Myosin II functions in actin-bundle turnover in neuronal growth cones View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-03

AUTHORS

Nelson A. Medeiros, Dylan T. Burnette, Paul Forscher

ABSTRACT

Retrograde actin flow works in concert with cell adhesion to generate traction forces that are involved in axon guidance in neuronal growth cones. Myosins have been implicated in retrograde flow, but identification of the specific myosin subtype(s) involved has been controversial. Using fluorescent speckle microscopy (FSM) to assess actin dynamics, we report that inhibition of myosin II alone decreases retrograde flow by 51% and the remaining flow can be almost fully accounted for by the 'push' of plus-end actin assembly at the leading edge of the growth cone. Interestingly, actin bundles that are associated with filopodium roots elongated by approximately 83% after inhibition of myosin II. This unexpected result was due to decreased rates of actin-bundle severing near their proximal (minus or pointed) ends which are located in the transition zone of the growth cone. Our study reveals a mechanism for the regulation of actin-bundle length by myosin II that is dependent on actin-bundle severing, and demonstrate that retrograde flow is a steady state that depends on both myosin II contractility and actin-network treadmilling. More... »

PAGES

216-226

References to SciGraph publications

  • 2005-06. Laminin stimulates and guides axonal outgrowth via growth cone myosin II activity in NATURE NEUROSCIENCE
  • 2004-08. Specificity of blebbistatin, an inhibitor of myosin II in JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
  • 2005-04. The structural basis of blebbistatin inhibition and specificity for myosin II in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ncb1367

    DOI

    http://dx.doi.org/10.1038/ncb1367

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000775238

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16501565


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Actins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Aplysia", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Axons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescent Dyes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Growth Cones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Heterocyclic Compounds, 4 or More Rings", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunohistochemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Fluorescence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Myosin Type II", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neurons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pseudopodia", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Yale University", 
              "id": "https://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Interdepartmental Neuroscience Program, Yale University, New Haven, CT 06520, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Medeiros", 
            "givenName": "Nelson A.", 
            "id": "sg:person.01270621305.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270621305.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Yale University", 
              "id": "https://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Dept. of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Burnette", 
            "givenName": "Dylan T.", 
            "id": "sg:person.01106257505.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106257505.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Yale University", 
              "id": "https://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Interdepartmental Neuroscience Program, Yale University, New Haven, CT 06520, USA.", 
                "Dept. of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Forscher", 
            "givenName": "Paul", 
            "id": "sg:person.01040144305.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040144305.45"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1523/jneurosci.2836-04.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000089193"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.107.4.1505", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004085960"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200202028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005216095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.131.4.989", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006903677"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.146.5.1097", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009704184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.146.5.1097", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009704184"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/neu.10282", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010793765"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.139.2.397", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013330863"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m205099200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013591119"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.00335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019689096"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200412043", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020419401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200412043", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020419401"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2005.02.055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022479543"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.272.19.12257", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022491866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2003.08.050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022598721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2003.08.050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022598721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1466", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024552967", 
              "https://doi.org/10.1038/nn1466"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1466", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024552967", 
              "https://doi.org/10.1038/nn1466"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1466", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024552967", 
              "https://doi.org/10.1038/nn1466"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0896-6273(03)00754-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027488482"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1081412", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028126150"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cub.2005.03.042", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028513718"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200210174", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028953059"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1471-4159.2003.02058.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029220955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0896-6273(88)90124-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029394923"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m405319200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032462583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb908", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032477432", 
              "https://doi.org/10.1038/nsmb908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb908", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032477432", 
              "https://doi.org/10.1038/nsmb908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/cm.20051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033144218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0896-6273(95)90220-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033685315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200203038", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034779726"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.neuro.26.010302.081139", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036244940"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/cm.10149", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039339328"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10974-004-6060-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040698626", 
              "https://doi.org/10.1007/s10974-004-6060-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.83.17.6272", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043687780"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0896-6273(04)00108-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044366917"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.119.5.1219", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044456240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0896-6273(00)80097-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045117503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/002215540305100403", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045406846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/002215540305100403", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045406846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e03-03-0187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045599006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbrc.2004.06.045", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046800060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.200204140", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048239686"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2795(75)90222-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048939516"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2795(75)90222-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048939516"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0960-9822(07)00515-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053746065"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00252a003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055167139"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0490284", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055199507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0490284", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055199507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.3055292", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062586167"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.3277283", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062603648"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1523/jneurosci.19-18-07971.1999", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074518293"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1523/jneurosci.21-16-06159.2001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074859323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1523/jneurosci.07-11-03600.1987", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079412069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082925223", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083039139", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2006-03", 
        "datePublishedReg": "2006-03-01", 
        "description": "Retrograde actin flow works in concert with cell adhesion to generate traction forces that are involved in axon guidance in neuronal growth cones. Myosins have been implicated in retrograde flow, but identification of the specific myosin subtype(s) involved has been controversial. Using fluorescent speckle microscopy (FSM) to assess actin dynamics, we report that inhibition of myosin II alone decreases retrograde flow by 51% and the remaining flow can be almost fully accounted for by the 'push' of plus-end actin assembly at the leading edge of the growth cone. Interestingly, actin bundles that are associated with filopodium roots elongated by approximately 83% after inhibition of myosin II. This unexpected result was due to decreased rates of actin-bundle severing near their proximal (minus or pointed) ends which are located in the transition zone of the growth cone. Our study reveals a mechanism for the regulation of actin-bundle length by myosin II that is dependent on actin-bundle severing, and demonstrate that retrograde flow is a steady state that depends on both myosin II contractility and actin-network treadmilling.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/ncb1367", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2561411", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2557041", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1021344", 
            "issn": [
              "1465-7392", 
              "1476-4679"
            ], 
            "name": "Nature Cell Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "8"
          }
        ], 
        "name": "Myosin II functions in actin-bundle turnover in neuronal growth cones", 
        "pagination": "216-226", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "03b10a99c9838b73b59970ab9f3eb9d5ccb52859d7c9b49476509d71bcbf7f59"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16501565"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "100890575"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ncb1367"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1000775238"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ncb1367", 
          "https://app.dimensions.ai/details/publication/pub.1000775238"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:21", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87079_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/ncb1367"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ncb1367'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ncb1367'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ncb1367'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ncb1367'


     

    This table displays all metadata directly associated to this object as RDF triples.

    282 TRIPLES      21 PREDICATES      89 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ncb1367 schema:about N0fcefc4e639c422e96c4dd53e314e3b0
    2 N2e42fd09309947a1a94398236c5429cd
    3 N3f853d380bf54b27b05037946ad75f0f
    4 N41eb4c326f1b4b3ebdd6003bbde1f294
    5 N5139005eecc04e43ac9c31ab4424ba5c
    6 N5408bc2d273643d6987b80f643af9222
    7 N69da2ddb63c7497aa984a8ffca36aa1a
    8 N6af16349eca84bbd948f9fc5f087e14a
    9 N71a53e293bf946cca114e443ea5ee3d5
    10 N739f1bd22ab04bf79929ba7e96b1fd9b
    11 N8471f79acf6a4fcb98b9b154bca21a91
    12 Ne4a3f28b79304144bcca551cf046ef25
    13 Nf278f678058540ba929316e0c74ec4e8
    14 anzsrc-for:06
    15 anzsrc-for:0601
    16 schema:author Nd18e8792557f4701a80acf2d8f91ff1b
    17 schema:citation sg:pub.10.1007/s10974-004-6060-7
    18 sg:pub.10.1038/nn1466
    19 sg:pub.10.1038/nsmb908
    20 https://app.dimensions.ai/details/publication/pub.1082925223
    21 https://app.dimensions.ai/details/publication/pub.1083039139
    22 https://doi.org/10.1002/cm.10149
    23 https://doi.org/10.1002/cm.20051
    24 https://doi.org/10.1002/neu.10282
    25 https://doi.org/10.1016/0005-2795(75)90222-6
    26 https://doi.org/10.1016/0896-6273(88)90124-9
    27 https://doi.org/10.1016/0896-6273(95)90220-1
    28 https://doi.org/10.1016/j.bbrc.2004.06.045
    29 https://doi.org/10.1016/j.cub.2003.08.050
    30 https://doi.org/10.1016/j.cub.2005.02.055
    31 https://doi.org/10.1016/j.cub.2005.03.042
    32 https://doi.org/10.1016/s0896-6273(00)80097-5
    33 https://doi.org/10.1016/s0896-6273(03)00754-2
    34 https://doi.org/10.1016/s0896-6273(04)00108-4
    35 https://doi.org/10.1016/s0960-9822(07)00515-5
    36 https://doi.org/10.1021/bi00252a003
    37 https://doi.org/10.1021/bi0490284
    38 https://doi.org/10.1046/j.1471-4159.2003.02058.x
    39 https://doi.org/10.1073/pnas.83.17.6272
    40 https://doi.org/10.1074/jbc.272.19.12257
    41 https://doi.org/10.1074/jbc.m205099200
    42 https://doi.org/10.1074/jbc.m405319200
    43 https://doi.org/10.1083/jcb.107.4.1505
    44 https://doi.org/10.1083/jcb.119.5.1219
    45 https://doi.org/10.1083/jcb.131.4.989
    46 https://doi.org/10.1083/jcb.139.2.397
    47 https://doi.org/10.1083/jcb.146.5.1097
    48 https://doi.org/10.1083/jcb.200202028
    49 https://doi.org/10.1083/jcb.200203038
    50 https://doi.org/10.1083/jcb.200204140
    51 https://doi.org/10.1083/jcb.200210174
    52 https://doi.org/10.1083/jcb.200412043
    53 https://doi.org/10.1091/mbc.e03-03-0187
    54 https://doi.org/10.1126/science.1081412
    55 https://doi.org/10.1126/science.3055292
    56 https://doi.org/10.1126/science.3277283
    57 https://doi.org/10.1146/annurev.neuro.26.010302.081139
    58 https://doi.org/10.1177/002215540305100403
    59 https://doi.org/10.1242/jcs.00335
    60 https://doi.org/10.1523/jneurosci.07-11-03600.1987
    61 https://doi.org/10.1523/jneurosci.19-18-07971.1999
    62 https://doi.org/10.1523/jneurosci.21-16-06159.2001
    63 https://doi.org/10.1523/jneurosci.2836-04.2004
    64 schema:datePublished 2006-03
    65 schema:datePublishedReg 2006-03-01
    66 schema:description Retrograde actin flow works in concert with cell adhesion to generate traction forces that are involved in axon guidance in neuronal growth cones. Myosins have been implicated in retrograde flow, but identification of the specific myosin subtype(s) involved has been controversial. Using fluorescent speckle microscopy (FSM) to assess actin dynamics, we report that inhibition of myosin II alone decreases retrograde flow by 51% and the remaining flow can be almost fully accounted for by the 'push' of plus-end actin assembly at the leading edge of the growth cone. Interestingly, actin bundles that are associated with filopodium roots elongated by approximately 83% after inhibition of myosin II. This unexpected result was due to decreased rates of actin-bundle severing near their proximal (minus or pointed) ends which are located in the transition zone of the growth cone. Our study reveals a mechanism for the regulation of actin-bundle length by myosin II that is dependent on actin-bundle severing, and demonstrate that retrograde flow is a steady state that depends on both myosin II contractility and actin-network treadmilling.
    67 schema:genre research_article
    68 schema:inLanguage en
    69 schema:isAccessibleForFree false
    70 schema:isPartOf N52bca4f261804b5fb5f9446c744961ab
    71 N694c8b7850544af195c840da29308cc1
    72 sg:journal.1021344
    73 schema:name Myosin II functions in actin-bundle turnover in neuronal growth cones
    74 schema:pagination 216-226
    75 schema:productId N0ccc67b0e6dc4f4c9bb5d68f6f6a6f01
    76 N16a93ea49fde45c1a7251c321ed2b0a4
    77 N241f2a731b3a4e9fbd0305770f8818c0
    78 N2599ae73d81645eaac722c450f55d61d
    79 N6c5b568e322143f0a968e0a42dc44c91
    80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000775238
    81 https://doi.org/10.1038/ncb1367
    82 schema:sdDatePublished 2019-04-11T12:21
    83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    84 schema:sdPublisher Nb67528a93b364e77ac60eacb9895fba1
    85 schema:url http://www.nature.com/articles/ncb1367
    86 sgo:license sg:explorer/license/
    87 sgo:sdDataset articles
    88 rdf:type schema:ScholarlyArticle
    89 N0ccc67b0e6dc4f4c9bb5d68f6f6a6f01 schema:name pubmed_id
    90 schema:value 16501565
    91 rdf:type schema:PropertyValue
    92 N0fcefc4e639c422e96c4dd53e314e3b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Immunohistochemistry
    94 rdf:type schema:DefinedTerm
    95 N16a93ea49fde45c1a7251c321ed2b0a4 schema:name nlm_unique_id
    96 schema:value 100890575
    97 rdf:type schema:PropertyValue
    98 N241f2a731b3a4e9fbd0305770f8818c0 schema:name doi
    99 schema:value 10.1038/ncb1367
    100 rdf:type schema:PropertyValue
    101 N2599ae73d81645eaac722c450f55d61d schema:name dimensions_id
    102 schema:value pub.1000775238
    103 rdf:type schema:PropertyValue
    104 N2e42fd09309947a1a94398236c5429cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Axons
    106 rdf:type schema:DefinedTerm
    107 N3f853d380bf54b27b05037946ad75f0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Myosin Type II
    109 rdf:type schema:DefinedTerm
    110 N41eb4c326f1b4b3ebdd6003bbde1f294 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Neurons
    112 rdf:type schema:DefinedTerm
    113 N50cd6f3714ef4010a975b996fc39f1d4 rdf:first sg:person.01106257505.85
    114 rdf:rest Nd768854b7c26442eb8511fb0885f8fe8
    115 N5139005eecc04e43ac9c31ab4424ba5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Heterocyclic Compounds, 4 or More Rings
    117 rdf:type schema:DefinedTerm
    118 N52bca4f261804b5fb5f9446c744961ab schema:volumeNumber 8
    119 rdf:type schema:PublicationVolume
    120 N5408bc2d273643d6987b80f643af9222 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Aplysia
    122 rdf:type schema:DefinedTerm
    123 N694c8b7850544af195c840da29308cc1 schema:issueNumber 3
    124 rdf:type schema:PublicationIssue
    125 N69da2ddb63c7497aa984a8ffca36aa1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Cells, Cultured
    127 rdf:type schema:DefinedTerm
    128 N6af16349eca84bbd948f9fc5f087e14a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Pseudopodia
    130 rdf:type schema:DefinedTerm
    131 N6c5b568e322143f0a968e0a42dc44c91 schema:name readcube_id
    132 schema:value 03b10a99c9838b73b59970ab9f3eb9d5ccb52859d7c9b49476509d71bcbf7f59
    133 rdf:type schema:PropertyValue
    134 N71a53e293bf946cca114e443ea5ee3d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Growth Cones
    136 rdf:type schema:DefinedTerm
    137 N739f1bd22ab04bf79929ba7e96b1fd9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Animals
    139 rdf:type schema:DefinedTerm
    140 N8471f79acf6a4fcb98b9b154bca21a91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Actins
    142 rdf:type schema:DefinedTerm
    143 Nb67528a93b364e77ac60eacb9895fba1 schema:name Springer Nature - SN SciGraph project
    144 rdf:type schema:Organization
    145 Nd18e8792557f4701a80acf2d8f91ff1b rdf:first sg:person.01270621305.52
    146 rdf:rest N50cd6f3714ef4010a975b996fc39f1d4
    147 Nd768854b7c26442eb8511fb0885f8fe8 rdf:first sg:person.01040144305.45
    148 rdf:rest rdf:nil
    149 Ne4a3f28b79304144bcca551cf046ef25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Microscopy, Fluorescence
    151 rdf:type schema:DefinedTerm
    152 Nf278f678058540ba929316e0c74ec4e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Fluorescent Dyes
    154 rdf:type schema:DefinedTerm
    155 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    156 schema:name Biological Sciences
    157 rdf:type schema:DefinedTerm
    158 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    159 schema:name Biochemistry and Cell Biology
    160 rdf:type schema:DefinedTerm
    161 sg:grant.2557041 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1367
    162 rdf:type schema:MonetaryGrant
    163 sg:grant.2561411 http://pending.schema.org/fundedItem sg:pub.10.1038/ncb1367
    164 rdf:type schema:MonetaryGrant
    165 sg:journal.1021344 schema:issn 1465-7392
    166 1476-4679
    167 schema:name Nature Cell Biology
    168 rdf:type schema:Periodical
    169 sg:person.01040144305.45 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
    170 schema:familyName Forscher
    171 schema:givenName Paul
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040144305.45
    173 rdf:type schema:Person
    174 sg:person.01106257505.85 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
    175 schema:familyName Burnette
    176 schema:givenName Dylan T.
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106257505.85
    178 rdf:type schema:Person
    179 sg:person.01270621305.52 schema:affiliation https://www.grid.ac/institutes/grid.47100.32
    180 schema:familyName Medeiros
    181 schema:givenName Nelson A.
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270621305.52
    183 rdf:type schema:Person
    184 sg:pub.10.1007/s10974-004-6060-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040698626
    185 https://doi.org/10.1007/s10974-004-6060-7
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/nn1466 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024552967
    188 https://doi.org/10.1038/nn1466
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/nsmb908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032477432
    191 https://doi.org/10.1038/nsmb908
    192 rdf:type schema:CreativeWork
    193 https://app.dimensions.ai/details/publication/pub.1082925223 schema:CreativeWork
    194 https://app.dimensions.ai/details/publication/pub.1083039139 schema:CreativeWork
    195 https://doi.org/10.1002/cm.10149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039339328
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1002/cm.20051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033144218
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1002/neu.10282 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010793765
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/0005-2795(75)90222-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048939516
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1016/0896-6273(88)90124-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029394923
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1016/0896-6273(95)90220-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033685315
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1016/j.bbrc.2004.06.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046800060
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1016/j.cub.2003.08.050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022598721
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1016/j.cub.2005.02.055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022479543
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1016/j.cub.2005.03.042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028513718
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1016/s0896-6273(00)80097-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045117503
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1016/s0896-6273(03)00754-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027488482
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1016/s0896-6273(04)00108-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044366917
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1016/s0960-9822(07)00515-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053746065
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1021/bi00252a003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055167139
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1021/bi0490284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055199507
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1046/j.1471-4159.2003.02058.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029220955
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1073/pnas.83.17.6272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043687780
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1074/jbc.272.19.12257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022491866
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1074/jbc.m205099200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013591119
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1074/jbc.m405319200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032462583
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1083/jcb.107.4.1505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004085960
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1083/jcb.119.5.1219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044456240
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1083/jcb.131.4.989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006903677
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1083/jcb.139.2.397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013330863
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1083/jcb.146.5.1097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009704184
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1083/jcb.200202028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005216095
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1083/jcb.200203038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034779726
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1083/jcb.200204140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048239686
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1083/jcb.200210174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028953059
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1083/jcb.200412043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020419401
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1091/mbc.e03-03-0187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045599006
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1126/science.1081412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028126150
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1126/science.3055292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062586167
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1126/science.3277283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062603648
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1146/annurev.neuro.26.010302.081139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036244940
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1177/002215540305100403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045406846
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1242/jcs.00335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019689096
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1523/jneurosci.07-11-03600.1987 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079412069
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1523/jneurosci.19-18-07971.1999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074518293
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1523/jneurosci.21-16-06159.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074859323
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1523/jneurosci.2836-04.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000089193
    278 rdf:type schema:CreativeWork
    279 https://www.grid.ac/institutes/grid.47100.32 schema:alternateName Yale University
    280 schema:name Dept. of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
    281 Interdepartmental Neuroscience Program, Yale University, New Haven, CT 06520, USA.
    282 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...