The CENP-H–I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-05

AUTHORS

Masahiro Okada, Iain M. Cheeseman, Tetsuya Hori, Katsuya Okawa, Ian X. McLeod, John R. Yates, Arshad Desai, Tatsuo Fukagawa

ABSTRACT

In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. Here, we isolated a multi-subunit complex, which includes the established inner kinetochore components CENP-H and CENP-I, and nine other proteins, from both human and chicken cells. Our analysis of these proteins demonstrates that the CENP-H-I complex can be divided into three functional sub-complexes, each of which is required for faithful chromosome segregation. Interestingly, newly expressed CENP-A is not efficiently incorporated into centromeres in knockout mutants of a subclass of CENP-H-I complex proteins, indicating that the CENP-H-I complex may function, in part, as a marker directing CENP-A deposition to centromeres. More... »

PAGES

446-457

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncb1396

DOI

http://dx.doi.org/10.1038/ncb1396

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007269827

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16622420


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