Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05-03

AUTHORS

Lajos Mátés, Marinee K L Chuah, Eyayu Belay, Boris Jerchow, Namitha Manoj, Abel Acosta-Sanchez, Dawid P Grzela, Andrea Schmitt, Katja Becker, Janka Matrai, Ling Ma, Ermira Samara-Kuko, Conny Gysemans, Diana Pryputniewicz, Csaba Miskey, Bradley Fletcher, Thierry VandenDriessche, Zoltán Ivics, Zsuzsanna Izsvák

ABSTRACT

Zsuzsanna Izsvák and colleagues report the generation of a hyperactive version of the Sleeping Beauty transposase that supports efficient and stable gene transfer into human CD34+ cells enriched for hematopoietic stem or progenitor cells.

PAGES

753-761

Journal

TITLE

Nature Genetics

ISSUE

6

VOLUME

41

Related Patents

  • Enhanced Sleeping Beauty Transposons, Kits And Methods Of Transposition
  • Improved Transposon System For Gene Delivery
  • Cell Penetrating Transposase
  • Artificial Recombinant Chromosome And Use Thereof
  • Vector For Liver-Directed Gene Therapy Of Hemophilia And Methods And Use Thereof
  • Liver-Specific Nucleic Acid Regulatory Elements And Methods And Use Thereof
  • Transgene Genetic Tags And Methods Of Use
  • Transposase Polypeptides And Uses Thereof
  • Method And Compositions For Cellular Immunotherapy
  • Transposon Mutagenesis System And Methods Of Use
  • New Tools For Improving Gene Therapy And Use Thereof
  • Receptors Providing Targeted Costimulation For Adoptive Cell Therapy
  • Method For Producing Hyperactive Transposase Mutants
  • A Transposon-Based Transfection System For Primary Cells
  • Vectors For Liver-Directed Gene Therapy Of Hemophilia And Methods And Use Thereof
  • Method For Cultivating Stem Cells In Vitro
  • A Method For High Level And Stable Gene Transfer In Lymphocytes
  • Genetically Modified Rat Models For Severe Combined Immunodeficiency (Scid)
  • Genetically Modified Rat Models For Severe Combined Immunodeficiency (Scid)
  • Endogenous Retrovirus Transcription As A Marker For Primate Naïve Pluripotent Stem Cells
  • Human Application Of Engineered Chimeric Antigen Receptor (Car) T-Cells
  • Transposase Polypeptides And Uses Thereof
  • Enhanced Sleeping Beauty Transposons, Kits And Methods Of Transposition
  • Improved Transposon System For Gene Delivery
  • Anti-Cd19 Scfv (Fmc63) Polypeptide
  • Transposition-Mediated Identification Of Specific Binding Or Functional Proteins
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ng.343

    DOI

    http://dx.doi.org/10.1038/ng.343

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034767313

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19412179


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Conserved Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Transposable Elements", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Evolution, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Transgenic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Alignment", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Homology, Amino Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transposases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vertebrates", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "M\u00e1t\u00e9s", 
            "givenName": "Lajos", 
            "id": "sg:person.01204472073.46", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204472073.46"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chuah", 
            "givenName": "Marinee K L", 
            "id": "sg:person.015310703510.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015310703510.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Belay", 
            "givenName": "Eyayu", 
            "id": "sg:person.01221510522.11", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221510522.11"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jerchow", 
            "givenName": "Boris", 
            "id": "sg:person.01366044360.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366044360.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Manoj", 
            "givenName": "Namitha", 
            "id": "sg:person.014372351251.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014372351251.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Acosta-Sanchez", 
            "givenName": "Abel", 
            "id": "sg:person.01136041661.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136041661.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Grzela", 
            "givenName": "Dawid P", 
            "id": "sg:person.01215314647.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215314647.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schmitt", 
            "givenName": "Andrea", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Becker", 
            "givenName": "Katja", 
            "id": "sg:person.01111520270.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111520270.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Matrai", 
            "givenName": "Janka", 
            "id": "sg:person.01303301763.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303301763.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ma", 
            "givenName": "Ling", 
            "id": "sg:person.0601462502.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601462502.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Samara-Kuko", 
            "givenName": "Ermira", 
            "id": "sg:person.0647575702.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647575702.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Experimental Medicine, Laboratory for Experimental Transplantation, University of Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Department of Experimental Medicine, Laboratory for Experimental Transplantation, University of Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gysemans", 
            "givenName": "Conny", 
            "id": "sg:person.01160740403.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160740403.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pryputniewicz", 
            "givenName": "Diana", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Miskey", 
            "givenName": "Csaba", 
            "id": "sg:person.01057433000.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057433000.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, Florida, USA", 
              "id": "http://www.grid.ac/institutes/grid.15276.37", 
              "name": [
                "Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, Florida, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fletcher", 
            "givenName": "Bradley", 
            "id": "sg:person.07567263522.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07567263522.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium", 
              "id": "http://www.grid.ac/institutes/grid.5596.f", 
              "name": [
                "Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium"
              ], 
              "type": "Organization"
            }, 
            "familyName": "VandenDriessche", 
            "givenName": "Thierry", 
            "id": "sg:person.0771564672.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771564672.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419491.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ivics", 
            "givenName": "Zolt\u00e1n", 
            "id": "sg:person.0665574302.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665574302.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.481814.0", 
              "name": [
                "Max Delbr\u00fcck Center for Molecular Medicine, Berlin, Germany", 
                "Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Izsv\u00e1k", 
            "givenName": "Zsuzsanna", 
            "id": "sg:person.01125546200.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125546200.29"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00335-007-9025-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016451068", 
              "https://doi.org/10.1007/s00335-007-9025-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029427483", 
              "https://doi.org/10.1038/nmeth1002"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2007-8-s1-s1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028761293", 
              "https://doi.org/10.1186/gb-2007-8-s1-s1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/75568", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039686973", 
              "https://doi.org/10.1038/75568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1263", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001142224", 
              "https://doi.org/10.1038/nrmicro1263"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/76095", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021315149", 
              "https://doi.org/10.1038/76095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1358", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012134968", 
              "https://doi.org/10.1038/nm1358"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-05-03", 
        "datePublishedReg": "2009-05-03", 
        "description": "Zsuzsanna Izsv\u00e1k and colleagues report the generation of a hyperactive version of the Sleeping Beauty transposase that supports efficient and stable gene transfer into human CD34+ cells enriched for hematopoietic stem or progenitor cells.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/ng.343", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1103138", 
            "issn": [
              "1061-4036", 
              "1546-1718"
            ], 
            "name": "Nature Genetics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "41"
          }
        ], 
        "keywords": [
          "Sleeping Beauty transposase", 
          "stable gene transfer", 
          "Beauty transposase", 
          "gene transfer", 
          "hyperactive Sleeping Beauty transposase", 
          "molecular evolution", 
          "hyperactive version", 
          "hematopoietic stem", 
          "human CD34", 
          "progenitor cells", 
          "transposase", 
          "vertebrates", 
          "cells", 
          "stem", 
          "evolution", 
          "CD34", 
          "transfer", 
          "generation", 
          "colleagues", 
          "version", 
          "Zsuzsanna Izsv\u00e1k", 
          "Izsv\u00e1k", 
          "novel hyperactive Sleeping Beauty transposase", 
          "robust stable gene transfer"
        ], 
        "name": "Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates", 
        "pagination": "753-761", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1034767313"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ng.343"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19412179"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ng.343", 
          "https://app.dimensions.ai/details/publication/pub.1034767313"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-12-01T19:22", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_498.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/ng.343"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ng.343'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ng.343'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ng.343'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ng.343'


     

    This table displays all metadata directly associated to this object as RDF triples.

    306 TRIPLES      22 PREDICATES      71 URIs      56 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ng.343 schema:about N07c4cbda89ff4eaa833f14cf8a51946e
    2 N0fa7b21ec635448298e6c9411cb1b35d
    3 N34b61e826c644b8f9e04e165d6cdb931
    4 N3e288c3149b1475e954684a076def2ce
    5 N5885abe7ae8240af9c4651fe69e94eda
    6 N6ad1f4dd7e6a401bbbc3d2dca2fa2b9f
    7 N8239b058389440fba140bedc3687168c
    8 N824d4218d8904a1c835401ff89b4ac75
    9 N8624f5460cb04170aeddc33dc916f733
    10 Na44e84d383cc47e9bc141722997440a4
    11 Nad180c0304644c2bb3ccfe3a24e91f7a
    12 Nbe4ec3ba233a432aadd2223d715956f6
    13 Nd1d2feac5d6e46e78593393bebfd710a
    14 Ne85ac13759b04b92aca1becb4893f61c
    15 anzsrc-for:06
    16 anzsrc-for:0604
    17 schema:author N101a6e0d1e804f15b7f5c60ef54689d7
    18 schema:citation sg:pub.10.1007/s00335-007-9025-5
    19 sg:pub.10.1038/75568
    20 sg:pub.10.1038/76095
    21 sg:pub.10.1038/nm1358
    22 sg:pub.10.1038/nmeth1002
    23 sg:pub.10.1038/nrmicro1263
    24 sg:pub.10.1186/gb-2007-8-s1-s1
    25 schema:datePublished 2009-05-03
    26 schema:datePublishedReg 2009-05-03
    27 schema:description Zsuzsanna Izsvák and colleagues report the generation of a hyperactive version of the Sleeping Beauty transposase that supports efficient and stable gene transfer into human CD34+ cells enriched for hematopoietic stem or progenitor cells.
    28 schema:genre article
    29 schema:inLanguage en
    30 schema:isAccessibleForFree false
    31 schema:isPartOf N5807f91184704126b1f1e27b46b85c2c
    32 N64472959690048ebb07baa4c88ecee39
    33 sg:journal.1103138
    34 schema:keywords Beauty transposase
    35 CD34
    36 Izsvák
    37 Sleeping Beauty transposase
    38 Zsuzsanna Izsvák
    39 cells
    40 colleagues
    41 evolution
    42 gene transfer
    43 generation
    44 hematopoietic stem
    45 human CD34
    46 hyperactive Sleeping Beauty transposase
    47 hyperactive version
    48 molecular evolution
    49 novel hyperactive Sleeping Beauty transposase
    50 progenitor cells
    51 robust stable gene transfer
    52 stable gene transfer
    53 stem
    54 transfer
    55 transposase
    56 version
    57 vertebrates
    58 schema:name Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    59 schema:pagination 753-761
    60 schema:productId N46e099c0387a410bb719db1ac00ab633
    61 Nd316e5fb464441d4a99916c0e1d269e7
    62 Nd6caa64c432649d48246740930accbb8
    63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034767313
    64 https://doi.org/10.1038/ng.343
    65 schema:sdDatePublished 2021-12-01T19:22
    66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    67 schema:sdPublisher N3266fb3437204f3a9846266924c2a035
    68 schema:url https://doi.org/10.1038/ng.343
    69 sgo:license sg:explorer/license/
    70 sgo:sdDataset articles
    71 rdf:type schema:ScholarlyArticle
    72 N0545c4dd79a74db793525dd66cde443b rdf:first sg:person.01215314647.89
    73 rdf:rest N7d2268c2c4fb4cb49b25be789b73ac16
    74 N07c4cbda89ff4eaa833f14cf8a51946e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name Conserved Sequence
    76 rdf:type schema:DefinedTerm
    77 N0fa7b21ec635448298e6c9411cb1b35d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Molecular Sequence Data
    79 rdf:type schema:DefinedTerm
    80 N101a6e0d1e804f15b7f5c60ef54689d7 rdf:first sg:person.01204472073.46
    81 rdf:rest N85efe09191ab4f1fad9e6fe105439ced
    82 N11202aa8ebb24be2bc3672888cfd6795 rdf:first sg:person.01221510522.11
    83 rdf:rest N8a3b670de0ae40c0a6cfa5e5e0a7dfd8
    84 N168500ae1d5b4f67bbd84d70eb48ff02 rdf:first sg:person.01057433000.41
    85 rdf:rest Ne9673eb3eaa94f0b9ea4434817696dac
    86 N25dc920fb7e2448fa3509f301affe39e rdf:first sg:person.0601462502.50
    87 rdf:rest Na8c6e9aa76314876bd60508b5beb8ada
    88 N3266fb3437204f3a9846266924c2a035 schema:name Springer Nature - SN SciGraph project
    89 rdf:type schema:Organization
    90 N34b61e826c644b8f9e04e165d6cdb931 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Animals
    92 rdf:type schema:DefinedTerm
    93 N36f4b83b59154903bdacc09a68343f29 rdf:first sg:person.0771564672.10
    94 rdf:rest Nf4c057bba6774ffc925fd361c1be1819
    95 N3e288c3149b1475e954684a076def2ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Mice
    97 rdf:type schema:DefinedTerm
    98 N46e099c0387a410bb719db1ac00ab633 schema:name dimensions_id
    99 schema:value pub.1034767313
    100 rdf:type schema:PropertyValue
    101 N5807f91184704126b1f1e27b46b85c2c schema:issueNumber 6
    102 rdf:type schema:PublicationIssue
    103 N5885abe7ae8240af9c4651fe69e94eda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Sequence Alignment
    105 rdf:type schema:DefinedTerm
    106 N64472959690048ebb07baa4c88ecee39 schema:volumeNumber 41
    107 rdf:type schema:PublicationVolume
    108 N6ad1f4dd7e6a401bbbc3d2dca2fa2b9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Vertebrates
    110 rdf:type schema:DefinedTerm
    111 N7d2268c2c4fb4cb49b25be789b73ac16 rdf:first N80e0c9c7213d4b95ae1081ded0f5001e
    112 rdf:rest Nbcf4f0d3239b4ea0afbfa72a2cc5b39b
    113 N80e0c9c7213d4b95ae1081ded0f5001e schema:affiliation grid-institutes:grid.419491.0
    114 schema:familyName Schmitt
    115 schema:givenName Andrea
    116 rdf:type schema:Person
    117 N8239b058389440fba140bedc3687168c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Transposases
    119 rdf:type schema:DefinedTerm
    120 N824d4218d8904a1c835401ff89b4ac75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Sequence Homology, Amino Acid
    122 rdf:type schema:DefinedTerm
    123 N85efe09191ab4f1fad9e6fe105439ced rdf:first sg:person.015310703510.56
    124 rdf:rest N11202aa8ebb24be2bc3672888cfd6795
    125 N8624f5460cb04170aeddc33dc916f733 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Amino Acid Sequence
    127 rdf:type schema:DefinedTerm
    128 N88d45f1f7b824c8cacb112e18518182b rdf:first Ne00c0de285384b3b8297524027ef48ac
    129 rdf:rest N168500ae1d5b4f67bbd84d70eb48ff02
    130 N8a3b670de0ae40c0a6cfa5e5e0a7dfd8 rdf:first sg:person.01366044360.38
    131 rdf:rest Nb40d2a3a003149a68f145c794c57f441
    132 N907a799bb1ce4194895870ab202e5688 rdf:first sg:person.01125546200.29
    133 rdf:rest rdf:nil
    134 N93ac492a4d464a239313ad5c3221f4b1 rdf:first sg:person.01160740403.32
    135 rdf:rest N88d45f1f7b824c8cacb112e18518182b
    136 Na44e84d383cc47e9bc141722997440a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Humans
    138 rdf:type schema:DefinedTerm
    139 Na8c6e9aa76314876bd60508b5beb8ada rdf:first sg:person.0647575702.70
    140 rdf:rest N93ac492a4d464a239313ad5c3221f4b1
    141 Nad180c0304644c2bb3ccfe3a24e91f7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Evolution, Molecular
    143 rdf:type schema:DefinedTerm
    144 Nb40d2a3a003149a68f145c794c57f441 rdf:first sg:person.014372351251.26
    145 rdf:rest Nb9a2b1676c044f0c852378199c00ee1b
    146 Nb9a2b1676c044f0c852378199c00ee1b rdf:first sg:person.01136041661.62
    147 rdf:rest N0545c4dd79a74db793525dd66cde443b
    148 Nbcf4f0d3239b4ea0afbfa72a2cc5b39b rdf:first sg:person.01111520270.85
    149 rdf:rest Nf147d61f6ecd472cafca493a38d64afd
    150 Nbe4ec3ba233a432aadd2223d715956f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Phylogeny
    152 rdf:type schema:DefinedTerm
    153 Nd1d2feac5d6e46e78593393bebfd710a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Mice, Transgenic
    155 rdf:type schema:DefinedTerm
    156 Nd316e5fb464441d4a99916c0e1d269e7 schema:name doi
    157 schema:value 10.1038/ng.343
    158 rdf:type schema:PropertyValue
    159 Nd6caa64c432649d48246740930accbb8 schema:name pubmed_id
    160 schema:value 19412179
    161 rdf:type schema:PropertyValue
    162 Ne00c0de285384b3b8297524027ef48ac schema:affiliation grid-institutes:grid.419491.0
    163 schema:familyName Pryputniewicz
    164 schema:givenName Diana
    165 rdf:type schema:Person
    166 Ne85ac13759b04b92aca1becb4893f61c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name DNA Transposable Elements
    168 rdf:type schema:DefinedTerm
    169 Ne9673eb3eaa94f0b9ea4434817696dac rdf:first sg:person.07567263522.26
    170 rdf:rest N36f4b83b59154903bdacc09a68343f29
    171 Nf147d61f6ecd472cafca493a38d64afd rdf:first sg:person.01303301763.62
    172 rdf:rest N25dc920fb7e2448fa3509f301affe39e
    173 Nf4c057bba6774ffc925fd361c1be1819 rdf:first sg:person.0665574302.37
    174 rdf:rest N907a799bb1ce4194895870ab202e5688
    175 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    176 schema:name Biological Sciences
    177 rdf:type schema:DefinedTerm
    178 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    179 schema:name Genetics
    180 rdf:type schema:DefinedTerm
    181 sg:journal.1103138 schema:issn 1061-4036
    182 1546-1718
    183 schema:name Nature Genetics
    184 schema:publisher Springer Nature
    185 rdf:type schema:Periodical
    186 sg:person.01057433000.41 schema:affiliation grid-institutes:grid.419491.0
    187 schema:familyName Miskey
    188 schema:givenName Csaba
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057433000.41
    190 rdf:type schema:Person
    191 sg:person.01111520270.85 schema:affiliation grid-institutes:grid.419491.0
    192 schema:familyName Becker
    193 schema:givenName Katja
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111520270.85
    195 rdf:type schema:Person
    196 sg:person.01125546200.29 schema:affiliation grid-institutes:grid.481814.0
    197 schema:familyName Izsvák
    198 schema:givenName Zsuzsanna
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125546200.29
    200 rdf:type schema:Person
    201 sg:person.01136041661.62 schema:affiliation grid-institutes:grid.5596.f
    202 schema:familyName Acosta-Sanchez
    203 schema:givenName Abel
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136041661.62
    205 rdf:type schema:Person
    206 sg:person.01160740403.32 schema:affiliation grid-institutes:grid.5596.f
    207 schema:familyName Gysemans
    208 schema:givenName Conny
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160740403.32
    210 rdf:type schema:Person
    211 sg:person.01204472073.46 schema:affiliation grid-institutes:grid.419491.0
    212 schema:familyName Mátés
    213 schema:givenName Lajos
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204472073.46
    215 rdf:type schema:Person
    216 sg:person.01215314647.89 schema:affiliation grid-institutes:grid.419491.0
    217 schema:familyName Grzela
    218 schema:givenName Dawid P
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215314647.89
    220 rdf:type schema:Person
    221 sg:person.01221510522.11 schema:affiliation grid-institutes:grid.5596.f
    222 schema:familyName Belay
    223 schema:givenName Eyayu
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221510522.11
    225 rdf:type schema:Person
    226 sg:person.01303301763.62 schema:affiliation grid-institutes:grid.5596.f
    227 schema:familyName Matrai
    228 schema:givenName Janka
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303301763.62
    230 rdf:type schema:Person
    231 sg:person.01366044360.38 schema:affiliation grid-institutes:grid.419491.0
    232 schema:familyName Jerchow
    233 schema:givenName Boris
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366044360.38
    235 rdf:type schema:Person
    236 sg:person.014372351251.26 schema:affiliation grid-institutes:grid.419491.0
    237 schema:familyName Manoj
    238 schema:givenName Namitha
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014372351251.26
    240 rdf:type schema:Person
    241 sg:person.015310703510.56 schema:affiliation grid-institutes:grid.5596.f
    242 schema:familyName Chuah
    243 schema:givenName Marinee K L
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015310703510.56
    245 rdf:type schema:Person
    246 sg:person.0601462502.50 schema:affiliation grid-institutes:grid.5596.f
    247 schema:familyName Ma
    248 schema:givenName Ling
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601462502.50
    250 rdf:type schema:Person
    251 sg:person.0647575702.70 schema:affiliation grid-institutes:grid.5596.f
    252 schema:familyName Samara-Kuko
    253 schema:givenName Ermira
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647575702.70
    255 rdf:type schema:Person
    256 sg:person.0665574302.37 schema:affiliation grid-institutes:grid.419491.0
    257 schema:familyName Ivics
    258 schema:givenName Zoltán
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665574302.37
    260 rdf:type schema:Person
    261 sg:person.07567263522.26 schema:affiliation grid-institutes:grid.15276.37
    262 schema:familyName Fletcher
    263 schema:givenName Bradley
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07567263522.26
    265 rdf:type schema:Person
    266 sg:person.0771564672.10 schema:affiliation grid-institutes:grid.5596.f
    267 schema:familyName VandenDriessche
    268 schema:givenName Thierry
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771564672.10
    270 rdf:type schema:Person
    271 sg:pub.10.1007/s00335-007-9025-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016451068
    272 https://doi.org/10.1007/s00335-007-9025-5
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/75568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039686973
    275 https://doi.org/10.1038/75568
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1038/76095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021315149
    278 https://doi.org/10.1038/76095
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1038/nm1358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012134968
    281 https://doi.org/10.1038/nm1358
    282 rdf:type schema:CreativeWork
    283 sg:pub.10.1038/nmeth1002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029427483
    284 https://doi.org/10.1038/nmeth1002
    285 rdf:type schema:CreativeWork
    286 sg:pub.10.1038/nrmicro1263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001142224
    287 https://doi.org/10.1038/nrmicro1263
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1186/gb-2007-8-s1-s1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028761293
    290 https://doi.org/10.1186/gb-2007-8-s1-s1
    291 rdf:type schema:CreativeWork
    292 grid-institutes:grid.15276.37 schema:alternateName Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, Florida, USA
    293 schema:name Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, Florida, USA
    294 rdf:type schema:Organization
    295 grid-institutes:grid.419491.0 schema:alternateName Max Delbrück Center for Molecular Medicine, Berlin, Germany
    296 schema:name Max Delbrück Center for Molecular Medicine, Berlin, Germany
    297 rdf:type schema:Organization
    298 grid-institutes:grid.481814.0 schema:alternateName Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    299 schema:name Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    300 Max Delbrück Center for Molecular Medicine, Berlin, Germany
    301 rdf:type schema:Organization
    302 grid-institutes:grid.5596.f schema:alternateName Department of Experimental Medicine, Laboratory for Experimental Transplantation, University of Leuven, Belgium
    303 Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium
    304 schema:name Department of Experimental Medicine, Laboratory for Experimental Transplantation, University of Leuven, Belgium
    305 Flanders Institute for Biotechnology (VIB), Vesalius Research Center, University of Leuven, Leuven, Belgium
    306 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...