Dynamic imaging of protease activity with fluorescently quenched activity-based probes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-09

AUTHORS

Galia Blum, Stefanie R Mullins, Kinneret Keren, Marko Fonovič, Christopher Jedeszko, Mark J Rice, Bonnie F Sloane, Matthew Bogyo

ABSTRACT

Protease activity is tightly regulated in both normal and disease conditions. However, it is often difficult to monitor the dynamic nature of this regulation in the context of a live cell or whole organism. To address this limitation, we developed a series of quenched activity-based probes (qABPs) that become fluorescent upon activity-dependent covalent modification of a protease target. These reagents freely penetrate cells and allow direct imaging of protease activity in living cells. Targeted proteases are directly identified and monitored biochemically by virtue of the resulting covalent tag, thereby allowing unambiguous assignment of protease activities observed in imaging studies. We report here the design and synthesis of a selective, cell-permeable qABP for the study of papain-family cysteine proteases. This probe is used to monitor real-time protease activity in live human cells with fluorescence microscopy techniques as well as standard biochemical methods. More... »

PAGES

203

References to SciGraph publications

  • 2005-06. Activity-based probes that target diverse cysteine protease families in NATURE CHEMICAL BIOLOGY
  • 2003-07. Human and mouse proteases: a comparative genomic approach in NATURE REVIEWS GENETICS
  • 2004-12. Activity-Based Protein Profiling in AMERICAN JOURNAL OF PHARMACOGENOMICS
  • 2002-07. Building epithelial architecture: insights from three-dimensional culture models in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nchembio728

    DOI

    http://dx.doi.org/10.1038/nchembio728

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1038345583

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16408036


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nchembio728'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nchembio728'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nchembio728'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nchembio728'


     

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