The NuRD component Mbd3 is required for pluripotency of embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-03

AUTHORS

Keisuke Kaji, Isabel Martín Caballero, Ruth MacLeod, Jennifer Nichols, Valerie A. Wilson, Brian Hendrich

ABSTRACT

Cells of early mammalian embryos have the potential to develop into any adult cell type, and are thus said to be pluripotent. Pluripotency is lost during embryogenesis as cells commit to specific developmental pathways. Although restriction of developmental potential is often associated with repression of inappropriate genetic programmes, the role of epigenetic silencing during early lineage commitment remains undefined. Here, we used mouse embryonic stem cells to study the function of epigenetic silencing in pluripotent cells. Embryonic stem cells lacking Mbd3 - a component of the nucleosome remodelling and histone deacetylation (NuRD) complex - were viable but failed to completely silence genes that are expressed before implantation of the embryo. Mbd3-deficient embryonic stem cells could be maintained in the absence of leukaemia inhibitory factor (LIF) and could initiate differentiation in embryoid bodies or chimeric embryos, but failed to commit to developmental lineages. Our findings define a role for epigenetic silencing in the cell-fate commitment of pluripotent cells. More... »

PAGES

285-292

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncb1372

DOI

http://dx.doi.org/10.1038/ncb1372

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009099013

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16462733


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Lineage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Mammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epigenesis, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Silencing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histone Deacetylases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-6", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukemia Inhibitory Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mi-2 Nucleosome Remodeling and Deacetylase Complex", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pluripotent Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kaji", 
        "givenName": "Keisuke", 
        "id": "sg:person.01350502174.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350502174.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Caballero", 
        "givenName": "Isabel Mart\u00edn", 
        "id": "sg:person.0654621540.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654621540.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "MacLeod", 
        "givenName": "Ruth", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nichols", 
        "givenName": "Jennifer", 
        "id": "sg:person.07725017617.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07725017617.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wilson", 
        "givenName": "Valerie A.", 
        "id": "sg:person.0752707205.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752707205.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Edinburgh", 
          "id": "https://www.grid.ac/institutes/grid.4305.2", 
          "name": [
            "Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hendrich", 
        "givenName": "Brian", 
        "id": "sg:person.01355477547.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355477547.03"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/343657a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001498814", 
          "https://doi.org/10.1038/343657a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.07.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002417372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.07.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002417372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00392-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004929245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)00083-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005164056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m203455200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005518002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(98)00056-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005744835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.22.5.1526-1536.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006838536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0952-7915(99)00065-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009410516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(03)00080-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011684230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(03)00080-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011684230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012360268", 
          "https://doi.org/10.1038/nature00927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012360268", 
          "https://doi.org/10.1038/nature00927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.15.1924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012363514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.18.11.6538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013470999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(02)00002-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013502294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.167.11.6073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022803416"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.01415", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026113218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026225001", 
          "https://doi.org/10.1007/bf01666137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026225001", 
          "https://doi.org/10.1007/bf01666137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.00366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029438813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1996.0165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029442546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.12.13.2048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033063759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.00893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033869742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.194101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038747920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/12664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039527067", 
          "https://doi.org/10.1038/12664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/12664", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039527067", 
          "https://doi.org/10.1038/12664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.8.3566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040735545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.72.4.1441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040962276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.24.20.8862-8871.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041763929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042028812", 
          "https://doi.org/10.1038/nature01646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042028812", 
          "https://doi.org/10.1038/nature01646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046397624", 
          "https://doi.org/10.1038/nbt729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046397624", 
          "https://doi.org/10.1038/nbt729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050743069", 
          "https://doi.org/10.1038/nature729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050743069", 
          "https://doi.org/10.1038/nature729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.14.11.1577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060111312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.282.5396.2072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062563523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077500611", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079089305", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080020802", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.99-0810com", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100513376"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-03", 
    "datePublishedReg": "2006-03-01", 
    "description": "Cells of early mammalian embryos have the potential to develop into any adult cell type, and are thus said to be pluripotent. Pluripotency is lost during embryogenesis as cells commit to specific developmental pathways. Although restriction of developmental potential is often associated with repression of inappropriate genetic programmes, the role of epigenetic silencing during early lineage commitment remains undefined. Here, we used mouse embryonic stem cells to study the function of epigenetic silencing in pluripotent cells. Embryonic stem cells lacking Mbd3 - a component of the nucleosome remodelling and histone deacetylation (NuRD) complex - were viable but failed to completely silence genes that are expressed before implantation of the embryo. Mbd3-deficient embryonic stem cells could be maintained in the absence of leukaemia inhibitory factor (LIF) and could initiate differentiation in embryoid bodies or chimeric embryos, but failed to commit to developmental lineages. Our findings define a role for epigenetic silencing in the cell-fate commitment of pluripotent cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/ncb1372", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2766191", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3632311", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2758772", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3632816", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1021344", 
        "issn": [
          "1465-7392", 
          "1476-4679"
        ], 
        "name": "Nature Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "The NuRD component Mbd3 is required for pluripotency of embryonic stem cells", 
    "pagination": "285-292", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "2b5969571bbee67c1309bb859ffc1d1ea6dac80b4f0c154eff9d6ad6452586e8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16462733"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100890575"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ncb1372"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009099013"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ncb1372", 
      "https://app.dimensions.ai/details/publication/pub.1009099013"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87106_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/ncb1372"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncb1372'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncb1372'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncb1372'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncb1372'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      79 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ncb1372 schema:about N147d35c0233945ec8a5891e820e6e94b
2 N195515e21ed5477ab7b35c5014c9fb9d
3 N20e0d44561b041be867008dd8cc55aaa
4 N3a5e3a18dfde4e5d8db57ac41a5235bc
5 N3a63542f115049348115235c4df3ca1f
6 N4fbb6387ef254c91b79468b7b548aeec
7 N6057a583efcf4760816843b6f46b970b
8 N7a3bdb0c9a424d70a9fcfe6bc9b6abcf
9 N81556be908d2427297b0453b67e79406
10 N91425c61ab8740afb8846470397c98bf
11 N91a435d0dce3437d8e06905b93bcd7a2
12 Nbe66ef4c890641708885e7390ce56be3
13 Ncf64c9c9f3d24cf1838002ede8cf4d91
14 Nea6275620b7d4d83a852c6153945798f
15 Nee033751192b4c22aa96018c8d501615
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author N868e86257561469295f2d3099278de86
19 schema:citation sg:pub.10.1007/bf01666137
20 sg:pub.10.1038/12664
21 sg:pub.10.1038/343657a0
22 sg:pub.10.1038/nature00927
23 sg:pub.10.1038/nature01646
24 sg:pub.10.1038/nature729
25 sg:pub.10.1038/nbt729
26 sg:pub.10.1038/nbt780
27 https://app.dimensions.ai/details/publication/pub.1077500611
28 https://app.dimensions.ai/details/publication/pub.1079089305
29 https://app.dimensions.ai/details/publication/pub.1080020802
30 https://doi.org/10.1006/dbio.1996.0165
31 https://doi.org/10.1016/j.cell.2005.07.003
32 https://doi.org/10.1016/s0092-8674(03)00392-1
33 https://doi.org/10.1016/s0168-9525(03)00080-5
34 https://doi.org/10.1016/s0896-6273(00)00083-0
35 https://doi.org/10.1016/s0925-4773(02)00002-3
36 https://doi.org/10.1016/s0925-4773(98)00056-2
37 https://doi.org/10.1016/s0952-7915(99)00065-5
38 https://doi.org/10.1073/pnas.72.4.1441
39 https://doi.org/10.1073/pnas.92.8.3566
40 https://doi.org/10.1074/jbc.m203455200
41 https://doi.org/10.1096/fj.14.11.1577
42 https://doi.org/10.1096/fj.99-0810com
43 https://doi.org/10.1101/gad.12.13.2048
44 https://doi.org/10.1101/gad.13.15.1924
45 https://doi.org/10.1101/gad.194101
46 https://doi.org/10.1126/science.282.5396.2072
47 https://doi.org/10.1128/mcb.18.11.6538
48 https://doi.org/10.1128/mcb.22.5.1526-1536.2002
49 https://doi.org/10.1128/mcb.24.20.8862-8871.2004
50 https://doi.org/10.1242/dev.00366
51 https://doi.org/10.1242/dev.00893
52 https://doi.org/10.1242/dev.01415
53 https://doi.org/10.4049/jimmunol.167.11.6073
54 schema:datePublished 2006-03
55 schema:datePublishedReg 2006-03-01
56 schema:description Cells of early mammalian embryos have the potential to develop into any adult cell type, and are thus said to be pluripotent. Pluripotency is lost during embryogenesis as cells commit to specific developmental pathways. Although restriction of developmental potential is often associated with repression of inappropriate genetic programmes, the role of epigenetic silencing during early lineage commitment remains undefined. Here, we used mouse embryonic stem cells to study the function of epigenetic silencing in pluripotent cells. Embryonic stem cells lacking Mbd3 - a component of the nucleosome remodelling and histone deacetylation (NuRD) complex - were viable but failed to completely silence genes that are expressed before implantation of the embryo. Mbd3-deficient embryonic stem cells could be maintained in the absence of leukaemia inhibitory factor (LIF) and could initiate differentiation in embryoid bodies or chimeric embryos, but failed to commit to developmental lineages. Our findings define a role for epigenetic silencing in the cell-fate commitment of pluripotent cells.
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree false
60 schema:isPartOf N97563a7408a240f7845a762b49635121
61 Nd3ea27aa38eb4ef29c10fc79b9c8ccff
62 sg:journal.1021344
63 schema:name The NuRD component Mbd3 is required for pluripotency of embryonic stem cells
64 schema:pagination 285-292
65 schema:productId N4221607a06da41338a4d4b0d63ec512a
66 N427e3463c90d447f829fa9f62ec3de67
67 N4fc075cecf074642bdfdd8858642c20a
68 N655028c8850c4b42bab23d046380afb6
69 Nefaa140b48f34458ad585001f25707e8
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009099013
71 https://doi.org/10.1038/ncb1372
72 schema:sdDatePublished 2019-04-11T12:25
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N53f85715c0d2414ea457bd4ec1761cea
75 schema:url http://www.nature.com/articles/ncb1372
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N0614c92ea98843bdb73d3b86818ee750 rdf:first sg:person.01355477547.03
80 rdf:rest rdf:nil
81 N147d35c0233945ec8a5891e820e6e94b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Embryo, Mammalian
83 rdf:type schema:DefinedTerm
84 N195515e21ed5477ab7b35c5014c9fb9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Cell Lineage
86 rdf:type schema:DefinedTerm
87 N20e0d44561b041be867008dd8cc55aaa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Cells, Cultured
89 rdf:type schema:DefinedTerm
90 N28d664bf3f7049459de75ee0c426cbb9 rdf:first sg:person.07725017617.42
91 rdf:rest Nb743d4bfd81e470aa596510217a2edff
92 N3a5e3a18dfde4e5d8db57ac41a5235bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Interleukin-6
94 rdf:type schema:DefinedTerm
95 N3a63542f115049348115235c4df3ca1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Leukemia Inhibitory Factor
97 rdf:type schema:DefinedTerm
98 N4221607a06da41338a4d4b0d63ec512a schema:name doi
99 schema:value 10.1038/ncb1372
100 rdf:type schema:PropertyValue
101 N427e3463c90d447f829fa9f62ec3de67 schema:name nlm_unique_id
102 schema:value 100890575
103 rdf:type schema:PropertyValue
104 N4fbb6387ef254c91b79468b7b548aeec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Histone Deacetylases
106 rdf:type schema:DefinedTerm
107 N4fc075cecf074642bdfdd8858642c20a schema:name readcube_id
108 schema:value 2b5969571bbee67c1309bb859ffc1d1ea6dac80b4f0c154eff9d6ad6452586e8
109 rdf:type schema:PropertyValue
110 N53f85715c0d2414ea457bd4ec1761cea schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 N6057a583efcf4760816843b6f46b970b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Gene Silencing
114 rdf:type schema:DefinedTerm
115 N655028c8850c4b42bab23d046380afb6 schema:name dimensions_id
116 schema:value pub.1009099013
117 rdf:type schema:PropertyValue
118 N7a3bdb0c9a424d70a9fcfe6bc9b6abcf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Transcription Factors
120 rdf:type schema:DefinedTerm
121 N81556be908d2427297b0453b67e79406 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Cell Differentiation
123 rdf:type schema:DefinedTerm
124 N868e86257561469295f2d3099278de86 rdf:first sg:person.01350502174.86
125 rdf:rest Nf0f7609eab09420c8141d7c505327545
126 N91425c61ab8740afb8846470397c98bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Mi-2 Nucleosome Remodeling and Deacetylase Complex
128 rdf:type schema:DefinedTerm
129 N91a435d0dce3437d8e06905b93bcd7a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name DNA-Binding Proteins
131 rdf:type schema:DefinedTerm
132 N975366efc6934ba6a0413927f1044404 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
133 schema:familyName MacLeod
134 schema:givenName Ruth
135 rdf:type schema:Person
136 N97563a7408a240f7845a762b49635121 schema:issueNumber 3
137 rdf:type schema:PublicationIssue
138 Nb743d4bfd81e470aa596510217a2edff rdf:first sg:person.0752707205.75
139 rdf:rest N0614c92ea98843bdb73d3b86818ee750
140 Nbe66ef4c890641708885e7390ce56be3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Animals
142 rdf:type schema:DefinedTerm
143 Ncf64c9c9f3d24cf1838002ede8cf4d91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Mice
145 rdf:type schema:DefinedTerm
146 Nd3ea27aa38eb4ef29c10fc79b9c8ccff schema:volumeNumber 8
147 rdf:type schema:PublicationVolume
148 Ne935f718571c4cea9a6a019f9a3f80e0 rdf:first N975366efc6934ba6a0413927f1044404
149 rdf:rest N28d664bf3f7049459de75ee0c426cbb9
150 Nea6275620b7d4d83a852c6153945798f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Epigenesis, Genetic
152 rdf:type schema:DefinedTerm
153 Nee033751192b4c22aa96018c8d501615 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Pluripotent Stem Cells
155 rdf:type schema:DefinedTerm
156 Nefaa140b48f34458ad585001f25707e8 schema:name pubmed_id
157 schema:value 16462733
158 rdf:type schema:PropertyValue
159 Nf0f7609eab09420c8141d7c505327545 rdf:first sg:person.0654621540.41
160 rdf:rest Ne935f718571c4cea9a6a019f9a3f80e0
161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biological Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
165 schema:name Genetics
166 rdf:type schema:DefinedTerm
167 sg:grant.2758772 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1372
168 rdf:type schema:MonetaryGrant
169 sg:grant.2766191 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1372
170 rdf:type schema:MonetaryGrant
171 sg:grant.3632311 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1372
172 rdf:type schema:MonetaryGrant
173 sg:grant.3632816 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1372
174 rdf:type schema:MonetaryGrant
175 sg:journal.1021344 schema:issn 1465-7392
176 1476-4679
177 schema:name Nature Cell Biology
178 rdf:type schema:Periodical
179 sg:person.01350502174.86 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
180 schema:familyName Kaji
181 schema:givenName Keisuke
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350502174.86
183 rdf:type schema:Person
184 sg:person.01355477547.03 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
185 schema:familyName Hendrich
186 schema:givenName Brian
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355477547.03
188 rdf:type schema:Person
189 sg:person.0654621540.41 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
190 schema:familyName Caballero
191 schema:givenName Isabel Martín
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654621540.41
193 rdf:type schema:Person
194 sg:person.0752707205.75 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
195 schema:familyName Wilson
196 schema:givenName Valerie A.
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752707205.75
198 rdf:type schema:Person
199 sg:person.07725017617.42 schema:affiliation https://www.grid.ac/institutes/grid.4305.2
200 schema:familyName Nichols
201 schema:givenName Jennifer
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07725017617.42
203 rdf:type schema:Person
204 sg:pub.10.1007/bf01666137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026225001
205 https://doi.org/10.1007/bf01666137
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/12664 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039527067
208 https://doi.org/10.1038/12664
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/343657a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001498814
211 https://doi.org/10.1038/343657a0
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nature00927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012360268
214 https://doi.org/10.1038/nature00927
215 rdf:type schema:CreativeWork
216 sg:pub.10.1038/nature01646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042028812
217 https://doi.org/10.1038/nature01646
218 rdf:type schema:CreativeWork
219 sg:pub.10.1038/nature729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050743069
220 https://doi.org/10.1038/nature729
221 rdf:type schema:CreativeWork
222 sg:pub.10.1038/nbt729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046397624
223 https://doi.org/10.1038/nbt729
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/nbt780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011057005
226 https://doi.org/10.1038/nbt780
227 rdf:type schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1077500611 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1079089305 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1080020802 schema:CreativeWork
231 https://doi.org/10.1006/dbio.1996.0165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029442546
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/j.cell.2005.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002417372
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/s0092-8674(03)00392-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004929245
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0168-9525(03)00080-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011684230
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0896-6273(00)00083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005164056
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0925-4773(02)00002-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013502294
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0925-4773(98)00056-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005744835
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s0952-7915(99)00065-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009410516
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1073/pnas.72.4.1441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040962276
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1073/pnas.92.8.3566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040735545
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1074/jbc.m203455200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005518002
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1096/fj.14.11.1577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060111312
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1096/fj.99-0810com schema:sameAs https://app.dimensions.ai/details/publication/pub.1100513376
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1101/gad.12.13.2048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033063759
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1101/gad.13.15.1924 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012363514
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1101/gad.194101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038747920
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1126/science.282.5396.2072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062563523
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1128/mcb.18.11.6538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013470999
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1128/mcb.22.5.1526-1536.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006838536
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1128/mcb.24.20.8862-8871.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041763929
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1242/dev.00366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029438813
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1242/dev.00893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033869742
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1242/dev.01415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026113218
276 rdf:type schema:CreativeWork
277 https://doi.org/10.4049/jimmunol.167.11.6073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022803416
278 rdf:type schema:CreativeWork
279 https://www.grid.ac/institutes/grid.4305.2 schema:alternateName University of Edinburgh
280 schema:name Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JQ, Scotland, UK.
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...