A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-04

AUTHORS

Nick de Vetten, Anne-Marie Wolters, Krit Raemakers, Ingrid van der Meer, Renaldo ter Stege, Els Heeres, Paul Heeres, Richard Visser

ABSTRACT

It is generally thought that transformation of plant cells using Agrobacterium tumefaciens occurs at a very low frequency. Therefore, selection marker genes are used to identify the rare plants that have taken up foreign DNA. Genes encoding antibiotic and herbicide resistance are widely used for this purpose in plant transformation. Over the past several years, consumer and environmental groups have expressed concern about the use of antibiotic- and herbicide-resistance genes from an ecological and food safety perspective. Although no scientific basis has been determined for these concerns, generating marker-free plants would certainly contribute to the public acceptance of transgenic crops. Several methods have been reported to create marker gene-free transformed plants, for example co-transformation, transposable elements, site-specific recombination, or intrachromosomal recombination. Not only are most of these systems time-consuming and inefficient, but they are also employed on the assumption that isolation of transformants without a selective marker gene is not feasible. Here we present a method that permits the identification of transgenic plants without the use of selectable markers. This strategy relies on the transformation of tissue explants or cells with a virulent A. tumefaciens strain and selection of transformed cells or shoots after PCR analysis. Incubation of potato explants with A. tumefaciens strain AGL0 resulted in transformed shoots at an efficiency of 1-5% of the harvested shoots, depending on the potato genotype used. Because this system does not require genetic segregation or site-specific DNA-deletion systems to remove marker genes, it may provide a reliable and efficient tool for generating transgenic plants for commercial use, especially in vegetatively propagated species like potato and cassava. More... »

PAGES

439-442

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

4

VOLUME

21

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt801

DOI

http://dx.doi.org/10.1038/nbt801

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042004278

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12627169


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Agriculture", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Agrobacterium tumefaciens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Dominant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Markers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Variation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Solanum tuberosum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Species Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "AVEBE, AVEBE-weg 1, 9607 PT Foxhol, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Vetten", 
        "givenName": "Nick", 
        "id": "sg:person.01367640060.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367640060.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wageningen University & Research", 
          "id": "https://www.grid.ac/institutes/grid.4818.5", 
          "name": [
            "Laboratory of Plant Breeding, Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wolters", 
        "givenName": "Anne-Marie", 
        "id": "sg:person.01307134176.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307134176.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wageningen University & Research", 
          "id": "https://www.grid.ac/institutes/grid.4818.5", 
          "name": [
            "Laboratory of Plant Breeding, Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raemakers", 
        "givenName": "Krit", 
        "id": "sg:person.0644537220.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644537220.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wageningen University & Research", 
          "id": "https://www.grid.ac/institutes/grid.4818.5", 
          "name": [
            "Plant Research International, P.O. Box 16, 6700 AJ Wageningen, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "van der Meer", 
        "givenName": "Ingrid", 
        "id": "sg:person.01140336306.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140336306.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "AVEBE, AVEBE-weg 1, 9607 PT Foxhol, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stege", 
        "givenName": "Renaldo ter", 
        "id": "sg:person.07762374763.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07762374763.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Breeding Institute KARNA, Valtherblokken Zuid 40, 7876 TC Valthermond, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heeres", 
        "givenName": "Els", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Breeding Institute KARNA, Valtherblokken Zuid 40, 7876 TC Valthermond, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heeres", 
        "givenName": "Paul", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wageningen University & Research", 
          "id": "https://www.grid.ac/institutes/grid.4818.5", 
          "name": [
            "Laboratory of Plant Breeding, Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Visser", 
        "givenName": "Richard", 
        "id": "sg:person.07374343732.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07374343732.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1023/a:1017530932536", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002509077", 
          "https://doi.org/10.1023/a:1017530932536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00437912", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002806059", 
          "https://doi.org/10.1007/bf00437912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.6.8.1032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003033873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/84362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009864125", 
          "https://doi.org/10.1038/84362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/84362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009864125", 
          "https://doi.org/10.1038/84362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74515", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011877455", 
          "https://doi.org/10.1038/74515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/74515", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011877455", 
          "https://doi.org/10.1038/74515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00290722", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012152521", 
          "https://doi.org/10.1007/bf00290722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1091-963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012182392", 
          "https://doi.org/10.1038/nbt1091-963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1015689112703", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012973939", 
          "https://doi.org/10.1023/a:1015689112703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/303179a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018421874", 
          "https://doi.org/10.1038/303179a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1997.11050945.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018823521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/84428", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022691725", 
          "https://doi.org/10.1038/84428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/84428", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022691725", 
          "https://doi.org/10.1038/84428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.23.10558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026183757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006184221051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029358994", 
          "https://doi.org/10.1023/a:1006184221051"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/304184a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030766221", 
          "https://doi.org/10.1038/304184a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.6.2117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032218295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1996.10010165.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038680141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1998.00079.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044385271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1998.00079.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044385271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.233.4762.478", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062532803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.168.3.1291-1301.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062715363"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-04", 
    "datePublishedReg": "2003-04-01", 
    "description": "It is generally thought that transformation of plant cells using Agrobacterium tumefaciens occurs at a very low frequency. Therefore, selection marker genes are used to identify the rare plants that have taken up foreign DNA. Genes encoding antibiotic and herbicide resistance are widely used for this purpose in plant transformation. Over the past several years, consumer and environmental groups have expressed concern about the use of antibiotic- and herbicide-resistance genes from an ecological and food safety perspective. Although no scientific basis has been determined for these concerns, generating marker-free plants would certainly contribute to the public acceptance of transgenic crops. Several methods have been reported to create marker gene-free transformed plants, for example co-transformation, transposable elements, site-specific recombination, or intrachromosomal recombination. Not only are most of these systems time-consuming and inefficient, but they are also employed on the assumption that isolation of transformants without a selective marker gene is not feasible. Here we present a method that permits the identification of transgenic plants without the use of selectable markers. This strategy relies on the transformation of tissue explants or cells with a virulent A. tumefaciens strain and selection of transformed cells or shoots after PCR analysis. Incubation of potato explants with A. tumefaciens strain AGL0 resulted in transformed shoots at an efficiency of 1-5% of the harvested shoots, depending on the potato genotype used. Because this system does not require genetic segregation or site-specific DNA-deletion systems to remove marker genes, it may provide a reliable and efficient tool for generating transgenic plants for commercial use, especially in vegetatively propagated species like potato and cassava.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt801", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1115214", 
        "issn": [
          "1087-0156", 
          "1546-1696"
        ], 
        "name": "Nature Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "21"
      }
    ], 
    "name": "A transformation method for obtaining marker-free plants of a\n\t\tcross-pollinating and vegetatively propagated crop", 
    "pagination": "439-442", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c8169e74179bf9af970f9e652ec91dccf2d3ecd6778145f21640e1cc3cdd6366"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12627169"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9604648"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt801"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1042004278"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt801", 
      "https://app.dimensions.ai/details/publication/pub.1042004278"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87115_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/nbt801"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt801'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt801'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt801'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt801'


 

This table displays all metadata directly associated to this object as RDF triples.

248 TRIPLES      21 PREDICATES      62 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt801 schema:about N119a0a262c674c2daf4e049b6183fb85
2 N13ba527f4dae45849b60ea134b146517
3 N14c63217ae5b4ca2a1c72b404e50ecdd
4 N1655784cffb8436e9c0e0b9ea8cd073e
5 N3c71be92786b4f29869a1a0d5345dbba
6 N40b4c9c86ace4e9cac9f00dfbd428b17
7 N7d0c63e7a95345128b5f27ba254a6b2f
8 N88f5fba45e404b008109093a8fd50ce8
9 Nbf653ca80c2841448d0a590dcff9868b
10 Ncee5f4b54ef44ea2a12d53c40101d4a5
11 Nd3f184283742420dbdd2a2e84e2fba76
12 Ndc60ca029bd643cf8636720c00eab66a
13 Ne6ae83bf095d4ce889c7fcbe35613d2f
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N61383d9b186f493bb769073979986d6c
17 schema:citation sg:pub.10.1007/bf00290722
18 sg:pub.10.1007/bf00437912
19 sg:pub.10.1023/a:1006184221051
20 sg:pub.10.1023/a:1015689112703
21 sg:pub.10.1023/a:1017530932536
22 sg:pub.10.1038/303179a0
23 sg:pub.10.1038/304184a0
24 sg:pub.10.1038/74515
25 sg:pub.10.1038/84362
26 sg:pub.10.1038/84428
27 sg:pub.10.1038/nbt1091-963
28 https://doi.org/10.1046/j.1365-313x.1996.10010165.x
29 https://doi.org/10.1046/j.1365-313x.1997.11050945.x
30 https://doi.org/10.1046/j.1365-313x.1998.00079.x
31 https://doi.org/10.1073/pnas.88.23.10558
32 https://doi.org/10.1073/pnas.94.6.2117
33 https://doi.org/10.1105/tpc.6.8.1032
34 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
35 https://doi.org/10.1126/science.233.4762.478
36 https://doi.org/10.1128/jb.168.3.1291-1301.1986
37 schema:datePublished 2003-04
38 schema:datePublishedReg 2003-04-01
39 schema:description It is generally thought that transformation of plant cells using Agrobacterium tumefaciens occurs at a very low frequency. Therefore, selection marker genes are used to identify the rare plants that have taken up foreign DNA. Genes encoding antibiotic and herbicide resistance are widely used for this purpose in plant transformation. Over the past several years, consumer and environmental groups have expressed concern about the use of antibiotic- and herbicide-resistance genes from an ecological and food safety perspective. Although no scientific basis has been determined for these concerns, generating marker-free plants would certainly contribute to the public acceptance of transgenic crops. Several methods have been reported to create marker gene-free transformed plants, for example co-transformation, transposable elements, site-specific recombination, or intrachromosomal recombination. Not only are most of these systems time-consuming and inefficient, but they are also employed on the assumption that isolation of transformants without a selective marker gene is not feasible. Here we present a method that permits the identification of transgenic plants without the use of selectable markers. This strategy relies on the transformation of tissue explants or cells with a virulent A. tumefaciens strain and selection of transformed cells or shoots after PCR analysis. Incubation of potato explants with A. tumefaciens strain AGL0 resulted in transformed shoots at an efficiency of 1-5% of the harvested shoots, depending on the potato genotype used. Because this system does not require genetic segregation or site-specific DNA-deletion systems to remove marker genes, it may provide a reliable and efficient tool for generating transgenic plants for commercial use, especially in vegetatively propagated species like potato and cassava.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree false
43 schema:isPartOf N711ac2efb60c4bec9a98fec6e678abcb
44 Nbc08918455aa490393b1ae22bf7cf9db
45 sg:journal.1115214
46 schema:name A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop
47 schema:pagination 439-442
48 schema:productId N4af0aabf30a341859559df608587f183
49 N5b8308b601964ba3973328afa5914bf6
50 N652c0ec5da46405f98353d67985637ff
51 N65806a6a4a3e45e192677aa6f3062ccf
52 Nb4aee4b8e7f444caa1408dd330ee840c
53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042004278
54 https://doi.org/10.1038/nbt801
55 schema:sdDatePublished 2019-04-11T12:27
56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
57 schema:sdPublisher Nce68f7d25381483e9f5b839de162a675
58 schema:url http://www.nature.com/articles/nbt801
59 sgo:license sg:explorer/license/
60 sgo:sdDataset articles
61 rdf:type schema:ScholarlyArticle
62 N052ebdf576174de59137c5a8a3654c6f schema:name Breeding Institute KARNA, Valtherblokken Zuid 40, 7876 TC Valthermond, The Netherlands.
63 rdf:type schema:Organization
64 N0ce616453fe74dd9b37ae4e420e0dd3f rdf:first sg:person.0644537220.64
65 rdf:rest Nac3099bbe56841bb8094144cf6a6cb52
66 N119a0a262c674c2daf4e049b6183fb85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Gene Expression Regulation, Plant
68 rdf:type schema:DefinedTerm
69 N13ba527f4dae45849b60ea134b146517 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Plants, Genetically Modified
71 rdf:type schema:DefinedTerm
72 N14c63217ae5b4ca2a1c72b404e50ecdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Transformation, Genetic
74 rdf:type schema:DefinedTerm
75 N1655784cffb8436e9c0e0b9ea8cd073e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Genetic Variation
77 rdf:type schema:DefinedTerm
78 N1da8a8d45f78425bb8feba0d27e7fa24 rdf:first sg:person.01307134176.66
79 rdf:rest N0ce616453fe74dd9b37ae4e420e0dd3f
80 N352d0642e3374ad2b0dc8f836b4268d6 rdf:first N95a11a6173ce45fb874537467914d41f
81 rdf:rest N5e22a6e1a5834d8696e05481e960eccd
82 N35b8bec86f224c5c88fa655b0f572db2 schema:name AVEBE, AVEBE-weg 1, 9607 PT Foxhol, The Netherlands.
83 rdf:type schema:Organization
84 N3c71be92786b4f29869a1a0d5345dbba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Gene Transfer Techniques
86 rdf:type schema:DefinedTerm
87 N3c761281f85d4431b7f396bef9c20947 schema:affiliation N052ebdf576174de59137c5a8a3654c6f
88 schema:familyName Heeres
89 schema:givenName Paul
90 rdf:type schema:Person
91 N40b4c9c86ace4e9cac9f00dfbd428b17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Genes, Dominant
93 rdf:type schema:DefinedTerm
94 N4719cf8be56b48a9ba9360a68e8c4a41 rdf:first sg:person.07762374763.31
95 rdf:rest N352d0642e3374ad2b0dc8f836b4268d6
96 N483bfe55b75d4b73971e808f1d3b61ce rdf:first sg:person.07374343732.28
97 rdf:rest rdf:nil
98 N4af0aabf30a341859559df608587f183 schema:name nlm_unique_id
99 schema:value 9604648
100 rdf:type schema:PropertyValue
101 N5b8308b601964ba3973328afa5914bf6 schema:name pubmed_id
102 schema:value 12627169
103 rdf:type schema:PropertyValue
104 N5e22a6e1a5834d8696e05481e960eccd rdf:first N3c761281f85d4431b7f396bef9c20947
105 rdf:rest N483bfe55b75d4b73971e808f1d3b61ce
106 N5f0122a5287d48079c0febf33154a3e2 schema:name Breeding Institute KARNA, Valtherblokken Zuid 40, 7876 TC Valthermond, The Netherlands.
107 rdf:type schema:Organization
108 N61383d9b186f493bb769073979986d6c rdf:first sg:person.01367640060.55
109 rdf:rest N1da8a8d45f78425bb8feba0d27e7fa24
110 N652c0ec5da46405f98353d67985637ff schema:name dimensions_id
111 schema:value pub.1042004278
112 rdf:type schema:PropertyValue
113 N65806a6a4a3e45e192677aa6f3062ccf schema:name doi
114 schema:value 10.1038/nbt801
115 rdf:type schema:PropertyValue
116 N711ac2efb60c4bec9a98fec6e678abcb schema:issueNumber 4
117 rdf:type schema:PublicationIssue
118 N7d0c63e7a95345128b5f27ba254a6b2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Agriculture
120 rdf:type schema:DefinedTerm
121 N88f5fba45e404b008109093a8fd50ce8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Species Specificity
123 rdf:type schema:DefinedTerm
124 N95a11a6173ce45fb874537467914d41f schema:affiliation N5f0122a5287d48079c0febf33154a3e2
125 schema:familyName Heeres
126 schema:givenName Els
127 rdf:type schema:Person
128 Nac3099bbe56841bb8094144cf6a6cb52 rdf:first sg:person.01140336306.13
129 rdf:rest N4719cf8be56b48a9ba9360a68e8c4a41
130 Nb4aee4b8e7f444caa1408dd330ee840c schema:name readcube_id
131 schema:value c8169e74179bf9af970f9e652ec91dccf2d3ecd6778145f21640e1cc3cdd6366
132 rdf:type schema:PropertyValue
133 Nbc08918455aa490393b1ae22bf7cf9db schema:volumeNumber 21
134 rdf:type schema:PublicationVolume
135 Nbf653ca80c2841448d0a590dcff9868b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Phenotype
137 rdf:type schema:DefinedTerm
138 Nce68f7d25381483e9f5b839de162a675 schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 Ncee5f4b54ef44ea2a12d53c40101d4a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Genetic Markers
142 rdf:type schema:DefinedTerm
143 Nd3f184283742420dbdd2a2e84e2fba76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Polymerase Chain Reaction
145 rdf:type schema:DefinedTerm
146 Ndc60ca029bd643cf8636720c00eab66a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Agrobacterium tumefaciens
148 rdf:type schema:DefinedTerm
149 Ne6ae83bf095d4ce889c7fcbe35613d2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Solanum tuberosum
151 rdf:type schema:DefinedTerm
152 Nfa652a0054d64222a674615ad25b32b2 schema:name AVEBE, AVEBE-weg 1, 9607 PT Foxhol, The Netherlands.
153 rdf:type schema:Organization
154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
155 schema:name Biological Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
158 schema:name Genetics
159 rdf:type schema:DefinedTerm
160 sg:journal.1115214 schema:issn 1087-0156
161 1546-1696
162 schema:name Nature Biotechnology
163 rdf:type schema:Periodical
164 sg:person.01140336306.13 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
165 schema:familyName van der Meer
166 schema:givenName Ingrid
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140336306.13
168 rdf:type schema:Person
169 sg:person.01307134176.66 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
170 schema:familyName Wolters
171 schema:givenName Anne-Marie
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307134176.66
173 rdf:type schema:Person
174 sg:person.01367640060.55 schema:affiliation Nfa652a0054d64222a674615ad25b32b2
175 schema:familyName de Vetten
176 schema:givenName Nick
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367640060.55
178 rdf:type schema:Person
179 sg:person.0644537220.64 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
180 schema:familyName Raemakers
181 schema:givenName Krit
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644537220.64
183 rdf:type schema:Person
184 sg:person.07374343732.28 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
185 schema:familyName Visser
186 schema:givenName Richard
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07374343732.28
188 rdf:type schema:Person
189 sg:person.07762374763.31 schema:affiliation N35b8bec86f224c5c88fa655b0f572db2
190 schema:familyName Stege
191 schema:givenName Renaldo ter
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07762374763.31
193 rdf:type schema:Person
194 sg:pub.10.1007/bf00290722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012152521
195 https://doi.org/10.1007/bf00290722
196 rdf:type schema:CreativeWork
197 sg:pub.10.1007/bf00437912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002806059
198 https://doi.org/10.1007/bf00437912
199 rdf:type schema:CreativeWork
200 sg:pub.10.1023/a:1006184221051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029358994
201 https://doi.org/10.1023/a:1006184221051
202 rdf:type schema:CreativeWork
203 sg:pub.10.1023/a:1015689112703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012973939
204 https://doi.org/10.1023/a:1015689112703
205 rdf:type schema:CreativeWork
206 sg:pub.10.1023/a:1017530932536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002509077
207 https://doi.org/10.1023/a:1017530932536
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/303179a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
210 https://doi.org/10.1038/303179a0
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/304184a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030766221
213 https://doi.org/10.1038/304184a0
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/74515 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011877455
216 https://doi.org/10.1038/74515
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/84362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009864125
219 https://doi.org/10.1038/84362
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/84428 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022691725
222 https://doi.org/10.1038/84428
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nbt1091-963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012182392
225 https://doi.org/10.1038/nbt1091-963
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1046/j.1365-313x.1996.10010165.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038680141
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1046/j.1365-313x.1997.11050945.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018823521
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1046/j.1365-313x.1998.00079.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044385271
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1073/pnas.88.23.10558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026183757
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.94.6.2117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032218295
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1105/tpc.6.8.1032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003033873
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1126/science.233.4762.478 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062532803
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/jb.168.3.1291-1301.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062715363
244 rdf:type schema:CreativeWork
245 https://www.grid.ac/institutes/grid.4818.5 schema:alternateName Wageningen University & Research
246 schema:name Laboratory of Plant Breeding, Wageningen University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
247 Plant Research International, P.O. Box 16, 6700 AJ Wageningen, The Netherlands.
248 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...