The microprotein Minion controls cell fusion and muscle formation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-06-01

AUTHORS

Qiao Zhang, Ajay A. Vashisht, Jason O'Rourke, Stéphane Y Corbel, Rita Moran, Angelica Romero, Loren Miraglia, Jia Zhang, Eric Durrant, Christian Schmedt, Srinath C. Sampath, Srihari C. Sampath

ABSTRACT

Although recent evidence has pointed to the existence of small open reading frame (smORF)-encoded microproteins in mammals, their function remains to be determined. Skeletal muscle development requires fusion of mononuclear progenitors to form multinucleated myotubes, a critical but poorly understood process. Here we report the identification of Minion (microprotein inducer of fusion), a smORF encoding an essential skeletal muscle specific microprotein. Myogenic progenitors lacking Minion differentiate normally but fail to form syncytial myotubes, and Minion-deficient mice die perinatally and demonstrate a marked reduction in fused muscle fibres. The fusogenic activity of Minion is conserved in the human orthologue, and co-expression of Minion and the transmembrane protein Myomaker is sufficient to induce cellular fusion accompanied by rapid cytoskeletal rearrangement, even in non-muscle cells. These findings establish Minion as a novel microprotein required for muscle development, and define a two-component programme for the induction of mammalian cell fusion. Moreover, these data also significantly expand the known functions of smORF-encoded microproteins. More... »

PAGES

15664

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms15664

DOI

http://dx.doi.org/10.1038/ncomms15664

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085733393

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28569745


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34 schema:description Although recent evidence has pointed to the existence of small open reading frame (smORF)-encoded microproteins in mammals, their function remains to be determined. Skeletal muscle development requires fusion of mononuclear progenitors to form multinucleated myotubes, a critical but poorly understood process. Here we report the identification of Minion (microprotein inducer of fusion), a smORF encoding an essential skeletal muscle specific microprotein. Myogenic progenitors lacking Minion differentiate normally but fail to form syncytial myotubes, and Minion-deficient mice die perinatally and demonstrate a marked reduction in fused muscle fibres. The fusogenic activity of Minion is conserved in the human orthologue, and co-expression of Minion and the transmembrane protein Myomaker is sufficient to induce cellular fusion accompanied by rapid cytoskeletal rearrangement, even in non-muscle cells. These findings establish Minion as a novel microprotein required for muscle development, and define a two-component programme for the induction of mammalian cell fusion. Moreover, these data also significantly expand the known functions of smORF-encoded microproteins.
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42 Minion-deficient mice
43 Recent evidence
44 activity
45 cell fusion
46 cells
47 cellular fusion
48 cytoskeletal rearrangements
49 data
50 development
51 essential skeletal muscle specific microprotein
52 evidence
53 existence
54 fibers
55 findings
56 formation
57 frame
58 function
59 functions of smORF
60 fused muscle fibres
61 fusion
62 fusogenic activity
63 human orthologue
64 identification
65 identification of Minion
66 induction
67 mammalian cell fusion
68 mammals
69 marked reduction
70 mice
71 microprotein Minion
72 microproteins
73 mononuclear progenitors
74 muscle development
75 muscle fibers
76 muscle formation
77 muscle specific microprotein
78 myogenic progenitors
79 myomaker
80 myotubes
81 non-muscle cells
82 novel microprotein
83 open reading frame
84 orthologues
85 process
86 progenitors
87 program
88 protein Myomaker
89 rapid cytoskeletal rearrangement
90 reading frame
91 rearrangement
92 reduction
93 skeletal muscle development
94 skeletal muscle specific microprotein
95 smORFs
96 small open reading frames
97 specific microprotein
98 syncytial myotubes
99 transmembrane protein Myomaker
100 two-component programme
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