Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-09

AUTHORS

Mark Richards, Chui-Yee Fong, Woon-Khiong Chan, Peng-Cheang Wong, Ariff Bongso

ABSTRACT

Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from the animal feeder, matrix, or conditioned medium to the HES cells, thus compromising later clinical application. Here we show that human fetal and adult fibroblast feeders support prolonged undifferentiated HES cell growth of existing cell lines and are superior to cell-free matrices (collagen I, human extracellular matrix, Matrigel, and laminin) supplemented with human or MEF feeder-conditioned medium. Additionally, we report the derivation and establishment of a new HES cell line in completely animal-free conditions. Like HES cells cultured on MEF feeders, the HES cells grown on human feeders had normal karyotypes, tested positive for alkaline phosphatase activity, expressed Oct-4 and cell surface markers including SSEA-3, SSEA-4, Tra 1-60, and GCTM-2, formed teratomas in severely combined immunodeficient (SCID) mice, and retained all key morphological characteristics. Human feeder#150;supported HES cells should provide a safer alternative to existing HES cell lines in therapeutic applications. More... »

PAGES

933-936

Journal

TITLE

Nature Biotechnology

ISSUE

9

VOLUME

20

Author Affiliations

Related Patents

  • Culture Of Human Embryonic Stem Cells On A Matrix With Tgfb1 And Bfgf
  • Mapc Generation Of Muscle
  • Human Embryonic Stem Cells Genetically Modified To Contain A Nucleic Acid And Cultured With Fibroblast Growth Factor
  • Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Stems Cells
  • Method For Differentiating Embryonic Stem Cells Into Cells Expressing Aqp-1
  • Stem Cells
  • Artificial Mammalian Chromosome
  • Suspension Culture Of Human Embryonic Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Primate Pluripotent Stem Cell Expansion Without Feeder Cells And In The Presence Of Fgf And Matrigel Or Engelbreth-Holm-Swarm Tumor Cell Preparation
  • Stem Cells Culture Systems
  • Substrates And Methods For Culturing Stem Cells
  • Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Non-Invasive, In Vitro Functional Tissue Assay Systems
  • Assay For Drug Discovery Based On In Vitro Differentiated Cells
  • Undifferentiated Stem Cell Culture Systems
  • A Method For Obtaining A Xeno-Free Hbs Cell Line
  • Characterization And Isolation Of Subsets Of Human Embryonic Stem Cells (Hes) And Cells Associated Or Derived Therefrom
  • Isolated Embryonic-Like Stem Cells Derived From Human Umbilical Cord Blood
  • Tissue Culture Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Isolated Primate Embryonic Cells Derived From Extended Blastocysts
  • Screening Methods For Human Embryonic Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Stem Cells Culture Systems
  • Method For Screening For Pluripotent Stem Cell Growth-Promoting Factor
  • Human Foreskin Cells Suitable For Culturing Stem Cells
  • Human Foreskin Cells Suitable For Culturing Stem Cells
  • Composition And Method For Proliferating Undifferentiated Stem Cells
  • Co-Culturing Mammalian Embryonic Stem Cells With Human Foreskin Fibroblasts
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Isolation Of Cd14 Negative, Cd45 Positive And Cd117 Positive Embryonic-Like Stem Cells Free Of Monocytes From Human Umbilical Cord Blood Mononuclear Cells
  • Embryonic-Like Stem Cells Derived From Adult Human Peripheral Blood And Methods Of Use
  • Methods Of Culturing Cells In A Medium Comprising Transforming Growth Factor Beta 1 And Basic Fibroblast Growth Factor
  • Human Foreskin Cells Suitable For Culturing Stem Cells
  • Human Embryonic Stem Cells And Culturing Methods Thereof
  • Characterization And Isolation Of Subsets Of Human Embryonic Stem Cells (Hes) And Cells Associated Or Derived Therefrom
  • Artificial Mammalian Chromosome
  • Composition And Method For Enabling Proliferation Of Pluripotent Human Stem Cells
  • Human Foreskin Fibroblasts For Culturing Es Cells
  • Undifferentiated Stem Cell Culture Systems
  • Methods Of Deriving Mammalian Pluripotent Stem Cell Lines
  • Human Foreskin Fibroblast Conditioned Media For Culturing Es Cells
  • Endodermal Progenitor Cells
  • Cell Culture Medium And Culture Method Using Same
  • Culturing Mammalian Embryonic Stem Cells In Human Foreskin Fibroblast Conditioned Medium
  • Method For Evaluating Cell Populations
  • Feeder Independent Extended Culture Of Embryonic Stem Cells
  • Method For Producing Induced Pluripotent Stem Cells And Method For Culturing The Same
  • Generation Of A Lineage Specific Cell From A Primate Extended Blastocyst Cell Line
  • Method For Evaluating Cell Populations
  • Adhesive Signature-Based Methods For The Isolation Of Stem Cells And Cells Derived Therefrom
  • Micro-Carrier Culture System For Rapid Expansion Of Human Embryonic Stem Cells
  • Composition And Method For Enabling Proliferation Of Pluripotent Stem Cells
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt726

    DOI

    http://dx.doi.org/10.1038/nbt726

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009583211

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12161760


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Division", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cytoplasmic Structures", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Muscles", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Skin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cell Transplantation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "National University of Singapore", 
              "id": "https://www.grid.ac/institutes/grid.4280.e", 
              "name": [
                "Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119260."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Richards", 
            "givenName": "Mark", 
            "id": "sg:person.01101265040.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101265040.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National University of Singapore", 
              "id": "https://www.grid.ac/institutes/grid.4280.e", 
              "name": [
                "Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119260."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fong", 
            "givenName": "Chui-Yee", 
            "id": "sg:person.01042303061.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042303061.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National University of Singapore", 
              "id": "https://www.grid.ac/institutes/grid.4280.e", 
              "name": [
                "Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore 119260."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chan", 
            "givenName": "Woon-Khiong", 
            "id": "sg:person.01324277521.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324277521.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National University of Singapore", 
              "id": "https://www.grid.ac/institutes/grid.4280.e", 
              "name": [
                "Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119260."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wong", 
            "givenName": "Peng-Cheang", 
            "id": "sg:person.01171371600.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171371600.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National University of Singapore", 
              "id": "https://www.grid.ac/institutes/grid.4280.e", 
              "name": [
                "Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119260."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bongso", 
            "givenName": "Ariff", 
            "id": "sg:person.047735522.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047735522.26"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/336688a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010804374", 
              "https://doi.org/10.1038/336688a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336688a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010804374", 
              "https://doi.org/10.1038/336688a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0093-691x(05)80077-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011917255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1001-971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014515248", 
              "https://doi.org/10.1038/nbt1001-971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1001-971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014515248", 
              "https://doi.org/10.1038/nbt1001-971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/humrep/13.10.2926", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031444917"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/humrep/16.10.2187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037805910"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336684a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038677733", 
              "https://doi.org/10.1038/336684a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/336684a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038677733", 
              "https://doi.org/10.1038/336684a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/oxfordjournals.humrep.a136932", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079366738"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/oxfordjournals.humrep.a138401", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082543244"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2002-09", 
        "datePublishedReg": "2002-09-01", 
        "description": "Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from the animal feeder, matrix, or conditioned medium to the HES cells, thus compromising later clinical application. Here we show that human fetal and adult fibroblast feeders support prolonged undifferentiated HES cell growth of existing cell lines and are superior to cell-free matrices (collagen I, human extracellular matrix, Matrigel, and laminin) supplemented with human or MEF feeder-conditioned medium. Additionally, we report the derivation and establishment of a new HES cell line in completely animal-free conditions. Like HES cells cultured on MEF feeders, the HES cells grown on human feeders had normal karyotypes, tested positive for alkaline phosphatase activity, expressed Oct-4 and cell surface markers including SSEA-3, SSEA-4, Tra 1-60, and GCTM-2, formed teratomas in severely combined immunodeficient (SCID) mice, and retained all key morphological characteristics. Human feeder#150;supported HES cells should provide a safer alternative to existing HES cell lines in therapeutic applications.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt726", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "9", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "20"
          }
        ], 
        "name": "Human feeders support prolonged undifferentiated growth of human inner\n\t\tcell masses and embryonic stem cells", 
        "pagination": "933-936", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "2da6dcd36eae4406c63ccb8356d8885f4993c0baf1172c58d558042cf71363be"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "12161760"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt726"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1009583211"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt726", 
          "https://app.dimensions.ai/details/publication/pub.1009583211"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:22", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87088_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt726"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt726'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt726'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt726'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt726'


     

    This table displays all metadata directly associated to this object as RDF triples.

    188 TRIPLES      21 PREDICATES      53 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt726 schema:about N0cd3fe19d4624bceaf3f85e4d9cf7edd
    2 N6477a0369e4e4c8c86d0cb602b95f8e1
    3 N6c3018d2795b439287710dbe60c1b9b3
    4 N723e5301da24409e9c53419bab26b01b
    5 N7fd4bb414fc541a19a77a3836fb9b7bc
    6 N87cf579cf53b4623a7f914c75fd926ee
    7 N87e3439b23654220a439427a658121c5
    8 N912e286dfc774214b2b9483e4f099431
    9 Nad5e0ad38ec1411a93d56d4d7c982397
    10 Nb0c3edafa8854789b8f219f792a7478c
    11 Nb72a02f4d6d0405e8b7b7fdb74196c23
    12 Nb84c20f46d364d278708ac8c589ddb1d
    13 Ne405078274734947b942ca346d32c7d1
    14 Nf78a029a99484986b51880ac0d49a045
    15 anzsrc-for:06
    16 anzsrc-for:0601
    17 schema:author N01ded34cdea9435a80084e35146d61a8
    18 schema:citation sg:pub.10.1038/336684a0
    19 sg:pub.10.1038/336688a0
    20 sg:pub.10.1038/74447
    21 sg:pub.10.1038/nbt1001-971
    22 https://doi.org/10.1016/s0093-691x(05)80077-9
    23 https://doi.org/10.1093/humrep/13.10.2926
    24 https://doi.org/10.1093/humrep/16.10.2187
    25 https://doi.org/10.1093/oxfordjournals.humrep.a136932
    26 https://doi.org/10.1093/oxfordjournals.humrep.a138401
    27 https://doi.org/10.1126/science.282.5391.1145
    28 schema:datePublished 2002-09
    29 schema:datePublishedReg 2002-09-01
    30 schema:description Previous reports have demonstrated the growth of undifferentiated human embryonic stem (HES) cells on mouse embryonic fibroblast (MEF) feeders and on laminin- or Matrigel-coated plastic surfaces supplemented with MEF-conditioned medium. These xenosupport systems run the risk of cross-transfer of animal pathogens from the animal feeder, matrix, or conditioned medium to the HES cells, thus compromising later clinical application. Here we show that human fetal and adult fibroblast feeders support prolonged undifferentiated HES cell growth of existing cell lines and are superior to cell-free matrices (collagen I, human extracellular matrix, Matrigel, and laminin) supplemented with human or MEF feeder-conditioned medium. Additionally, we report the derivation and establishment of a new HES cell line in completely animal-free conditions. Like HES cells cultured on MEF feeders, the HES cells grown on human feeders had normal karyotypes, tested positive for alkaline phosphatase activity, expressed Oct-4 and cell surface markers including SSEA-3, SSEA-4, Tra 1-60, and GCTM-2, formed teratomas in severely combined immunodeficient (SCID) mice, and retained all key morphological characteristics. Human feeder#150;supported HES cells should provide a safer alternative to existing HES cell lines in therapeutic applications.
    31 schema:genre research_article
    32 schema:inLanguage en
    33 schema:isAccessibleForFree false
    34 schema:isPartOf N549a5d5cff1e4e9c9254d16a6e1d1aa0
    35 N56785317e01f45ba9e2cb708864490a0
    36 sg:journal.1115214
    37 schema:name Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells
    38 schema:pagination 933-936
    39 schema:productId N62973c67c4cd4cef9f7418356b6308e1
    40 N73caa41d35254984801e5947f7d02301
    41 N7fda5f99f1904d14a29236bf8a333be6
    42 N8d201cae96964cb1aed2701f6f1b47a0
    43 N91afb078625942998e567b5e0fe6f7b7
    44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009583211
    45 https://doi.org/10.1038/nbt726
    46 schema:sdDatePublished 2019-04-11T12:22
    47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    48 schema:sdPublisher N456693765b2040f38c6ed165ab6ea75d
    49 schema:url http://www.nature.com/articles/nbt726
    50 sgo:license sg:explorer/license/
    51 sgo:sdDataset articles
    52 rdf:type schema:ScholarlyArticle
    53 N01ded34cdea9435a80084e35146d61a8 rdf:first sg:person.01101265040.13
    54 rdf:rest N305507f7ddb54831b28d8affade71be8
    55 N0cd3fe19d4624bceaf3f85e4d9cf7edd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    56 schema:name Cell Line
    57 rdf:type schema:DefinedTerm
    58 N2555df2f973a4995b3a3bbfcb774947f rdf:first sg:person.01324277521.89
    59 rdf:rest Ne9e8b8460cb14128a6cb485754d6488c
    60 N305507f7ddb54831b28d8affade71be8 rdf:first sg:person.01042303061.09
    61 rdf:rest N2555df2f973a4995b3a3bbfcb774947f
    62 N3e3611c2585149bd9f426a621653dd39 rdf:first sg:person.047735522.26
    63 rdf:rest rdf:nil
    64 N456693765b2040f38c6ed165ab6ea75d schema:name Springer Nature - SN SciGraph project
    65 rdf:type schema:Organization
    66 N549a5d5cff1e4e9c9254d16a6e1d1aa0 schema:volumeNumber 20
    67 rdf:type schema:PublicationVolume
    68 N56785317e01f45ba9e2cb708864490a0 schema:issueNumber 9
    69 rdf:type schema:PublicationIssue
    70 N62973c67c4cd4cef9f7418356b6308e1 schema:name dimensions_id
    71 schema:value pub.1009583211
    72 rdf:type schema:PropertyValue
    73 N6477a0369e4e4c8c86d0cb602b95f8e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Fibroblasts
    75 rdf:type schema:DefinedTerm
    76 N6c3018d2795b439287710dbe60c1b9b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Mice
    78 rdf:type schema:DefinedTerm
    79 N723e5301da24409e9c53419bab26b01b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Humans
    81 rdf:type schema:DefinedTerm
    82 N73caa41d35254984801e5947f7d02301 schema:name readcube_id
    83 schema:value 2da6dcd36eae4406c63ccb8356d8885f4993c0baf1172c58d558042cf71363be
    84 rdf:type schema:PropertyValue
    85 N7fd4bb414fc541a19a77a3836fb9b7bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Animals
    87 rdf:type schema:DefinedTerm
    88 N7fda5f99f1904d14a29236bf8a333be6 schema:name doi
    89 schema:value 10.1038/nbt726
    90 rdf:type schema:PropertyValue
    91 N87cf579cf53b4623a7f914c75fd926ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name Cell Culture Techniques
    93 rdf:type schema:DefinedTerm
    94 N87e3439b23654220a439427a658121c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Stem Cell Transplantation
    96 rdf:type schema:DefinedTerm
    97 N8d201cae96964cb1aed2701f6f1b47a0 schema:name pubmed_id
    98 schema:value 12161760
    99 rdf:type schema:PropertyValue
    100 N912e286dfc774214b2b9483e4f099431 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Cell Division
    102 rdf:type schema:DefinedTerm
    103 N91afb078625942998e567b5e0fe6f7b7 schema:name nlm_unique_id
    104 schema:value 9604648
    105 rdf:type schema:PropertyValue
    106 Nad5e0ad38ec1411a93d56d4d7c982397 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Skin
    108 rdf:type schema:DefinedTerm
    109 Nb0c3edafa8854789b8f219f792a7478c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Cell Differentiation
    111 rdf:type schema:DefinedTerm
    112 Nb72a02f4d6d0405e8b7b7fdb74196c23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Muscles
    114 rdf:type schema:DefinedTerm
    115 Nb84c20f46d364d278708ac8c589ddb1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Epithelial Cells
    117 rdf:type schema:DefinedTerm
    118 Ne405078274734947b942ca346d32c7d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Stem Cells
    120 rdf:type schema:DefinedTerm
    121 Ne9e8b8460cb14128a6cb485754d6488c rdf:first sg:person.01171371600.35
    122 rdf:rest N3e3611c2585149bd9f426a621653dd39
    123 Nf78a029a99484986b51880ac0d49a045 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Cytoplasmic Structures
    125 rdf:type schema:DefinedTerm
    126 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    127 schema:name Biological Sciences
    128 rdf:type schema:DefinedTerm
    129 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    130 schema:name Biochemistry and Cell Biology
    131 rdf:type schema:DefinedTerm
    132 sg:journal.1115214 schema:issn 1087-0156
    133 1546-1696
    134 schema:name Nature Biotechnology
    135 rdf:type schema:Periodical
    136 sg:person.01042303061.09 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
    137 schema:familyName Fong
    138 schema:givenName Chui-Yee
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042303061.09
    140 rdf:type schema:Person
    141 sg:person.01101265040.13 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
    142 schema:familyName Richards
    143 schema:givenName Mark
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101265040.13
    145 rdf:type schema:Person
    146 sg:person.01171371600.35 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
    147 schema:familyName Wong
    148 schema:givenName Peng-Cheang
    149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171371600.35
    150 rdf:type schema:Person
    151 sg:person.01324277521.89 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
    152 schema:familyName Chan
    153 schema:givenName Woon-Khiong
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324277521.89
    155 rdf:type schema:Person
    156 sg:person.047735522.26 schema:affiliation https://www.grid.ac/institutes/grid.4280.e
    157 schema:familyName Bongso
    158 schema:givenName Ariff
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.047735522.26
    160 rdf:type schema:Person
    161 sg:pub.10.1038/336684a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038677733
    162 https://doi.org/10.1038/336684a0
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1038/336688a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010804374
    165 https://doi.org/10.1038/336688a0
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1038/74447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033604670
    168 https://doi.org/10.1038/74447
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1038/nbt1001-971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014515248
    171 https://doi.org/10.1038/nbt1001-971
    172 rdf:type schema:CreativeWork
    173 https://doi.org/10.1016/s0093-691x(05)80077-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011917255
    174 rdf:type schema:CreativeWork
    175 https://doi.org/10.1093/humrep/13.10.2926 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031444917
    176 rdf:type schema:CreativeWork
    177 https://doi.org/10.1093/humrep/16.10.2187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037805910
    178 rdf:type schema:CreativeWork
    179 https://doi.org/10.1093/oxfordjournals.humrep.a136932 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079366738
    180 rdf:type schema:CreativeWork
    181 https://doi.org/10.1093/oxfordjournals.humrep.a138401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082543244
    182 rdf:type schema:CreativeWork
    183 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    184 rdf:type schema:CreativeWork
    185 https://www.grid.ac/institutes/grid.4280.e schema:alternateName National University of Singapore
    186 schema:name Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore 119260.
    187 Department of Obstetrics and Gynaecology, National University of Singapore, Kent Ridge, Singapore 119260.
    188 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...