Structure of a PEGylated protein reveals a highly porous double-helical assembly View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-10

AUTHORS

Giada Cattani, Lutz Vogeley, Peter B. Crowley

ABSTRACT

PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model β-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies. More... »

PAGES

823-828

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nchem.2342

DOI

http://dx.doi.org/10.1038/nchem.2342

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045322351

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26391082


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0303", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Macromolecular and Materials Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Magnetic Resonance, Biomolecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polyethylene Glycols", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National University of Ireland, Galway", 
          "id": "https://www.grid.ac/institutes/grid.6142.1", 
          "name": [
            "School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cattani", 
        "givenName": "Giada", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Trinity College Dublin", 
          "id": "https://www.grid.ac/institutes/grid.8217.c", 
          "name": [
            "School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vogeley", 
        "givenName": "Lutz", 
        "id": "sg:person.01127661141.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127661141.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of Ireland, Galway", 
          "id": "https://www.grid.ac/institutes/grid.6142.1", 
          "name": [
            "School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crowley", 
        "givenName": "Peter B.", 
        "id": "sg:person.01175774341.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175774341.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrd1033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000722784", 
          "https://doi.org/10.1038/nrd1033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd1033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000722784", 
          "https://doi.org/10.1038/nrd1033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3sc22015j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002027352"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560010907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004894742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.107.114314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006742326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.21482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009525756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444902014439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012218185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889807021206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012753217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/chem.200901410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013231151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/chem.200901410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013231151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1011254402785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013814896", 
          "https://doi.org/10.1023/a:1011254402785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jconrel.2011.10.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013971739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp9058966", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015196255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp9058966", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015196255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.106.097717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015730911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444904019158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020281098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(00)00065-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020923852"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cbic.200700603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021870015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444911047834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022229088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm200012073432301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022881380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201405664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025569457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444994003112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025573949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.37.032807.125817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026164216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.20449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027764068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.108.133025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029867983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nchem.2107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030878022", 
          "https://doi.org/10.1038/nchem.2107"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444911001314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jps.21170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036429275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2010.01.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037874026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0142-9612(98)00007-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038121526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.16.8399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040019333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201206563", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041416479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja209653n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047131688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc2003583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050471136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc2003583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050471136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.200462415", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051738232"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.200902672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051926603"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-391927-4.10024-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052518752"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc049781+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc049781+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc049843w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc049843w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc9003052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055155858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc9003052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055155858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi800125h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi800125h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bm100999a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055221766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bm100999a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055221766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bm300561y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055222462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja021264j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055826972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja021264j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055826972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja900786z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055860693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja900786z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055860693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja9942787", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055873422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ma60077a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056201934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/mp200470c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056212725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1524/zksu.2006.suppl_23.613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067652420"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077283373", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083331425", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-10", 
    "datePublishedReg": "2015-10-01", 
    "description": "PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model \u03b2-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nchem.2342", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3984296", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3984571", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1041224", 
        "issn": [
          "1755-4330", 
          "1755-4349"
        ], 
        "name": "Nature Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Structure of a PEGylated protein reveals a highly porous double-helical assembly", 
    "pagination": "823-828", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d7e37e3150bf3e927401df4635d9b10be9ddb5cf0f4a3c7ee6cc9b68653e5d2f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26391082"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101499734"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nchem.2342"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045322351"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nchem.2342", 
      "https://app.dimensions.ai/details/publication/pub.1045322351"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nchem/journal/v7/n10/full/nchem.2342.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nchem.2342'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nchem.2342'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nchem.2342'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nchem.2342'


 

This table displays all metadata directly associated to this object as RDF triples.

254 TRIPLES      21 PREDICATES      82 URIs      25 LITERALS      13 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nchem.2342 schema:about N31ab53d64958454daf1d69b567eb56ea
2 N5036852022234dc78ea00adc5d35381b
3 N5f0a1f0740ac44a69a45d5071788ec5a
4 N7642fc17db3f4e8aa8aea049bb55b58f
5 anzsrc-for:03
6 anzsrc-for:0303
7 schema:author N809de09998444420a4ba05d234260303
8 schema:citation sg:pub.10.1007/bf00197809
9 sg:pub.10.1023/a:1011254402785
10 sg:pub.10.1038/nchem.2107
11 sg:pub.10.1038/nrd1033
12 https://app.dimensions.ai/details/publication/pub.1077283373
13 https://app.dimensions.ai/details/publication/pub.1083331425
14 https://doi.org/10.1002/anie.200462415
15 https://doi.org/10.1002/anie.200902672
16 https://doi.org/10.1002/anie.201206563
17 https://doi.org/10.1002/anie.201405664
18 https://doi.org/10.1002/bit.21482
19 https://doi.org/10.1002/cbic.200700603
20 https://doi.org/10.1002/chem.200901410
21 https://doi.org/10.1002/jps.21170
22 https://doi.org/10.1002/pro.5560010907
23 https://doi.org/10.1002/prot.20449
24 https://doi.org/10.1016/b978-0-12-391927-4.10024-6
25 https://doi.org/10.1016/j.jconrel.2011.10.037
26 https://doi.org/10.1016/j.sbi.2010.01.008
27 https://doi.org/10.1016/s0142-9612(98)00007-6
28 https://doi.org/10.1016/s0969-2126(00)00065-4
29 https://doi.org/10.1021/bc049781+
30 https://doi.org/10.1021/bc049843w
31 https://doi.org/10.1021/bc2003583
32 https://doi.org/10.1021/bc9003052
33 https://doi.org/10.1021/bi800125h
34 https://doi.org/10.1021/bm100999a
35 https://doi.org/10.1021/bm300561y
36 https://doi.org/10.1021/ja021264j
37 https://doi.org/10.1021/ja209653n
38 https://doi.org/10.1021/ja900786z
39 https://doi.org/10.1021/ja9942787
40 https://doi.org/10.1021/jp9058966
41 https://doi.org/10.1021/ma60077a009
42 https://doi.org/10.1021/mp200470c
43 https://doi.org/10.1039/c3sc22015j
44 https://doi.org/10.1056/nejm200012073432301
45 https://doi.org/10.1073/pnas.94.16.8399
46 https://doi.org/10.1107/s0021889807021206
47 https://doi.org/10.1107/s0907444902014439
48 https://doi.org/10.1107/s0907444904019158
49 https://doi.org/10.1107/s0907444911001314
50 https://doi.org/10.1107/s0907444911047834
51 https://doi.org/10.1107/s0907444994003112
52 https://doi.org/10.1146/annurev.biophys.37.032807.125817
53 https://doi.org/10.1524/zksu.2006.suppl_23.613
54 https://doi.org/10.1529/biophysj.106.097717
55 https://doi.org/10.1529/biophysj.107.114314
56 https://doi.org/10.1529/biophysj.108.133025
57 schema:datePublished 2015-10
58 schema:datePublishedReg 2015-10-01
59 schema:description PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model β-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree false
63 schema:isPartOf Ncd942fbe4bac4a6aade3068c6b74e703
64 Nea3c9216d77c40c4bb705a8244f0bee1
65 sg:journal.1041224
66 schema:name Structure of a PEGylated protein reveals a highly porous double-helical assembly
67 schema:pagination 823-828
68 schema:productId N335185c1aa244893b0bb1ece4d8fee41
69 N4ca023f8503f49d0b9167c3459bbfc26
70 N8d815709b8874f8daee732db8d1bcb78
71 Neb29fe5cbbb04d00ad4929c22b575fff
72 Nf66897889bf74481b06e85b53610db6f
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045322351
74 https://doi.org/10.1038/nchem.2342
75 schema:sdDatePublished 2019-04-10T13:56
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher Nacb03d39de0240b4942a5a4e14163ddc
78 schema:url http://www.nature.com/nchem/journal/v7/n10/full/nchem.2342.html
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N31ab53d64958454daf1d69b567eb56ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Protein Conformation
84 rdf:type schema:DefinedTerm
85 N335185c1aa244893b0bb1ece4d8fee41 schema:name doi
86 schema:value 10.1038/nchem.2342
87 rdf:type schema:PropertyValue
88 N4ca023f8503f49d0b9167c3459bbfc26 schema:name nlm_unique_id
89 schema:value 101499734
90 rdf:type schema:PropertyValue
91 N5036852022234dc78ea00adc5d35381b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Nuclear Magnetic Resonance, Biomolecular
93 rdf:type schema:DefinedTerm
94 N5f0a1f0740ac44a69a45d5071788ec5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Proteins
96 rdf:type schema:DefinedTerm
97 N7642fc17db3f4e8aa8aea049bb55b58f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Polyethylene Glycols
99 rdf:type schema:DefinedTerm
100 N809de09998444420a4ba05d234260303 rdf:first Ne1e27f0920324eaaa7edf8bc6ad2f885
101 rdf:rest Ne699635ade494e27b6266646470ddbdb
102 N8d815709b8874f8daee732db8d1bcb78 schema:name pubmed_id
103 schema:value 26391082
104 rdf:type schema:PropertyValue
105 Na77ea5357d1a4e03b8a27ea95838e696 rdf:first sg:person.01175774341.29
106 rdf:rest rdf:nil
107 Nacb03d39de0240b4942a5a4e14163ddc schema:name Springer Nature - SN SciGraph project
108 rdf:type schema:Organization
109 Ncd942fbe4bac4a6aade3068c6b74e703 schema:issueNumber 10
110 rdf:type schema:PublicationIssue
111 Ne1e27f0920324eaaa7edf8bc6ad2f885 schema:affiliation https://www.grid.ac/institutes/grid.6142.1
112 schema:familyName Cattani
113 schema:givenName Giada
114 rdf:type schema:Person
115 Ne699635ade494e27b6266646470ddbdb rdf:first sg:person.01127661141.45
116 rdf:rest Na77ea5357d1a4e03b8a27ea95838e696
117 Nea3c9216d77c40c4bb705a8244f0bee1 schema:volumeNumber 7
118 rdf:type schema:PublicationVolume
119 Neb29fe5cbbb04d00ad4929c22b575fff schema:name readcube_id
120 schema:value d7e37e3150bf3e927401df4635d9b10be9ddb5cf0f4a3c7ee6cc9b68653e5d2f
121 rdf:type schema:PropertyValue
122 Nf66897889bf74481b06e85b53610db6f schema:name dimensions_id
123 schema:value pub.1045322351
124 rdf:type schema:PropertyValue
125 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
126 schema:name Chemical Sciences
127 rdf:type schema:DefinedTerm
128 anzsrc-for:0303 schema:inDefinedTermSet anzsrc-for:
129 schema:name Macromolecular and Materials Chemistry
130 rdf:type schema:DefinedTerm
131 sg:grant.3984296 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.2342
132 rdf:type schema:MonetaryGrant
133 sg:grant.3984571 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.2342
134 rdf:type schema:MonetaryGrant
135 sg:journal.1041224 schema:issn 1755-4330
136 1755-4349
137 schema:name Nature Chemistry
138 rdf:type schema:Periodical
139 sg:person.01127661141.45 schema:affiliation https://www.grid.ac/institutes/grid.8217.c
140 schema:familyName Vogeley
141 schema:givenName Lutz
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127661141.45
143 rdf:type schema:Person
144 sg:person.01175774341.29 schema:affiliation https://www.grid.ac/institutes/grid.6142.1
145 schema:familyName Crowley
146 schema:givenName Peter B.
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01175774341.29
148 rdf:type schema:Person
149 sg:pub.10.1007/bf00197809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001469691
150 https://doi.org/10.1007/bf00197809
151 rdf:type schema:CreativeWork
152 sg:pub.10.1023/a:1011254402785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013814896
153 https://doi.org/10.1023/a:1011254402785
154 rdf:type schema:CreativeWork
155 sg:pub.10.1038/nchem.2107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030878022
156 https://doi.org/10.1038/nchem.2107
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/nrd1033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000722784
159 https://doi.org/10.1038/nrd1033
160 rdf:type schema:CreativeWork
161 https://app.dimensions.ai/details/publication/pub.1077283373 schema:CreativeWork
162 https://app.dimensions.ai/details/publication/pub.1083331425 schema:CreativeWork
163 https://doi.org/10.1002/anie.200462415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051738232
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1002/anie.200902672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051926603
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1002/anie.201206563 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041416479
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1002/anie.201405664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025569457
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1002/bit.21482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009525756
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1002/cbic.200700603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021870015
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1002/chem.200901410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013231151
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1002/jps.21170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036429275
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1002/pro.5560010907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004894742
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1002/prot.20449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027764068
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/b978-0-12-391927-4.10024-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052518752
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.jconrel.2011.10.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013971739
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.sbi.2010.01.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037874026
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/s0142-9612(98)00007-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038121526
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/s0969-2126(00)00065-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020923852
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1021/bc049781+ schema:sameAs https://app.dimensions.ai/details/publication/pub.1055153675
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1021/bc049843w schema:sameAs https://app.dimensions.ai/details/publication/pub.1055153712
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1021/bc2003583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050471136
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1021/bc9003052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055155858
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1021/bi800125h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055208349
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1021/bm100999a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055221766
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1021/bm300561y schema:sameAs https://app.dimensions.ai/details/publication/pub.1055222462
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1021/ja021264j schema:sameAs https://app.dimensions.ai/details/publication/pub.1055826972
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1021/ja209653n schema:sameAs https://app.dimensions.ai/details/publication/pub.1047131688
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1021/ja900786z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055860693
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1021/ja9942787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055873422
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1021/jp9058966 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015196255
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1021/ma60077a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056201934
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1021/mp200470c schema:sameAs https://app.dimensions.ai/details/publication/pub.1056212725
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1039/c3sc22015j schema:sameAs https://app.dimensions.ai/details/publication/pub.1002027352
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1056/nejm200012073432301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022881380
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.94.16.8399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040019333
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1107/s0021889807021206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012753217
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1107/s0907444902014439 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012218185
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1107/s0907444904019158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020281098
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1107/s0907444911001314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032555428
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1107/s0907444911047834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022229088
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1107/s0907444994003112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025573949
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1146/annurev.biophys.37.032807.125817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026164216
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1524/zksu.2006.suppl_23.613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067652420
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1529/biophysj.106.097717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015730911
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1529/biophysj.107.114314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006742326
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1529/biophysj.108.133025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029867983
248 rdf:type schema:CreativeWork
249 https://www.grid.ac/institutes/grid.6142.1 schema:alternateName National University of Ireland, Galway
250 schema:name School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland
251 rdf:type schema:Organization
252 https://www.grid.ac/institutes/grid.8217.c schema:alternateName Trinity College Dublin
253 schema:name School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
254 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...