An assay to image neuronal microtubule dynamics in mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-09-12

AUTHORS

Tatjana Kleele, Petar Marinković, Philip R. Williams, Sina Stern, Emily E. Weigand, Peter Engerer, Ronald Naumann, Jana Hartmann, Rosa M. Karl, Frank Bradke, Derron Bishop, Jochen Herms, Arthur Konnerth, Martin Kerschensteiner, Leanne Godinho, Thomas Misgeld

ABSTRACT

Microtubule dynamics in neurons play critical roles in physiology, injury and disease and determine microtubule orientation, the cell biological correlate of neurite polarization. Several microtubule binding proteins, including end-binding protein 3 (EB3), specifically bind to the growing plus tip of microtubules. In the past, fluorescently tagged end-binding proteins have revealed microtubule dynamics in vitro and in non-mammalian model organisms. Here, we devise an imaging assay based on transgenic mice expressing yellow fluorescent protein-tagged EB3 to study microtubules in intact mammalian neurites. Our approach allows measurement of microtubule dynamics in vivo and ex vivo in peripheral nervous system and central nervous system neurites under physiological conditions and after exposure to microtubule-modifying drugs. We find an increase in dynamic microtubules after injury and in neurodegenerative disease states, before axons show morphological indications of degeneration or regrowth. Thus increased microtubule dynamics might serve as a general indicator of neurite remodelling in health and disease. More... »

PAGES

4827

References to SciGraph publications

  • 2009-10. Considering the evolution of regeneration in the central nervous system in NATURE REVIEWS NEUROSCIENCE
  • 2005-10-07. Quantitative single-cell RT-PCR and Ca2+ imaging in brain slices in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2013-02-07. Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes in NATURE PROTOCOLS
  • 2007-06-10. Imaging axonal transport of mitochondria in vivo in NATURE METHODS
  • 2002-08-12. Pre-existing pathways promote precise projection patterns in NATURE NEUROSCIENCE
  • 2011-03-27. A reversible form of axon damage in experimental autoimmune encephalomyelitis and multiple sclerosis in NATURE MEDICINE
  • 1984-11. Dynamic instability of microtubule growth in NATURE
  • 2003-04-14. AMPA receptors regulate dynamic equilibrium of presynaptic terminals in mature hippocampal networks in NATURE NEUROSCIENCE
  • 2009-09. Experience-dependent structural synaptic plasticity in the mammalian brain in NATURE REVIEWS NEUROSCIENCE
  • 2004-04. Microtubules as a target for anticancer drugs in NATURE REVIEWS CANCER
  • 2009-07-16. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window in NATURE PROTOCOLS
  • 2005-04-10. In vivo imaging of axonal degeneration and regeneration in the injured spinal cord in NATURE MEDICINE
  • 2011-07-24. HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1–induced Charcot-Marie-Tooth disease in NATURE MEDICINE
  • 2014-02-27. Acquisition frame rate affects microtubule plus-end tracking analysis in NATURE METHODS
  • 2008-09-25. Ex vivo imaging of motor axon dynamics in murine triangularis sterni explants in NATURE PROTOCOLS
  • 2009-05. Microtubule assembly, organization and dynamics in axons and dendrites in NATURE REVIEWS NEUROSCIENCE
  • Journal

    TITLE

    Nature Communications

    ISSUE

    1

    VOLUME

    5

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncomms5827

    DOI

    http://dx.doi.org/10.1038/ncomms5827

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004237926

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25219969


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Neurosciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amyotrophic Lateral Sclerosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biological Assay", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Polarity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Disease Models, Animal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ganglia, Spinal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hippocampus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Luminescent Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Transgenic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microtubule-Associated Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microtubules", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Imaging", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neurons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Primary Cell Culture", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thy-1 Antigens", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Video Recording", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kleele", 
            "givenName": "Tatjana", 
            "id": "sg:person.01246432263.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246432263.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.424247.3", 
              "name": [
                "Center for Neuropathology and Prion Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377, Munich, Germany", 
                "German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Marinkovi\u0107", 
            "givenName": "Petar", 
            "id": "sg:person.0712704250.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712704250.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Williams", 
            "givenName": "Philip R.", 
            "id": "sg:person.01134456153.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134456153.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany", 
              "id": "http://www.grid.ac/institutes/grid.424247.3", 
              "name": [
                "Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stern", 
            "givenName": "Sina", 
            "id": "sg:person.01027434031.93", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027434031.93"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Indiana University School of Medicine\u2013Muncie, Muncie, Indiana 47306, USA", 
              "id": "http://www.grid.ac/institutes/grid.257410.5", 
              "name": [
                "Department of Physiology, Indiana University School of Medicine\u2013Muncie, Muncie, Indiana 47306, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Weigand", 
            "givenName": "Emily E.", 
            "id": "sg:person.01333356700.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333356700.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Engerer", 
            "givenName": "Peter", 
            "id": "sg:person.01000555601.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000555601.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Transgenic Core Facility, Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany", 
              "id": "http://www.grid.ac/institutes/grid.419537.d", 
              "name": [
                "Transgenic Core Facility, Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Naumann", 
            "givenName": "Ronald", 
            "id": "sg:person.01361607075.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361607075.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Neuroscience, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuroscience, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hartmann", 
            "givenName": "Jana", 
            "id": "sg:person.0707225043.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707225043.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Neuroscience, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuroscience, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Karl", 
            "givenName": "Rosa M.", 
            "id": "sg:person.0735667564.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735667564.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany", 
              "id": "http://www.grid.ac/institutes/grid.424247.3", 
              "name": [
                "Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bradke", 
            "givenName": "Frank", 
            "id": "sg:person.0616321471.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616321471.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physiology, Indiana University School of Medicine\u2013Muncie, Muncie, Indiana 47306, USA", 
              "id": "http://www.grid.ac/institutes/grid.257410.5", 
              "name": [
                "Department of Physiology, Indiana University School of Medicine\u2013Muncie, Muncie, Indiana 47306, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bishop", 
            "givenName": "Derron", 
            "id": "sg:person.01156217701.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156217701.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.452617.3", 
              "name": [
                "Center for Neuropathology and Prion Research, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377, Munich, Germany", 
                "German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany", 
                "Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Herms", 
            "givenName": "Jochen", 
            "id": "sg:person.0612211244.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612211244.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.452329.b", 
              "name": [
                "Institute of Neuroscience, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
                "Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany", 
                "Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Konnerth", 
            "givenName": "Arthur", 
            "id": "sg:person.01165460062.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165460062.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Clinical Neuroimmunology, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, 81377, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.5252.0", 
              "name": [
                "Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany", 
                "Institute of Clinical Neuroimmunology, Ludwig-Maximilians Universit\u00e4t M\u00fcnchen, 81377, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kerschensteiner", 
            "givenName": "Martin", 
            "id": "sg:person.0730072347.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730072347.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.6936.a", 
              "name": [
                "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Godinho", 
            "givenName": "Leanne", 
            "id": "sg:person.01371764213.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371764213.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany", 
              "id": "http://www.grid.ac/institutes/grid.452329.b", 
              "name": [
                "Institute of Neuronal Cell Biology, Technische Universit\u00e4t M\u00fcnchen, 80802, Munich, Germany", 
                "German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany", 
                "Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany", 
                "Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Misgeld", 
            "givenName": "Thomas", 
            "id": "sg:person.01261525745.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261525745.69"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nm.2396", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015855391", 
              "https://doi.org/10.1038/nm.2396"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00424-005-1514-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000987509", 
              "https://doi.org/10.1007/s00424-005-1514-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001942463", 
              "https://doi.org/10.1038/nn1046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2846", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004797150", 
              "https://doi.org/10.1038/nmeth.2846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/312237a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034838064", 
              "https://doi.org/10.1038/312237a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrn2707", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030546428", 
              "https://doi.org/10.1038/nrn2707"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1229", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045944357", 
              "https://doi.org/10.1038/nm1229"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.2324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021474018", 
              "https://doi.org/10.1038/nm.2324"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020709483", 
              "https://doi.org/10.1038/nrc1317"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046646859", 
              "https://doi.org/10.1038/nmeth1055"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2013.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017778727", 
              "https://doi.org/10.1038/nprot.2013.022"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn905", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039599334", 
              "https://doi.org/10.1038/nn905"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrn2699", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016014531", 
              "https://doi.org/10.1038/nrn2699"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2009.89", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002599380", 
              "https://doi.org/10.1038/nprot.2009.89"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrn2631", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042050359", 
              "https://doi.org/10.1038/nrn2631"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2008.160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039491953", 
              "https://doi.org/10.1038/nprot.2008.160"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2014-09-12", 
        "datePublishedReg": "2014-09-12", 
        "description": "Microtubule dynamics in neurons play critical roles in physiology, injury and disease and determine microtubule orientation, the cell biological correlate of neurite polarization. Several microtubule binding proteins, including end-binding protein 3 (EB3), specifically bind to the growing plus tip of microtubules. In the past, fluorescently tagged end-binding proteins have revealed microtubule dynamics in vitro and in non-mammalian model organisms. Here, we devise an imaging assay based on transgenic mice expressing yellow fluorescent protein-tagged EB3 to study microtubules in intact mammalian neurites. Our approach allows measurement of microtubule dynamics in vivo and ex vivo in peripheral nervous system and central nervous system neurites under physiological conditions and after exposure to microtubule-modifying drugs. We find an increase in dynamic microtubules after injury and in neurodegenerative disease states, before axons show morphological indications of degeneration or regrowth. Thus increased microtubule dynamics might serve as a general indicator of neurite remodelling in health and disease.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/ncomms5827", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.3793702", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3128364", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3800193", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1043282", 
            "issn": [
              "2041-1723"
            ], 
            "name": "Nature Communications", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "5"
          }
        ], 
        "keywords": [
          "end-binding protein 3", 
          "microtubule dynamics", 
          "non-mammalian model organisms", 
          "end-binding proteins", 
          "neuronal microtubule dynamics", 
          "tips of microtubules", 
          "neurodegenerative disease states", 
          "model organisms", 
          "dynamic microtubules", 
          "microtubule orientation", 
          "nervous system", 
          "microtubules", 
          "peripheral nervous system", 
          "protein 3", 
          "central nervous system", 
          "physiological conditions", 
          "neurite polarization", 
          "critical role", 
          "protein", 
          "transgenic mice", 
          "morphological indications", 
          "disease states", 
          "ex vivo", 
          "biological correlates", 
          "vivo", 
          "injury", 
          "organisms", 
          "disease", 
          "mice", 
          "physiology", 
          "dynamics", 
          "vitro", 
          "assays", 
          "remodelling", 
          "neurites", 
          "axons", 
          "degeneration", 
          "neurons", 
          "general indicator", 
          "drugs", 
          "role", 
          "health", 
          "exposure", 
          "regrowth", 
          "indications", 
          "correlates", 
          "tip", 
          "increase", 
          "indicators", 
          "system", 
          "conditions", 
          "approach", 
          "state", 
          "measurements", 
          "past", 
          "orientation", 
          "polarization"
        ], 
        "name": "An assay to image neuronal microtubule dynamics in mice", 
        "pagination": "4827", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1004237926"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ncomms5827"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "25219969"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ncomms5827", 
          "https://app.dimensions.ai/details/publication/pub.1004237926"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-10-01T06:39", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_644.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/ncomms5827"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncomms5827'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncomms5827'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncomms5827'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncomms5827'


     

    This table displays all metadata directly associated to this object as RDF triples.

    422 TRIPLES      21 PREDICATES      123 URIs      97 LITERALS      30 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ncomms5827 schema:about N1a0776ae7dbd4c179bb281af81ddb518
    2 N32d40859f1fc42e38d213e89228950f7
    3 N3b1ee9af396440fa89cc192b59c919bd
    4 N4730d01b915e4b559a00f5293df46894
    5 N5023b370b9bb4c9cbe4344d02b570df4
    6 N5a3215d657c84ba5bb14ebb1bacef3c4
    7 N6ebc49ad53704601b10f95df057476e9
    8 N6fca282495b34297a92b69867c79a10a
    9 N70a60fb86fe64fcd8ca843dc2ba4e95f
    10 N7581d00ea3ac4e8fb1aef1f7f3ffbc30
    11 N76054adc2f0b45a59e20844aefd1e755
    12 N797c39f4a7a446da8a096f39daaa91d3
    13 N7a199cd25cf9490bb83f4c65345b7db4
    14 N838ea6876125449d910caef31f54c21e
    15 Nabd9dd22868c4823b2b23b26b5119eb7
    16 Nadf1a4a3b50f4a0aa70a2804e67aac1a
    17 Nae1560159b8d4874b32b526fae4a05be
    18 Nb25bd1b502fd4d529f28d3a7ba2957c0
    19 Nb4787a0713a6418ba96081322ce7d5c1
    20 Nc090ed6605ab436ab7fc8339252d0392
    21 Ndef864ef996843f3ae9c4be52a46aa59
    22 Ne127b2aafbb644d993ab5c645a6a4fb6
    23 Nf78e9a26f91f4b91b36ecfe78fbe2ef8
    24 anzsrc-for:06
    25 anzsrc-for:0601
    26 anzsrc-for:11
    27 anzsrc-for:1109
    28 schema:author N2b3035e1dbb442769be045db624ee6c3
    29 schema:citation sg:pub.10.1007/s00424-005-1514-3
    30 sg:pub.10.1038/312237a0
    31 sg:pub.10.1038/nm.2324
    32 sg:pub.10.1038/nm.2396
    33 sg:pub.10.1038/nm1229
    34 sg:pub.10.1038/nmeth.2846
    35 sg:pub.10.1038/nmeth1055
    36 sg:pub.10.1038/nn1046
    37 sg:pub.10.1038/nn905
    38 sg:pub.10.1038/nprot.2008.160
    39 sg:pub.10.1038/nprot.2009.89
    40 sg:pub.10.1038/nprot.2013.022
    41 sg:pub.10.1038/nrc1317
    42 sg:pub.10.1038/nrn2631
    43 sg:pub.10.1038/nrn2699
    44 sg:pub.10.1038/nrn2707
    45 schema:datePublished 2014-09-12
    46 schema:datePublishedReg 2014-09-12
    47 schema:description Microtubule dynamics in neurons play critical roles in physiology, injury and disease and determine microtubule orientation, the cell biological correlate of neurite polarization. Several microtubule binding proteins, including end-binding protein 3 (EB3), specifically bind to the growing plus tip of microtubules. In the past, fluorescently tagged end-binding proteins have revealed microtubule dynamics in vitro and in non-mammalian model organisms. Here, we devise an imaging assay based on transgenic mice expressing yellow fluorescent protein-tagged EB3 to study microtubules in intact mammalian neurites. Our approach allows measurement of microtubule dynamics in vivo and ex vivo in peripheral nervous system and central nervous system neurites under physiological conditions and after exposure to microtubule-modifying drugs. We find an increase in dynamic microtubules after injury and in neurodegenerative disease states, before axons show morphological indications of degeneration or regrowth. Thus increased microtubule dynamics might serve as a general indicator of neurite remodelling in health and disease.
    48 schema:genre article
    49 schema:isAccessibleForFree true
    50 schema:isPartOf N3529ef68353943d5b8c9e16e54b50c17
    51 N914d8f2cfce1491ba52b7b2b0d820ac8
    52 sg:journal.1043282
    53 schema:keywords approach
    54 assays
    55 axons
    56 biological correlates
    57 central nervous system
    58 conditions
    59 correlates
    60 critical role
    61 degeneration
    62 disease
    63 disease states
    64 drugs
    65 dynamic microtubules
    66 dynamics
    67 end-binding protein 3
    68 end-binding proteins
    69 ex vivo
    70 exposure
    71 general indicator
    72 health
    73 increase
    74 indications
    75 indicators
    76 injury
    77 measurements
    78 mice
    79 microtubule dynamics
    80 microtubule orientation
    81 microtubules
    82 model organisms
    83 morphological indications
    84 nervous system
    85 neurite polarization
    86 neurites
    87 neurodegenerative disease states
    88 neuronal microtubule dynamics
    89 neurons
    90 non-mammalian model organisms
    91 organisms
    92 orientation
    93 past
    94 peripheral nervous system
    95 physiological conditions
    96 physiology
    97 polarization
    98 protein
    99 protein 3
    100 regrowth
    101 remodelling
    102 role
    103 state
    104 system
    105 tip
    106 tips of microtubules
    107 transgenic mice
    108 vitro
    109 vivo
    110 schema:name An assay to image neuronal microtubule dynamics in mice
    111 schema:pagination 4827
    112 schema:productId N05891480f62541c0935e003075975461
    113 N31908eb546a744f6bf3d7e779d80b34e
    114 N6d46ea53a07d4fb3b1fca5fdb4f54f78
    115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004237926
    116 https://doi.org/10.1038/ncomms5827
    117 schema:sdDatePublished 2022-10-01T06:39
    118 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    119 schema:sdPublisher N52b8457a80c242ba9e37f3ab9f90fa2a
    120 schema:url https://doi.org/10.1038/ncomms5827
    121 sgo:license sg:explorer/license/
    122 sgo:sdDataset articles
    123 rdf:type schema:ScholarlyArticle
    124 N05891480f62541c0935e003075975461 schema:name pubmed_id
    125 schema:value 25219969
    126 rdf:type schema:PropertyValue
    127 N0dbf208fefda44cc8373ba7ba7926728 rdf:first sg:person.0735667564.45
    128 rdf:rest Ne2ad7b58c0db4bea8bc3a9224ef7c89d
    129 N0e961318bfa348a7a6943a4e3c16b3f4 rdf:first sg:person.01134456153.45
    130 rdf:rest Ncb3f70267ae544e2afbcf399b8ef66bc
    131 N1a0776ae7dbd4c179bb281af81ddb518 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Molecular Imaging
    133 rdf:type schema:DefinedTerm
    134 N22188565273c4909a7f6e3714277eeac rdf:first sg:person.01361607075.31
    135 rdf:rest Nbd9d2f7495e04bc9bab143d7308008de
    136 N2b3035e1dbb442769be045db624ee6c3 rdf:first sg:person.01246432263.77
    137 rdf:rest N85d9732b2e274a74824155893cf689fd
    138 N31908eb546a744f6bf3d7e779d80b34e schema:name doi
    139 schema:value 10.1038/ncomms5827
    140 rdf:type schema:PropertyValue
    141 N32d40859f1fc42e38d213e89228950f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Video Recording
    143 rdf:type schema:DefinedTerm
    144 N3529ef68353943d5b8c9e16e54b50c17 schema:volumeNumber 5
    145 rdf:type schema:PublicationVolume
    146 N3b1ee9af396440fa89cc192b59c919bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Mice, Transgenic
    148 rdf:type schema:DefinedTerm
    149 N43ac978fc09944cabdca70e2f656877f rdf:first sg:person.01371764213.22
    150 rdf:rest N6fca00a1d994407f89e15f52afab581e
    151 N4730d01b915e4b559a00f5293df46894 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Primary Cell Culture
    153 rdf:type schema:DefinedTerm
    154 N5023b370b9bb4c9cbe4344d02b570df4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Disease Models, Animal
    156 rdf:type schema:DefinedTerm
    157 N52b8457a80c242ba9e37f3ab9f90fa2a schema:name Springer Nature - SN SciGraph project
    158 rdf:type schema:Organization
    159 N5a3215d657c84ba5bb14ebb1bacef3c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Neurons
    161 rdf:type schema:DefinedTerm
    162 N6d46ea53a07d4fb3b1fca5fdb4f54f78 schema:name dimensions_id
    163 schema:value pub.1004237926
    164 rdf:type schema:PropertyValue
    165 N6ebc49ad53704601b10f95df057476e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Promoter Regions, Genetic
    167 rdf:type schema:DefinedTerm
    168 N6fca00a1d994407f89e15f52afab581e rdf:first sg:person.01261525745.69
    169 rdf:rest rdf:nil
    170 N6fca282495b34297a92b69867c79a10a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Bacterial Proteins
    172 rdf:type schema:DefinedTerm
    173 N70a60fb86fe64fcd8ca843dc2ba4e95f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Amyotrophic Lateral Sclerosis
    175 rdf:type schema:DefinedTerm
    176 N7581d00ea3ac4e8fb1aef1f7f3ffbc30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Animals
    178 rdf:type schema:DefinedTerm
    179 N76054adc2f0b45a59e20844aefd1e755 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Mice
    181 rdf:type schema:DefinedTerm
    182 N797c39f4a7a446da8a096f39daaa91d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Cell Polarity
    184 rdf:type schema:DefinedTerm
    185 N7a199cd25cf9490bb83f4c65345b7db4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    186 schema:name Thy-1 Antigens
    187 rdf:type schema:DefinedTerm
    188 N7b7e5fcf7051471783a7c66a966b08f0 rdf:first sg:person.0612211244.56
    189 rdf:rest Nc2f2ffbe36914cd187fca9ee05179003
    190 N838ea6876125449d910caef31f54c21e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    191 schema:name Ganglia, Spinal
    192 rdf:type schema:DefinedTerm
    193 N85d9732b2e274a74824155893cf689fd rdf:first sg:person.0712704250.51
    194 rdf:rest N0e961318bfa348a7a6943a4e3c16b3f4
    195 N914d8f2cfce1491ba52b7b2b0d820ac8 schema:issueNumber 1
    196 rdf:type schema:PublicationIssue
    197 Nabd9dd22868c4823b2b23b26b5119eb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Hippocampus
    199 rdf:type schema:DefinedTerm
    200 Nada65e72e5244dcfb240d7cf4a5a6d74 rdf:first sg:person.01333356700.22
    201 rdf:rest Nec6726cf46514522857446aa99df4223
    202 Nadf1a4a3b50f4a0aa70a2804e67aac1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    203 schema:name Biological Assay
    204 rdf:type schema:DefinedTerm
    205 Nae1560159b8d4874b32b526fae4a05be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Luminescent Proteins
    207 rdf:type schema:DefinedTerm
    208 Nb25bd1b502fd4d529f28d3a7ba2957c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    209 schema:name Gene Expression
    210 rdf:type schema:DefinedTerm
    211 Nb4787a0713a6418ba96081322ce7d5c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    212 schema:name Microtubule-Associated Proteins
    213 rdf:type schema:DefinedTerm
    214 Nbd9d2f7495e04bc9bab143d7308008de rdf:first sg:person.0707225043.22
    215 rdf:rest N0dbf208fefda44cc8373ba7ba7926728
    216 Nc090ed6605ab436ab7fc8339252d0392 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    217 schema:name Female
    218 rdf:type schema:DefinedTerm
    219 Nc2f2ffbe36914cd187fca9ee05179003 rdf:first sg:person.01165460062.45
    220 rdf:rest Nd154db9732e94a6984c8cd19fdd868b6
    221 Ncb3f70267ae544e2afbcf399b8ef66bc rdf:first sg:person.01027434031.93
    222 rdf:rest Nada65e72e5244dcfb240d7cf4a5a6d74
    223 Nd154db9732e94a6984c8cd19fdd868b6 rdf:first sg:person.0730072347.37
    224 rdf:rest N43ac978fc09944cabdca70e2f656877f
    225 Ndef864ef996843f3ae9c4be52a46aa59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    226 schema:name Male
    227 rdf:type schema:DefinedTerm
    228 Ne127b2aafbb644d993ab5c645a6a4fb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    229 schema:name Microtubules
    230 rdf:type schema:DefinedTerm
    231 Ne2ad7b58c0db4bea8bc3a9224ef7c89d rdf:first sg:person.0616321471.14
    232 rdf:rest Nf538b4137c2841e3a5500dc004bd76c1
    233 Nec6726cf46514522857446aa99df4223 rdf:first sg:person.01000555601.00
    234 rdf:rest N22188565273c4909a7f6e3714277eeac
    235 Nf538b4137c2841e3a5500dc004bd76c1 rdf:first sg:person.01156217701.79
    236 rdf:rest N7b7e5fcf7051471783a7c66a966b08f0
    237 Nf78e9a26f91f4b91b36ecfe78fbe2ef8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    238 schema:name Recombinant Fusion Proteins
    239 rdf:type schema:DefinedTerm
    240 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    241 schema:name Biological Sciences
    242 rdf:type schema:DefinedTerm
    243 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    244 schema:name Biochemistry and Cell Biology
    245 rdf:type schema:DefinedTerm
    246 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    247 schema:name Medical and Health Sciences
    248 rdf:type schema:DefinedTerm
    249 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
    250 schema:name Neurosciences
    251 rdf:type schema:DefinedTerm
    252 sg:grant.3128364 http://pending.schema.org/fundedItem sg:pub.10.1038/ncomms5827
    253 rdf:type schema:MonetaryGrant
    254 sg:grant.3793702 http://pending.schema.org/fundedItem sg:pub.10.1038/ncomms5827
    255 rdf:type schema:MonetaryGrant
    256 sg:grant.3800193 http://pending.schema.org/fundedItem sg:pub.10.1038/ncomms5827
    257 rdf:type schema:MonetaryGrant
    258 sg:journal.1043282 schema:issn 2041-1723
    259 schema:name Nature Communications
    260 schema:publisher Springer Nature
    261 rdf:type schema:Periodical
    262 sg:person.01000555601.00 schema:affiliation grid-institutes:grid.6936.a
    263 schema:familyName Engerer
    264 schema:givenName Peter
    265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000555601.00
    266 rdf:type schema:Person
    267 sg:person.01027434031.93 schema:affiliation grid-institutes:grid.424247.3
    268 schema:familyName Stern
    269 schema:givenName Sina
    270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01027434031.93
    271 rdf:type schema:Person
    272 sg:person.01134456153.45 schema:affiliation grid-institutes:grid.6936.a
    273 schema:familyName Williams
    274 schema:givenName Philip R.
    275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134456153.45
    276 rdf:type schema:Person
    277 sg:person.01156217701.79 schema:affiliation grid-institutes:grid.257410.5
    278 schema:familyName Bishop
    279 schema:givenName Derron
    280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156217701.79
    281 rdf:type schema:Person
    282 sg:person.01165460062.45 schema:affiliation grid-institutes:grid.452329.b
    283 schema:familyName Konnerth
    284 schema:givenName Arthur
    285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165460062.45
    286 rdf:type schema:Person
    287 sg:person.01246432263.77 schema:affiliation grid-institutes:grid.6936.a
    288 schema:familyName Kleele
    289 schema:givenName Tatjana
    290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01246432263.77
    291 rdf:type schema:Person
    292 sg:person.01261525745.69 schema:affiliation grid-institutes:grid.452329.b
    293 schema:familyName Misgeld
    294 schema:givenName Thomas
    295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261525745.69
    296 rdf:type schema:Person
    297 sg:person.01333356700.22 schema:affiliation grid-institutes:grid.257410.5
    298 schema:familyName Weigand
    299 schema:givenName Emily E.
    300 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333356700.22
    301 rdf:type schema:Person
    302 sg:person.01361607075.31 schema:affiliation grid-institutes:grid.419537.d
    303 schema:familyName Naumann
    304 schema:givenName Ronald
    305 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01361607075.31
    306 rdf:type schema:Person
    307 sg:person.01371764213.22 schema:affiliation grid-institutes:grid.6936.a
    308 schema:familyName Godinho
    309 schema:givenName Leanne
    310 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371764213.22
    311 rdf:type schema:Person
    312 sg:person.0612211244.56 schema:affiliation grid-institutes:grid.452617.3
    313 schema:familyName Herms
    314 schema:givenName Jochen
    315 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612211244.56
    316 rdf:type schema:Person
    317 sg:person.0616321471.14 schema:affiliation grid-institutes:grid.424247.3
    318 schema:familyName Bradke
    319 schema:givenName Frank
    320 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616321471.14
    321 rdf:type schema:Person
    322 sg:person.0707225043.22 schema:affiliation grid-institutes:grid.6936.a
    323 schema:familyName Hartmann
    324 schema:givenName Jana
    325 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707225043.22
    326 rdf:type schema:Person
    327 sg:person.0712704250.51 schema:affiliation grid-institutes:grid.424247.3
    328 schema:familyName Marinković
    329 schema:givenName Petar
    330 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712704250.51
    331 rdf:type schema:Person
    332 sg:person.0730072347.37 schema:affiliation grid-institutes:grid.5252.0
    333 schema:familyName Kerschensteiner
    334 schema:givenName Martin
    335 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730072347.37
    336 rdf:type schema:Person
    337 sg:person.0735667564.45 schema:affiliation grid-institutes:grid.6936.a
    338 schema:familyName Karl
    339 schema:givenName Rosa M.
    340 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735667564.45
    341 rdf:type schema:Person
    342 sg:pub.10.1007/s00424-005-1514-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000987509
    343 https://doi.org/10.1007/s00424-005-1514-3
    344 rdf:type schema:CreativeWork
    345 sg:pub.10.1038/312237a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034838064
    346 https://doi.org/10.1038/312237a0
    347 rdf:type schema:CreativeWork
    348 sg:pub.10.1038/nm.2324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021474018
    349 https://doi.org/10.1038/nm.2324
    350 rdf:type schema:CreativeWork
    351 sg:pub.10.1038/nm.2396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015855391
    352 https://doi.org/10.1038/nm.2396
    353 rdf:type schema:CreativeWork
    354 sg:pub.10.1038/nm1229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045944357
    355 https://doi.org/10.1038/nm1229
    356 rdf:type schema:CreativeWork
    357 sg:pub.10.1038/nmeth.2846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004797150
    358 https://doi.org/10.1038/nmeth.2846
    359 rdf:type schema:CreativeWork
    360 sg:pub.10.1038/nmeth1055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046646859
    361 https://doi.org/10.1038/nmeth1055
    362 rdf:type schema:CreativeWork
    363 sg:pub.10.1038/nn1046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001942463
    364 https://doi.org/10.1038/nn1046
    365 rdf:type schema:CreativeWork
    366 sg:pub.10.1038/nn905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039599334
    367 https://doi.org/10.1038/nn905
    368 rdf:type schema:CreativeWork
    369 sg:pub.10.1038/nprot.2008.160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039491953
    370 https://doi.org/10.1038/nprot.2008.160
    371 rdf:type schema:CreativeWork
    372 sg:pub.10.1038/nprot.2009.89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002599380
    373 https://doi.org/10.1038/nprot.2009.89
    374 rdf:type schema:CreativeWork
    375 sg:pub.10.1038/nprot.2013.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017778727
    376 https://doi.org/10.1038/nprot.2013.022
    377 rdf:type schema:CreativeWork
    378 sg:pub.10.1038/nrc1317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020709483
    379 https://doi.org/10.1038/nrc1317
    380 rdf:type schema:CreativeWork
    381 sg:pub.10.1038/nrn2631 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042050359
    382 https://doi.org/10.1038/nrn2631
    383 rdf:type schema:CreativeWork
    384 sg:pub.10.1038/nrn2699 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016014531
    385 https://doi.org/10.1038/nrn2699
    386 rdf:type schema:CreativeWork
    387 sg:pub.10.1038/nrn2707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030546428
    388 https://doi.org/10.1038/nrn2707
    389 rdf:type schema:CreativeWork
    390 grid-institutes:grid.257410.5 schema:alternateName Department of Physiology, Indiana University School of Medicine–Muncie, Muncie, Indiana 47306, USA
    391 schema:name Department of Physiology, Indiana University School of Medicine–Muncie, Muncie, Indiana 47306, USA
    392 rdf:type schema:Organization
    393 grid-institutes:grid.419537.d schema:alternateName Transgenic Core Facility, Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany
    394 schema:name Transgenic Core Facility, Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany
    395 rdf:type schema:Organization
    396 grid-institutes:grid.424247.3 schema:alternateName Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany
    397 German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany
    398 schema:name Axon Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), 53175, Bonn, Germany
    399 Center for Neuropathology and Prion Research, Ludwig-Maximilians-Universität München, 81377, Munich, Germany
    400 German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany
    401 rdf:type schema:Organization
    402 grid-institutes:grid.452329.b schema:alternateName Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany
    403 schema:name Center of Integrated Protein Science (CIPSM), 81377, Munich, Germany
    404 German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany
    405 Institute of Neuronal Cell Biology, Technische Universität München, 80802, Munich, Germany
    406 Institute of Neuroscience, Technische Universität München, 80802, Munich, Germany
    407 Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany
    408 rdf:type schema:Organization
    409 grid-institutes:grid.452617.3 schema:alternateName Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany
    410 schema:name Center for Neuropathology and Prion Research, Ludwig-Maximilians-Universität München, 81377, Munich, Germany
    411 German Center for Neurodegenerative Diseases (DZNE), 80336, Munich, Germany
    412 Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany
    413 rdf:type schema:Organization
    414 grid-institutes:grid.5252.0 schema:alternateName Institute of Clinical Neuroimmunology, Ludwig-Maximilians Universität München, 81377, Munich, Germany
    415 schema:name Institute of Clinical Neuroimmunology, Ludwig-Maximilians Universität München, 81377, Munich, Germany
    416 Munich Cluster for Systems Neurology (SyNergy), 80336, Munich, Germany
    417 rdf:type schema:Organization
    418 grid-institutes:grid.6936.a schema:alternateName Institute of Neuronal Cell Biology, Technische Universität München, 80802, Munich, Germany
    419 Institute of Neuroscience, Technische Universität München, 80802, Munich, Germany
    420 schema:name Institute of Neuronal Cell Biology, Technische Universität München, 80802, Munich, Germany
    421 Institute of Neuroscience, Technische Universität München, 80802, Munich, Germany
    422 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...