siRNA-mediated gene silencing in vitro and in vivo View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-10

AUTHORS

Haibin Xia, Qinwen Mao, Henry L Paulson, Beverly L Davidson

ABSTRACT

RNA interference is now established as an important biological strategy for gene silencing, but its application to mammalian cells has been limited by nonspecific inhibitory effects of long dsRNA on translation. Here, we describe a viral-mediated delivery mechanism that results in specific silencing of targeted genes through expression of small interfering RNA (siRNA). We establish proof of principle by markedly diminishing expression of exogenous and endogenous genes in vitro and in vivo in brain and liver, and further apply this strategy to a model system of a major class of neurodegenerative disorders, the polyglutamine diseases, to show reduced polyglutamine aggregation in cells. This viral-mediated strategy should prove generally useful in reducing expression of target genes to model biological processes or to provide therapy for dominant human diseases. More... »

PAGES

1006-1010

References to SciGraph publications

Journal

TITLE

Nature Biotechnology

ISSUE

10

VOLUME

20

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt739

    DOI

    http://dx.doi.org/10.1038/nbt739

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1008960799

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12244328


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