High resolution visual mapping of stretched DNA by fluorescent hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-09

AUTHORS

Irma Parra, Bradford Windle

ABSTRACT

We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi–colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a >200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures. More... »

PAGES

17-21

References to SciGraph publications

Journal

TITLE

Nature Genetics

ISSUE

1

VOLUME

5

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ng0993-17

    DOI

    http://dx.doi.org/10.1038/ng0993-17

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011197729

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8106079


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