Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11-30

AUTHORS

Masato Nakagawa, Michiyo Koyanagi, Koji Tanabe, Kazutoshi Takahashi, Tomoko Ichisaka, Takashi Aoi, Keisuke Okita, Yuji Mochiduki, Nanako Takizawa, Shinya Yamanaka

ABSTRACT

Direct reprogramming of somatic cells provides an opportunity to generate patient- or disease-specific pluripotent stem cells. Such induced pluripotent stem (iPS) cells were generated from mouse fibroblasts by retroviral transduction of four transcription factors: Oct3/4, Sox2, Klf4 and c-Myc1. Mouse iPS cells are indistinguishable from embryonic stem (ES) cells in many respects and produce germline-competent chimeras2,3,4. Reactivation of the c-Myc retrovirus, however, increases tumorigenicity in the chimeras and progeny mice, hindering clinical applications3. Here we describe a modified protocol for the generation of iPS cells that does not require the Myc retrovirus. With this protocol, we obtained significantly fewer non-iPS background cells, and the iPS cells generated were consistently of high quality. Mice derived from Myc− iPS cells did not develop tumors during the study period. The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC. More... »

PAGES

101-106

Journal

TITLE

Nature Biotechnology

ISSUE

1

VOLUME

26

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1374

    DOI

    http://dx.doi.org/10.1038/nbt1374

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002744128

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18059259


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