Isolation of genes from complex sources of mammalian genomic DNA using exon amplification View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-01

AUTHORS

Deanna M. Church, Christy J. Stotler, Joni L. Rutter, Jill R. Murrell, James A. Trofatter, Alan J. Buckler

ABSTRACT

Modifications to exon amplification have been instituted that increase its speed, efficiency and reliability. Exons were isolated from target human or mouse genomic DNA sources ranging from 30 kilobases (kb) to 3 megabases (Mb) in complexity. The efficiency was dependent upon the amount of input DNA, and ranged from isolation of an exon for every 20 kb to an exon for every 80 kb of target genomic DNA. In these studies, several novel genes and a smaller number of genes isolated previously that reside on human chromosome 9 have been identified. These results indicate that exon amplification is presently adaptable to large scale isolation of exons from complex sources of genomic DNA. More... »

PAGES

98-105

Journal

TITLE

Nature Genetics

ISSUE

1

VOLUME

6

Related Patents

  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Brain
  • Hereditary Hemochromatosis Gene
  • A Long Qt Syndrome Gene Which Encodes Kvlqt1 And Its Association With Mink
  • Method For Identifying Mutants And Molecules
  • Ataxia-Telangiectasai Gene
  • Immunoglobulin Superfamily Receptor Translocation Associated (Irta) Protein Specific Immunoglobulin For Use In Treatment And Prevention Of Cancer And Cell Proliferative Disorders
  • Eukaryotic Cells For Use As Tools In Genetic Engineering
  • Atg-1117 (Aif-3), A Homolog Of Allograft Inflammatory Factor-1/Rc-9
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Ajusted Nucleic Acid Sequence; For Detection Of Genome Adjustments And Preferential Genetic Disorders
  • Mutated Proteins Associated With Ataxia-Telangiectasia
  • Isolation Of Five Novel Genes Coding For New Fc Receptors-Type Melanoma Involved In The Pathogenesis Of Lymphoma/Melanoma
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Hela Cells Or Other Human Cervical Epithelial Cells
  • Isolated Antibody Directed To An Immunoglobulin Superfamily Receptor Translocation Associated, Irta, Protein; Capable Of Hybridizing With A Unique Sequence Within The Sequence Of An Isolated Rna Encoding Human Irta Protein; The Nucleic Acid Molecule Is Labeled With A Detectable Marker
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Nucleotide Sequences Associated With Genetic Disorders; For The Detection And Diagnosis Of Preferential Genetic Disorders
  • Genetic Engineering Using Eukaryotic Cells Containing Enhancers, Transcriptional Regulatory Sequences And Splicers; Gene Expression
  • Novel Human Chromosome 16 Genes, Compositions, Methods Of Making And Using Same
  • By Illegitimate Recombination Of A Regulatory Sequence; Identification, Activation, Isolation, And/Or Expression Of Genes Without Need For Target Sequence During Integration
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Isolation Of Five Novel Genes Coding For New Fc Receptors-Type Melanoma Involved In The Pathogenesis Of Lymphoma/Melanoma
  • Isolation Of Five Novel Genes Coding For New Fc Receptors-Type Melanoma Involved In The Pathogenesis Of Lymphoma/Melanoma
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Fetal Liver
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Cdnas Associated With Ataxia-Telangiectasia
  • Megabase Transcript Map: Novel Sequences And Antibodies Thereto
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Brain
  • Nucleic Acid That Encodes A Human Netrin Polypeptide Important In Axon Guidance; For Hybridization Probes; For Assays To Screen For Compounds Which Bind To Polypeptide
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Breast And Bt 474 Cells
  • Determining Genotype Of A Polymorphic Site In The Hereditary Hemochromatosis Gene
  • Hereditary Hemochromatosis Gene
  • Isolation Of Five Novel Genes Coding For New Fc Receptors-Type Melanoma Involved In The Pathogenesis Of Lymphoma/Melanoma
  • Isolation, Purification, Cloned Nucleic Acid Sequence With Mutation Causing Protein Truncation Or No Initiation; Detecting Carriers By Analysis Of Nucleic Acids Isolated From Patients With Hereditary Muscular Disorders
  • Producing A Mutant Hh Polypeptide By Transforming Isolated Host Cells With A Vector Capable Of Expressing The Polypeptide From The Nucleic Acid Sequence; Diagnosis, Prenatal Screening And Diagnosis, And Therapies Of Blood Disorders
  • Amino Acid Sequence Of Polypeptide Involved In Axon Outgrowth
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Lung
  • Method Of Isolating Exonic Gene Segments Of Eukaryotic Genes, Exon Trapping Vectors For Use Therein
  • Genes Mapping In The Digeorge And Velocardiofacial Syndrome Minimal Critical Region
  • Reducing Binding Of Transferrin To Receptor By Contacting It With Microglobulin Polypeptide; Hereditary Hemochromatosis
  • Compositions And Method For Non-Targeted Activation Of Endogenous Genes
  • Isolation Of Five Novel Genes Coding For New Fc Receptors-Type Melanoma Involved In The Pathogenesis Of Lymphoma/Melanoma
  • Treating Hemachromatosis Comprising Administering An Hfe Polypeptide, Fragments, Or A Complex With Beta -2-Microglobulin; Genetic Disorders
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Lung
  • Compositions And Methods For Non-Targeted Activation Of Endogenous Genes
  • Human Genome-Derived Single Exon Nucleic Acid Probes Useful For Analysis Of Gene Expression In Human Breast And Bt 474 Cells
  • Polymorphisms In The Region Of The Human Hemochromatosis Gene
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ng0194-98

    DOI

    http://dx.doi.org/10.1038/ng0194-98

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1053494375

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8136842


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carboxylesterase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carboxylic Ester Hydrolases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromosomes, Human, Pair 9", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Primers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Exons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Amplification", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Church", 
            "givenName": "Deanna M.", 
            "id": "sg:person.0776776316.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776776316.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stotler", 
            "givenName": "Christy J.", 
            "id": "sg:person.01245536410.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245536410.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rutter", 
            "givenName": "Joni L.", 
            "id": "sg:person.0634026542.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634026542.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Murrell", 
            "givenName": "Jill R.", 
            "id": "sg:person.0742053170.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742053170.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Trofatter", 
            "givenName": "James A.", 
            "id": "sg:person.01363307460.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363307460.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.32224.35", 
              "name": [
                "Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Buckler", 
            "givenName": "Alan J.", 
            "id": "sg:person.0725766526.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725766526.54"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/ng0792-278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036454728", 
              "https://doi.org/10.1038/ng0792-278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/321209a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022096390", 
              "https://doi.org/10.1038/321209a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0193-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027335691", 
              "https://doi.org/10.1038/ng0193-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0493-292", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027106761", 
              "https://doi.org/10.1038/ng0493-292"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/322275a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036441248", 
              "https://doi.org/10.1038/322275a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1192-173", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034300680", 
              "https://doi.org/10.1038/ng1192-173"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1192-180", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005408376", 
              "https://doi.org/10.1038/ng1192-180"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/323646a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015578451", 
              "https://doi.org/10.1038/323646a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/347256a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028569139", 
              "https://doi.org/10.1038/347256a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/322279a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031233522", 
              "https://doi.org/10.1038/322279a0"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-01", 
        "datePublishedReg": "1994-01-01", 
        "description": "Modifications to exon amplification have been instituted that increase its speed, efficiency and reliability. Exons were isolated from target human or mouse genomic DNA sources ranging from 30 kilobases (kb) to 3 megabases (Mb) in complexity. The efficiency was dependent upon the amount of input DNA, and ranged from isolation of an exon for every 20 kb to an exon for every 80 kb of target genomic DNA. In these studies, several novel genes and a smaller number of genes isolated previously that reside on human chromosome 9 have been identified. These results indicate that exon amplification is presently adaptable to large scale isolation of exons from complex sources of genomic DNA.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/ng0194-98", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1103138", 
            "issn": [
              "1061-4036", 
              "1546-1718"
            ], 
            "name": "Nature Genetics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "6"
          }
        ], 
        "keywords": [
          "genomic DNA", 
          "exon amplification", 
          "isolation of genes", 
          "mammalian genomic DNA", 
          "human chromosome 9", 
          "target genomic DNA", 
          "novel genes", 
          "genomic DNA sources", 
          "chromosome 9", 
          "exons", 
          "DNA source", 
          "large-scale isolation", 
          "genes", 
          "input DNA", 
          "DNA", 
          "kb", 
          "scale isolation", 
          "amplification", 
          "isolation", 
          "megabases", 
          "kilobases", 
          "resides", 
          "small number", 
          "humans", 
          "modification", 
          "complex sources", 
          "target human", 
          "source", 
          "number", 
          "amount", 
          "study", 
          "efficiency", 
          "complexity", 
          "results", 
          "speed", 
          "reliability"
        ], 
        "name": "Isolation of genes from complex sources of mammalian genomic DNA using exon amplification", 
        "pagination": "98-105", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1053494375"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ng0194-98"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8136842"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ng0194-98", 
          "https://app.dimensions.ai/details/publication/pub.1053494375"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-08-04T16:51", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_256.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/ng0194-98"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng0194-98'


     

    This table displays all metadata directly associated to this object as RDF triples.

    228 TRIPLES      21 PREDICATES      86 URIs      68 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ng0194-98 schema:about N1c6d4a557ba5423bae0319ffde8d66eb
    2 N2d3c4541e7f0451b9c81c0f808f27030
    3 N4d8c1967ac974608b96940d7e3ddfb60
    4 N6a79698e9ea2439ba9c04466c832b3ca
    5 N709ca40e15904b7ba8c6aa0122c18b4c
    6 N78201eed27d5462aa43f3383ef81a62a
    7 N89c62c096e2e45dbbeaae35d69d64d78
    8 N9d82b34947364f0b827ad2fee5e4e302
    9 Na8fbde65578a436c834b29c284b5015d
    10 Nc279da2a665e460fb0689ffc666c8435
    11 Nd556072233ad41fb8d7421106261b6ec
    12 Nd8859dfdf9404792a621822c432d0728
    13 Neb99a285f8e547deac9b1d2927e12215
    14 Nf8bf54275f96422585ccb0d1d57e60e4
    15 anzsrc-for:06
    16 anzsrc-for:0604
    17 schema:author N599c5041905b40f491be863e435053e1
    18 schema:citation sg:pub.10.1038/321209a0
    19 sg:pub.10.1038/322275a0
    20 sg:pub.10.1038/322279a0
    21 sg:pub.10.1038/323646a0
    22 sg:pub.10.1038/347256a0
    23 sg:pub.10.1038/ng0193-7
    24 sg:pub.10.1038/ng0493-292
    25 sg:pub.10.1038/ng0792-278
    26 sg:pub.10.1038/ng1192-173
    27 sg:pub.10.1038/ng1192-180
    28 schema:datePublished 1994-01
    29 schema:datePublishedReg 1994-01-01
    30 schema:description Modifications to exon amplification have been instituted that increase its speed, efficiency and reliability. Exons were isolated from target human or mouse genomic DNA sources ranging from 30 kilobases (kb) to 3 megabases (Mb) in complexity. The efficiency was dependent upon the amount of input DNA, and ranged from isolation of an exon for every 20 kb to an exon for every 80 kb of target genomic DNA. In these studies, several novel genes and a smaller number of genes isolated previously that reside on human chromosome 9 have been identified. These results indicate that exon amplification is presently adaptable to large scale isolation of exons from complex sources of genomic DNA.
    31 schema:genre article
    32 schema:isAccessibleForFree false
    33 schema:isPartOf N067a3ecca16f4675bac147f5d1784488
    34 Nbeab614c09d44f2fbc016143f8ad86c2
    35 sg:journal.1103138
    36 schema:keywords DNA
    37 DNA source
    38 amount
    39 amplification
    40 chromosome 9
    41 complex sources
    42 complexity
    43 efficiency
    44 exon amplification
    45 exons
    46 genes
    47 genomic DNA
    48 genomic DNA sources
    49 human chromosome 9
    50 humans
    51 input DNA
    52 isolation
    53 isolation of genes
    54 kb
    55 kilobases
    56 large-scale isolation
    57 mammalian genomic DNA
    58 megabases
    59 modification
    60 novel genes
    61 number
    62 reliability
    63 resides
    64 results
    65 scale isolation
    66 small number
    67 source
    68 speed
    69 study
    70 target genomic DNA
    71 target human
    72 schema:name Isolation of genes from complex sources of mammalian genomic DNA using exon amplification
    73 schema:pagination 98-105
    74 schema:productId N0e01bc13502f4b48ab597c5940f99349
    75 N5a92f3709e614712bd56ef6b39f337f2
    76 N9015af827cf64ba59e7adc1e4fa3b7fa
    77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053494375
    78 https://doi.org/10.1038/ng0194-98
    79 schema:sdDatePublished 2022-08-04T16:51
    80 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    81 schema:sdPublisher N8e2751d3b950495cac63fbaee4c82eb3
    82 schema:url https://doi.org/10.1038/ng0194-98
    83 sgo:license sg:explorer/license/
    84 sgo:sdDataset articles
    85 rdf:type schema:ScholarlyArticle
    86 N067a3ecca16f4675bac147f5d1784488 schema:volumeNumber 6
    87 rdf:type schema:PublicationVolume
    88 N0e01bc13502f4b48ab597c5940f99349 schema:name dimensions_id
    89 schema:value pub.1053494375
    90 rdf:type schema:PropertyValue
    91 N1c6d4a557ba5423bae0319ffde8d66eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Exons
    93 rdf:type schema:DefinedTerm
    94 N2d3c4541e7f0451b9c81c0f808f27030 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Genetic Techniques
    96 rdf:type schema:DefinedTerm
    97 N3986cac9ac53403ba3f5d3dd5c56f1a7 rdf:first sg:person.0742053170.51
    98 rdf:rest Nb41e9b58b930488fbb1977cc41cf99ac
    99 N4d8c1967ac974608b96940d7e3ddfb60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Animals
    101 rdf:type schema:DefinedTerm
    102 N599c5041905b40f491be863e435053e1 rdf:first sg:person.0776776316.41
    103 rdf:rest Nda9fe3522cae497891f3a0d663b0e915
    104 N5a92f3709e614712bd56ef6b39f337f2 schema:name doi
    105 schema:value 10.1038/ng0194-98
    106 rdf:type schema:PropertyValue
    107 N6a79698e9ea2439ba9c04466c832b3ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Gene Amplification
    109 rdf:type schema:DefinedTerm
    110 N709ca40e15904b7ba8c6aa0122c18b4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Base Sequence
    112 rdf:type schema:DefinedTerm
    113 N78201eed27d5462aa43f3383ef81a62a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name DNA
    115 rdf:type schema:DefinedTerm
    116 N7c252aa0ca304f04b93e514ea070d6de rdf:first sg:person.0725766526.54
    117 rdf:rest rdf:nil
    118 N89c62c096e2e45dbbeaae35d69d64d78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Chromosomes, Human, Pair 9
    120 rdf:type schema:DefinedTerm
    121 N8e2751d3b950495cac63fbaee4c82eb3 schema:name Springer Nature - SN SciGraph project
    122 rdf:type schema:Organization
    123 N9015af827cf64ba59e7adc1e4fa3b7fa schema:name pubmed_id
    124 schema:value 8136842
    125 rdf:type schema:PropertyValue
    126 N919a07192d184cfea1a5cf83995057b2 rdf:first sg:person.0634026542.94
    127 rdf:rest N3986cac9ac53403ba3f5d3dd5c56f1a7
    128 N9d82b34947364f0b827ad2fee5e4e302 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name DNA Primers
    130 rdf:type schema:DefinedTerm
    131 Na8fbde65578a436c834b29c284b5015d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Molecular Sequence Data
    133 rdf:type schema:DefinedTerm
    134 Nb41e9b58b930488fbb1977cc41cf99ac rdf:first sg:person.01363307460.30
    135 rdf:rest N7c252aa0ca304f04b93e514ea070d6de
    136 Nbeab614c09d44f2fbc016143f8ad86c2 schema:issueNumber 1
    137 rdf:type schema:PublicationIssue
    138 Nc279da2a665e460fb0689ffc666c8435 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Carboxylic Ester Hydrolases
    140 rdf:type schema:DefinedTerm
    141 Nd556072233ad41fb8d7421106261b6ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Polymerase Chain Reaction
    143 rdf:type schema:DefinedTerm
    144 Nd8859dfdf9404792a621822c432d0728 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Humans
    146 rdf:type schema:DefinedTerm
    147 Nda9fe3522cae497891f3a0d663b0e915 rdf:first sg:person.01245536410.37
    148 rdf:rest N919a07192d184cfea1a5cf83995057b2
    149 Neb99a285f8e547deac9b1d2927e12215 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Carboxylesterase
    151 rdf:type schema:DefinedTerm
    152 Nf8bf54275f96422585ccb0d1d57e60e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Mice
    154 rdf:type schema:DefinedTerm
    155 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    156 schema:name Biological Sciences
    157 rdf:type schema:DefinedTerm
    158 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    159 schema:name Genetics
    160 rdf:type schema:DefinedTerm
    161 sg:journal.1103138 schema:issn 1061-4036
    162 1546-1718
    163 schema:name Nature Genetics
    164 schema:publisher Springer Nature
    165 rdf:type schema:Periodical
    166 sg:person.01245536410.37 schema:affiliation grid-institutes:grid.32224.35
    167 schema:familyName Stotler
    168 schema:givenName Christy J.
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245536410.37
    170 rdf:type schema:Person
    171 sg:person.01363307460.30 schema:affiliation grid-institutes:grid.32224.35
    172 schema:familyName Trofatter
    173 schema:givenName James A.
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363307460.30
    175 rdf:type schema:Person
    176 sg:person.0634026542.94 schema:affiliation grid-institutes:grid.32224.35
    177 schema:familyName Rutter
    178 schema:givenName Joni L.
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634026542.94
    180 rdf:type schema:Person
    181 sg:person.0725766526.54 schema:affiliation grid-institutes:grid.32224.35
    182 schema:familyName Buckler
    183 schema:givenName Alan J.
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725766526.54
    185 rdf:type schema:Person
    186 sg:person.0742053170.51 schema:affiliation grid-institutes:grid.32224.35
    187 schema:familyName Murrell
    188 schema:givenName Jill R.
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742053170.51
    190 rdf:type schema:Person
    191 sg:person.0776776316.41 schema:affiliation grid-institutes:grid.32224.35
    192 schema:familyName Church
    193 schema:givenName Deanna M.
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776776316.41
    195 rdf:type schema:Person
    196 sg:pub.10.1038/321209a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022096390
    197 https://doi.org/10.1038/321209a0
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1038/322275a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036441248
    200 https://doi.org/10.1038/322275a0
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1038/322279a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031233522
    203 https://doi.org/10.1038/322279a0
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1038/323646a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015578451
    206 https://doi.org/10.1038/323646a0
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1038/347256a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028569139
    209 https://doi.org/10.1038/347256a0
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1038/ng0193-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027335691
    212 https://doi.org/10.1038/ng0193-7
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1038/ng0493-292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027106761
    215 https://doi.org/10.1038/ng0493-292
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/ng0792-278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036454728
    218 https://doi.org/10.1038/ng0792-278
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/ng1192-173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034300680
    221 https://doi.org/10.1038/ng1192-173
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/ng1192-180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005408376
    224 https://doi.org/10.1038/ng1192-180
    225 rdf:type schema:CreativeWork
    226 grid-institutes:grid.32224.35 schema:alternateName Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA
    227 schema:name Molecular Neurogenetics Unit, Massachusetts General Hospital, and Department of Neurology, Harvard Medical School, 02114, Boston, Massachusetts, USA
    228 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...