Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12

AUTHORS

Fayza Daboussi, Sophie Leduc, Alan Maréchal, Gwendoline Dubois, Valérie Guyot, Christophe Perez-Michaut, Alberto Amato, Angela Falciatore, Alexandre Juillerat, Marine Beurdeley, Daniel F. Voytas, Laurent Cavarec, Philippe Duchateau

ABSTRACT

Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production. More... »

PAGES

3831

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms4831

DOI

http://dx.doi.org/10.1038/ncomms4831

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004205691

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24871200


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