Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-03

AUTHORS

Jem A. Efe, Simon Hilcove, Janghwan Kim, Hongyan Zhou, Kunfu Ouyang, Gang Wang, Ju Chen, Sheng Ding

ABSTRACT

Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously contracting patches of differentiated cardiomyocytes over a period of 11-12 days. Several lines of evidence suggest that a pluripotent intermediate is not involved. Our method represents a unique strategy that allows a transient, plastic developmental state established early in reprogramming to effectively function as a cellular transdifferentiation platform, the use of which could extend beyond cardiogenesis. Our study has potentially wide-ranging implications for induced pluripotent stem cell (iPSC)-factor-based reprogramming and broadens the existing paradigm. More... »

PAGES

215

References to SciGraph publications

Journal

TITLE

Nature Cell Biology

ISSUE

3

VOLUME

13

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncb2164

    DOI

    http://dx.doi.org/10.1038/ncb2164

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034013325

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21278734


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    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncb2164'


     

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