Isolation and Genetic Localization of Three ϕX174 Promoter Regions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-06

AUTHORS

CHENG-YIEN CHEN, CLYDE A. HUTCHISON, MARSHALL HALL EDGEL

ABSTRACT

SPECIFIC sequences of nucleic acids such as ribosome binding sites1 (W. Gilbert, cited in ref. 2) and portions of the promoter region3,4 have been isolated by protecting those sequences with the relevant protein or organelle and digesting away the exposed nucleic acid. We have isolated specific sequences from the promoter regions in bacteriophage ϕX174 replicative form (RF) DNA using RNA polymerase as the protecting protein. We wish to describe that isolation and the procedures used to localize these RNA-polymerase-protected sequences within the ϕX174 genome. More... »

PAGES

233-236

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/newbio243233a0

DOI

http://dx.doi.org/10.1038/newbio243233a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005173538

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4577437


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