Expression profiling reveals off-target gene regulation by RNAi View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-06

AUTHORS

Aimee L Jackson, Steven R Bartz, Janell Schelter, Sumire V Kobayashi, Julja Burchard, Mao Mao, Bin Li, Guy Cavet, Peter S Linsley

ABSTRACT

RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity. More... »

PAGES

635-637

Journal

TITLE

Nature Biotechnology

ISSUE

6

VOLUME

21

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt831

    DOI

    http://dx.doi.org/10.1038/nbt831

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021890587

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12754523


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