Comparison of Mitomycin C and X-irradiation as Blocking Agents in One-way Mixed Leucocyte Cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1972-02

AUTHORS

FRITZ H., MARILYN L.

ABSTRACT

THE mixed leucocyte culture (MLC) test1,2 has been widely used as a measure of histocompatibility in man and as a model for the recognition phase of the homograft response in vitro. Two methods have been proposed to achieve unidirectional stimulation in MLC: treatment of stimulating cells with either mitomycin C3 or X-irradiation3. The mitomycin C method has the advantage of not requiring expensive X-ray equipment and being immediately available in the laboratory; the X-ray method has the advantage of not requiring washing of the cells following treatment. Although much of the quantitative MLC work for histocompatibility testing in man has been done using mitomycin C, the general impression gained from the literature as well as the studies we report here would support the use of either method. A previous study4 suggested that irradiation is superior to mitomycin C treatment for a number of reasons, but we question those conclusions. More... »

PAGES

243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/newbio235243a0

DOI

http://dx.doi.org/10.1038/newbio235243a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048993365

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4502414


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