Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Davide Normanno, Lydia Boudarène, Claire Dugast-Darzacq, Jiji Chen, Christian Richter, Florence Proux, Olivier Bénichou, Raphaël Voituriez, Xavier Darzacq, Maxime Dahan

ABSTRACT

Many cellular functions rely on DNA-binding proteins finding and associating to specific sites in the genome. Yet the mechanisms underlying the target search remain poorly understood, especially in the case of the highly organized mammalian cell nucleus. Using as a model Tet repressors (TetRs) searching for a multi-array locus, we quantitatively analyse the search process in human cells with single-molecule tracking and single-cell protein-DNA association measurements. We find that TetRs explore the nucleus and reach their target by 3D diffusion interspersed with transient interactions with non-cognate sites, consistent with the facilitated diffusion model. Remarkably, nonspecific binding times are broadly distributed, underlining a lack of clear delimitation between specific and nonspecific interactions. However, the search kinetics is not determined by diffusive transport but by the low association rate to nonspecific sites. Altogether, our results provide a comprehensive view of the recruitment dynamics of proteins at specific loci in mammalian cells. More... »

PAGES

7357

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncomms8357

    DOI

    http://dx.doi.org/10.1038/ncomms8357

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014406710

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26151127


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