Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-03

AUTHORS

Jem A. Efe, Simon Hilcove, Janghwan Kim, Hongyan Zhou, Kunfu Ouyang, Gang Wang, Ju Chen, Sheng Ding

ABSTRACT

Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously contracting patches of differentiated cardiomyocytes over a period of 11-12 days. Several lines of evidence suggest that a pluripotent intermediate is not involved. Our method represents a unique strategy that allows a transient, plastic developmental state established early in reprogramming to effectively function as a cellular transdifferentiation platform, the use of which could extend beyond cardiogenesis. Our study has potentially wide-ranging implications for induced pluripotent stem cell (iPSC)-factor-based reprogramming and broadens the existing paradigm. More... »

PAGES

215

Journal

TITLE

Nature Cell Biology

ISSUE

3

VOLUME

13

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncb2164

DOI

http://dx.doi.org/10.1038/ncb2164

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034013325

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21278734


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophysiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Induced Pluripotent Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocytes, Cardiac", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Retroviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Efe", 
        "givenName": "Jem A.", 
        "id": "sg:person.0605775667.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605775667.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hilcove", 
        "givenName": "Simon", 
        "id": "sg:person.01106230526.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106230526.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Janghwan", 
        "id": "sg:person.0576205757.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576205757.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Hongyan", 
        "id": "sg:person.010655102477.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010655102477.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Diego", 
          "id": "https://www.grid.ac/institutes/grid.266100.3", 
          "name": [
            "Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ouyang", 
        "givenName": "Kunfu", 
        "id": "sg:person.01263673015.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263673015.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Diego", 
          "id": "https://www.grid.ac/institutes/grid.266100.3", 
          "name": [
            "Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Gang", 
        "id": "sg:person.01251072026.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251072026.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Diego", 
          "id": "https://www.grid.ac/institutes/grid.266100.3", 
          "name": [
            "Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Ju", 
        "id": "sg:person.013345564407.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013345564407.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Scripps Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.214007.0", 
          "name": [
            "Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ding", 
        "givenName": "Sheng", 
        "id": "sg:person.0661414626.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661414626.09"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nbt1374", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002744128", 
          "https://doi.org/10.1038/nbt1374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2007.418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003600573", 
          "https://doi.org/10.1038/nprot.2007.418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004160248", 
          "https://doi.org/10.1038/nbt1483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.03.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004352286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2008.03.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006084359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.08-128546", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006185192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2009.04.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006373679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2005.09.051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007624851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2008.02.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008490443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c500026200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009561095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2007.08.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011219533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012799593", 
          "https://doi.org/10.1038/nbt.1520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2007.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012875023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.2434235100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014147833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014946219", 
          "https://doi.org/10.1038/ncb1696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2007.05.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016119169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00246-008-9352-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016865449", 
          "https://doi.org/10.1007/s00246-008-9352-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00246-008-9352-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016865449", 
          "https://doi.org/10.1007/s00246-008-9352-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.016865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017243342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.20191", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017953925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07863", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019816783", 
          "https://doi.org/10.1038/nature07863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.020867", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021026862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021108695", 
          "https://doi.org/10.1038/nmeth.1409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021108695", 
          "https://doi.org/10.1038/nmeth.1409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.07.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022964788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/cvr/cvq111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024660839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.101063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028987754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030291386", 
          "https://doi.org/10.1038/nature08592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030291386", 
          "https://doi.org/10.1038/nature08592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pcbi.1000785", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030854248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2006-06-030551", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034248358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.01832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035030956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2008-0033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039147822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2010.04.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042237086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1741-7007-8-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042606041", 
          "https://doi.org/10.1186/1741-7007-8-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2005.10.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042970110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.07.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052718634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2008.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053248333"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-03", 
    "datePublishedReg": "2011-03-01", 
    "description": "Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously contracting patches of differentiated cardiomyocytes over a period of 11-12 days. Several lines of evidence suggest that a pluripotent intermediate is not involved. Our method represents a unique strategy that allows a transient, plastic developmental state established early in reprogramming to effectively function as a cellular transdifferentiation platform, the use of which could extend beyond cardiogenesis. Our study has potentially wide-ranging implications for induced pluripotent stem cell (iPSC)-factor-based reprogramming and broadens the existing paradigm.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/ncb2164", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1021344", 
        "issn": [
          "1465-7392", 
          "1476-4679"
        ], 
        "name": "Nature Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "name": "Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy", 
    "pagination": "215", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a2084baf8c5858d4b4356c9dfc10c517bd70b33ea487a1bf3b0a7db033e6ddaa"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21278734"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100890575"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ncb2164"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034013325"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ncb2164", 
      "https://app.dimensions.ai/details/publication/pub.1034013325"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:46", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000435.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/ncb2164"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'


 

This table displays all metadata directly associated to this object as RDF triples.

299 TRIPLES      21 PREDICATES      80 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ncb2164 schema:about N1711f533733f43a19cb9b5ed93158f15
2 N23bdecff0da04eab9a91aa2425e6411b
3 N2936a2d99b2f43bca29c34340cd07d78
4 N3342bbbad89c4c91b7703d2bbf7b1d46
5 N3a1aa3750a814f56b364bd403d7296c0
6 N3d293e1cc3b7472fbec2a093626f6e2e
7 N4633ae98cbe14d3389961e68d41e7879
8 N4c67cdb28d6c45a3b96a7587ada44a7c
9 N4dd58712fd6245bda61afcaccea0a35e
10 N52d886c355cc42afa7e179594e1ad5b3
11 N592804d453084dfda1517e5bea51d2af
12 N7e812793e53747b299cd76ecf0b7622f
13 N8d00f0c3f0234fc496ed070d3903d4ad
14 N9c886bffb3d642e2a66c9c16a9200846
15 Nce08a56982fa48bdaedfb1d0b460f39c
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N706b1d184bcc42368c10e975fe0413ac
19 schema:citation sg:pub.10.1007/s00246-008-9352-7
20 sg:pub.10.1038/nature07863
21 sg:pub.10.1038/nature08592
22 sg:pub.10.1038/nbt.1520
23 sg:pub.10.1038/nbt1374
24 sg:pub.10.1038/nbt1483
25 sg:pub.10.1038/ncb1696
26 sg:pub.10.1038/nmeth.1409
27 sg:pub.10.1038/nprot.2007.418
28 sg:pub.10.1186/1741-7007-8-5
29 https://doi.org/10.1002/jcb.20191
30 https://doi.org/10.1016/j.bbagen.2010.04.004
31 https://doi.org/10.1016/j.cell.2006.07.024
32 https://doi.org/10.1016/j.cell.2008.03.028
33 https://doi.org/10.1016/j.cell.2009.07.039
34 https://doi.org/10.1016/j.cell.2010.07.002
35 https://doi.org/10.1016/j.devcel.2005.10.017
36 https://doi.org/10.1016/j.stem.2007.05.018
37 https://doi.org/10.1016/j.stem.2007.08.008
38 https://doi.org/10.1016/j.stem.2007.12.001
39 https://doi.org/10.1016/j.stem.2008.01.004
40 https://doi.org/10.1016/j.stem.2008.02.001
41 https://doi.org/10.1016/j.stem.2008.03.010
42 https://doi.org/10.1016/j.stem.2009.04.005
43 https://doi.org/10.1016/j.ydbio.2005.09.051
44 https://doi.org/10.1073/pnas.2434235100
45 https://doi.org/10.1074/jbc.c500026200
46 https://doi.org/10.1074/jbc.m110.101063
47 https://doi.org/10.1093/cvr/cvq111
48 https://doi.org/10.1096/fj.08-128546
49 https://doi.org/10.1182/blood-2006-06-030551
50 https://doi.org/10.1242/dev.016865
51 https://doi.org/10.1242/dev.01832
52 https://doi.org/10.1242/dev.020867
53 https://doi.org/10.1371/journal.pcbi.1000785
54 https://doi.org/10.1634/stemcells.2008-0033
55 schema:datePublished 2011-03
56 schema:datePublishedReg 2011-03-01
57 schema:description Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously contracting patches of differentiated cardiomyocytes over a period of 11-12 days. Several lines of evidence suggest that a pluripotent intermediate is not involved. Our method represents a unique strategy that allows a transient, plastic developmental state established early in reprogramming to effectively function as a cellular transdifferentiation platform, the use of which could extend beyond cardiogenesis. Our study has potentially wide-ranging implications for induced pluripotent stem cell (iPSC)-factor-based reprogramming and broadens the existing paradigm.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf Nd0ccd150eb0d4c3d82ef8e544455cb56
62 Ne3c2d7c2f6a446bc96a8ba94930110e5
63 sg:journal.1021344
64 schema:name Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy
65 schema:pagination 215
66 schema:productId N5747da7941854e73b9c29db35fe8bdd2
67 Nc6ad00bee1744907bffbd745ce5bf97f
68 Ne3dcfb9d77e74b089e1584473d093a85
69 Nf08b18e573eb4572bf312b52fa306bcd
70 Nf41dad7befcc41139fc327502eda6234
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034013325
72 https://doi.org/10.1038/ncb2164
73 schema:sdDatePublished 2019-04-10T19:46
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Na7958c24335249218ae2e4b1289ad6d7
76 schema:url https://www.nature.com/articles/ncb2164
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N02df15a422014cf38c4b7f037d93bd14 rdf:first sg:person.0661414626.09
81 rdf:rest rdf:nil
82 N081b46ca3939447f98f6cf2084568892 rdf:first sg:person.010655102477.06
83 rdf:rest Nd18801d32c0c404aaa33cf4536859e43
84 N1711f533733f43a19cb9b5ed93158f15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Cell Differentiation
86 rdf:type schema:DefinedTerm
87 N1fd1433836634ae4bb554e7d3e24364c rdf:first sg:person.0576205757.32
88 rdf:rest N081b46ca3939447f98f6cf2084568892
89 N23bdecff0da04eab9a91aa2425e6411b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Cell Proliferation
91 rdf:type schema:DefinedTerm
92 N2936a2d99b2f43bca29c34340cd07d78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Calcium
94 rdf:type schema:DefinedTerm
95 N3342bbbad89c4c91b7703d2bbf7b1d46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Fibroblasts
97 rdf:type schema:DefinedTerm
98 N3a1aa3750a814f56b364bd403d7296c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Myocytes, Cardiac
100 rdf:type schema:DefinedTerm
101 N3d293e1cc3b7472fbec2a093626f6e2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Flow Cytometry
103 rdf:type schema:DefinedTerm
104 N418e59a00eed4af2921a652bda4a3531 rdf:first sg:person.01106230526.99
105 rdf:rest N1fd1433836634ae4bb554e7d3e24364c
106 N4633ae98cbe14d3389961e68d41e7879 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Microscopy, Fluorescence
108 rdf:type schema:DefinedTerm
109 N4c67cdb28d6c45a3b96a7587ada44a7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Retroviridae
111 rdf:type schema:DefinedTerm
112 N4dd58712fd6245bda61afcaccea0a35e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Transgenes
114 rdf:type schema:DefinedTerm
115 N52d886c355cc42afa7e179594e1ad5b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Animals
117 rdf:type schema:DefinedTerm
118 N5747da7941854e73b9c29db35fe8bdd2 schema:name pubmed_id
119 schema:value 21278734
120 rdf:type schema:PropertyValue
121 N592804d453084dfda1517e5bea51d2af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Cell Culture Techniques
123 rdf:type schema:DefinedTerm
124 N706b1d184bcc42368c10e975fe0413ac rdf:first sg:person.0605775667.84
125 rdf:rest N418e59a00eed4af2921a652bda4a3531
126 N7e812793e53747b299cd76ecf0b7622f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Induced Pluripotent Stem Cells
128 rdf:type schema:DefinedTerm
129 N8d00f0c3f0234fc496ed070d3903d4ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Mice
131 rdf:type schema:DefinedTerm
132 N9c886bffb3d642e2a66c9c16a9200846 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Electrophysiology
134 rdf:type schema:DefinedTerm
135 Na7958c24335249218ae2e4b1289ad6d7 schema:name Springer Nature - SN SciGraph project
136 rdf:type schema:Organization
137 Nc46bd70bf544449eb9b7afce4a20e825 rdf:first sg:person.013345564407.74
138 rdf:rest N02df15a422014cf38c4b7f037d93bd14
139 Nc6ad00bee1744907bffbd745ce5bf97f schema:name nlm_unique_id
140 schema:value 100890575
141 rdf:type schema:PropertyValue
142 Nce08a56982fa48bdaedfb1d0b460f39c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Time Factors
144 rdf:type schema:DefinedTerm
145 Nd0ccd150eb0d4c3d82ef8e544455cb56 schema:issueNumber 3
146 rdf:type schema:PublicationIssue
147 Nd18801d32c0c404aaa33cf4536859e43 rdf:first sg:person.01263673015.35
148 rdf:rest Nf9734fd5cc354c33be1c847b05a2497c
149 Ne3c2d7c2f6a446bc96a8ba94930110e5 schema:volumeNumber 13
150 rdf:type schema:PublicationVolume
151 Ne3dcfb9d77e74b089e1584473d093a85 schema:name readcube_id
152 schema:value a2084baf8c5858d4b4356c9dfc10c517bd70b33ea487a1bf3b0a7db033e6ddaa
153 rdf:type schema:PropertyValue
154 Nf08b18e573eb4572bf312b52fa306bcd schema:name doi
155 schema:value 10.1038/ncb2164
156 rdf:type schema:PropertyValue
157 Nf41dad7befcc41139fc327502eda6234 schema:name dimensions_id
158 schema:value pub.1034013325
159 rdf:type schema:PropertyValue
160 Nf9734fd5cc354c33be1c847b05a2497c rdf:first sg:person.01251072026.36
161 rdf:rest Nc46bd70bf544449eb9b7afce4a20e825
162 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
163 schema:name Biological Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
166 schema:name Biochemistry and Cell Biology
167 rdf:type schema:DefinedTerm
168 sg:journal.1021344 schema:issn 1465-7392
169 1476-4679
170 schema:name Nature Cell Biology
171 rdf:type schema:Periodical
172 sg:person.010655102477.06 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
173 schema:familyName Zhou
174 schema:givenName Hongyan
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010655102477.06
176 rdf:type schema:Person
177 sg:person.01106230526.99 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
178 schema:familyName Hilcove
179 schema:givenName Simon
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106230526.99
181 rdf:type schema:Person
182 sg:person.01251072026.36 schema:affiliation https://www.grid.ac/institutes/grid.266100.3
183 schema:familyName Wang
184 schema:givenName Gang
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251072026.36
186 rdf:type schema:Person
187 sg:person.01263673015.35 schema:affiliation https://www.grid.ac/institutes/grid.266100.3
188 schema:familyName Ouyang
189 schema:givenName Kunfu
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263673015.35
191 rdf:type schema:Person
192 sg:person.013345564407.74 schema:affiliation https://www.grid.ac/institutes/grid.266100.3
193 schema:familyName Chen
194 schema:givenName Ju
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013345564407.74
196 rdf:type schema:Person
197 sg:person.0576205757.32 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
198 schema:familyName Kim
199 schema:givenName Janghwan
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576205757.32
201 rdf:type schema:Person
202 sg:person.0605775667.84 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
203 schema:familyName Efe
204 schema:givenName Jem A.
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605775667.84
206 rdf:type schema:Person
207 sg:person.0661414626.09 schema:affiliation https://www.grid.ac/institutes/grid.214007.0
208 schema:familyName Ding
209 schema:givenName Sheng
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661414626.09
211 rdf:type schema:Person
212 sg:pub.10.1007/s00246-008-9352-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016865449
213 https://doi.org/10.1007/s00246-008-9352-7
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/nature07863 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019816783
216 https://doi.org/10.1038/nature07863
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nature08592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030291386
219 https://doi.org/10.1038/nature08592
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nbt.1520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012799593
222 https://doi.org/10.1038/nbt.1520
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nbt1374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002744128
225 https://doi.org/10.1038/nbt1374
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nbt1483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004160248
228 https://doi.org/10.1038/nbt1483
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/ncb1696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014946219
231 https://doi.org/10.1038/ncb1696
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/nmeth.1409 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021108695
234 https://doi.org/10.1038/nmeth.1409
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/nprot.2007.418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003600573
237 https://doi.org/10.1038/nprot.2007.418
238 rdf:type schema:CreativeWork
239 sg:pub.10.1186/1741-7007-8-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042606041
240 https://doi.org/10.1186/1741-7007-8-5
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1002/jcb.20191 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017953925
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.bbagen.2010.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042237086
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.cell.2008.03.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004352286
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.cell.2009.07.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052718634
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.cell.2010.07.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022964788
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/j.devcel.2005.10.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042970110
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/j.stem.2007.05.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016119169
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.stem.2007.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011219533
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/j.stem.2007.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012875023
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1016/j.stem.2008.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053248333
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1016/j.stem.2008.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008490443
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/j.stem.2008.03.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006084359
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/j.stem.2009.04.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006373679
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/j.ydbio.2005.09.051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007624851
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1073/pnas.2434235100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014147833
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1074/jbc.c500026200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009561095
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1074/jbc.m110.101063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028987754
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1093/cvr/cvq111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024660839
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1096/fj.08-128546 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006185192
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1182/blood-2006-06-030551 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034248358
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1242/dev.016865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017243342
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1242/dev.01832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035030956
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1242/dev.020867 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021026862
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1371/journal.pcbi.1000785 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030854248
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1634/stemcells.2008-0033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039147822
293 rdf:type schema:CreativeWork
294 https://www.grid.ac/institutes/grid.214007.0 schema:alternateName Scripps Research Institute
295 schema:name Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
296 rdf:type schema:Organization
297 https://www.grid.ac/institutes/grid.266100.3 schema:alternateName University of California, San Diego
298 schema:name Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA.
299 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...