A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI heads View Full Text


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Article Info

DATE

2016-12-12

AUTHORS

M. Iwaki, S. F. Wickham, K. Ikezaki, T. Yanagida, W. M. Shih

ABSTRACT

Mechanosensitive biological nanomachines such as motor proteins and ion channels regulate diverse cellular behaviour. Combined optical trapping with single-molecule fluorescence imaging provides a powerful methodology to clearly characterize the mechanoresponse, structural dynamics and stability of such nanomachines. However, this system requires complicated experimental geometry, preparation and optics, and is limited by low data-acquisition efficiency. Here we develop a programmable DNA origami nanospring that overcomes these issues. We apply our nanospring to human myosin VI, a mechanosensory motor protein, and demonstrate nanometre-precision single-molecule fluorescence imaging of the individual motor domains (heads) under force. We observe force-induced transitions of myosin VI heads from non-adjacent to adjacent binding, which correspond to adapted roles for low-load and high-load transport, respectively. Our technique extends single-molecule studies under force and clarifies the effect of force on biological processes. More... »

PAGES

13715

References to SciGraph publications

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  • 2006-10-01. Single-molecule observations of neck linker conformational changes in the kinesin motor protein in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2003-03. Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization in NATURE
  • 2009-05-10. Brownian search-and-catch mechanism for myosin-VI steps in NATURE CHEMICAL BIOLOGY
  • 1993-10. Direct observation of kinesin stepping by optical trapping interferometry in NATURE
  • 2007-06. Mechanosensitive channels in bacteria: signs of closure? in NATURE REVIEWS MICROBIOLOGY
  • 2012-01-08. Dynein achieves processive motion using both stochastic and coordinated stepping in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 1987-04-01. Sliding movement of single actin filaments on one-headed myosin filaments in NATURE
  • 2011-12-07. Walking to work: roles for class V myosins as cargo transporters in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2016-02-29. Direct observation of intermediate states during the stepping motion of kinesin-1 in NATURE CHEMICAL BIOLOGY
  • 2005-10. Repetitive shuttling of a motor protein on DNA in NATURE
  • 2008-05-30. Long single α-helical tail domains bridge the gap between structure and function of myosin VI in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncomms13715

    DOI

    http://dx.doi.org/10.1038/ncomms13715

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015922542

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27941751


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