Ontology type: schema:ScholarlyArticle Open Access: True
2008-03
AUTHORSGary K Geiss, Roger E Bumgarner, Brian Birditt, Timothy Dahl, Naeem Dowidar, Dwayne L Dunaway, H Perry Fell, Sean Ferree, Renee D George, Tammy Grogan, Jeffrey J James, Malini Maysuria, Jeffrey D Mitton, Paola Oliveri, Jennifer L Osborn, Tao Peng, Amber L Ratcliffe, Philippa J Webster, Eric H Davidson, Leroy Hood, Krassen Dimitrov
ABSTRACTWe describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels. More... »
PAGES317-325
http://scigraph.springernature.com/pub.10.1038/nbt1385
DOIhttp://dx.doi.org/10.1038/nbt1385
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1053259870
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/18278033
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Color",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Probes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Profiling",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Library",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genes, Reporter",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Image Processing, Computer-Assisted",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nanotechnology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oligonucleotide Array Sequence Analysis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Poliovirus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polymerase Chain Reaction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Messenger",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Reproducibility of Results",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sensitivity and Specificity",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Geiss",
"givenName": "Gary K",
"id": "sg:person.01352127342.77",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352127342.77"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"The Department of Microbiology, Box 358070, University of Washington, Seattle, Washington 98195, USA."
],
"type": "Organization"
},
"familyName": "Bumgarner",
"givenName": "Roger E",
"id": "sg:person.0753657626.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753657626.45"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Birditt",
"givenName": "Brian",
"id": "sg:person.01040233740.21",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040233740.21"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Dahl",
"givenName": "Timothy",
"id": "sg:person.0762255163.31",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762255163.31"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Dowidar",
"givenName": "Naeem",
"id": "sg:person.01030370363.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030370363.05"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Dunaway",
"givenName": "Dwayne L",
"id": "sg:person.01076503563.02",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076503563.02"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Fell",
"givenName": "H Perry",
"id": "sg:person.01144616763.55",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144616763.55"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Ferree",
"givenName": "Sean",
"id": "sg:person.01212732163.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212732163.45"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "George",
"givenName": "Renee D",
"id": "sg:person.01261045363.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261045363.68"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Grogan",
"givenName": "Tammy",
"id": "sg:person.01327160563.11",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327160563.11"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "James",
"givenName": "Jeffrey J",
"id": "sg:person.0603554463.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603554463.14"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Maysuria",
"givenName": "Malini",
"id": "sg:person.0651667663.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651667663.23"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Mitton",
"givenName": "Jeffrey D",
"id": "sg:person.0720003063.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720003063.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "California Institute of Technology",
"id": "https://www.grid.ac/institutes/grid.20861.3d",
"name": [
"Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA."
],
"type": "Organization"
},
"familyName": "Oliveri",
"givenName": "Paola",
"id": "sg:person.01030452602.80",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030452602.80"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Osborn",
"givenName": "Jennifer L",
"id": "sg:person.01034231463.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034231463.68"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"The Department of Microbiology, Box 358070, University of Washington, Seattle, Washington 98195, USA."
],
"type": "Organization"
},
"familyName": "Peng",
"givenName": "Tao",
"id": "sg:person.0770103136.92",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770103136.92"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Ratcliffe",
"givenName": "Amber L",
"id": "sg:person.01150460063.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150460063.51"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA."
],
"type": "Organization"
},
"familyName": "Webster",
"givenName": "Philippa J",
"id": "sg:person.013504556353.43",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013504556353.43"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "California Institute of Technology",
"id": "https://www.grid.ac/institutes/grid.20861.3d",
"name": [
"Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA."
],
"type": "Organization"
},
"familyName": "Davidson",
"givenName": "Eric H",
"id": "sg:person.07732026722.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07732026722.27"
],
"type": "Person"
},
{
"familyName": "Hood",
"givenName": "Leroy",
"id": "sg:person.016174641237.97",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016174641237.97"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute for Systems Biology",
"id": "https://www.grid.ac/institutes/grid.64212.33",
"name": [
"The Institute of Systems Biology, 1441 N. 34th St., Seattle, Washington 98103, USA."
],
"type": "Organization"
},
"familyName": "Dimitrov",
"givenName": "Krassen",
"id": "sg:person.01323167246.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323167246.04"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1006/dbio.2000.9941",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002755525"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1242",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007714455",
"https://doi.org/10.1038/nbt1242"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1242",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007714455",
"https://doi.org/10.1038/nbt1242"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-2836(05)80360-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013618994"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/dbio.2002.0627",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013845403"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkg091",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015137191"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/gb-2004-5-10-r80",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018457673",
"https://doi.org/10.1186/gb-2004-5-10-r80"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/28.22.4552",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023751575"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(76)90139-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032741297"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1236",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037539567",
"https://doi.org/10.1038/nbt1236"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1236",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037539567",
"https://doi.org/10.1038/nbt1236"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1239",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037875102",
"https://doi.org/10.1038/nbt1239"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1239",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037875102",
"https://doi.org/10.1038/nbt1239"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gki025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038073766"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gki914",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038122314"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1056/nejmoa021967",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038600096"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/70487",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040306630",
"https://doi.org/10.1038/70487"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/70487",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040306630",
"https://doi.org/10.1038/70487"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.286.5439.531",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042995627"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/415530a",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043001094",
"https://doi.org/10.1038/415530a"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/415530a",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043001094",
"https://doi.org/10.1038/415530a"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1217",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044623466",
"https://doi.org/10.1038/nbt1217"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1217",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044623466",
"https://doi.org/10.1038/nbt1217"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1200/jco.2005.02.8712",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1064204200"
],
"type": "CreativeWork"
}
],
"datePublished": "2008-03",
"datePublishedReg": "2008-03-01",
"description": "We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels.",
"genre": "research_article",
"id": "sg:pub.10.1038/nbt1385",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1115214",
"issn": [
"1087-0156",
"1546-1696"
],
"name": "Nature Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "26"
}
],
"name": "Direct multiplexed measurement of gene expression with color-coded probe pairs",
"pagination": "317-325",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"fdb012d8444f175b83aee30233726a36d32af8c16a4024e7e3da2f63248ed648"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"18278033"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9604648"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nbt1385"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1053259870"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nbt1385",
"https://app.dimensions.ai/details/publication/pub.1053259870"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T12:59",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000426.jsonl",
"type": "ScholarlyArticle",
"url": "http://www.nature.com/articles/nbt1385"
}
]Download the RDF metadata as:Â json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1385'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1385'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1385'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1385'
This table displays all metadata directly associated to this object as RDF triples.
366 TRIPLES
21 PREDICATES
62 URIs
36 LITERALS
24 BLANK NODES