An optimized optogenetic clustering tool for probing protein interaction and function View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-09-18

AUTHORS

Amir Taslimi, Justin D. Vrana, Daniel Chen, Sofya Borinskaya, Bruce J. Mayer, Matthew J. Kennedy, Chandra L. Tucker

ABSTRACT

The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an optogenetic module, allowing spatiotemporal control of cellular processes with light. Here we report the development of a new CRY2-derived optogenetic module, ‘CRY2olig’, which induces rapid, robust, and reversible protein oligomerization in response to light. Using this module, we developed a novel protein interaction assay, Light-Induced Co-clustering, that can be used to interrogate protein interaction dynamics in live cells. In addition to use probing protein interactions, CRY2olig can also be used to induce and reversibly control diverse cellular processes with spatial and temporal resolution. Here we demonstrate disrupting clathrin-mediated endocytosis and promoting Arp2/3-mediated actin polymerization with light. These new CRY2-based approaches expand the growing arsenal of optogenetic strategies to probe cellular function. More... »

PAGES

4925

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms5925

DOI

http://dx.doi.org/10.1038/ncomms5925

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020745069

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25233328


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