Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-06-06

AUTHORS

Youwei Xu, Carrie Bernecky, Chung-Tien Lee, Kerstin C. Maier, Björn Schwalb, Dimitry Tegunov, Jürgen M. Plitzko, Henning Urlaub, Patrick Cramer

ABSTRACT

The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin. More... »

PAGES

15741

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms15741

DOI

http://dx.doi.org/10.1038/ncomms15741

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085883147

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28585565


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding, Competitive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cross-Linking Reagents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryoelectron Microscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multiprotein Complexes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Polymerase II", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcriptional Elongation Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Youwei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bernecky", 
        "givenName": "Carrie", 
        "id": "sg:person.01170475633.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170475633.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, G\u00f6ttingen, Robert-Koch-Strasse 40, 37075, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.411984.1", 
          "name": [
            "Bioanalytical Mass Spectrometry, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
            "Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, G\u00f6ttingen, Robert-Koch-Strasse 40, 37075, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Chung-Tien", 
        "id": "sg:person.01231000477.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231000477.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maier", 
        "givenName": "Kerstin C.", 
        "id": "sg:person.01230060771.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230060771.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schwalb", 
        "givenName": "Bj\u00f6rn", 
        "id": "sg:person.0623344475.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623344475.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tegunov", 
        "givenName": "Dimitry", 
        "id": "sg:person.07417541233.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07417541233.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Plitzko", 
        "givenName": "J\u00fcrgen M.", 
        "id": "sg:person.0576723312.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576723312.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, G\u00f6ttingen, Robert-Koch-Strasse 40, 37075, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.411984.1", 
          "name": [
            "Bioanalytical Mass Spectrometry, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
            "Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, G\u00f6ttingen, Robert-Koch-Strasse 40, 37075, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Urlaub", 
        "givenName": "Henning", 
        "id": "sg:person.01264764341.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264764341.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany", 
          "id": "http://www.grid.ac/institutes/grid.418140.8", 
          "name": [
            "Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, G\u00f6ttingen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cramer", 
        "givenName": "Patrick", 
        "id": "sg:person.01045113153.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045113153.17"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nature09785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045811286", 
          "https://doi.org/10.1038/nature09785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature16482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018655005", 
          "https://doi.org/10.1038/nature16482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature17990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052707958", 
          "https://doi.org/10.1038/nature17990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1210582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013831372", 
          "https://doi.org/10.1038/sj.onc.1210582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041114691", 
          "https://doi.org/10.1038/nature14337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2099", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025908454", 
          "https://doi.org/10.1038/nmeth.2099"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.1903", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003457034", 
          "https://doi.org/10.1038/nsmb.1903"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-06-06", 
    "datePublishedReg": "2017-06-06", 
    "description": "The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/ncomms15741", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5493708", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043282", 
        "issn": [
          "2041-1723"
        ], 
        "name": "Nature Communications", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "keywords": [
      "elongation factor TFIIS", 
      "transcription elongation complex", 
      "elongation complex", 
      "RNA polymerase II transcription elongation complexes", 
      "polymerase-associated factor 1 complex", 
      "Pol II elongation complex", 
      "Pol II surface", 
      "factor 1 complex", 
      "cryo-electron microscopy", 
      "chromatin transcription", 
      "Pol II", 
      "Paf1C", 
      "genomic regulation", 
      "RNA polymerase", 
      "mRNA transcription", 
      "transcription", 
      "TFIIS", 
      "multiple roles", 
      "complexes", 
      "Leo1", 
      "PAF1", 
      "Rtf1", 
      "Ctr9", 
      "chromatin", 
      "Saccharomyces", 
      "structural analysis", 
      "structural observations", 
      "yeast", 
      "Further analysis", 
      "polymerase", 
      "heterodimers", 
      "subunits", 
      "CDC73", 
      "binds", 
      "regulation", 
      "reconstitution", 
      "impairs", 
      "role", 
      "function", 
      "analysis", 
      "microscopy", 
      "form", 
      "observations", 
      "end", 
      "architecture", 
      "bridge", 
      "data", 
      "results", 
      "surface", 
      "framework", 
      "three-dimensional framework"
    ], 
    "name": "Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex", 
    "pagination": "15741", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1085883147"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ncomms15741"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28585565"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ncomms15741", 
      "https://app.dimensions.ai/details/publication/pub.1085883147"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T17:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_735.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/ncomms15741"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncomms15741'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncomms15741'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncomms15741'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncomms15741'


 

This table displays all metadata directly associated to this object as RDF triples.

255 TRIPLES      21 PREDICATES      96 URIs      81 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ncomms15741 schema:about N0742bfc7dfb74cdbb5c1d38674355c01
2 N2b7b247c3d4041d2af6d4a21eb2c962b
3 N36102d7b843f414ca6f97a2cdedc906e
4 N366bca40d88c4d70b731e8c010ce886d
5 N569ccd394a1f4a858f0e4f94b6576a2a
6 N6fd21e5baf1b4bcd9e1cac31d8178267
7 N82b84346f1eb4513a8ed25181415753c
8 Na082f4ae167a47c597fcf9e649ae5999
9 Na7325b152dbe4d898a08d5418029192c
10 Ne717d95a23dc49279c77de392394ed09
11 Nf013fba3b13346bab42ccffed50b60af
12 Nf6ecd2e100f84808801f97b439250da8
13 Nf8b03998020848d8b44722aa0a9a0bd5
14 anzsrc-for:06
15 anzsrc-for:0601
16 schema:author N936a45e90f8146a4898e42121daa6ebf
17 schema:citation sg:pub.10.1038/nature09785
18 sg:pub.10.1038/nature14337
19 sg:pub.10.1038/nature16482
20 sg:pub.10.1038/nature17990
21 sg:pub.10.1038/nmeth.2099
22 sg:pub.10.1038/nsmb.1903
23 sg:pub.10.1038/sj.onc.1210582
24 schema:datePublished 2017-06-06
25 schema:datePublishedReg 2017-06-06
26 schema:description The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin.
27 schema:genre article
28 schema:isAccessibleForFree true
29 schema:isPartOf N14da3fbbcf5a4015a3edc65e13003cdb
30 Nf37436a8e4ec4ae98fad2f31985cf3a4
31 sg:journal.1043282
32 schema:keywords CDC73
33 Ctr9
34 Further analysis
35 Leo1
36 PAF1
37 Paf1C
38 Pol II
39 Pol II elongation complex
40 Pol II surface
41 RNA polymerase
42 RNA polymerase II transcription elongation complexes
43 Rtf1
44 Saccharomyces
45 TFIIS
46 analysis
47 architecture
48 binds
49 bridge
50 chromatin
51 chromatin transcription
52 complexes
53 cryo-electron microscopy
54 data
55 elongation complex
56 elongation factor TFIIS
57 end
58 factor 1 complex
59 form
60 framework
61 function
62 genomic regulation
63 heterodimers
64 impairs
65 mRNA transcription
66 microscopy
67 multiple roles
68 observations
69 polymerase
70 polymerase-associated factor 1 complex
71 reconstitution
72 regulation
73 results
74 role
75 structural analysis
76 structural observations
77 subunits
78 surface
79 three-dimensional framework
80 transcription
81 transcription elongation complex
82 yeast
83 schema:name Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex
84 schema:pagination 15741
85 schema:productId N36358fc867054ea69dab7c2c6cf12b21
86 N5316b698a537437b98f8fc1cec8069e2
87 N86491ee7b6a942eeb5831ef91ce86bb6
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085883147
89 https://doi.org/10.1038/ncomms15741
90 schema:sdDatePublished 2022-08-04T17:06
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher N12fa1e2ad3264ef19e1afe9431fec615
93 schema:url https://doi.org/10.1038/ncomms15741
94 sgo:license sg:explorer/license/
95 sgo:sdDataset articles
96 rdf:type schema:ScholarlyArticle
97 N0742bfc7dfb74cdbb5c1d38674355c01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Nuclear Proteins
99 rdf:type schema:DefinedTerm
100 N12fa1e2ad3264ef19e1afe9431fec615 schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 N14da3fbbcf5a4015a3edc65e13003cdb schema:issueNumber 1
103 rdf:type schema:PublicationIssue
104 N28afc89635334fabbc6c81676f8ac61c rdf:first sg:person.0576723312.32
105 rdf:rest N956c135935d24f35b73c1d37868e6111
106 N2b7b247c3d4041d2af6d4a21eb2c962b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Cell Cycle Proteins
108 rdf:type schema:DefinedTerm
109 N36102d7b843f414ca6f97a2cdedc906e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Recombinant Proteins
111 rdf:type schema:DefinedTerm
112 N36358fc867054ea69dab7c2c6cf12b21 schema:name doi
113 schema:value 10.1038/ncomms15741
114 rdf:type schema:PropertyValue
115 N366bca40d88c4d70b731e8c010ce886d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Protein Conformation
117 rdf:type schema:DefinedTerm
118 N410c3f6c08cd4b9d94e0c542d50af1c4 rdf:first sg:person.0623344475.06
119 rdf:rest Nc8991bca57b14d40a574564f2020b48a
120 N5316b698a537437b98f8fc1cec8069e2 schema:name pubmed_id
121 schema:value 28585565
122 rdf:type schema:PropertyValue
123 N569ccd394a1f4a858f0e4f94b6576a2a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Multiprotein Complexes
125 rdf:type schema:DefinedTerm
126 N6fd21e5baf1b4bcd9e1cac31d8178267 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Transcription, Genetic
128 rdf:type schema:DefinedTerm
129 N7d5556a8a2ed433a92f0563384a3f144 rdf:first sg:person.01231000477.17
130 rdf:rest N81cd03e511de4cb089cf82b86638b161
131 N815262ed5a2a4879a1c050f3ecc6eef4 rdf:first sg:person.01170475633.64
132 rdf:rest N7d5556a8a2ed433a92f0563384a3f144
133 N81cd03e511de4cb089cf82b86638b161 rdf:first sg:person.01230060771.79
134 rdf:rest N410c3f6c08cd4b9d94e0c542d50af1c4
135 N82b84346f1eb4513a8ed25181415753c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name RNA-Binding Proteins
137 rdf:type schema:DefinedTerm
138 N86491ee7b6a942eeb5831ef91ce86bb6 schema:name dimensions_id
139 schema:value pub.1085883147
140 rdf:type schema:PropertyValue
141 N936a45e90f8146a4898e42121daa6ebf rdf:first Na07130b818d648dfa8ecf3026b6a2dde
142 rdf:rest N815262ed5a2a4879a1c050f3ecc6eef4
143 N956c135935d24f35b73c1d37868e6111 rdf:first sg:person.01264764341.85
144 rdf:rest Na4b4d34ff44e4cb7ac3804f94308be71
145 Na07130b818d648dfa8ecf3026b6a2dde schema:affiliation grid-institutes:grid.418140.8
146 schema:familyName Xu
147 schema:givenName Youwei
148 rdf:type schema:Person
149 Na082f4ae167a47c597fcf9e649ae5999 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Cryoelectron Microscopy
151 rdf:type schema:DefinedTerm
152 Na4b4d34ff44e4cb7ac3804f94308be71 rdf:first sg:person.01045113153.17
153 rdf:rest rdf:nil
154 Na7325b152dbe4d898a08d5418029192c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Saccharomyces cerevisiae Proteins
156 rdf:type schema:DefinedTerm
157 Nc8991bca57b14d40a574564f2020b48a rdf:first sg:person.07417541233.11
158 rdf:rest N28afc89635334fabbc6c81676f8ac61c
159 Ne717d95a23dc49279c77de392394ed09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Transcriptional Elongation Factors
161 rdf:type schema:DefinedTerm
162 Nf013fba3b13346bab42ccffed50b60af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Binding, Competitive
164 rdf:type schema:DefinedTerm
165 Nf37436a8e4ec4ae98fad2f31985cf3a4 schema:volumeNumber 8
166 rdf:type schema:PublicationVolume
167 Nf6ecd2e100f84808801f97b439250da8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Cross-Linking Reagents
169 rdf:type schema:DefinedTerm
170 Nf8b03998020848d8b44722aa0a9a0bd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name RNA Polymerase II
172 rdf:type schema:DefinedTerm
173 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
174 schema:name Biological Sciences
175 rdf:type schema:DefinedTerm
176 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
177 schema:name Biochemistry and Cell Biology
178 rdf:type schema:DefinedTerm
179 sg:grant.5493708 http://pending.schema.org/fundedItem sg:pub.10.1038/ncomms15741
180 rdf:type schema:MonetaryGrant
181 sg:journal.1043282 schema:issn 2041-1723
182 schema:name Nature Communications
183 schema:publisher Springer Nature
184 rdf:type schema:Periodical
185 sg:person.01045113153.17 schema:affiliation grid-institutes:grid.418140.8
186 schema:familyName Cramer
187 schema:givenName Patrick
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045113153.17
189 rdf:type schema:Person
190 sg:person.01170475633.64 schema:affiliation grid-institutes:grid.418140.8
191 schema:familyName Bernecky
192 schema:givenName Carrie
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170475633.64
194 rdf:type schema:Person
195 sg:person.01230060771.79 schema:affiliation grid-institutes:grid.418140.8
196 schema:familyName Maier
197 schema:givenName Kerstin C.
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230060771.79
199 rdf:type schema:Person
200 sg:person.01231000477.17 schema:affiliation grid-institutes:grid.411984.1
201 schema:familyName Lee
202 schema:givenName Chung-Tien
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231000477.17
204 rdf:type schema:Person
205 sg:person.01264764341.85 schema:affiliation grid-institutes:grid.411984.1
206 schema:familyName Urlaub
207 schema:givenName Henning
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264764341.85
209 rdf:type schema:Person
210 sg:person.0576723312.32 schema:affiliation grid-institutes:grid.418615.f
211 schema:familyName Plitzko
212 schema:givenName Jürgen M.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576723312.32
214 rdf:type schema:Person
215 sg:person.0623344475.06 schema:affiliation grid-institutes:grid.418140.8
216 schema:familyName Schwalb
217 schema:givenName Björn
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623344475.06
219 rdf:type schema:Person
220 sg:person.07417541233.11 schema:affiliation grid-institutes:grid.418140.8
221 schema:familyName Tegunov
222 schema:givenName Dimitry
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07417541233.11
224 rdf:type schema:Person
225 sg:pub.10.1038/nature09785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045811286
226 https://doi.org/10.1038/nature09785
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nature14337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041114691
229 https://doi.org/10.1038/nature14337
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nature16482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018655005
232 https://doi.org/10.1038/nature16482
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nature17990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052707958
235 https://doi.org/10.1038/nature17990
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/nmeth.2099 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025908454
238 https://doi.org/10.1038/nmeth.2099
239 rdf:type schema:CreativeWork
240 sg:pub.10.1038/nsmb.1903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003457034
241 https://doi.org/10.1038/nsmb.1903
242 rdf:type schema:CreativeWork
243 sg:pub.10.1038/sj.onc.1210582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013831372
244 https://doi.org/10.1038/sj.onc.1210582
245 rdf:type schema:CreativeWork
246 grid-institutes:grid.411984.1 schema:alternateName Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, Göttingen, Robert-Koch-Strasse 40, 37075, Göttingen, Germany
247 schema:name Bioanalytical Mass Spectrometry, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany
248 Bioanalytics Group, Institute for Clinical Chemistry, University Medical Center, Göttingen, Robert-Koch-Strasse 40, 37075, Göttingen, Germany
249 rdf:type schema:Organization
250 grid-institutes:grid.418140.8 schema:alternateName Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, Göttingen, Germany
251 schema:name Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, Max Planck Society, Am Fassberg 11, 37077, Göttingen, Germany
252 rdf:type schema:Organization
253 grid-institutes:grid.418615.f schema:alternateName Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany
254 schema:name Department of Molecular Structural Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany
255 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...