RNA Polymerase Binding Sites of Phage fd Replicative Form DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1972-05

AUTHORS

T. OKAMOTO, M. SUGIURA, M. TAKANAMI

ABSTRACT

WHEN RNA polymerase binds to DNA templates, a fraction of DNA is protected from nucleolytic digestion. This fraction has been assumed to represent the binding sites of RNA polymerase1–6. There is, however, no direct evidence that the isolated fragments really contain specific sites at which transcription is correctly initiated. An approach to this question is to demonstrate that the specificity of transcription is still retained in the isolated fragments. RNA chains transcribed in vitro on the doubly closed replicative form DNA (RF-I DNA) of bacteriophage fd are predominantly initiated with pppAUG—, pppGUA— and pppGUU—7 Accordingly, the DNA fraction protected by RNA polymerase against the nucleolytic digestion was isolated from the RF-I DNA RNA polymerase complex, and the template activity of the isolated fragments was examined. More... »

PAGES

108-109

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/newbio237108a0

DOI

http://dx.doi.org/10.1038/newbio237108a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027759007

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4555813


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