Ontology type: schema:ScholarlyArticle
1994-01
AUTHORSDeanna M. Church, Christy J. Stotler, Joni L. Rutter, Jill R. Murrell, James A. Trofatter, Alan J. Buckler
ABSTRACTModifications to exon amplification have been instituted that increase its speed, efficiency and reliability. Exons were isolated from target human or mouse genomic DNA sources ranging from 30 kilobases (kb) to 3 megabases (Mb) in complexity. The efficiency was dependent upon the amount of input DNA, and ranged from isolation of an exon for every 20 kb to an exon for every 80 kb of target genomic DNA. In these studies, several novel genes and a smaller number of genes isolated previously that reside on human chromosome 9 have been identified. These results indicate that exon amplification is presently adaptable to large scale isolation of exons from complex sources of genomic DNA. More... »
PAGES98-105
http://scigraph.springernature.com/pub.10.1038/ng0194-98
DOIhttp://dx.doi.org/10.1038/ng0194-98
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1053494375
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8136842
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carboxylesterase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Carboxylic Ester Hydrolases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromosomes, Human, Pair 9",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Primers",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Exons",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Amplification",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Techniques",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mice",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polymerase Chain Reaction",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Church",
"givenName": "Deanna M.",
"id": "sg:person.0776776316.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776776316.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Stotler",
"givenName": "Christy J.",
"id": "sg:person.01245536410.37",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245536410.37"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Rutter",
"givenName": "Joni L.",
"id": "sg:person.0634026542.94",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634026542.94"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Murrell",
"givenName": "Jill R.",
"id": "sg:person.0742053170.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742053170.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Trofatter",
"givenName": "James A.",
"id": "sg:person.01363307460.30",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363307460.30"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA",
"id": "http://www.grid.ac/institutes/grid.32224.35",
"name": [
"Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
],
"type": "Organization"
},
"familyName": "Buckler",
"givenName": "Alan J.",
"id": "sg:person.0725766526.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725766526.54"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/ng0792-278",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036454728",
"https://doi.org/10.1038/ng0792-278"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/321209a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022096390",
"https://doi.org/10.1038/321209a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng0193-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027335691",
"https://doi.org/10.1038/ng0193-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng0493-292",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027106761",
"https://doi.org/10.1038/ng0493-292"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/322275a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036441248",
"https://doi.org/10.1038/322275a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng1192-173",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034300680",
"https://doi.org/10.1038/ng1192-173"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng1192-180",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005408376",
"https://doi.org/10.1038/ng1192-180"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/323646a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015578451",
"https://doi.org/10.1038/323646a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/347256a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028569139",
"https://doi.org/10.1038/347256a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/322279a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031233522",
"https://doi.org/10.1038/322279a0"
],
"type": "CreativeWork"
}
],
"datePublished": "1994-01",
"datePublishedReg": "1994-01-01",
"description": "Modifications to exon amplification have been instituted that increase its speed, efficiency and reliability. Exons were isolated from target human or mouse genomic DNA sources ranging from 30 kilobases (kb) to 3 megabases (Mb) in complexity. The efficiency was dependent upon the amount of input DNA, and ranged from isolation of an exon for every 20 kb to an exon for every 80 kb of target genomic DNA. In these studies, several novel genes and a smaller number of genes isolated previously that reside on human chromosome 9 have been identified. These results indicate that exon amplification is presently adaptable to large scale isolation of exons from complex sources of genomic DNA.",
"genre": "article",
"id": "sg:pub.10.1038/ng0194-98",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1103138",
"issn": [
"1061-4036",
"1546-1718"
],
"name": "Nature Genetics",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "6"
}
],
"keywords": [
"genomic DNA",
"exon amplification",
"isolation of genes",
"mammalian genomic DNA",
"human chromosome 9",
"target genomic DNA",
"novel genes",
"genomic DNA sources",
"chromosome 9",
"exons",
"DNA source",
"large-scale isolation",
"genes",
"input DNA",
"DNA",
"kb",
"scale isolation",
"amplification",
"isolation",
"megabases",
"kilobases",
"resides",
"small number",
"humans",
"modification",
"complex sources",
"target human",
"source",
"number",
"amount",
"study",
"efficiency",
"complexity",
"results",
"speed",
"reliability"
],
"name": "Isolation of genes from complex sources of mammalian genomic DNA using exon amplification",
"pagination": "98-105",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1053494375"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/ng0194-98"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"8136842"
]
}
],
"sameAs": [
"https://doi.org/10.1038/ng0194-98",
"https://app.dimensions.ai/details/publication/pub.1053494375"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:51",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_256.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/ng0194-98"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'
This table displays all metadata directly associated to this object as RDF triples.
228 TRIPLES
21 PREDICATES
86 URIs
68 LITERALS
21 BLANK NODES