A phenylalanine in DGAT is a key determinant of oil content and composition in maize View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-02-17

AUTHORS

Peizhong Zheng, William B Allen, Keith Roesler, Mark E Williams, Shirong Zhang, Jiming Li, Kimberly Glassman, Jerry Ranch, Douglas Nubel, William Solawetz, Dinakar Bhattramakki, Victor Llaca, Stéphane Deschamps, Gan-Yuan Zhong, Mitchell C Tarczynski, Bo Shen

ABSTRACT

Plant oil is an important renewable resource for biodiesel production and for dietary consumption by humans and livestock. Through genetic mapping of the oil trait in plants, studies have reported multiple quantitative trait loci (QTLs) with small effects, but the molecular basis of oil QTLs remains largely unknown1,2,3,4,5. Here we show that a high-oil QTL (qHO6) affecting maize seed oil and oleic-acid contents encodes an acyl-CoA:diacylglycerol acyltransferase (DGAT1-2), which catalyzes the final step of oil synthesis. We further show that a phenylalanine insertion in DGAT1-2 at position 469 (F469) is responsible for the increased oil and oleic-acid contents. The DGAT1-2 allele with F469 is ancestral, whereas the allele without F469 is a more recent mutant selected by domestication or breeding. Ectopic expression of the high-oil DGAT1-2 allele increases oil and oleic-acid contents by up to 41% and 107%, respectively. This work provides insights into the molecular basis of natural variation of oil and oleic-acid contents in plants and highlights DGAT as a promising target for increasing oil and oleic-acid contents in other crops. More... »

PAGES

367-372

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng.85

DOI

http://dx.doi.org/10.1038/ng.85

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030608077

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18278045


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26 schema:description Plant oil is an important renewable resource for biodiesel production and for dietary consumption by humans and livestock. Through genetic mapping of the oil trait in plants, studies have reported multiple quantitative trait loci (QTLs) with small effects, but the molecular basis of oil QTLs remains largely unknown1,2,3,4,5. Here we show that a high-oil QTL (qHO6) affecting maize seed oil and oleic-acid contents encodes an acyl-CoA:diacylglycerol acyltransferase (DGAT1-2), which catalyzes the final step of oil synthesis. We further show that a phenylalanine insertion in DGAT1-2 at position 469 (F469) is responsible for the increased oil and oleic-acid contents. The DGAT1-2 allele with F469 is ancestral, whereas the allele without F469 is a more recent mutant selected by domestication or breeding. Ectopic expression of the high-oil DGAT1-2 allele increases oil and oleic-acid contents by up to 41% and 107%, respectively. This work provides insights into the molecular basis of natural variation of oil and oleic-acid contents in plants and highlights DGAT as a promising target for increasing oil and oleic-acid contents in other crops.
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33 acyltransferase
34 alleles
35 basis
36 biodiesel production
37 breeding
38 composition
39 consumption
40 content
41 crops
42 determinants
43 diacylglycerol acyltransferase
44 dietary consumption
45 domestication
46 ectopic expression
47 effect
48 expression
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50 genetic mapping
51 humans
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53 insertion
54 insights
55 key determinant
56 livestock
57 loci
58 maize
59 mapping
60 molecular basis
61 multiple quantitative trait loci
62 mutants
63 natural variation
64 oil
65 oil content
66 oil quantitative trait loci
67 oil synthesis
68 oil traits
69 oleic acid content
70 phenylalanine
71 plant oils
72 plants
73 position 469
74 production
75 promising target
76 quantitative trait loci
77 recent mutant
78 renewable resources
79 resources
80 seed oil
81 small effect
82 step
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86 trait loci
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