Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04-20

AUTHORS

Alexandra Friese, Alex C. Faesen, Pim J. Huis in ‘t Veld, Josef Fischböck, Daniel Prumbaum, Arsen Petrovic, Stefan Raunser, Franz Herzog, Andrea Musacchio

ABSTRACT

Accurate chromosome segregation during cell division is crucial for propagating life and protects from cellular transformation. The SKAP:Astrin heterodimer localizes to spindle microtubules and to mature microtubule–kinetochore attachments during mitosis. Depletion of either subunit disrupts spindle structure and destabilizes kinetochore–microtubule attachments. Here, we identify molecular requirements for the inter-subunit interaction of SKAP and Astrin, and discuss requirements for their kinetochore recruitment. We also identify and characterize a microtubule-binding domain in SKAP, distinct from the SXIP motif that mediates end binding (EB) protein binding and plus end tracking, and show that it stimulates the growth-rate of microtubules, possibly through a direct interaction with tubulin. Mutations targeting this microtubule-binding domain impair microtubule plus-end tracking but not kinetochore targeting, and recapitulate many effects observed during depletion of SKAP. Collectively, our studies represent the first thorough mechanistic analysis of SKAP and Astrin, and significantly advance our functional understanding of these important mitotic proteins. More... »

PAGES

11407

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncomms11407

DOI

http://dx.doi.org/10.1038/ncomms11407

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030261235

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27095104


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84 segregation
85 spindle structure
86 structure
87 study
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89 tracking
90 transformation
91 tubulin
92 understanding
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