Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04-18

AUTHORS

Cuicui Shen, Delin Zhang, Zeyuan Guan, Yexing Liu, Zhao Yang, Yan Yang, Xiang Wang, Qiang Wang, QunXia Zhang, Shilong Fan, Tingting Zou, Ping Yin

ABSTRACT

As a large family of RNA-binding proteins, pentatricopeptide repeat (PPR) proteins mediate multiple aspects of RNA metabolism in eukaryotes. Binding to their target single-stranded RNAs (ssRNAs) in a modular and base-specific fashion, PPR proteins can serve as designable modules for gene manipulation. However, the structural basis for nucleotide-specific recognition by designer PPR (dPPR) proteins remains to be elucidated. Here, we report four crystal structures of dPPR proteins in complex with their respective ssRNA targets. The dPPR repeats are assembled into a right-handed superhelical spiral shell that embraces the ssRNA. Interactions between different PPR codes and RNA bases are observed at the atomic level, revealing the molecular basis for the modular and specific recognition patterns of the RNA bases U, C, A and G. These structures not only provide insights into the functional study of PPR proteins but also open a path towards the potential design of synthetic sequence-specific RNA-binding proteins. More... »

PAGES

11285

References to SciGraph publications

  • 2014-12. An artificial PPR scaffold for programmable RNA recognition in NATURE COMMUNICATIONS
  • 2013-12. Structural basis for the modular recognition of single-stranded RNA by PPR proteins in NATURE
  • 2015-08. Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome in NATURE CHEMICAL BIOLOGY
  • 2014-08. A protein-RNA specificity code enables targeted activation of an endogenous human transcript in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2014-11. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells in NATURE
  • 2007-04-01. Imaging dynamics of endogenous mitochondrial RNA in single living cells in NATURE METHODS
  • 2013-11. Structure of human mitochondrial RNA polymerase elongation complex in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2015-12. Rational design of α-helical tandem repeat proteins with closed architectures in NATURE
  • 2012-01. Zinc-finger nucleases: how to play two good hands in NATURE METHODS
  • 2014-04. Revisiting the TALE repeat in PROTEIN & CELL
  • 2013-12. Structural basis for RNA recognition by a dimeric PPR-protein complex in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2015-12. Exploring the repeat protein universe through computational protein design in NATURE
  • 2012-11. In vivo genome editing using a high-efficiency TALEN system in NATURE
  • 2005-01. A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts in NATURE
  • 2011-07. A universal code for RNA recognition by PUF proteins in NATURE CHEMICAL BIOLOGY
  • 2011-10. Structure of human mitochondrial RNA polymerase in NATURE
  • 2015-05. High-throughput functional genomics using CRISPR–Cas9 in NATURE REVIEWS GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncomms11285

    DOI

    http://dx.doi.org/10.1038/ncomms11285

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050889558

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27088764


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