Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-06-28

AUTHORS

Weixin Tang, Johnny H. Hu, David R. Liu

ABSTRACT

Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly advanced our ability to manipulate the human genome. Although canonical forms of genome-editing agents and programmable transcriptional regulators are constitutively active, precise temporal and spatial control over genome editing and transcriptional regulation activities would enable the more selective and potentially safer use of these powerful technologies. Here, by incorporating ligand-responsive self-cleaving catalytic RNAs (aptazymes) into guide RNAs, we developed a set of aptazyme-embedded guide RNAs that enable small molecule-controlled nuclease-mediated genome editing and small molecule-controlled base editing, as well as small molecule-dependent transcriptional activation in mammalian cells. More... »

PAGES

15939

References to SciGraph publications

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  • 2007-09. Regulation of endogenous gene expression using small molecule-controlled engineered zinc-finger protein transcription factors in GENE THERAPY
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  • 2004-07. Engineered riboregulators enable post-transcriptional control of gene expression in NATURE BIOTECHNOLOGY
  • 2015-01. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo in NATURE BIOTECHNOLOGY
  • 2015-05. Small molecule–triggered Cas9 protein with improved genome-editing specificity in NATURE CHEMICAL BIOLOGY
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  • 2015-04. Highly efficient Cas9-mediated transcriptional programming in NATURE METHODS
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  • Journal

    TITLE

    Nature Communications

    ISSUE

    N/A

    VOLUME

    8

    Author Affiliations

    From Grant

  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncomms15939

    DOI

    http://dx.doi.org/10.1038/ncomms15939

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28656978


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    42 schema:description Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly advanced our ability to manipulate the human genome. Although canonical forms of genome-editing agents and programmable transcriptional regulators are constitutively active, precise temporal and spatial control over genome editing and transcriptional regulation activities would enable the more selective and potentially safer use of these powerful technologies. Here, by incorporating ligand-responsive self-cleaving catalytic RNAs (aptazymes) into guide RNAs, we developed a set of aptazyme-embedded guide RNAs that enable small molecule-controlled nuclease-mediated genome editing and small molecule-controlled base editing, as well as small molecule-dependent transcriptional activation in mammalian cells.
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