MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-07

AUTHORS

Jidong Liu, Marco Antonio Valencia-Sanchez, Gregory J Hannon, Roy Parker

ABSTRACT

Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs) can silence target genes through several different effector mechanisms. Whereas siRNA-directed mRNA cleavage is increasingly understood, the mechanisms by which miRNAs repress protein synthesis are obscure. Recent studies have revealed the existence of specific cytoplasmic foci, referred to herein as processing bodies (P-bodies), which contain untranslated mRNAs and can serve as sites of mRNA degradation. Here we demonstrate that Argonaute proteins--the signature components of the RNA interference (RNAi) effector complex, RISC--localize to mammalian P-bodies. Moreover, reporter mRNAs that are targeted for translational repression by endogenous or exogenous miRNAs become concentrated in P-bodies in a miRNA-dependent manner. These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis. More... »

PAGES

719-723

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ncb1274

DOI

http://dx.doi.org/10.1038/ncb1274

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014076938

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15937477


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