Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-07-22

AUTHORS

Nambin Yim, Seung-Wook Ryu, Kyungsun Choi, Kwang Ryeol Lee, Seunghee Lee, Hojun Choi, Jeongjin Kim, Mohammed R. Shaker, Woong Sun, Ji-Ho Park, Daesoo Kim, Won Do Heo, Chulhee Choi

ABSTRACT

Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues. More... »

PAGES

12277

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms12277

DOI

http://dx.doi.org/10.1038/ncomms12277

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005825082

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27447450


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

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curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncomms12277'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncomms12277'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncomms12277'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncomms12277'


 

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322 Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34047, Korea
323 Department of Biological Sciences, KAIST, Daejeon 34141, Korea
324 rdf:type schema:Organization
325 https://www.grid.ac/institutes/grid.452901.b schema:alternateName Ministry of Education
326 schema:name Department of Anatomy, Brain Korea 21 Program, Korea University College of Medicine, Seoul 02841, Korea
327 rdf:type schema:Organization
 




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