Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-12

AUTHORS

Qun Pan, Ofer Shai, Leo J Lee, Brendan J Frey, Benjamin J Blencowe

ABSTRACT

We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in approximately 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from approximately 95% of multiexon genes undergo alternative splicing and that there are approximately 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels. More... »

PAGES

1413-1415

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ng.259

DOI

http://dx.doi.org/10.1038/ng.259

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050283464

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18978789


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