Biosynthesis of the tunicamycin antibiotics proceeds via unique exo-glycal intermediates View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-05-20

AUTHORS

Filip J. Wyszynski, Seung Seo Lee, Tomoaki Yabe, Hua Wang, Juan Pablo Gomez-Escribano, Mervyn J. Bibb, Soo Jae Lee, Gideon J. Davies, Benjamin G. Davis

ABSTRACT

The tunicamycins are archetypal nucleoside antibiotics targeting bacterial peptidoglycan biosynthesis and eukaryotic protein N-glycosylation. Understanding the biosynthesis of their unusual carbon framework may lead to variants with improved selectivity. Here, we demonstrate in vitro recapitulation of key sugar-manipulating enzymes from this pathway. TunA is found to exhibit unusual regioselectivity in the reduction of a key α,β-unsaturated ketone. The product of this reaction is shown to be the preferred substrate for TunF—an epimerase that converts the glucose derivative to a galactose. In Streptomyces strains in which another gene (tunB) is deleted, the biosynthesis is shown to stall at this exo-glycal product. These investigations confirm the combined TunA/F activity and delineate the ordering of events in the metabolic pathway. This is the first time these surprising exo-glycal intermediates have been seen in biology. They suggest that construction of the aminodialdose core of tunicamycin exploits their enol ether motif in a mode of C–C bond formation not previously observed in nature, to create an 11-carbon chain. More... »

PAGES

539-546

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nchem.1351

DOI

http://dx.doi.org/10.1038/nchem.1351

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023315504

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22717438


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Bacterial Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biocatalysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbohydrate Epimerases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Computational Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydro-Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ketones", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multigene Family", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pseudomonas aeruginosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stereoisomerism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tunicamycin", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK", 
          "id": "http://www.grid.ac/institutes/grid.4991.5", 
          "name": [
            "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wyszynski", 
        "givenName": "Filip J.", 
        "id": "sg:person.01236247427.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236247427.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK", 
          "id": "http://www.grid.ac/institutes/grid.4991.5", 
          "name": [
            "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Seung Seo", 
        "id": "sg:person.01050307041.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050307041.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK", 
          "id": "http://www.grid.ac/institutes/grid.4991.5", 
          "name": [
            "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yabe", 
        "givenName": "Tomoaki", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK", 
          "id": "http://www.grid.ac/institutes/grid.4991.5", 
          "name": [
            "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Hua", 
        "id": "sg:person.011165244016.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011165244016.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gomez-Escribano", 
        "givenName": "Juan Pablo", 
        "id": "sg:person.0675205127.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675205127.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK", 
          "id": "http://www.grid.ac/institutes/grid.14830.3e", 
          "name": [
            "Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bibb", 
        "givenName": "Mervyn J.", 
        "id": "sg:person.0612653332.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612653332.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "College of Pharmacy, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk, Korea", 
          "id": "http://www.grid.ac/institutes/grid.254229.a", 
          "name": [
            "College of Pharmacy, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Soo Jae", 
        "id": "sg:person.01273442075.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273442075.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, The University of York, YO10 5DD, Heslington, York, UK", 
          "id": "http://www.grid.ac/institutes/grid.5685.e", 
          "name": [
            "Department of Chemistry, The University of York, YO10 5DD, Heslington, York, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davies", 
        "givenName": "Gideon J.", 
        "id": "sg:person.01211607401.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211607401.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK", 
          "id": "http://www.grid.ac/institutes/grid.4991.5", 
          "name": [
            "Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davis", 
        "givenName": "Benjamin G.", 
        "id": "sg:person.011632210747.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011632210747.10"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.3839/jksabc.2011.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010366378", 
          "https://doi.org/10.3839/jksabc.2011.021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/icb.1981.39", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008386260", 
          "https://doi.org/10.1038/icb.1981.39"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13238-010-0127-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027507999", 
          "https://doi.org/10.1007/s13238-010-0127-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb1203-980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040800493", 
          "https://doi.org/10.1038/nsb1203-980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro727", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007781849", 
          "https://doi.org/10.1038/nrmicro727"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-05-20", 
    "datePublishedReg": "2012-05-20", 
    "description": "The tunicamycins are archetypal nucleoside antibiotics targeting bacterial peptidoglycan biosynthesis and eukaryotic protein N-glycosylation. Understanding the biosynthesis of their unusual carbon framework may lead to variants with improved selectivity. Here, we demonstrate in vitro recapitulation of key sugar-manipulating enzymes from this pathway. TunA is found to exhibit unusual regioselectivity in the reduction of a key \u03b1,\u03b2-unsaturated ketone. The product of this reaction is shown to be the preferred substrate for TunF\u2014an epimerase that converts the glucose derivative to a galactose. In Streptomyces strains in which another gene (tunB) is deleted, the biosynthesis is shown to stall at this exo-glycal product. These investigations confirm the combined TunA/F activity and delineate the ordering of events in the metabolic pathway. This is the first time these surprising exo-glycal intermediates have been seen in biology. They suggest that construction of the aminodialdose core of tunicamycin exploits their enol ether motif in a mode of C\u2013C bond formation not previously observed in nature, to create an 11-carbon chain.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nchem.1351", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2785098", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2786146", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2759798", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2759162", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2776566", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1041224", 
        "issn": [
          "1755-4330", 
          "1755-4349"
        ], 
        "name": "Nature Chemistry", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "4"
      }
    ], 
    "keywords": [
      "eukaryotic protein N", 
      "bacterial peptidoglycan biosynthesis", 
      "peptidoglycan biosynthesis", 
      "protein N", 
      "preferred substrate", 
      "biosynthesis", 
      "metabolic pathways", 
      "nucleoside antibiotics", 
      "tunicamycin", 
      "pathway", 
      "epimerase", 
      "genes", 
      "Streptomyces", 
      "glycosylation", 
      "biology", 
      "motif", 
      "enzyme", 
      "intermediates", 
      "bond formation", 
      "first time", 
      "ordering of events", 
      "recapitulation", 
      "galactose", 
      "variants", 
      "tuna", 
      "glucose derivatives", 
      "substrate", 
      "activity", 
      "products", 
      "formation", 
      "chain", 
      "events", 
      "improved selectivity", 
      "antibiotics", 
      "proceeds", 
      "selectivity", 
      "derivatives", 
      "nature", 
      "reduction", 
      "core", 
      "investigation", 
      "reaction", 
      "unusual regioselectivity", 
      "mode", 
      "time", 
      "ketones", 
      "construction", 
      "regioselectivity", 
      "framework", 
      "carbon framework", 
      "ordering", 
      "archetypal nucleoside antibiotics", 
      "unusual carbon framework", 
      "key sugar-manipulating enzymes", 
      "sugar-manipulating enzymes", 
      "TunF", 
      "exo-glycal product", 
      "combined TunA/F activity", 
      "TunA/F activity", 
      "F activity", 
      "surprising exo-glycal intermediates", 
      "exo-glycal intermediates", 
      "aminodialdose core", 
      "enol ether motif", 
      "ether motif", 
      "unique exo-glycal intermediates"
    ], 
    "name": "Biosynthesis of the tunicamycin antibiotics proceeds via unique exo-glycal intermediates", 
    "pagination": "539-546", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023315504"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nchem.1351"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22717438"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nchem.1351", 
      "https://app.dimensions.ai/details/publication/pub.1023315504"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_575.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nchem.1351"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nchem.1351'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nchem.1351'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nchem.1351'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nchem.1351'


 

This table displays all metadata directly associated to this object as RDF triples.

270 TRIPLES      22 PREDICATES      109 URIs      97 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nchem.1351 schema:about N1e5d103a751a487ca6349c2451732453
2 N3efbb8c43fde41389b58ba4048a002c0
3 N4ff55604262a4eae968d96e3f9655fac
4 Nad609f13f5e648cf9500684f4664821a
5 Nb6d9aad4d8d449368a113a369e507ae6
6 Nc064c65c86444f789222d1625dbee0e0
7 Nc97e4721bedf4e129140b81b7b80a4fb
8 Ncad6b3fc525e4882837dad0441e36489
9 Nd468b49767b94dedaa6c403dba1157a5
10 Ndcc6a7a661094de2999a1d873cd04f8c
11 Neb4a19a89c764af58b23234b70b5ad9e
12 Nf80108f5a8da4ab9a532648528b38e36
13 Nfec37018f08841bb83264008a9eb5a6b
14 anzsrc-for:03
15 schema:author N4f816342696443f8b759f09ae621e57d
16 schema:citation sg:pub.10.1007/s13238-010-0127-6
17 sg:pub.10.1038/icb.1981.39
18 sg:pub.10.1038/nrmicro727
19 sg:pub.10.1038/nsb1203-980
20 sg:pub.10.3839/jksabc.2011.021
21 schema:datePublished 2012-05-20
22 schema:datePublishedReg 2012-05-20
23 schema:description The tunicamycins are archetypal nucleoside antibiotics targeting bacterial peptidoglycan biosynthesis and eukaryotic protein N-glycosylation. Understanding the biosynthesis of their unusual carbon framework may lead to variants with improved selectivity. Here, we demonstrate in vitro recapitulation of key sugar-manipulating enzymes from this pathway. TunA is found to exhibit unusual regioselectivity in the reduction of a key α,β-unsaturated ketone. The product of this reaction is shown to be the preferred substrate for TunF—an epimerase that converts the glucose derivative to a galactose. In Streptomyces strains in which another gene (tunB) is deleted, the biosynthesis is shown to stall at this exo-glycal product. These investigations confirm the combined TunA/F activity and delineate the ordering of events in the metabolic pathway. This is the first time these surprising exo-glycal intermediates have been seen in biology. They suggest that construction of the aminodialdose core of tunicamycin exploits their enol ether motif in a mode of C–C bond formation not previously observed in nature, to create an 11-carbon chain.
24 schema:genre article
25 schema:inLanguage en
26 schema:isAccessibleForFree false
27 schema:isPartOf N8131db5bce3743a7bb522d40cc97db30
28 N87f87ead14f24db6be5e68af4091e4bf
29 sg:journal.1041224
30 schema:keywords F activity
31 Streptomyces
32 TunA/F activity
33 TunF
34 activity
35 aminodialdose core
36 antibiotics
37 archetypal nucleoside antibiotics
38 bacterial peptidoglycan biosynthesis
39 biology
40 biosynthesis
41 bond formation
42 carbon framework
43 chain
44 combined TunA/F activity
45 construction
46 core
47 derivatives
48 enol ether motif
49 enzyme
50 epimerase
51 ether motif
52 eukaryotic protein N
53 events
54 exo-glycal intermediates
55 exo-glycal product
56 first time
57 formation
58 framework
59 galactose
60 genes
61 glucose derivatives
62 glycosylation
63 improved selectivity
64 intermediates
65 investigation
66 ketones
67 key sugar-manipulating enzymes
68 metabolic pathways
69 mode
70 motif
71 nature
72 nucleoside antibiotics
73 ordering
74 ordering of events
75 pathway
76 peptidoglycan biosynthesis
77 preferred substrate
78 proceeds
79 products
80 protein N
81 reaction
82 recapitulation
83 reduction
84 regioselectivity
85 selectivity
86 substrate
87 sugar-manipulating enzymes
88 surprising exo-glycal intermediates
89 time
90 tuna
91 tunicamycin
92 unique exo-glycal intermediates
93 unusual carbon framework
94 unusual regioselectivity
95 variants
96 schema:name Biosynthesis of the tunicamycin antibiotics proceeds via unique exo-glycal intermediates
97 schema:pagination 539-546
98 schema:productId N08d7083363a744b29a6a8c014e5fda1f
99 N39be7865e9e84b79bc5ed24caeb79bae
100 N6e36cb95c31948cd82bdeb844af37bde
101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023315504
102 https://doi.org/10.1038/nchem.1351
103 schema:sdDatePublished 2021-12-01T19:27
104 schema:sdLicense https://scigraph.springernature.com/explorer/license/
105 schema:sdPublisher Nd5aeefa8f2fd4a47b4b6946afaab1c2c
106 schema:url https://doi.org/10.1038/nchem.1351
107 sgo:license sg:explorer/license/
108 sgo:sdDataset articles
109 rdf:type schema:ScholarlyArticle
110 N08d7083363a744b29a6a8c014e5fda1f schema:name dimensions_id
111 schema:value pub.1023315504
112 rdf:type schema:PropertyValue
113 N1e5d103a751a487ca6349c2451732453 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Hydro-Lyases
115 rdf:type schema:DefinedTerm
116 N203a0197fe124ef98f4ab29706e176c0 rdf:first sg:person.011165244016.40
117 rdf:rest Nc9d6114df70442d6a4d8172930ad6320
118 N295cbb6ae01d4eb8a839b9104f4f83ab schema:affiliation grid-institutes:grid.4991.5
119 schema:familyName Yabe
120 schema:givenName Tomoaki
121 rdf:type schema:Person
122 N3003e30c21c74b17b45e67aa63fc5f01 rdf:first sg:person.01050307041.61
123 rdf:rest Ncb7dda48bb0e4c5c8784580730abc0a0
124 N35edb39203d743bf9da5b6a58afa6785 rdf:first sg:person.0612653332.06
125 rdf:rest N900a2627fdb14ad9a53806f2abb7723d
126 N39be7865e9e84b79bc5ed24caeb79bae schema:name doi
127 schema:value 10.1038/nchem.1351
128 rdf:type schema:PropertyValue
129 N3efbb8c43fde41389b58ba4048a002c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Anti-Bacterial Agents
131 rdf:type schema:DefinedTerm
132 N3fe459849f914f5fb2930d2b9b82ba0d rdf:first sg:person.011632210747.10
133 rdf:rest rdf:nil
134 N4f816342696443f8b759f09ae621e57d rdf:first sg:person.01236247427.79
135 rdf:rest N3003e30c21c74b17b45e67aa63fc5f01
136 N4ff55604262a4eae968d96e3f9655fac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Tunicamycin
138 rdf:type schema:DefinedTerm
139 N6e36cb95c31948cd82bdeb844af37bde schema:name pubmed_id
140 schema:value 22717438
141 rdf:type schema:PropertyValue
142 N8131db5bce3743a7bb522d40cc97db30 schema:volumeNumber 4
143 rdf:type schema:PublicationVolume
144 N87f87ead14f24db6be5e68af4091e4bf schema:issueNumber 7
145 rdf:type schema:PublicationIssue
146 N900a2627fdb14ad9a53806f2abb7723d rdf:first sg:person.01273442075.90
147 rdf:rest Nf564064c443248e485069f46f5943e82
148 Nad609f13f5e648cf9500684f4664821a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Biocatalysis
150 rdf:type schema:DefinedTerm
151 Nb6d9aad4d8d449368a113a369e507ae6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Stereoisomerism
153 rdf:type schema:DefinedTerm
154 Nc064c65c86444f789222d1625dbee0e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Multigene Family
156 rdf:type schema:DefinedTerm
157 Nc97e4721bedf4e129140b81b7b80a4fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Pseudomonas aeruginosa
159 rdf:type schema:DefinedTerm
160 Nc9d6114df70442d6a4d8172930ad6320 rdf:first sg:person.0675205127.90
161 rdf:rest N35edb39203d743bf9da5b6a58afa6785
162 Ncad6b3fc525e4882837dad0441e36489 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Ketones
164 rdf:type schema:DefinedTerm
165 Ncb7dda48bb0e4c5c8784580730abc0a0 rdf:first N295cbb6ae01d4eb8a839b9104f4f83ab
166 rdf:rest N203a0197fe124ef98f4ab29706e176c0
167 Nd468b49767b94dedaa6c403dba1157a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Bacterial Proteins
169 rdf:type schema:DefinedTerm
170 Nd5aeefa8f2fd4a47b4b6946afaab1c2c schema:name Springer Nature - SN SciGraph project
171 rdf:type schema:Organization
172 Ndcc6a7a661094de2999a1d873cd04f8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Computational Biology
174 rdf:type schema:DefinedTerm
175 Neb4a19a89c764af58b23234b70b5ad9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Carbohydrate Epimerases
177 rdf:type schema:DefinedTerm
178 Nf564064c443248e485069f46f5943e82 rdf:first sg:person.01211607401.09
179 rdf:rest N3fe459849f914f5fb2930d2b9b82ba0d
180 Nf80108f5a8da4ab9a532648528b38e36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Substrate Specificity
182 rdf:type schema:DefinedTerm
183 Nfec37018f08841bb83264008a9eb5a6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Protein Structure, Tertiary
185 rdf:type schema:DefinedTerm
186 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
187 schema:name Chemical Sciences
188 rdf:type schema:DefinedTerm
189 sg:grant.2759162 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.1351
190 rdf:type schema:MonetaryGrant
191 sg:grant.2759798 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.1351
192 rdf:type schema:MonetaryGrant
193 sg:grant.2776566 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.1351
194 rdf:type schema:MonetaryGrant
195 sg:grant.2785098 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.1351
196 rdf:type schema:MonetaryGrant
197 sg:grant.2786146 http://pending.schema.org/fundedItem sg:pub.10.1038/nchem.1351
198 rdf:type schema:MonetaryGrant
199 sg:journal.1041224 schema:issn 1755-4330
200 1755-4349
201 schema:name Nature Chemistry
202 schema:publisher Springer Nature
203 rdf:type schema:Periodical
204 sg:person.01050307041.61 schema:affiliation grid-institutes:grid.4991.5
205 schema:familyName Lee
206 schema:givenName Seung Seo
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050307041.61
208 rdf:type schema:Person
209 sg:person.011165244016.40 schema:affiliation grid-institutes:grid.4991.5
210 schema:familyName Wang
211 schema:givenName Hua
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011165244016.40
213 rdf:type schema:Person
214 sg:person.011632210747.10 schema:affiliation grid-institutes:grid.4991.5
215 schema:familyName Davis
216 schema:givenName Benjamin G.
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011632210747.10
218 rdf:type schema:Person
219 sg:person.01211607401.09 schema:affiliation grid-institutes:grid.5685.e
220 schema:familyName Davies
221 schema:givenName Gideon J.
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211607401.09
223 rdf:type schema:Person
224 sg:person.01236247427.79 schema:affiliation grid-institutes:grid.4991.5
225 schema:familyName Wyszynski
226 schema:givenName Filip J.
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236247427.79
228 rdf:type schema:Person
229 sg:person.01273442075.90 schema:affiliation grid-institutes:grid.254229.a
230 schema:familyName Lee
231 schema:givenName Soo Jae
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273442075.90
233 rdf:type schema:Person
234 sg:person.0612653332.06 schema:affiliation grid-institutes:grid.14830.3e
235 schema:familyName Bibb
236 schema:givenName Mervyn J.
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612653332.06
238 rdf:type schema:Person
239 sg:person.0675205127.90 schema:affiliation grid-institutes:grid.14830.3e
240 schema:familyName Gomez-Escribano
241 schema:givenName Juan Pablo
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675205127.90
243 rdf:type schema:Person
244 sg:pub.10.1007/s13238-010-0127-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027507999
245 https://doi.org/10.1007/s13238-010-0127-6
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/icb.1981.39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008386260
248 https://doi.org/10.1038/icb.1981.39
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/nrmicro727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007781849
251 https://doi.org/10.1038/nrmicro727
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/nsb1203-980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040800493
254 https://doi.org/10.1038/nsb1203-980
255 rdf:type schema:CreativeWork
256 sg:pub.10.3839/jksabc.2011.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010366378
257 https://doi.org/10.3839/jksabc.2011.021
258 rdf:type schema:CreativeWork
259 grid-institutes:grid.14830.3e schema:alternateName Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK
260 schema:name Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK
261 rdf:type schema:Organization
262 grid-institutes:grid.254229.a schema:alternateName College of Pharmacy, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk, Korea
263 schema:name College of Pharmacy, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk, Korea
264 rdf:type schema:Organization
265 grid-institutes:grid.4991.5 schema:alternateName Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK
266 schema:name Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, UK
267 rdf:type schema:Organization
268 grid-institutes:grid.5685.e schema:alternateName Department of Chemistry, The University of York, YO10 5DD, Heslington, York, UK
269 schema:name Department of Chemistry, The University of York, YO10 5DD, Heslington, York, UK
270 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...