Small molecule–triggered Cas9 protein with improved genome-editing specificity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-05

AUTHORS

Kevin M Davis, Vikram Pattanayak, David B Thompson, John A Zuris, David R Liu

ABSTRACT

Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9. More... »

PAGES

316-318

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nchembio.1793

DOI

http://dx.doi.org/10.1038/nchembio.1793

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042535273

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25848930


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