1r008.x
43 https://doi.org/10.1073/pnas.90.19.8905
44 https://doi.org/10.1073/pnas.92.16.7540
45 https://doi.org/10.1073/pnas.95.22.13091
46 https://doi.org/10.1073/pnas.98.2.421
47 https://doi.org/10.1074/jbc.m103999200
48 https://doi.org/10.1074/jbc.m104718200
49 https://doi.org/10.1093/emboj/21.3.221
50 https://doi.org/10.1096/fasebj.13.11.1385
51 https://doi.org/10.1101/gad.867301
52 https://doi.org/10.1101/gad.970902
53 https://doi.org/10.1126/science.272.5264.1010
54 https://doi.org/10.1126/science.275.5302.980
55 https://doi.org/10.1126/science.277.5328.942
56 https://doi.org/10.1126/science.278.5341.1319
57 https://doi.org/10.1126/science.283.5407.1482
58 https://doi.org/10.1126/science.290.5499.2137
59 schema:datePublished 2003-01
60 schema:datePublishedReg 2003-01-01
61 schema:description We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf N109d0faa6b4244ecb2edccb34e01000e
66 N8def0ce115ce4544a250876f344740f1
67 sg:journal.1103138
68 schema:name A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity
69 schema:pagination 40-48
70 schema:productId N7625f1d688524987a23b4e9bbc646938
71 Naa2b7d8a9375437aabbf3b41e8a084c4
72 Nbc2c3ca7192543fd8cfe29f67fffd6bc
73 Nd1f3a9a50f4440ef91bb380f48ffe9e4
74 Ndb2941f0048448a693fdebf3539fc21e
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047240808
76 https://doi.org/10.1038/ng1056
77 schema:sdDatePublished 2019-04-11T12:22
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher Nc3f503a3e8fa4aa6927dd150af8e0d07
80 schema:url http://www.nature.com/articles/ng1056
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N109d0faa6b4244ecb2edccb34e01000e schema:volumeNumber 33
85 rdf:type schema:PublicationVolume
86 N18be64a3927f4427b93840098a218672 rdf:first sg:person.0774007237.70
87 rdf:rest Ne05c7748978d4d629d9a9588d01d7acf
88 N5310e32ecb3149a89e9534831eb35f7b rdf:first sg:person.01100127223.14
89 rdf:rest Nee3a225561f1443396298a228189bd37
90 N6180911707f245ef978d6d4b5506f346 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name RNA Interference
92 rdf:type schema:DefinedTerm
93 N7625f1d688524987a23b4e9bbc646938 schema:name nlm_unique_id
94 schema:value 9216904
95 rdf:type schema:PropertyValue
96 N7b0acd9871da40ecb3c20273236716c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Genes, Helminth
98 rdf:type schema:DefinedTerm
99 N7c1c08693507433a98c685e05b7c2c0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Oxygen Consumption
101 rdf:type schema:DefinedTerm
102 N861d13f89cf748d1b205255c85841a8a rdf:first sg:person.0647452223.33
103 rdf:rest N5310e32ecb3149a89e9534831eb35f7b
104 N8def0ce115ce4544a250876f344740f1 schema:issueNumber 1
105 rdf:type schema:PublicationIssue
106 N9612f7d8ff9541b79b6e7d3601b65292 rdf:first sg:person.01237446422.95
107 rdf:rest N861d13f89cf748d1b205255c85841a8a
108 Naa2b7d8a9375437aabbf3b41e8a084c4 schema:name dimensions_id
109 schema:value pub.1047240808
110 rdf:type schema:PropertyValue
111 Nbba724a1aa0644a48ae0049a667da878 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Animals
113 rdf:type schema:DefinedTerm
114 Nbc2c3ca7192543fd8cfe29f67fffd6bc schema:name pubmed_id
115 schema:value 12447374
116 rdf:type schema:PropertyValue
117 Nbd3f656083fc4fbfb0b988c83d9b3f2a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Leucine-tRNA Ligase
119 rdf:type schema:DefinedTerm
120 Nbda9b13557634d49baee6893177c6c7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Mitochondria
122 rdf:type schema:DefinedTerm
123 Nc3f503a3e8fa4aa6927dd150af8e0d07 schema:name Springer Nature - SN SciGraph project
124 rdf:type schema:Organization
125 Nd17e9b6dbac4485bb47f43d179a4af04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Longevity
127 rdf:type schema:DefinedTerm
128 Nd1f3a9a50f4440ef91bb380f48ffe9e4 schema:name doi
129 schema:value 10.1038/ng1056
130 rdf:type schema:PropertyValue
131 Ndb2941f0048448a693fdebf3539fc21e schema:name readcube_id
132 schema:value e10a6bc9bf0aa003d14793d87dc482a034eb5d71bee9f0af89bb874222e728d4
133 rdf:type schema:PropertyValue
134 Ndcc805381a0c482e8720d001c976ab87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Caenorhabditis elegans
136 rdf:type schema:DefinedTerm
137 Ne05c7748978d4d629d9a9588d01d7acf rdf:first sg:person.01316216346.80
138 rdf:rest N9612f7d8ff9541b79b6e7d3601b65292
139 Nee3a225561f1443396298a228189bd37 rdf:first sg:person.0600576602.56
140 rdf:rest rdf:nil
141 Nf6baad6d281e47979c5fe90be5748f86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Stress, Physiological
143 rdf:type schema:DefinedTerm
144 Nf762f720281f4620a662428e984eb1e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Adenosine Triphosphate
146 rdf:type schema:DefinedTerm
147 Nf76af16cd2d94adb840bb56319a3b809 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Gene Expression Regulation
149 rdf:type schema:DefinedTerm
150 Nfad2e4173df34a48b2811ee81f74883e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Genetic Testing
152 rdf:type schema:DefinedTerm
153 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
154 schema:name Biological Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
157 schema:name Genetics
158 rdf:type schema:DefinedTerm
159 sg:grant.3626006 http://pending.schema.org/fundedItem sg:pub.10.1038/ng1056
160 rdf:type schema:MonetaryGrant
161 sg:journal.1103138 schema:issn 1061-4036
162 1546-1718
163 schema:name Nature Genetics
164 rdf:type schema:Periodical
165 sg:person.01100127223.14 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
166 schema:familyName Ahringer
167 schema:givenName Julie
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100127223.14
169 rdf:type schema:Person
170 sg:person.01237446422.95 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
171 schema:familyName Fraser
172 schema:givenName Andrew G.
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237446422.95
174 rdf:type schema:Person
175 sg:person.01316216346.80 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
176 schema:familyName Lee
177 schema:givenName Raymond Y.N.
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316216346.80
179 rdf:type schema:Person
180 sg:person.0600576602.56 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
181 schema:familyName Ruvkun
182 schema:givenName Gary
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600576602.56
184 rdf:type schema:Person
185 sg:person.0647452223.33 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
186 schema:familyName Kamath
187 schema:givenName Ravi S.
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647452223.33
189 rdf:type schema:Person
190 sg:person.0774007237.70 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
191 schema:familyName Lee
192 schema:givenName Siu Sylvia
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774007237.70
194 rdf:type schema:Person
195 sg:pub.10.1038/35041687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028702240
196 https://doi.org/10.1038/35041687
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/35041700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003389377
199 https://doi.org/10.1038/35041700
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/35042517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034980685
202 https://doi.org/10.1038/35042517
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/366461a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011026745
205 https://doi.org/10.1038/366461a0
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/382536a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044090038
208 https://doi.org/10.1038/382536a0
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/40194 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022762380
211 https://doi.org/10.1038/40194
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/414807a schema:sameAs https://app.dimensions.ai/details/publication/pub.1040179145
214 https://doi.org/10.1038/414807a
215 rdf:type schema:CreativeWork
216 sg:pub.10.1038/nature00829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051938768
217 https://doi.org/10.1038/nature00829
218 rdf:type schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1074365934 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1074924588 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1076959104 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082878716 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1083083599 schema:CreativeWork
224 https://doi.org/10.1006/bbrc.2002.6394 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034191092
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0047-6374(01)00244-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013455457
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0047-6374(01)00331-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030660359
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0079-6603(08)60625-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009317923
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s0168-9525(97)01299-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007290947
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s0197-4580(99)00087-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052296043
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/s0531-5565(99)00042-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028856185
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/s0960-9822(01)00526-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037999010
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0960-9822(01)00595-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024175913
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0960-9822(01)00604-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030793805
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s1097-2765(00)80391-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022062500
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s1534-5807(01)00071-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008099912
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s1568-1637(02)00010-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013134094
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1034/j.1600-0854.2001.1r008.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010229316
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1073/pnas.90.19.8905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020169117
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1073/pnas.92.16.7540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016184894
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1073/pnas.95.22.13091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003296828
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1073/pnas.98.2.421 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016617560
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1074/jbc.m103999200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040081735
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1074/jbc.m104718200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035117734
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1093/emboj/21.3.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016308427
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1096/fasebj.13.11.1385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074499627
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1101/gad.867301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002133200
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1101/gad.970902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004289431
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1126/science.272.5264.1010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062553007
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1126/science.275.5302.980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062555788
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1126/science.277.5328.942 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009449154
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.278.5341.1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020198490
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1126/science.283.5407.1482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039626795
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1126/science.290.5499.2137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062572477
283 rdf:type schema:CreativeWork
284 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
285 schema:name Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA.
286 rdf:type schema:Organization
287 https://www.grid.ac/institutes/grid.5335.0 schema:alternateName University of Cambridge
288 schema:name Wellcome Trust/Cancer Research UK Institute, University of Cambridge, Cambridge, UK.
289 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...