Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-08

AUTHORS

Christoph Patsch, Ludivine Challet-Meylan, Eva C. Thoma, Eduard Urich, Tobias Heckel, John F. O’Sullivan, Stephanie J. Grainger, Friedrich G. Kapp, Lin Sun, Klaus Christensen, Yulei Xia, Mary H. C. Florido, Wei He, Wei Pan, Michael Prummer, Curtis R. Warren, Roland Jakob-Roetne, Ulrich Certa, Ravi Jagasia, Per-Ola Freskgård, Isaac Adatto, Dorothee Kling, Paul Huang, Leonard I. Zon, Elliot L. Chaikof, Robert E. Gerszten, Martin Graf, Roberto Iacone, Chad A. Cowan

ABSTRACT

The use of human pluripotent stem cells for in vitro disease modelling and clinical applications requires protocols that convert these cells into relevant adult cell types. Here, we report the rapid and efficient differentiation of human pluripotent stem cells into vascular endothelial and smooth muscle cells. We found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal fate and subsequent exposure to VEGF-A or PDGF-BB resulted in the differentiation of either endothelial or vascular smooth muscle cells, respectively. Both protocols produced mature cells with efficiencies exceeding 80% within six days. On purification to 99% via surface markers, endothelial cells maintained their identity, as assessed by marker gene expression, and showed relevant in vitro and in vivo functionality. Global transcriptional and metabolomic analyses confirmed that the cells closely resembled their in vivo counterparts. Our results suggest that these cells could be used to faithfully model human disease. More... »

PAGES

994-1003

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncb3205

    DOI

    http://dx.doi.org/10.1038/ncb3205

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018887805

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26214132


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