A new bacteriophage P1–derived vector for the propagation of large human DNA fragments View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-01

AUTHORS

Panayiotis A. loannou, Chris T. Amemiya, Jeffrey Garnes, Peter M. Kroisel, Hiroaki Shizuya, Chira Chen, Mark A. Batzer, Pieter J. de Jong

ABSTRACT

We have designed a P1 vector (pCYPAC–1) for the introduction of recombinant DNA into E. coli using electroporation procedures. The new cloning system, P1–derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130–150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes. More... »

PAGES

84-89

Journal

TITLE

Nature Genetics

ISSUE

1

VOLUME

6

Related Patents

  • Methods For Increasing Production Of Ifn-Gamma In Natural Killer Cells Expressing Llt1 Receptor
  • Novel Vectors For Improving Cloning And Expression In Low Copy Number Plasmids
  • Human 1p36 Homozygosity Deletion Domain
  • Nucleic Acids, Kits, And Methods For The Diagnosis, Prognosis And Treatment Of Glaucoma And Related Disorders
  • Method Of Detecting Expanded Cag Repeat Region In Spinocerebellar Ataxia-2 Gene
  • Artificial Ubiquitous Chromatin Opening Elements (Ucoe)
  • A Polynucleotide Comprising A Ubiquitous Chromatin Opening Element (Ucoe)
  • Polynucleotides For Diagnosing Congenital Heart Disease Resulting From Down Syndrome
  • Chromosome-Based Platforms
  • Nucleic Acid Encoding Ds-Cam Proteins And Products Related Thereto
  • Cloning And/Or Sequencing Vector
  • A Polypeptide Encoding Protein; Therapy And Diagnosis
  • Vectors For Improving Cloning And Expression In Low Copy Number Plasmids
  • Comprises Nucleotide Sequences Neuronal Proteins For Diagnosis And Treatment Of Neurodegenerative Diseases
  • Enhanced Packaging Of Herpes Virus Amplicons And Generation Of Recombinant Virus Vectors
  • Fungal Artificial Chromosomes, Compositions, Methods And Uses Therefor
  • Microorganism Genomics, Compositions And Methods Related Thereto
  • Gene Therapy
  • Diagnosis By Detecting Mutants In The Promoter Region Of The Tigr Gene Using Nucleic Acid Hybridization
  • Human 1p36 Homozygosity Deletion Domain
  • Microorganism Genomics, Compositions And Methods Related Thereto
  • Nucleic Acid Sequences; Hybridization Probes; Primers; Oligonucleotides; Used In The Detection And Diagnosis Of Degenerative Neurological Disorders Of The Nervous System
  • Method Of Isolating Exonic Gene Segments Of Eukaryotic Genes, Exon Trapping Vectors For Use Therein
  • Genetic Engineering; Vectors For Supplying Nucleic Acids
  • Nucleic Acids, Kits And Methods For The Diagnosis, Prognosis And Treatment Of Glaucoma And Related Disorders
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ng0194-84

    DOI

    http://dx.doi.org/10.1038/ng0194-84

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027542612

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8136839


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteriophage P1", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Recombinant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Library", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "In Situ Hybridization, Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "The Cyprus Institute of Neurology and Genetics, P.O. Box 3462, Nicosia, Cyprus", 
              "id": "http://www.grid.ac/institutes/grid.417705.0", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
                "The Cyprus Institute of Neurology and Genetics, P.O. Box 3462, Nicosia, Cyprus"
              ], 
              "type": "Organization"
            }, 
            "familyName": "loannou", 
            "givenName": "Panayiotis A.", 
            "id": "sg:person.013566425337.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013566425337.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Human Genetics, Boston University School of Medicine, 80 East Concord St, 02118, Boston, Massachusetts, USA", 
              "id": "http://www.grid.ac/institutes/grid.189504.1", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
                "Center for Human Genetics, Boston University School of Medicine, 80 East Concord St, 02118, Boston, Massachusetts, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amemiya", 
            "givenName": "Chris T.", 
            "id": "sg:person.01132314305.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132314305.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.250008.f", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Garnes", 
            "givenName": "Jeffrey", 
            "id": "sg:person.01167721010.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167721010.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Medical Biology and Human Genetics, Harrachgasse 21/8, A-8010, Graz, Austria", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
                "Department of Medical Biology and Human Genetics, Harrachgasse 21/8, A-8010, Graz, Austria"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kroisel", 
            "givenName": "Peter M.", 
            "id": "sg:person.0632331622.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632331622.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.20861.3d", 
              "name": [
                "Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shizuya", 
            "givenName": "Hiroaki", 
            "id": "sg:person.01233556444.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233556444.33"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA", 
              "id": "http://www.grid.ac/institutes/grid.240614.5", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
                "Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Chira", 
            "id": "sg:person.01232642324.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232642324.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.250008.f", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Batzer", 
            "givenName": "Mark A.", 
            "id": "sg:person.01106275701.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106275701.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA", 
              "id": "http://www.grid.ac/institutes/grid.240614.5", 
              "name": [
                "Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA", 
                "Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Jong", 
            "givenName": "Pieter J.", 
            "id": "sg:person.014644352072.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014644352072.19"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/359380a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050152276", 
              "https://doi.org/10.1038/359380a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/355548a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039513377", 
              "https://doi.org/10.1038/355548a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00350620", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034577152", 
              "https://doi.org/10.1007/bf00350620"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0990-841", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003598008", 
              "https://doi.org/10.1038/nbt0990-841"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0792-273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016818887", 
              "https://doi.org/10.1038/ng0792-273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/362258a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047439824", 
              "https://doi.org/10.1038/362258a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0592-132", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049337325", 
              "https://doi.org/10.1038/ng0592-132"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng0692-180", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025548112", 
              "https://doi.org/10.1038/ng0692-180"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-01", 
        "datePublishedReg": "1994-01-01", 
        "description": "We have designed a P1 vector (pCYPAC\u20131) for the introduction of recombinant DNA into E. coli using electroporation procedures. The new cloning system, P1\u2013derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130\u2013150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/ng0194-84", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1103138", 
            "issn": [
              "1061-4036", 
              "1546-1718"
            ], 
            "name": "Nature Genetics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "6"
          }
        ], 
        "keywords": [
          "cloning system", 
          "average insert size", 
          "human DNA fragments", 
          "complex genomes", 
          "clone libraries", 
          "P1 vectors", 
          "kilobase pairs", 
          "insert size", 
          "DNA fragments", 
          "recombinant DNA", 
          "new cloning system", 
          "E. coli", 
          "situ hybridization", 
          "electroporation procedure", 
          "insert instability", 
          "clones", 
          "extended culturing", 
          "genome", 
          "chromosomes", 
          "DNA", 
          "coli", 
          "hybridization", 
          "fragments", 
          "vector", 
          "culturing", 
          "chimaerism", 
          "fluorescence", 
          "library", 
          "detailed analysis", 
          "mapping", 
          "pairs", 
          "analysis", 
          "size", 
          "system", 
          "introduction", 
          "instability", 
          "propagation", 
          "procedure"
        ], 
        "name": "A new bacteriophage P1\u2013derived vector for the propagation of large human DNA fragments", 
        "pagination": "84-89", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1027542612"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ng0194-84"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8136839"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ng0194-84", 
          "https://app.dimensions.ai/details/publication/pub.1027542612"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:20", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_241.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/ng0194-84"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng0194-84'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng0194-84'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng0194-84'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng0194-84'


     

    This table displays all metadata directly associated to this object as RDF triples.

    239 TRIPLES      21 PREDICATES      82 URIs      66 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ng0194-84 schema:about N22f4a566d2294686add135a0a6f75ed4
    2 N6e84c7da73ba467ba2b537121ca83816
    3 N7623456f3f8b4524adc7c9fb4ae815f0
    4 N7d16caf51c364d548277ac69c322a573
    5 N8a35ef84e9584258851e24be4307f818
    6 Naaf1c2b8ffa94d5bb5a11a217b6755e4
    7 Nb72ed40526864e05a3cf815f63822fa4
    8 Nbde9fc78938e480cafe5247200210a48
    9 Nc64be334bb3b4cf489786f8dbd121f57
    10 Nddf0193c173249a68a987a18e63f6c9e
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author N5ee0bfeec3634485b9a6d0e4443d75d6
    14 schema:citation sg:pub.10.1007/bf00350620
    15 sg:pub.10.1038/355548a0
    16 sg:pub.10.1038/359380a0
    17 sg:pub.10.1038/362258a0
    18 sg:pub.10.1038/nbt0990-841
    19 sg:pub.10.1038/ng0592-132
    20 sg:pub.10.1038/ng0692-180
    21 sg:pub.10.1038/ng0792-273
    22 schema:datePublished 1994-01
    23 schema:datePublishedReg 1994-01-01
    24 schema:description We have designed a P1 vector (pCYPAC–1) for the introduction of recombinant DNA into E. coli using electroporation procedures. The new cloning system, P1–derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130–150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes.
    25 schema:genre article
    26 schema:isAccessibleForFree false
    27 schema:isPartOf N0d44d155610b4cee888688a4d1d196c2
    28 N99cfca565a9f4435a4513d203b9e87e8
    29 sg:journal.1103138
    30 schema:keywords DNA
    31 DNA fragments
    32 E. coli
    33 P1 vectors
    34 analysis
    35 average insert size
    36 chimaerism
    37 chromosomes
    38 clone libraries
    39 clones
    40 cloning system
    41 coli
    42 complex genomes
    43 culturing
    44 detailed analysis
    45 electroporation procedure
    46 extended culturing
    47 fluorescence
    48 fragments
    49 genome
    50 human DNA fragments
    51 hybridization
    52 insert instability
    53 insert size
    54 instability
    55 introduction
    56 kilobase pairs
    57 library
    58 mapping
    59 new cloning system
    60 pairs
    61 procedure
    62 propagation
    63 recombinant DNA
    64 situ hybridization
    65 size
    66 system
    67 vector
    68 schema:name A new bacteriophage P1–derived vector for the propagation of large human DNA fragments
    69 schema:pagination 84-89
    70 schema:productId N7fecb4692b6945eb8f23fdb702e52654
    71 N97654a0ce3ea4bb99bc51606d5e2a05b
    72 Nd7412c64bd734243a1dd50babd9dba9d
    73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027542612
    74 https://doi.org/10.1038/ng0194-84
    75 schema:sdDatePublished 2022-12-01T06:20
    76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    77 schema:sdPublisher N9fd3fdaa2ede4c77a2c3c8b07347e73f
    78 schema:url https://doi.org/10.1038/ng0194-84
    79 sgo:license sg:explorer/license/
    80 sgo:sdDataset articles
    81 rdf:type schema:ScholarlyArticle
    82 N0d44d155610b4cee888688a4d1d196c2 schema:issueNumber 1
    83 rdf:type schema:PublicationIssue
    84 N22f4a566d2294686add135a0a6f75ed4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Gene Library
    86 rdf:type schema:DefinedTerm
    87 N55b54b4bc4c34b81a1340a7cb6fe08c8 rdf:first sg:person.014644352072.19
    88 rdf:rest rdf:nil
    89 N594ac465db7942ac96143bebf0480d5d rdf:first sg:person.01232642324.09
    90 rdf:rest N747eb89eb1ea4ad084fed9304f3d9605
    91 N5ee0bfeec3634485b9a6d0e4443d75d6 rdf:first sg:person.013566425337.50
    92 rdf:rest Nf4dcf55fdb0345c9a101cf9f923f3c8e
    93 N6e84c7da73ba467ba2b537121ca83816 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Bacteriophage P1
    95 rdf:type schema:DefinedTerm
    96 N747eb89eb1ea4ad084fed9304f3d9605 rdf:first sg:person.01106275701.28
    97 rdf:rest N55b54b4bc4c34b81a1340a7cb6fe08c8
    98 N7623456f3f8b4524adc7c9fb4ae815f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name In Situ Hybridization, Fluorescence
    100 rdf:type schema:DefinedTerm
    101 N7d16caf51c364d548277ac69c322a573 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Base Sequence
    103 rdf:type schema:DefinedTerm
    104 N7fecb4692b6945eb8f23fdb702e52654 schema:name doi
    105 schema:value 10.1038/ng0194-84
    106 rdf:type schema:PropertyValue
    107 N8a35ef84e9584258851e24be4307f818 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Molecular Sequence Data
    109 rdf:type schema:DefinedTerm
    110 N97654a0ce3ea4bb99bc51606d5e2a05b schema:name dimensions_id
    111 schema:value pub.1027542612
    112 rdf:type schema:PropertyValue
    113 N99cfca565a9f4435a4513d203b9e87e8 schema:volumeNumber 6
    114 rdf:type schema:PublicationVolume
    115 N9fd3fdaa2ede4c77a2c3c8b07347e73f schema:name Springer Nature - SN SciGraph project
    116 rdf:type schema:Organization
    117 Na216f05a6acf47ccb12d01f943652b08 rdf:first sg:person.01233556444.33
    118 rdf:rest N594ac465db7942ac96143bebf0480d5d
    119 Na5efd319fbd945538b73bee5a44fd394 rdf:first sg:person.0632331622.29
    120 rdf:rest Na216f05a6acf47ccb12d01f943652b08
    121 Naaf1c2b8ffa94d5bb5a11a217b6755e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Cloning, Molecular
    123 rdf:type schema:DefinedTerm
    124 Nb72ed40526864e05a3cf815f63822fa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Escherichia coli
    126 rdf:type schema:DefinedTerm
    127 Nbde9fc78938e480cafe5247200210a48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Humans
    129 rdf:type schema:DefinedTerm
    130 Nc64be334bb3b4cf489786f8dbd121f57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name DNA, Recombinant
    132 rdf:type schema:DefinedTerm
    133 Nd7412c64bd734243a1dd50babd9dba9d schema:name pubmed_id
    134 schema:value 8136839
    135 rdf:type schema:PropertyValue
    136 Nddf0193c173249a68a987a18e63f6c9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Genetic Vectors
    138 rdf:type schema:DefinedTerm
    139 Ne18a1925944d4dd4bacdf29a7722e531 rdf:first sg:person.01167721010.47
    140 rdf:rest Na5efd319fbd945538b73bee5a44fd394
    141 Nf4dcf55fdb0345c9a101cf9f923f3c8e rdf:first sg:person.01132314305.16
    142 rdf:rest Ne18a1925944d4dd4bacdf29a7722e531
    143 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    144 schema:name Biological Sciences
    145 rdf:type schema:DefinedTerm
    146 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    147 schema:name Genetics
    148 rdf:type schema:DefinedTerm
    149 sg:journal.1103138 schema:issn 1061-4036
    150 1546-1718
    151 schema:name Nature Genetics
    152 schema:publisher Springer Nature
    153 rdf:type schema:Periodical
    154 sg:person.01106275701.28 schema:affiliation grid-institutes:grid.250008.f
    155 schema:familyName Batzer
    156 schema:givenName Mark A.
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106275701.28
    158 rdf:type schema:Person
    159 sg:person.01132314305.16 schema:affiliation grid-institutes:grid.189504.1
    160 schema:familyName Amemiya
    161 schema:givenName Chris T.
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132314305.16
    163 rdf:type schema:Person
    164 sg:person.01167721010.47 schema:affiliation grid-institutes:grid.250008.f
    165 schema:familyName Garnes
    166 schema:givenName Jeffrey
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167721010.47
    168 rdf:type schema:Person
    169 sg:person.01232642324.09 schema:affiliation grid-institutes:grid.240614.5
    170 schema:familyName Chen
    171 schema:givenName Chira
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232642324.09
    173 rdf:type schema:Person
    174 sg:person.01233556444.33 schema:affiliation grid-institutes:grid.20861.3d
    175 schema:familyName Shizuya
    176 schema:givenName Hiroaki
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233556444.33
    178 rdf:type schema:Person
    179 sg:person.013566425337.50 schema:affiliation grid-institutes:grid.417705.0
    180 schema:familyName loannou
    181 schema:givenName Panayiotis A.
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013566425337.50
    183 rdf:type schema:Person
    184 sg:person.014644352072.19 schema:affiliation grid-institutes:grid.240614.5
    185 schema:familyName de Jong
    186 schema:givenName Pieter J.
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014644352072.19
    188 rdf:type schema:Person
    189 sg:person.0632331622.29 schema:affiliation grid-institutes:None
    190 schema:familyName Kroisel
    191 schema:givenName Peter M.
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632331622.29
    193 rdf:type schema:Person
    194 sg:pub.10.1007/bf00350620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034577152
    195 https://doi.org/10.1007/bf00350620
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1038/355548a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039513377
    198 https://doi.org/10.1038/355548a0
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1038/359380a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050152276
    201 https://doi.org/10.1038/359380a0
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1038/362258a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047439824
    204 https://doi.org/10.1038/362258a0
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1038/nbt0990-841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003598008
    207 https://doi.org/10.1038/nbt0990-841
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1038/ng0592-132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049337325
    210 https://doi.org/10.1038/ng0592-132
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1038/ng0692-180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025548112
    213 https://doi.org/10.1038/ng0692-180
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1038/ng0792-273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016818887
    216 https://doi.org/10.1038/ng0792-273
    217 rdf:type schema:CreativeWork
    218 grid-institutes:None schema:alternateName Department of Medical Biology and Human Genetics, Harrachgasse 21/8, A-8010, Graz, Austria
    219 schema:name Department of Medical Biology and Human Genetics, Harrachgasse 21/8, A-8010, Graz, Austria
    220 Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    221 rdf:type schema:Organization
    222 grid-institutes:grid.189504.1 schema:alternateName Center for Human Genetics, Boston University School of Medicine, 80 East Concord St, 02118, Boston, Massachusetts, USA
    223 schema:name Center for Human Genetics, Boston University School of Medicine, 80 East Concord St, 02118, Boston, Massachusetts, USA
    224 Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    225 rdf:type schema:Organization
    226 grid-institutes:grid.20861.3d schema:alternateName Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, California, USA
    227 schema:name Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, California, USA
    228 rdf:type schema:Organization
    229 grid-institutes:grid.240614.5 schema:alternateName Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA
    230 schema:name Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, 14263, Buffalo, New York, USA
    231 Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    232 rdf:type schema:Organization
    233 grid-institutes:grid.250008.f schema:alternateName Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    234 schema:name Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    235 rdf:type schema:Organization
    236 grid-institutes:grid.417705.0 schema:alternateName The Cyprus Institute of Neurology and Genetics, P.O. Box 3462, Nicosia, Cyprus
    237 schema:name Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 94551, Livermore, California, USA
    238 The Cyprus Institute of Neurology and Genetics, P.O. Box 3462, Nicosia, Cyprus
    239 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...