Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-08

AUTHORS

Christoph Patsch, Ludivine Challet-Meylan, Eva C. Thoma, Eduard Urich, Tobias Heckel, John F. O’Sullivan, Stephanie J. Grainger, Friedrich G. Kapp, Lin Sun, Klaus Christensen, Yulei Xia, Mary H. C. Florido, Wei He, Wei Pan, Michael Prummer, Curtis R. Warren, Roland Jakob-Roetne, Ulrich Certa, Ravi Jagasia, Per-Ola Freskgård, Isaac Adatto, Dorothee Kling, Paul Huang, Leonard I. Zon, Elliot L. Chaikof, Robert E. Gerszten, Martin Graf, Roberto Iacone, Chad A. Cowan

ABSTRACT

The use of human pluripotent stem cells for in vitro disease modelling and clinical applications requires protocols that convert these cells into relevant adult cell types. Here, we report the rapid and efficient differentiation of human pluripotent stem cells into vascular endothelial and smooth muscle cells. We found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal fate and subsequent exposure to VEGF-A or PDGF-BB resulted in the differentiation of either endothelial or vascular smooth muscle cells, respectively. Both protocols produced mature cells with efficiencies exceeding 80% within six days. On purification to 99% via surface markers, endothelial cells maintained their identity, as assessed by marker gene expression, and showed relevant in vitro and in vivo functionality. Global transcriptional and metabolomic analyses confirmed that the cells closely resembled their in vivo counterparts. Our results suggest that these cells could be used to faithfully model human disease. More... »

PAGES

994-1003

References to SciGraph publications

  • 2000-09. Atherosclerosis in NATURE
  • 1977-11. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture in NATURE
  • 2000-11. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors in NATURE
  • 2011-04. Investigating monogenic and complex diseases with pluripotent stem cells in NATURE REVIEWS GENETICS
  • 2007-06. A ROCK inhibitor permits survival of dissociated human embryonic stem cells in NATURE BIOTECHNOLOGY
  • 2015-08-06. Endothelial cells differentiation from hPSCs in PROTOCOL EXCHANGE
  • 2010-02. Expansion and maintenance of human embryonic stem cell–derived endothelial cells by TGFβ inhibition is Id1 dependent in NATURE BIOTECHNOLOGY
  • 2011-12. Diseases in a dish: modeling human genetic disorders using induced pluripotent cells in NATURE MEDICINE
  • 2007-05. Gene function in mouse embryogenesis: get set for gastrulation in NATURE REVIEWS GENETICS
  • 2004-01. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor in NATURE MEDICINE
  • 2010-04. The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2011-11-11. Induced pluripotent stem cells — opportunities for disease modelling and drug discovery in NATURE REVIEWS DRUG DISCOVERY
  • 2007-03. Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo in NATURE BIOTECHNOLOGY
  • 2011-04. Metabolite profiles and the risk of developing diabetes in NATURE MEDICINE
  • 2012-02. Generation of human vascular smooth muscle subtypes provides insight into embryological origin–dependent disease susceptibility in NATURE BIOTECHNOLOGY
  • 2015-08-07. Vascular smooth muscle cells differentiation from hPSCs in PROTOCOL EXCHANGE
  • 2005-03. A small molecule–kinase interaction map for clinical kinase inhibitors in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncb3205

    DOI

    http://dx.doi.org/10.1038/ncb3205

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018887805

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26214132


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