Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-06-29

AUTHORS

Elena E Perez, Jianbin Wang, Jeffrey C Miller, Yann Jouvenot, Kenneth A Kim, Olga Liu, Nathaniel Wang, Gary Lee, Victor V Bartsevich, Ya-Li Lee, Dmitry Y Guschin, Igor Rupniewski, Adam J Waite, Carmine Carpenito, Richard G Carroll, Jordan S Orange, Fyodor D Urnov, Edward J Rebar, Dale Ando, Philip D Gregory, James L Riley, Michael C Holmes, Carl H June

ABSTRACT

Homozygosity for the naturally occurring Δ32 deletion in the HIV co-receptor CCR5 confers resistance to HIV-1 infection. We generated an HIV-resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5. Transient expression of CCR5 ZFNs permanently and specifically disrupted ∼50% of CCR5 alleles in a pool of primary human CD4+ T cells. Genetic disruption of CCR5 provided robust, stable and heritable protection against HIV-1 infection in vitro and in vivo in a NOG model of HIV infection. HIV-1-infected mice engrafted with ZFN-modified CD4+ T cells had lower viral loads and higher CD4+ T-cell counts than mice engrafted with wild-type CD4+ T cells, consistent with the potential to reconstitute immune function in individuals with HIV/AIDS by maintenance of an HIV-resistant CD4+ T-cell population. Thus adoptive transfer of ex vivo expanded CCR5 ZFN–modified autologous CD4+ T cells in HIV patients is an attractive approach for the treatment of HIV-1 infection. More... »

PAGES

808-816

References to SciGraph publications

  • 2003-08-28. Regulation and mechanisms of mammalian double-strand break repair in ONCOGENE
  • 2005-11-20. Host-reactive CD8+ memory stem cells in graft-versus-host disease in NATURE MEDICINE
  • 1999-10. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions in NATURE
  • 2001-07. A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter in NATURE BIOTECHNOLOGY
  • 2005-04-03. Highly efficient endogenous human gene correction using designed zinc-finger nucleases in NATURE
  • 2007-10-28. Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery in NATURE BIOTECHNOLOGY
  • 2005-11. Chromatin remodelling at a DNA double-strand break site in Saccharomyces cerevisiae in NATURE
  • 2002-01. Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreased CCR5 expression in HIV infection in NATURE MEDICINE
  • 2005-07-31. Genome sequencing in microfabricated high-density picolitre reactors in NATURE
  • 1996-11. The role of a mutant CCR5 allele in HIV–1 transmission and disease progression in NATURE MEDICINE
  • 1996-06. Identification of a major co-receptor for primary isolates of HIV-1 in NATURE
  • 2005-10-05. Efficacy of short-term monotherapy with maraviroc, a new CCR5 antagonist, in patients infected with HIV-1 in NATURE MEDICINE
  • 1997-10. CCR2 chemokine receptor and AIDS progression in NATURE MEDICINE
  • 2007-07-01. An improved zinc-finger nuclease architecture for highly specific genome editing in NATURE BIOTECHNOLOGY
  • 2003-05. Drug discovery with engineered zinc-finger proteins in NATURE REVIEWS DRUG DISCOVERY
  • 2007-12-07. Genetic therapies against HIV in NATURE BIOTECHNOLOGY
  • 1996-08. Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene in NATURE
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    7

    VOLUME

    26

    Related Patents

  • In Vivo Determination Of The Lokalization Of Dna Double-Stand Breaks And Application Thereof
  • Aptamer-Mrna Conjugates For Targeted Protein Or Peptide Expression And Methods For Their Use
  • New Sequence Specific Reagents Targeting Ccr5 In Primary Hematopoietic Cells
  • Modification Of Cxcr4 Using Engineered Zinc Finger Proteins
  • Methods And Compositions For Gene Inactivation
  • Dna-Binding Proteins And Uses Thereof
  • Targeted Genomic Modification With Partially Single-Stranded Donor Molecules
  • Methods And Compositions For Increasing Transgene Activity
  • Methods And Compositions For Treating Huntington's Disease
  • Genome Editing Using Targeting Endonucleases And Single-Stranded Nucleic Acids
  • Determination Of In Vivo Dna Double-Strand Break Localization And Application Thereof
  • Determination Of In Vivo Dna Double-Strand Break Localization And Application Thereof
  • Methods And Compostions For Targeted Genomic Deletion
  • Rapid Screening Of Biologically Active Nucleases And Isolation Of Nuclease-Modified Cells
  • Rapid Screening Of Biologically Active Nucleases And Isolation Of Nuclease-Modified Cells
  • Methods And Compositions For Gene Correction
  • Methods And Compositions For Increasing Nuclease Activity
  • Methods And Compositions For Gene Inactivation
  • Switchable Cas9 Nucleases And Uses Thereof
  • T-Cell Specific Genome Editing
  • Methods And Compositions For Generating Or Maintaining Pluripotent Cells
  • Genome Editing Methods
  • Nucleotide-Specific Recognition Sequences For Designer Tal Effectors
  • Nuclease Fusions For Enhancing Genome Editing By Homology-Directed Transgene Integration
  • Methods And Compositions For Nuclease Design
  • Crispr/Cas-Related Methods And Compositions For Treating Leber's Congenital Amaurosis 10 (Lca10)
  • Nuclease-Mediated Targeting With Large Targeting Vectors
  • Dna-Binding Proteins And Uses Thereof
  • Arrdc1-Mediated Microvesicles (Armms) And Uses Thereof
  • Methods And Compositions For Targeted Genomic Deletion
  • Dna-Binding Proteins And Uses Thereof
  • Polynucleotides Encoding A Human Trim-Cyp Fusion Polypeptide, Compositions Thereof, And Methods Of Using Same
  • Methods And Compositions For Modification Of The Hprt Locus
  • Methods And Compositions For Treating Trinucleotide Repeat Disorders
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Aptamer-Mrna Conjugates For Targeted Protein Or Peptide Expression And Methods For Their Use
  • Dna-Binding Proteins And Uses Thereof
  • Modification Of Cxcr4 Using Engineered Zinc Finger Proteins
  • Evaluation And Improvement Of Nuclease Cleavage Specificity
  • Gene Correction Of Scid-Related Genes In Hematopoietic Stem And Progenitor Cells
  • Genetic Correction Of Mutated Genes
  • Methods And Compositions For Gene Correction
  • Methods And Compositions For Increasing Nuclease Activity
  • Aptamer-Mrna Conjugates For Targeted Protein Or Peptide Expression And Methods For Their Use
  • Methods And Compositions For Modification Of The Hprt Locus
  • Engineered Zinc Finger Proteins For Treating Trinucleotide Repeat Disorders
  • Modified Dna-Binding Proteins And Uses Thereof
  • Method For The Monitoring Of Modified Nucleases Induced-Gene Editing Events By Molecular Combing
  • Targeted Genomic Modification With Partially Single-Stranded Donor Molecules
  • Use Of Endogenous Promoters To Express Heterologous Proteins
  • Functional Genomics Using Crispr-Cas Systems, Compositions, Methods, Knock Out Libraries And Applications Thereof
  • Crispr/Cas-Related Methods And Compositions For Treating Leber's Congenital Amaurosis 10 (Lca10)
  • Methods And Compositions For Prevention Or Treatment Of A Disease
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1410

    DOI

    http://dx.doi.org/10.1038/nbt1410

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048577624

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18587387


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adoptive Transfer", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CD4-Positive T-Lymphocytes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromosome Mapping", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Deoxyribonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HIV Infections", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Immunity, Innate", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Treatment Outcome", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Zinc Fingers", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA.", 
              "id": "http://www.grid.ac/institutes/grid.239552.a", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
                "Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Perez", 
            "givenName": "Elena E", 
            "id": "sg:person.0607221441.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607221441.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Jianbin", 
            "id": "sg:person.011354434034.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011354434034.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Miller", 
            "givenName": "Jeffrey C", 
            "id": "sg:person.01174076271.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174076271.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Present address: Process Science Department, Bayer Hematology/Cardiology, 800 Dwight Way, Berkeley, California 94701, USA.", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
                "Present address: Process Science Department, Bayer Hematology/Cardiology, 800 Dwight Way, Berkeley, California 94701, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jouvenot", 
            "givenName": "Yann", 
            "id": "sg:person.0771563241.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771563241.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Kenneth A", 
            "id": "sg:person.01203760001.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203760001.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "Olga", 
            "id": "sg:person.01106011641.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106011641.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Nathaniel", 
            "id": "sg:person.0753123553.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753123553.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Gary", 
            "id": "sg:person.01010776354.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010776354.96"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bartsevich", 
            "givenName": "Victor V", 
            "id": "sg:person.01270353441.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270353441.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Ya-Li", 
            "id": "sg:person.01336466641.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336466641.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guschin", 
            "givenName": "Dmitry Y", 
            "id": "sg:person.01057647671.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057647671.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rupniewski", 
            "givenName": "Igor", 
            "id": "sg:person.0661175741.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661175741.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Waite", 
            "givenName": "Adam J", 
            "id": "sg:person.0725177232.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725177232.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Carpenito", 
            "givenName": "Carmine", 
            "id": "sg:person.0732060266.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732060266.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Carroll", 
            "givenName": "Richard G", 
            "id": "sg:person.010256663157.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010256663157.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA.", 
              "id": "http://www.grid.ac/institutes/grid.239552.a", 
              "name": [
                "Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "S Orange", 
            "givenName": "Jordan", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Urnov", 
            "givenName": "Fyodor D", 
            "id": "sg:person.01065115346.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065115346.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rebar", 
            "givenName": "Edward J", 
            "id": "sg:person.01025207376.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025207376.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ando", 
            "givenName": "Dale", 
            "id": "sg:person.0645571071.11", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645571071.11"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gregory", 
            "givenName": "Philip D", 
            "id": "sg:person.015620210017.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015620210017.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Riley", 
            "givenName": "James L", 
            "id": "sg:person.0770005773.92", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770005773.92"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
              "id": "http://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Holmes", 
            "givenName": "Michael C", 
            "id": "sg:person.01011534471.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011534471.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.", 
              "id": "http://www.grid.ac/institutes/grid.25879.31", 
              "name": [
                "Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "June", 
            "givenName": "Carl H", 
            "id": "sg:person.014235534277.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014235534277.63"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nrd1087", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040287926", 
              "https://doi.org/10.1038/nrd1087"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/381661a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053562821", 
              "https://doi.org/10.1038/381661a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044521333", 
              "https://doi.org/10.1038/nature04148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041122840", 
              "https://doi.org/10.1038/nm1319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1326", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024397965", 
              "https://doi.org/10.1038/nm1326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040117781", 
              "https://doi.org/10.1038/nature03556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/90264", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032644172", 
              "https://doi.org/10.1038/90264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/44385", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016546162", 
              "https://doi.org/10.1038/44385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm0102-47", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018873794", 
              "https://doi.org/10.1038/nm0102-47"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002154526", 
              "https://doi.org/10.1038/nbt1319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03959", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021574562", 
              "https://doi.org/10.1038/nature03959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1097-1052c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051084444", 
              "https://doi.org/10.1038/nm1097-1052c"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1206679", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024607188", 
              "https://doi.org/10.1038/sj.onc.1206679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1367", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008151467", 
              "https://doi.org/10.1038/nbt1367"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/382722a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050488632", 
              "https://doi.org/10.1038/382722a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1353", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026122095", 
              "https://doi.org/10.1038/nbt1353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1196-1240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026944069", 
              "https://doi.org/10.1038/nm1196-1240"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2008-06-29", 
        "datePublishedReg": "2008-06-29", 
        "description": "Homozygosity for the naturally occurring \u039432 deletion in the HIV co-receptor CCR5 confers resistance to HIV-1 infection. We generated an HIV-resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5. Transient expression of CCR5 ZFNs permanently and specifically disrupted \u223c50% of CCR5 alleles in a pool of primary human CD4+ T cells. Genetic disruption of CCR5 provided robust, stable and heritable protection against HIV-1 infection in vitro and in vivo in a NOG model of HIV infection. HIV-1-infected mice engrafted with ZFN-modified CD4+ T cells had lower viral loads and higher CD4+ T-cell counts than mice engrafted with wild-type CD4+ T cells, consistent with the potential to reconstitute immune function in individuals with HIV/AIDS by maintenance of an HIV-resistant CD4+ T-cell population. Thus adoptive transfer of ex vivo expanded CCR5 ZFN\u2013modified autologous CD4+ T cells in HIV patients is an attractive approach for the treatment of HIV-1 infection.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/nbt1410", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2410087", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2695835", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2684826", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "26"
          }
        ], 
        "keywords": [
          "HIV-1 infection", 
          "HIV-1-infected mice", 
          "HIV co-receptor CCR5", 
          "low viral load", 
          "HIV-1 resistance", 
          "co-receptor CCR5", 
          "HIV/AIDS", 
          "primary human CD4", 
          "wild-type CD4", 
          "\u039432 deletion", 
          "higher CD4", 
          "autologous CD4", 
          "HIV patients", 
          "adoptive transfer", 
          "HIV infection", 
          "viral load", 
          "CCR5 allele", 
          "human CD4", 
          "CD4", 
          "immune function", 
          "cell count", 
          "ex vivo", 
          "CCR5", 
          "infection", 
          "cell populations", 
          "genetic disruption", 
          "mice", 
          "cells", 
          "vivo", 
          "de novo", 
          "patients", 
          "zinc finger nucleases", 
          "AIDS", 
          "treatment", 
          "attractive approach", 
          "vitro", 
          "transient expression", 
          "count", 
          "homozygosity", 
          "resistance", 
          "expression", 
          "population", 
          "individuals", 
          "disruption", 
          "novo", 
          "alleles", 
          "deletion", 
          "maintenance", 
          "protection", 
          "function", 
          "pool", 
          "potential", 
          "nuclease", 
          "establishment", 
          "model", 
          "approach", 
          "editing", 
          "load", 
          "transfer", 
          "HIV-resistant genotype de novo", 
          "genotype de novo", 
          "endogenous CCR5", 
          "CCR5 ZFNs", 
          "heritable protection", 
          "NOG model", 
          "HIV-resistant CD4"
        ], 
        "name": "Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases", 
        "pagination": "808-816", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1048577624"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1410"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "18587387"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1410", 
          "https://app.dimensions.ai/details/publication/pub.1048577624"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-11-01T18:11", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_454.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/nbt1410"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1410'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1410'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1410'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1410'


     

    This table displays all metadata directly associated to this object as RDF triples.

    422 TRIPLES      22 PREDICATES      123 URIs      97 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1410 schema:about N00089f9bc9f3479c9f67e0d3785d39f3
    2 N0783d231384540e0be7d522736987cc5
    3 N20850fe30b2c41f49c7eff08f4de78e9
    4 N2bed55c899d84b3e8a745b4c87d0c642
    5 N2fe77e7930da42bf920fb325cd31edde
    6 N365faee3873e486ea3876cd6732dc3f6
    7 N774b902aee394d84a376298e5128e62a
    8 N86b0139c779f4643979cbb3785214ca1
    9 N8fb66b7d890d4b5e95e94b07731d28c8
    10 N952cc2762bfb47e493071470d322886f
    11 Nc54fe646cea14887be1b9400f1097496
    12 Nde8ec08af9da424aa734b92458e1dd13
    13 Nfa18a7951b6540c8b1bc12cfc291abd3
    14 anzsrc-for:11
    15 anzsrc-for:1107
    16 anzsrc-for:1108
    17 schema:author Nfc8e0f8ca04d47bb8e9e33493a7af30f
    18 schema:citation sg:pub.10.1038/381661a0
    19 sg:pub.10.1038/382722a0
    20 sg:pub.10.1038/44385
    21 sg:pub.10.1038/90264
    22 sg:pub.10.1038/nature03556
    23 sg:pub.10.1038/nature03959
    24 sg:pub.10.1038/nature04148
    25 sg:pub.10.1038/nbt1319
    26 sg:pub.10.1038/nbt1353
    27 sg:pub.10.1038/nbt1367
    28 sg:pub.10.1038/nm0102-47
    29 sg:pub.10.1038/nm1097-1052c
    30 sg:pub.10.1038/nm1196-1240
    31 sg:pub.10.1038/nm1319
    32 sg:pub.10.1038/nm1326
    33 sg:pub.10.1038/nrd1087
    34 sg:pub.10.1038/sj.onc.1206679
    35 schema:datePublished 2008-06-29
    36 schema:datePublishedReg 2008-06-29
    37 schema:description Homozygosity for the naturally occurring Δ32 deletion in the HIV co-receptor CCR5 confers resistance to HIV-1 infection. We generated an HIV-resistant genotype de novo using engineered zinc-finger nucleases (ZFNs) to disrupt endogenous CCR5. Transient expression of CCR5 ZFNs permanently and specifically disrupted ∼50% of CCR5 alleles in a pool of primary human CD4+ T cells. Genetic disruption of CCR5 provided robust, stable and heritable protection against HIV-1 infection in vitro and in vivo in a NOG model of HIV infection. HIV-1-infected mice engrafted with ZFN-modified CD4+ T cells had lower viral loads and higher CD4+ T-cell counts than mice engrafted with wild-type CD4+ T cells, consistent with the potential to reconstitute immune function in individuals with HIV/AIDS by maintenance of an HIV-resistant CD4+ T-cell population. Thus adoptive transfer of ex vivo expanded CCR5 ZFN–modified autologous CD4+ T cells in HIV patients is an attractive approach for the treatment of HIV-1 infection.
    38 schema:genre article
    39 schema:inLanguage en
    40 schema:isAccessibleForFree true
    41 schema:isPartOf Na56f5512625841e99c9c537b5cb7a98e
    42 Nd91bb31dd5e343c3b3bacf211a1a446b
    43 sg:journal.1115214
    44 schema:keywords AIDS
    45 CCR5
    46 CCR5 ZFNs
    47 CCR5 allele
    48 CD4
    49 HIV co-receptor CCR5
    50 HIV infection
    51 HIV patients
    52 HIV-1 infection
    53 HIV-1 resistance
    54 HIV-1-infected mice
    55 HIV-resistant CD4
    56 HIV-resistant genotype de novo
    57 HIV/AIDS
    58 NOG model
    59 adoptive transfer
    60 alleles
    61 approach
    62 attractive approach
    63 autologous CD4
    64 cell count
    65 cell populations
    66 cells
    67 co-receptor CCR5
    68 count
    69 de novo
    70 deletion
    71 disruption
    72 editing
    73 endogenous CCR5
    74 establishment
    75 ex vivo
    76 expression
    77 function
    78 genetic disruption
    79 genotype de novo
    80 heritable protection
    81 higher CD4
    82 homozygosity
    83 human CD4
    84 immune function
    85 individuals
    86 infection
    87 load
    88 low viral load
    89 maintenance
    90 mice
    91 model
    92 novo
    93 nuclease
    94 patients
    95 pool
    96 population
    97 potential
    98 primary human CD4
    99 protection
    100 resistance
    101 transfer
    102 transient expression
    103 treatment
    104 viral load
    105 vitro
    106 vivo
    107 wild-type CD4
    108 zinc finger nucleases
    109 Δ32 deletion
    110 schema:name Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
    111 schema:pagination 808-816
    112 schema:productId N0d0091f2bc1a4f369a857b1f1fe631d3
    113 N2228388815a24eaeb6b58bf203fc50cc
    114 N7d05027a3402423f83bf52cd72fef829
    115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048577624
    116 https://doi.org/10.1038/nbt1410
    117 schema:sdDatePublished 2021-11-01T18:11
    118 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    119 schema:sdPublisher N105ad24f711c44e5a4858e2168ea94b4
    120 schema:url https://doi.org/10.1038/nbt1410
    121 sgo:license sg:explorer/license/
    122 sgo:sdDataset articles
    123 rdf:type schema:ScholarlyArticle
    124 N00089f9bc9f3479c9f67e0d3785d39f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Chromosome Mapping
    126 rdf:type schema:DefinedTerm
    127 N0783d231384540e0be7d522736987cc5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Mice
    129 rdf:type schema:DefinedTerm
    130 N0c8bbb901ac44049813a138ffef43a52 rdf:first sg:person.010256663157.73
    131 rdf:rest Ndd7e8b2d91da4c529c643920c3283512
    132 N0d0091f2bc1a4f369a857b1f1fe631d3 schema:name doi
    133 schema:value 10.1038/nbt1410
    134 rdf:type schema:PropertyValue
    135 N105ad24f711c44e5a4858e2168ea94b4 schema:name Springer Nature - SN SciGraph project
    136 rdf:type schema:Organization
    137 N20850fe30b2c41f49c7eff08f4de78e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Adoptive Transfer
    139 rdf:type schema:DefinedTerm
    140 N2228388815a24eaeb6b58bf203fc50cc schema:name dimensions_id
    141 schema:value pub.1048577624
    142 rdf:type schema:PropertyValue
    143 N2bed55c899d84b3e8a745b4c87d0c642 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Deoxyribonucleases
    145 rdf:type schema:DefinedTerm
    146 N2fe77e7930da42bf920fb325cd31edde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Genetic Engineering
    148 rdf:type schema:DefinedTerm
    149 N32df4c78e4974893bbacb13210fbece7 rdf:first sg:person.0645571071.11
    150 rdf:rest N6484051081e34da99660f775c294fa03
    151 N365faee3873e486ea3876cd6732dc3f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name HIV Infections
    153 rdf:type schema:DefinedTerm
    154 N3adeb3f9daff4dc39e3733fcfe9c8df0 rdf:first sg:person.01336466641.02
    155 rdf:rest Nd67420d1efe542cca20330ab5d1ab746
    156 N40397fefc01048e4bb9beeb1c38fb355 rdf:first sg:person.01011534471.09
    157 rdf:rest Na8727e977a9a400fafd7317a44a009f1
    158 N41a1df0b25234e788537fcde0f124d8c rdf:first sg:person.01010776354.96
    159 rdf:rest N9c63206818a94506b4c03b26f816944e
    160 N5399589dedff4aaa95dab88a11f1d12e rdf:first sg:person.0725177232.35
    161 rdf:rest Nd425606be50843b19a213be8a5d9a9e5
    162 N5a917b4d682c4c79bb2194c4527f9edb rdf:first sg:person.01106011641.25
    163 rdf:rest Na9e099a568a94bd79aaa3f8308e3072c
    164 N616314d3471047fe8b3ee3a3f50bea14 rdf:first sg:person.0771563241.19
    165 rdf:rest N9e07b8f3b7734071a41e19f180b0d5ca
    166 N6484051081e34da99660f775c294fa03 rdf:first sg:person.015620210017.02
    167 rdf:rest Nac7163bdc96d4335ba1811eeb8d34131
    168 N715cf95ad98949fa8e6b2101692a7a16 rdf:first sg:person.011354434034.56
    169 rdf:rest N981046534b37414c8de01b970f382f42
    170 N774b902aee394d84a376298e5128e62a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Zinc Fingers
    172 rdf:type schema:DefinedTerm
    173 N7d05027a3402423f83bf52cd72fef829 schema:name pubmed_id
    174 schema:value 18587387
    175 rdf:type schema:PropertyValue
    176 N86b0139c779f4643979cbb3785214ca1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Immunity, Innate
    178 rdf:type schema:DefinedTerm
    179 N8fb66b7d890d4b5e95e94b07731d28c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Animals
    181 rdf:type schema:DefinedTerm
    182 N952cc2762bfb47e493071470d322886f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Treatment Outcome
    184 rdf:type schema:DefinedTerm
    185 N981046534b37414c8de01b970f382f42 rdf:first sg:person.01174076271.35
    186 rdf:rest N616314d3471047fe8b3ee3a3f50bea14
    187 N9c63206818a94506b4c03b26f816944e rdf:first sg:person.01270353441.36
    188 rdf:rest N3adeb3f9daff4dc39e3733fcfe9c8df0
    189 N9e07b8f3b7734071a41e19f180b0d5ca rdf:first sg:person.01203760001.73
    190 rdf:rest N5a917b4d682c4c79bb2194c4527f9edb
    191 Na56f5512625841e99c9c537b5cb7a98e schema:volumeNumber 26
    192 rdf:type schema:PublicationVolume
    193 Na8727e977a9a400fafd7317a44a009f1 rdf:first sg:person.014235534277.63
    194 rdf:rest rdf:nil
    195 Na9e099a568a94bd79aaa3f8308e3072c rdf:first sg:person.0753123553.37
    196 rdf:rest N41a1df0b25234e788537fcde0f124d8c
    197 Nac7163bdc96d4335ba1811eeb8d34131 rdf:first sg:person.0770005773.92
    198 rdf:rest N40397fefc01048e4bb9beeb1c38fb355
    199 Nbfef3c513e3e4c52aec7c72d6fbecef1 rdf:first sg:person.01065115346.16
    200 rdf:rest Neb1957601b6340f9a635fdad29d1b76a
    201 Nc54fe646cea14887be1b9400f1097496 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    202 schema:name Cells, Cultured
    203 rdf:type schema:DefinedTerm
    204 Nccab2c74ae0b4277a8d3b83fb43829f9 rdf:first sg:person.0661175741.04
    205 rdf:rest N5399589dedff4aaa95dab88a11f1d12e
    206 Nd425606be50843b19a213be8a5d9a9e5 rdf:first sg:person.0732060266.84
    207 rdf:rest N0c8bbb901ac44049813a138ffef43a52
    208 Nd67420d1efe542cca20330ab5d1ab746 rdf:first sg:person.01057647671.77
    209 rdf:rest Nccab2c74ae0b4277a8d3b83fb43829f9
    210 Nd91bb31dd5e343c3b3bacf211a1a446b schema:issueNumber 7
    211 rdf:type schema:PublicationIssue
    212 Ndd7e8b2d91da4c529c643920c3283512 rdf:first Nf242e16955ed4000862d4c57caab1577
    213 rdf:rest Nbfef3c513e3e4c52aec7c72d6fbecef1
    214 Nde8ec08af9da424aa734b92458e1dd13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    215 schema:name Humans
    216 rdf:type schema:DefinedTerm
    217 Neb1957601b6340f9a635fdad29d1b76a rdf:first sg:person.01025207376.70
    218 rdf:rest N32df4c78e4974893bbacb13210fbece7
    219 Nf242e16955ed4000862d4c57caab1577 schema:affiliation grid-institutes:grid.239552.a
    220 schema:familyName S Orange
    221 schema:givenName Jordan
    222 rdf:type schema:Person
    223 Nfa18a7951b6540c8b1bc12cfc291abd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    224 schema:name CD4-Positive T-Lymphocytes
    225 rdf:type schema:DefinedTerm
    226 Nfc8e0f8ca04d47bb8e9e33493a7af30f rdf:first sg:person.0607221441.18
    227 rdf:rest N715cf95ad98949fa8e6b2101692a7a16
    228 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    229 schema:name Medical and Health Sciences
    230 rdf:type schema:DefinedTerm
    231 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    232 schema:name Immunology
    233 rdf:type schema:DefinedTerm
    234 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    235 schema:name Medical Microbiology
    236 rdf:type schema:DefinedTerm
    237 sg:grant.2410087 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1410
    238 rdf:type schema:MonetaryGrant
    239 sg:grant.2684826 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1410
    240 rdf:type schema:MonetaryGrant
    241 sg:grant.2695835 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1410
    242 rdf:type schema:MonetaryGrant
    243 sg:journal.1115214 schema:issn 1087-0156
    244 1546-1696
    245 schema:name Nature Biotechnology
    246 schema:publisher Springer Nature
    247 rdf:type schema:Periodical
    248 sg:person.01010776354.96 schema:affiliation grid-institutes:grid.421831.d
    249 schema:familyName Lee
    250 schema:givenName Gary
    251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010776354.96
    252 rdf:type schema:Person
    253 sg:person.01011534471.09 schema:affiliation grid-institutes:grid.421831.d
    254 schema:familyName Holmes
    255 schema:givenName Michael C
    256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011534471.09
    257 rdf:type schema:Person
    258 sg:person.01025207376.70 schema:affiliation grid-institutes:grid.421831.d
    259 schema:familyName Rebar
    260 schema:givenName Edward J
    261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025207376.70
    262 rdf:type schema:Person
    263 sg:person.010256663157.73 schema:affiliation grid-institutes:grid.25879.31
    264 schema:familyName Carroll
    265 schema:givenName Richard G
    266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010256663157.73
    267 rdf:type schema:Person
    268 sg:person.01057647671.77 schema:affiliation grid-institutes:grid.421831.d
    269 schema:familyName Guschin
    270 schema:givenName Dmitry Y
    271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01057647671.77
    272 rdf:type schema:Person
    273 sg:person.01065115346.16 schema:affiliation grid-institutes:grid.421831.d
    274 schema:familyName Urnov
    275 schema:givenName Fyodor D
    276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065115346.16
    277 rdf:type schema:Person
    278 sg:person.01106011641.25 schema:affiliation grid-institutes:grid.25879.31
    279 schema:familyName Liu
    280 schema:givenName Olga
    281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106011641.25
    282 rdf:type schema:Person
    283 sg:person.011354434034.56 schema:affiliation grid-institutes:grid.421831.d
    284 schema:familyName Wang
    285 schema:givenName Jianbin
    286 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011354434034.56
    287 rdf:type schema:Person
    288 sg:person.01174076271.35 schema:affiliation grid-institutes:grid.421831.d
    289 schema:familyName Miller
    290 schema:givenName Jeffrey C
    291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174076271.35
    292 rdf:type schema:Person
    293 sg:person.01203760001.73 schema:affiliation grid-institutes:grid.421831.d
    294 schema:familyName Kim
    295 schema:givenName Kenneth A
    296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203760001.73
    297 rdf:type schema:Person
    298 sg:person.01270353441.36 schema:affiliation grid-institutes:grid.421831.d
    299 schema:familyName Bartsevich
    300 schema:givenName Victor V
    301 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270353441.36
    302 rdf:type schema:Person
    303 sg:person.01336466641.02 schema:affiliation grid-institutes:grid.421831.d
    304 schema:familyName Lee
    305 schema:givenName Ya-Li
    306 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336466641.02
    307 rdf:type schema:Person
    308 sg:person.014235534277.63 schema:affiliation grid-institutes:grid.25879.31
    309 schema:familyName June
    310 schema:givenName Carl H
    311 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014235534277.63
    312 rdf:type schema:Person
    313 sg:person.015620210017.02 schema:affiliation grid-institutes:grid.421831.d
    314 schema:familyName Gregory
    315 schema:givenName Philip D
    316 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015620210017.02
    317 rdf:type schema:Person
    318 sg:person.0607221441.18 schema:affiliation grid-institutes:grid.239552.a
    319 schema:familyName Perez
    320 schema:givenName Elena E
    321 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607221441.18
    322 rdf:type schema:Person
    323 sg:person.0645571071.11 schema:affiliation grid-institutes:grid.421831.d
    324 schema:familyName Ando
    325 schema:givenName Dale
    326 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645571071.11
    327 rdf:type schema:Person
    328 sg:person.0661175741.04 schema:affiliation grid-institutes:grid.421831.d
    329 schema:familyName Rupniewski
    330 schema:givenName Igor
    331 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661175741.04
    332 rdf:type schema:Person
    333 sg:person.0725177232.35 schema:affiliation grid-institutes:grid.421831.d
    334 schema:familyName Waite
    335 schema:givenName Adam J
    336 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725177232.35
    337 rdf:type schema:Person
    338 sg:person.0732060266.84 schema:affiliation grid-institutes:grid.25879.31
    339 schema:familyName Carpenito
    340 schema:givenName Carmine
    341 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732060266.84
    342 rdf:type schema:Person
    343 sg:person.0753123553.37 schema:affiliation grid-institutes:grid.421831.d
    344 schema:familyName Wang
    345 schema:givenName Nathaniel
    346 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753123553.37
    347 rdf:type schema:Person
    348 sg:person.0770005773.92 schema:affiliation grid-institutes:grid.25879.31
    349 schema:familyName Riley
    350 schema:givenName James L
    351 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770005773.92
    352 rdf:type schema:Person
    353 sg:person.0771563241.19 schema:affiliation grid-institutes:None
    354 schema:familyName Jouvenot
    355 schema:givenName Yann
    356 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771563241.19
    357 rdf:type schema:Person
    358 sg:pub.10.1038/381661a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053562821
    359 https://doi.org/10.1038/381661a0
    360 rdf:type schema:CreativeWork
    361 sg:pub.10.1038/382722a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050488632
    362 https://doi.org/10.1038/382722a0
    363 rdf:type schema:CreativeWork
    364 sg:pub.10.1038/44385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016546162
    365 https://doi.org/10.1038/44385
    366 rdf:type schema:CreativeWork
    367 sg:pub.10.1038/90264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032644172
    368 https://doi.org/10.1038/90264
    369 rdf:type schema:CreativeWork
    370 sg:pub.10.1038/nature03556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040117781
    371 https://doi.org/10.1038/nature03556
    372 rdf:type schema:CreativeWork
    373 sg:pub.10.1038/nature03959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
    374 https://doi.org/10.1038/nature03959
    375 rdf:type schema:CreativeWork
    376 sg:pub.10.1038/nature04148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044521333
    377 https://doi.org/10.1038/nature04148
    378 rdf:type schema:CreativeWork
    379 sg:pub.10.1038/nbt1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002154526
    380 https://doi.org/10.1038/nbt1319
    381 rdf:type schema:CreativeWork
    382 sg:pub.10.1038/nbt1353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026122095
    383 https://doi.org/10.1038/nbt1353
    384 rdf:type schema:CreativeWork
    385 sg:pub.10.1038/nbt1367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008151467
    386 https://doi.org/10.1038/nbt1367
    387 rdf:type schema:CreativeWork
    388 sg:pub.10.1038/nm0102-47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018873794
    389 https://doi.org/10.1038/nm0102-47
    390 rdf:type schema:CreativeWork
    391 sg:pub.10.1038/nm1097-1052c schema:sameAs https://app.dimensions.ai/details/publication/pub.1051084444
    392 https://doi.org/10.1038/nm1097-1052c
    393 rdf:type schema:CreativeWork
    394 sg:pub.10.1038/nm1196-1240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026944069
    395 https://doi.org/10.1038/nm1196-1240
    396 rdf:type schema:CreativeWork
    397 sg:pub.10.1038/nm1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041122840
    398 https://doi.org/10.1038/nm1319
    399 rdf:type schema:CreativeWork
    400 sg:pub.10.1038/nm1326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024397965
    401 https://doi.org/10.1038/nm1326
    402 rdf:type schema:CreativeWork
    403 sg:pub.10.1038/nrd1087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040287926
    404 https://doi.org/10.1038/nrd1087
    405 rdf:type schema:CreativeWork
    406 sg:pub.10.1038/sj.onc.1206679 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024607188
    407 https://doi.org/10.1038/sj.onc.1206679
    408 rdf:type schema:CreativeWork
    409 grid-institutes:None schema:alternateName Present address: Process Science Department, Bayer Hematology/Cardiology, 800 Dwight Way, Berkeley, California 94701, USA.
    410 schema:name Present address: Process Science Department, Bayer Hematology/Cardiology, 800 Dwight Way, Berkeley, California 94701, USA.
    411 Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.
    412 rdf:type schema:Organization
    413 grid-institutes:grid.239552.a schema:alternateName Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA.
    414 schema:name Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.
    415 Division of Allergy and Immunology, Children's Hospital of Philadelphia, Joseph Stokes, Jr. Research Institute, 3615 Civic Center Blvd., Philadelphia, Pennsylvania 19104-4318, USA.
    416 rdf:type schema:Organization
    417 grid-institutes:grid.25879.31 schema:alternateName Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.
    418 schema:name Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, 421 Curie Blvd., Room 554, BRB II/III, Philadelphia, Pennsylvania 19104-6160, USA.
    419 rdf:type schema:Organization
    420 grid-institutes:grid.421831.d schema:alternateName Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.
    421 schema:name Sangamo BioSciences, Inc., Point Richmond Tech Center II, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.
    422 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...