Comparison of Mitomycin C and X-irradiation as Blocking Agents in One-way Mixed Leucocyte Cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1972-02

AUTHORS

FRITZ H., MARILYN L.

ABSTRACT

THE mixed leucocyte culture (MLC) test1,2 has been widely used as a measure of histocompatibility in man and as a model for the recognition phase of the homograft response in vitro. Two methods have been proposed to achieve unidirectional stimulation in MLC: treatment of stimulating cells with either mitomycin C3 or X-irradiation3. The mitomycin C method has the advantage of not requiring expensive X-ray equipment and being immediately available in the laboratory; the X-ray method has the advantage of not requiring washing of the cells following treatment. Although much of the quantitative MLC work for histocompatibility testing in man has been done using mitomycin C, the general impression gained from the literature as well as the studies we report here would support the use of either method. A previous study4 suggested that irradiation is superior to mitomycin C treatment for a number of reasons, but we question those conclusions. More... »

PAGES

243

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/newbio235243a0

DOI

http://dx.doi.org/10.1038/newbio235243a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048993365

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4502414


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histocompatibility Testing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitomycins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Radiation Effects", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "H.", 
        "givenName": "FRITZ", 
        "type": "Person"
      }, 
      {
        "familyName": "L.", 
        "givenName": "MARILYN", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1080/00365516809168018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008591530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.126.4.655", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009067587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-197106000-00011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024884574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-197106000-00011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024884574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/223090a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047681889", 
          "https://doi.org/10.1038/223090a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-197103000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053333457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-197103000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053333457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.143.3608.813", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062481888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076260025", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1972-02", 
    "datePublishedReg": "1972-02-01", 
    "description": "THE mixed leucocyte culture (MLC) test1,2 has been widely used as a measure of histocompatibility in man and as a model for the recognition phase of the homograft response in vitro. Two methods have been proposed to achieve unidirectional stimulation in MLC: treatment of stimulating cells with either mitomycin C3 or X-irradiation3. The mitomycin C method has the advantage of not requiring expensive X-ray equipment and being immediately available in the laboratory; the X-ray method has the advantage of not requiring washing of the cells following treatment. Although much of the quantitative MLC work for histocompatibility testing in man has been done using mitomycin C, the general impression gained from the literature as well as the studies we report here would support the use of either method. A previous study4 suggested that irradiation is superior to mitomycin C treatment for a number of reasons, but we question those conclusions.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/newbio235243a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "60", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "235"
      }
    ], 
    "name": "Comparison of Mitomycin C and X-irradiation as Blocking Agents in One-way Mixed Leucocyte Cultures", 
    "pagination": "243", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e965b39fe7267ae5a7ad96407a78d56765a818aad82dd883732ea77f419be4dd"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "4502414"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410463"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/newbio235243a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048993365"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/newbio235243a0", 
      "https://app.dimensions.ai/details/publication/pub.1048993365"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:44", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70066_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/newbio235243a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/newbio235243a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/newbio235243a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/newbio235243a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/newbio235243a0'


 

This table displays all metadata directly associated to this object as RDF triples.

114 TRIPLES      21 PREDICATES      42 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/newbio235243a0 schema:about N06266f66b9784f89b4470d89a0460146
2 N28dff781a819435bb747948b4c1d6ed6
3 N941cd999cca546be9a307ae7e12c56b9
4 N9843c94e1d034a1aa56382e6ba703d86
5 Nd359110c4d6d4cfcb09cf2dff4733f18
6 Nfb5837fbf6a4487ea5e013aac8cc6ecf
7 anzsrc-for:11
8 anzsrc-for:1103
9 schema:author Ndf14fa95f11e4e7bbcdeb1c47f302586
10 schema:citation sg:pub.10.1038/223090a0
11 https://app.dimensions.ai/details/publication/pub.1076260025
12 https://doi.org/10.1080/00365516809168018
13 https://doi.org/10.1084/jem.126.4.655
14 https://doi.org/10.1097/00007890-197103000-00005
15 https://doi.org/10.1097/00007890-197106000-00011
16 https://doi.org/10.1126/science.143.3608.813
17 schema:datePublished 1972-02
18 schema:datePublishedReg 1972-02-01
19 schema:description THE mixed leucocyte culture (MLC) test1,2 has been widely used as a measure of histocompatibility in man and as a model for the recognition phase of the homograft response in vitro. Two methods have been proposed to achieve unidirectional stimulation in MLC: treatment of stimulating cells with either mitomycin C3 or X-irradiation3. The mitomycin C method has the advantage of not requiring expensive X-ray equipment and being immediately available in the laboratory; the X-ray method has the advantage of not requiring washing of the cells following treatment. Although much of the quantitative MLC work for histocompatibility testing in man has been done using mitomycin C, the general impression gained from the literature as well as the studies we report here would support the use of either method. A previous study4 suggested that irradiation is superior to mitomycin C treatment for a number of reasons, but we question those conclusions.
20 schema:genre research_article
21 schema:inLanguage en
22 schema:isAccessibleForFree false
23 schema:isPartOf N0c34c46842ea4b74ad1eccdcb6f9f325
24 N983450254b4f460a8dac243c2975ec34
25 sg:journal.1018957
26 schema:name Comparison of Mitomycin C and X-irradiation as Blocking Agents in One-way Mixed Leucocyte Cultures
27 schema:pagination 243
28 schema:productId N15930c61207849198bb7bb35f2327334
29 N1dd0e5a915bc473295a2746140a3b89c
30 N74963445e2fe472cb955c613cf6e3594
31 Na7a139091e304366a42d68a62fdf8f91
32 Nd987d98c8926408199853d222ce3ef7a
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048993365
34 https://doi.org/10.1038/newbio235243a0
35 schema:sdDatePublished 2019-04-11T12:44
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher N28fdc8b4cd764e6f82e1de1c08fec9f7
38 schema:url https://www.nature.com/articles/newbio235243a0
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N06266f66b9784f89b4470d89a0460146 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
43 schema:name Cells, Cultured
44 rdf:type schema:DefinedTerm
45 N0c34c46842ea4b74ad1eccdcb6f9f325 schema:volumeNumber 235
46 rdf:type schema:PublicationVolume
47 N15930c61207849198bb7bb35f2327334 schema:name dimensions_id
48 schema:value pub.1048993365
49 rdf:type schema:PropertyValue
50 N1dd0e5a915bc473295a2746140a3b89c schema:name nlm_unique_id
51 schema:value 0410463
52 rdf:type schema:PropertyValue
53 N28dff781a819435bb747948b4c1d6ed6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Leukocytes
55 rdf:type schema:DefinedTerm
56 N28fdc8b4cd764e6f82e1de1c08fec9f7 schema:name Springer Nature - SN SciGraph project
57 rdf:type schema:Organization
58 N74963445e2fe472cb955c613cf6e3594 schema:name doi
59 schema:value 10.1038/newbio235243a0
60 rdf:type schema:PropertyValue
61 N90c42c1f1a0140409a0dd300d998e893 rdf:first N98268a14d13245ccafd84d29def2a819
62 rdf:rest rdf:nil
63 N941cd999cca546be9a307ae7e12c56b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Mitomycins
65 rdf:type schema:DefinedTerm
66 N98268a14d13245ccafd84d29def2a819 schema:familyName L.
67 schema:givenName MARILYN
68 rdf:type schema:Person
69 N983450254b4f460a8dac243c2975ec34 schema:issueNumber 60
70 rdf:type schema:PublicationIssue
71 N9843c94e1d034a1aa56382e6ba703d86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Histocompatibility Testing
73 rdf:type schema:DefinedTerm
74 Na7a139091e304366a42d68a62fdf8f91 schema:name readcube_id
75 schema:value e965b39fe7267ae5a7ad96407a78d56765a818aad82dd883732ea77f419be4dd
76 rdf:type schema:PropertyValue
77 Nd359110c4d6d4cfcb09cf2dff4733f18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Radiation Effects
79 rdf:type schema:DefinedTerm
80 Nd987d98c8926408199853d222ce3ef7a schema:name pubmed_id
81 schema:value 4502414
82 rdf:type schema:PropertyValue
83 Ndf14fa95f11e4e7bbcdeb1c47f302586 rdf:first Ned0c1ab5ba324bed936f2f4a7e65194e
84 rdf:rest N90c42c1f1a0140409a0dd300d998e893
85 Ned0c1ab5ba324bed936f2f4a7e65194e schema:familyName H.
86 schema:givenName FRITZ
87 rdf:type schema:Person
88 Nfb5837fbf6a4487ea5e013aac8cc6ecf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Humans
90 rdf:type schema:DefinedTerm
91 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
92 schema:name Medical and Health Sciences
93 rdf:type schema:DefinedTerm
94 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
95 schema:name Clinical Sciences
96 rdf:type schema:DefinedTerm
97 sg:journal.1018957 schema:issn 0090-0028
98 1476-4687
99 schema:name Nature
100 rdf:type schema:Periodical
101 sg:pub.10.1038/223090a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047681889
102 https://doi.org/10.1038/223090a0
103 rdf:type schema:CreativeWork
104 https://app.dimensions.ai/details/publication/pub.1076260025 schema:CreativeWork
105 https://doi.org/10.1080/00365516809168018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008591530
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1084/jem.126.4.655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009067587
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1097/00007890-197103000-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053333457
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1097/00007890-197106000-00011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024884574
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1126/science.143.3608.813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062481888
114 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...