Direct gene transfer of human CFTR into human bronchial epithelia of xenografts with E1–deleted adenoviruses View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1993-05

AUTHORS

J F Engelhardt, Y Yang, L D Stratford-Perricaudet, E D Allen, K Kozarsky, M Perricaudet, J R Yankaskas, J M Wilson

ABSTRACT

We describe the use of a human bronchial xenograft model for studying the efficiency and biology of in vivo gene transfer into human bronchial epithelia with recombinant E1 deleted adenoviruses. All cell types in the surface epithelium except basal cells efficiently expressed the adenoviral transduced recombinant genes, lacZ and CFTR, for 3-5 weeks. Stable transgene expression was associated with high level expression of the early adenoviral gene, E2a, in a subset of transgene expressing cells and virtually undetectable expression of the late adenoviral genes encoding the structural proteins, hexon and fiber. These studies begin to address important issues that relate to safety and in vivo efficacy of recombinant adenoviruses for gene delivery into the human airway. More... »

PAGES

27-34

Journal

TITLE

Nature Genetics

ISSUE

1

VOLUME

4

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ng0593-27

    DOI

    http://dx.doi.org/10.1038/ng0593-27

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7685651


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