Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-05-03

AUTHORS

Han-Yi Fu, Daniel Picot, Yves Choquet, Guillaume Longatte, Adnan Sayegh, Jérôme Delacotte, Manon Guille-Collignon, Frédéric Lemaître, Fabrice Rappaport, Francis-André Wollman

ABSTRACT

Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor QA of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the QA site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between QA and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural QA°- to QB pathway. More... »

PAGES

15274

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms15274

DOI

http://dx.doi.org/10.1038/ncomms15274

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085122916

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28466860


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31 schema:description Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor Q<sub>A</sub> of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the Q<sub>A</sub> site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between Q<sub>A</sub> and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural Q<sub>A</sub>°<sup>-</sup> to Q<sub>B</sub> pathway.
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39 DMBQ
40 PSII mutants
41 PsbT subunit
42 QA
43 acceptor Q
44 alga Chlamydomonas reinhardtii
45 benzoquinone
46 bypass
47 chronoamperometry
48 dimethyl
49 distance
50 donation pathway
51 efficiency
52 efficiency of mutation
53 electrical power
54 electron acceptor Q
55 electron donation pathway
56 electron flow
57 electron mediator
58 electron transfer
59 environment
60 evidence
61 exogenous quinones
62 flow
63 green alga Chlamydomonas reinhardtii
64 mediators
65 mutants
66 mutations
67 novel electron donation pathway
68 organisms
69 pathway
70 photosynthesis
71 photosynthetic electron flow
72 photosynthetic organisms
73 photosystem II
74 phototrophic properties
75 power
76 prediction studies
77 primary electron acceptor Q
78 properties
79 quinone
80 rate
81 reduction
82 reduction rate
83 reinhardtii
84 screen
85 site-directed PSII mutants
86 sites
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88 stroma
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