Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-06

AUTHORS

Yannick Doyon, Jasmine M McCammon, Jeffrey C Miller, Farhoud Faraji, Catherine Ngo, George E Katibah, Rainier Amora, Toby D Hocking, Lei Zhang, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Sharon L Amacher

ABSTRACT

We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg. More... »

PAGES

702-708

Journal

TITLE

Nature Biotechnology

ISSUE

6

VOLUME

26

Related Patents

  • Glycan-Interacting Compounds
  • Glycan-Interacting Compounds
  • Use Of Cationic Lipids To Deliver Cas9
  • Methods For Correcting Presenilin Point Mutations
  • Engineered Crispr-Cas9 Nucleases
  • Using Rna-Guided Foki Nucleases (Rfns) To Increase Specificity For Rna-Guided Genome Editing
  • Determination Of In Vivo Dna Double-Strand Break Localization And Application Thereof
  • Genome Editing In Rats Using Zinc-Finger Nucleases
  • Methods For Nucleic Acid Editing
  • Homologous Recombination In The Oocyte
  • Determination Of In Vivo Dna Double-Strand Break Localization And Application Thereof
  • Evaluation And Improvement Of Nuclease Cleavage Specificity
  • Cas9 Proteins Including Ligand-Dependent Inteins
  • Method For The Generation Of Compact Tale-Nucleases And Uses Thereof
  • Optimal Soybean Loci
  • Genome Editing Using Targeting Endonucleases And Single-Stranded Nucleic Acids
  • Glycan-Interacting Compounds And Methods Of Use
  • Method For The Generation Of Compact Tale-Nucleases And Uses Thereof
  • Fad3 Performance Loci And Corresponding Target Site Specific Binding Proteins Capable Of Inducing Targeted Breaks
  • Gene Editing In The Oocyte By Cas9 Nucleases
  • Genome Editing In Rats Using Zinc-Finger Nucleases
  • Production Of Recombinant Proteins With Simple Glycoforms
  • Using Truncated Guide Rnas (Tru-Grnas) To Increase Specificity For Rna-Guided Genome Editing
  • Targeted Integration Into The Zp15 Locus
  • Organisms Homozygous For Targeted Modification
  • Production Of Fmdv-Resistant Livestock By Allele Substitution
  • Cells Deficient In Cmp-N-Acetylneuraminic Acid Hydroxylase And/Or Glycoprotein Alpha-1,3-Galactosyltransferase
  • Method For Increasing The Efficiency Of Double-Strand Break-Induced Mutagenssis
  • Organisms Homozygous For Targeted Modification
  • Production Of Recombinant Proteins With Simple Glycoforms
  • Method For Enhancing Rare-Cutting Endonuclease Efficiency And Uses Thereof
  • Inhibition Of Diacylglycerol Kinase To Augment Adoptive T Cell Transfer
  • Fusion Proteins Comprising A Dna-Binding Domain Of A Tal Effector Protein And A Non-Specific Cleavage Domain Of A Restriction Nuclease And Their Use
  • Method For Increasing The Efficiency Of Double-Strand Break-Induced Mutagenesis
  • Fad2 Performance Loci And Corresponding Target Site Specific Binding Proteins Capable Of Inducing Targeted Breaks
  • Using Rna-Guided Foki Nucleases (Rfns) To Increase Specificity For Rna-Guided Genome Editing
  • Method For Concentrating Cells That Are Genetically Altered By Nucleases
  • Increasing Specificity For Rna-Guided Genome Editing
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Fusion Proteins Comprising A Dna-Binding Domain Of A Tal Effector Protein And A Non-Specific Cleavage Domain Of A Restriction Nuclease And Their Use
  • Genome Editing In Rats Using Zinc-Finger Nucleases
  • Organisms Homozygous For Targeted Modification
  • Nuclease Profiling System
  • Ralen-Mediated Genetic Modification Techniques
  • Delivery Of Negatively Charged Proteins Using Cationic Lipids
  • Engineered Crispr-Cas9 Nucleases
  • Methods For Identifying A Target Site Of A Cas9 Nuclease
  • Methods And Compositions For Targeted Single-Stranded Cleavage And Targeted Integration
  • Delivery System For Functional Nucleases
  • Tal-Tet1 Fusion Proteins And Methods Of Use Thereof
  • Method For Concentrating Cells That Are Genetically Altered By Nucleases
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1409

    DOI

    http://dx.doi.org/10.1038/nbt1409

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045837461

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18500334


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Deoxyribonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Targeting", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutagenesis, Site-Directed", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Zebrafish", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Zebrafish Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Zinc Fingers", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Doyon", 
            "givenName": "Yannick", 
            "id": "sg:person.0650374030.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650374030.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California, Berkeley, California 94720-3200, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "McCammon", 
            "givenName": "Jasmine M", 
            "id": "sg:person.01171425561.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171425561.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Miller", 
            "givenName": "Jeffrey C", 
            "id": "sg:person.01174076271.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174076271.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Faraji", 
            "givenName": "Farhoud", 
            "id": "sg:person.01063417251.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063417251.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ngo", 
            "givenName": "Catherine", 
            "id": "sg:person.0636333263.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636333263.48"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Katibah", 
            "givenName": "George E", 
            "id": "sg:person.0752561663.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752561663.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amora", 
            "givenName": "Rainier", 
            "id": "sg:person.0663620734.69", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663620734.69"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hocking", 
            "givenName": "Toby D", 
            "id": "sg:person.01167575775.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167575775.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Lei", 
            "id": "sg:person.07755434327.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07755434327.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rebar", 
            "givenName": "Edward J", 
            "id": "sg:person.01025207376.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025207376.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Sangamo BioSciences (United States)", 
              "id": "https://www.grid.ac/institutes/grid.421831.d", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gregory", 
            "givenName": "Philip D", 
            "id": "sg:person.015620210017.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015620210017.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.", 
                "Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California, Berkeley, California 94720-3200, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Urnov", 
            "givenName": "Fyodor D", 
            "id": "sg:person.01065115346.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065115346.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California, Berkeley, California 94720-3200, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Amacher", 
            "givenName": "Sharon L", 
            "id": "sg:person.0767512025.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767512025.91"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1073/pnas.93.3.1156", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001210891"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0800940105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001216531"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1319", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002154526", 
              "https://doi.org/10.1038/nbt1319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m005341200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004387906"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/79951", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007182261", 
              "https://doi.org/10.1038/79951"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/79951", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007182261", 
              "https://doi.org/10.1038/79951"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg2091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007331949", 
              "https://doi.org/10.1038/nrg2091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg2091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007331949", 
              "https://doi.org/10.1038/nrg2091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bies.950170707", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008531007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/27.2.674", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011869221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(86)90463-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013895330"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(02)50961-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014345535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/aja.1002030302", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017782944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1116238", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023529415"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1206679", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024607188", 
              "https://doi.org/10.1038/sj.onc.1206679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1206679", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024607188", 
              "https://doi.org/10.1038/sj.onc.1206679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/22.25.5767", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025139704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0012-1606(89)80038-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027640353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/372642a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029991975", 
              "https://doi.org/10.1038/372642a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031654515", 
              "https://doi.org/10.1038/nrg1273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031654515", 
              "https://doi.org/10.1038/nrg1273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040117781", 
              "https://doi.org/10.1038/nature03556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040117781", 
              "https://doi.org/10.1038/nature03556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biochem.70.1.313", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041153456"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0605633103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041244111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0091-679x(08)61821-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041481271"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(05)80087-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041985668"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/29.12.e59", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047173511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ab.2004.09.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048737959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2028256", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062518816"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075091342", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03825.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1080096371"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082430239", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2008-06", 
        "datePublishedReg": "2008-06-01", 
        "description": "We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1409", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2516757", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2436517", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2520481", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "26"
          }
        ], 
        "name": "Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases", 
        "pagination": "702-708", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "da57939f008d906258838824badb077d0eac54f2b81675ce2c78321d77263800"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "18500334"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1409"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1045837461"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1409", 
          "https://app.dimensions.ai/details/publication/pub.1045837461"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T17:19", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt1409"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1409'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1409'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1409'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1409'


     

    This table displays all metadata directly associated to this object as RDF triples.

    292 TRIPLES      21 PREDICATES      67 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1409 schema:about N13da650ee7f240ca8bc8b9eb4079c2fa
    2 N21e6e2ae4b0a415394f922f387400647
    3 N443defe066564d8fad143da71795573b
    4 N4b9960988da24ebd98357d1e18bef2b7
    5 N4dac328cae794912bb86d9d5c83b2a94
    6 N8adc97da62194483acf4ac7ce571239a
    7 N8faf996f45a3410eab3dcaf53c378c15
    8 Na6684ab6da23473a94feb3fc35dbf08e
    9 Nc9e051ad767b46edb7930963289c9911
    10 Nf99e9a4f4ee34fee90cf2c5e212e6787
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author Nb600abd6617f4eb993afa2116521a180
    14 schema:citation sg:pub.10.1038/372642a0
    15 sg:pub.10.1038/79951
    16 sg:pub.10.1038/nature03556
    17 sg:pub.10.1038/nbt1319
    18 sg:pub.10.1038/nrg1273
    19 sg:pub.10.1038/nrg2091
    20 sg:pub.10.1038/sj.onc.1206679
    21 https://app.dimensions.ai/details/publication/pub.1075091342
    22 https://app.dimensions.ai/details/publication/pub.1082430239
    23 https://doi.org/10.1002/aja.1002030302
    24 https://doi.org/10.1002/bies.950170707
    25 https://doi.org/10.1002/j.1460-2075.1985.tb03825.x
    26 https://doi.org/10.1016/0092-8674(86)90463-0
    27 https://doi.org/10.1016/j.ab.2004.09.004
    28 https://doi.org/10.1016/s0012-1606(89)80038-7
    29 https://doi.org/10.1016/s0076-6879(02)50961-7
    30 https://doi.org/10.1016/s0091-679x(08)61821-x
    31 https://doi.org/10.1016/s0092-8674(05)80087-x
    32 https://doi.org/10.1073/pnas.0605633103
    33 https://doi.org/10.1073/pnas.0800940105
    34 https://doi.org/10.1073/pnas.93.3.1156
    35 https://doi.org/10.1074/jbc.m005341200
    36 https://doi.org/10.1093/nar/22.25.5767
    37 https://doi.org/10.1093/nar/27.2.674
    38 https://doi.org/10.1093/nar/29.12.e59
    39 https://doi.org/10.1126/science.1116238
    40 https://doi.org/10.1126/science.2028256
    41 https://doi.org/10.1146/annurev.biochem.70.1.313
    42 schema:datePublished 2008-06
    43 schema:datePublishedReg 2008-06-01
    44 schema:description We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
    45 schema:genre research_article
    46 schema:inLanguage en
    47 schema:isAccessibleForFree true
    48 schema:isPartOf N1c6d056184db44b08a6cd60c3ea7bbee
    49 Nc74598f5359a4c85b64638ad6d929c0c
    50 sg:journal.1115214
    51 schema:name Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
    52 schema:pagination 702-708
    53 schema:productId N1db13969ffd44b2aa7cb269d73361ee4
    54 N21af6771fea74311a4e5b7c7abe4a18e
    55 N2ba43e93a438452d9deaeac5f0384cdb
    56 N4bfb95e8c74648b6832da66af262eb2b
    57 Nad006702f7a141d19b3ee5a6c1eb64e9
    58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045837461
    59 https://doi.org/10.1038/nbt1409
    60 schema:sdDatePublished 2019-04-10T17:19
    61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    62 schema:sdPublisher N8ac67c0e3f484a7a8d35a49e4a92bad1
    63 schema:url http://www.nature.com/articles/nbt1409
    64 sgo:license sg:explorer/license/
    65 sgo:sdDataset articles
    66 rdf:type schema:ScholarlyArticle
    67 N104e30cf27644658a9fbb6c23d270fba rdf:first sg:person.0663620734.69
    68 rdf:rest N5f1f48ee600446d18e86a2a98e1b667b
    69 N13da650ee7f240ca8bc8b9eb4079c2fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name Animals
    71 rdf:type schema:DefinedTerm
    72 N1c6d056184db44b08a6cd60c3ea7bbee schema:issueNumber 6
    73 rdf:type schema:PublicationIssue
    74 N1db13969ffd44b2aa7cb269d73361ee4 schema:name nlm_unique_id
    75 schema:value 9604648
    76 rdf:type schema:PropertyValue
    77 N1e77eeab944b4fe880e42f51f2d4567f rdf:first sg:person.07755434327.03
    78 rdf:rest Na1a435f433214bf2a9f99fb7c838cbe9
    79 N21af6771fea74311a4e5b7c7abe4a18e schema:name readcube_id
    80 schema:value da57939f008d906258838824badb077d0eac54f2b81675ce2c78321d77263800
    81 rdf:type schema:PropertyValue
    82 N21e6e2ae4b0a415394f922f387400647 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    83 schema:name Animals, Genetically Modified
    84 rdf:type schema:DefinedTerm
    85 N2ba43e93a438452d9deaeac5f0384cdb schema:name doi
    86 schema:value 10.1038/nbt1409
    87 rdf:type schema:PropertyValue
    88 N2fa6cbe8ae6f4e05b3aa3a5b6163f700 rdf:first sg:person.01065115346.16
    89 rdf:rest N66da24d7651f451c98094d364c73f5c5
    90 N42f4898d51ce4476892f116a2d44d844 rdf:first sg:person.01171425561.14
    91 rdf:rest N785c79f058e942a489181ec6adbc9146
    92 N443defe066564d8fad143da71795573b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Protein Engineering
    94 rdf:type schema:DefinedTerm
    95 N4b9960988da24ebd98357d1e18bef2b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Deoxyribonucleases
    97 rdf:type schema:DefinedTerm
    98 N4bfb95e8c74648b6832da66af262eb2b schema:name pubmed_id
    99 schema:value 18500334
    100 rdf:type schema:PropertyValue
    101 N4cd396e92e224084acea1101f124e895 rdf:first sg:person.015620210017.02
    102 rdf:rest N2fa6cbe8ae6f4e05b3aa3a5b6163f700
    103 N4dac328cae794912bb86d9d5c83b2a94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Zebrafish Proteins
    105 rdf:type schema:DefinedTerm
    106 N5f1f48ee600446d18e86a2a98e1b667b rdf:first sg:person.01167575775.01
    107 rdf:rest N1e77eeab944b4fe880e42f51f2d4567f
    108 N66da24d7651f451c98094d364c73f5c5 rdf:first sg:person.0767512025.91
    109 rdf:rest rdf:nil
    110 N775abd81297f4aafb6b494c4b61d9778 rdf:first sg:person.01063417251.26
    111 rdf:rest N82ce4c579c3e4509a5ef73aa09bf01af
    112 N785c79f058e942a489181ec6adbc9146 rdf:first sg:person.01174076271.35
    113 rdf:rest N775abd81297f4aafb6b494c4b61d9778
    114 N82ce4c579c3e4509a5ef73aa09bf01af rdf:first sg:person.0636333263.48
    115 rdf:rest Na21a981aaee14e2ea175ed09f59d9a06
    116 N8ac67c0e3f484a7a8d35a49e4a92bad1 schema:name Springer Nature - SN SciGraph project
    117 rdf:type schema:Organization
    118 N8adc97da62194483acf4ac7ce571239a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Zinc Fingers
    120 rdf:type schema:DefinedTerm
    121 N8faf996f45a3410eab3dcaf53c378c15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Genetic Engineering
    123 rdf:type schema:DefinedTerm
    124 Na1a435f433214bf2a9f99fb7c838cbe9 rdf:first sg:person.01025207376.70
    125 rdf:rest N4cd396e92e224084acea1101f124e895
    126 Na21a981aaee14e2ea175ed09f59d9a06 rdf:first sg:person.0752561663.94
    127 rdf:rest N104e30cf27644658a9fbb6c23d270fba
    128 Na6684ab6da23473a94feb3fc35dbf08e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Mutagenesis, Site-Directed
    130 rdf:type schema:DefinedTerm
    131 Nad006702f7a141d19b3ee5a6c1eb64e9 schema:name dimensions_id
    132 schema:value pub.1045837461
    133 rdf:type schema:PropertyValue
    134 Nb600abd6617f4eb993afa2116521a180 rdf:first sg:person.0650374030.02
    135 rdf:rest N42f4898d51ce4476892f116a2d44d844
    136 Nc74598f5359a4c85b64638ad6d929c0c schema:volumeNumber 26
    137 rdf:type schema:PublicationVolume
    138 Nc9e051ad767b46edb7930963289c9911 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Zebrafish
    140 rdf:type schema:DefinedTerm
    141 Nf99e9a4f4ee34fee90cf2c5e212e6787 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Gene Targeting
    143 rdf:type schema:DefinedTerm
    144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Biological Sciences
    146 rdf:type schema:DefinedTerm
    147 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    148 schema:name Genetics
    149 rdf:type schema:DefinedTerm
    150 sg:grant.2436517 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1409
    151 rdf:type schema:MonetaryGrant
    152 sg:grant.2516757 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1409
    153 rdf:type schema:MonetaryGrant
    154 sg:grant.2520481 http://pending.schema.org/fundedItem sg:pub.10.1038/nbt1409
    155 rdf:type schema:MonetaryGrant
    156 sg:journal.1115214 schema:issn 1087-0156
    157 1546-1696
    158 schema:name Nature Biotechnology
    159 rdf:type schema:Periodical
    160 sg:person.01025207376.70 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    161 schema:familyName Rebar
    162 schema:givenName Edward J
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025207376.70
    164 rdf:type schema:Person
    165 sg:person.01063417251.26 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    166 schema:familyName Faraji
    167 schema:givenName Farhoud
    168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063417251.26
    169 rdf:type schema:Person
    170 sg:person.01065115346.16 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    171 schema:familyName Urnov
    172 schema:givenName Fyodor D
    173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065115346.16
    174 rdf:type schema:Person
    175 sg:person.01167575775.01 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    176 schema:familyName Hocking
    177 schema:givenName Toby D
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167575775.01
    179 rdf:type schema:Person
    180 sg:person.01171425561.14 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    181 schema:familyName McCammon
    182 schema:givenName Jasmine M
    183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171425561.14
    184 rdf:type schema:Person
    185 sg:person.01174076271.35 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    186 schema:familyName Miller
    187 schema:givenName Jeffrey C
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174076271.35
    189 rdf:type schema:Person
    190 sg:person.015620210017.02 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    191 schema:familyName Gregory
    192 schema:givenName Philip D
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015620210017.02
    194 rdf:type schema:Person
    195 sg:person.0636333263.48 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    196 schema:familyName Ngo
    197 schema:givenName Catherine
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636333263.48
    199 rdf:type schema:Person
    200 sg:person.0650374030.02 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    201 schema:familyName Doyon
    202 schema:givenName Yannick
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650374030.02
    204 rdf:type schema:Person
    205 sg:person.0663620734.69 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    206 schema:familyName Amora
    207 schema:givenName Rainier
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663620734.69
    209 rdf:type schema:Person
    210 sg:person.0752561663.94 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    211 schema:familyName Katibah
    212 schema:givenName George E
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752561663.94
    214 rdf:type schema:Person
    215 sg:person.0767512025.91 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    216 schema:familyName Amacher
    217 schema:givenName Sharon L
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767512025.91
    219 rdf:type schema:Person
    220 sg:person.07755434327.03 schema:affiliation https://www.grid.ac/institutes/grid.421831.d
    221 schema:familyName Zhang
    222 schema:givenName Lei
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07755434327.03
    224 rdf:type schema:Person
    225 sg:pub.10.1038/372642a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029991975
    226 https://doi.org/10.1038/372642a0
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1038/79951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007182261
    229 https://doi.org/10.1038/79951
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1038/nature03556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040117781
    232 https://doi.org/10.1038/nature03556
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1038/nbt1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002154526
    235 https://doi.org/10.1038/nbt1319
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1038/nrg1273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031654515
    238 https://doi.org/10.1038/nrg1273
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1038/nrg2091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007331949
    241 https://doi.org/10.1038/nrg2091
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/sj.onc.1206679 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024607188
    244 https://doi.org/10.1038/sj.onc.1206679
    245 rdf:type schema:CreativeWork
    246 https://app.dimensions.ai/details/publication/pub.1075091342 schema:CreativeWork
    247 https://app.dimensions.ai/details/publication/pub.1082430239 schema:CreativeWork
    248 https://doi.org/10.1002/aja.1002030302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017782944
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1002/bies.950170707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008531007
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1002/j.1460-2075.1985.tb03825.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1080096371
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/0092-8674(86)90463-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013895330
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1016/j.ab.2004.09.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048737959
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1016/s0012-1606(89)80038-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027640353
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1016/s0076-6879(02)50961-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014345535
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1016/s0091-679x(08)61821-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041481271
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1016/s0092-8674(05)80087-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041985668
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1073/pnas.0605633103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041244111
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1073/pnas.0800940105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001216531
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1073/pnas.93.3.1156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001210891
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1074/jbc.m005341200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004387906
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1093/nar/22.25.5767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025139704
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1093/nar/27.2.674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011869221
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1093/nar/29.12.e59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047173511
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1126/science.1116238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023529415
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1126/science.2028256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062518816
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1146/annurev.biochem.70.1.313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041153456
    285 rdf:type schema:CreativeWork
    286 https://www.grid.ac/institutes/grid.421831.d schema:alternateName Sangamo BioSciences (United States)
    287 schema:name Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.
    288 rdf:type schema:Organization
    289 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
    290 schema:name Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California, Berkeley, California 94720-3200, USA.
    291 Sangamo BioSciences, Inc., Pt. Richmond Tech Center, 501 Canal Blvd., Suite A100, Richmond, California 94804, USA.
    292 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...