Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-10

AUTHORS

Alexander Meissner, Marius Wernig, Rudolf Jaenisch

ABSTRACT

In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of a neomycin-resistance gene inserted into the endogenous Oct4 (also known as Pou5f1) or Nanog loci. Direct isolation of pluripotent cells from cultured somatic cells is of potential therapeutic interest, but translation to human systems would be hindered by the requirement for transgenic donors in the present iPS isolation protocol. Here we demonstrate that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria. More... »

PAGES

1177-1181

Journal

TITLE

Nature Biotechnology

ISSUE

10

VOLUME

25

Related Patents

  • Induction Of Pluripotent Cells
  • Method For Reprogramming Differentiated Cells
  • New Serum-Free Medium For Inducing Pluripotent Stem Cells Quickly With High Efficiency And Method Using Thereof
  • Induced Pluripotent Stem Cells Produced With Oct3/4, Klf4 And Sox2
  • Generation Of Pluripotent Cells From Fibroblasts
  • Reprogramming Of Somatic Cells
  • Nuclear Reprogramming Factor Comprising Mirna And A Protein Factor
  • Culture Media, Cell Cultures And Methods Of Culturing Pluripotent Stem Cells In An Undifferentiated State
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • De-Differentiation Of Human Cells
  • Reprogramming Of Somatic Cells
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Reprogramming Cells
  • Method For Producing Neural Stem Cells
  • Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Efficient Method For Nuclear Reprogramming
  • Generation Of Patient-Specific Differentiated Cell Types By Epigenetic Induction
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Methods And Platforms For Drug Discovery Using Induced Pluripotent Stem Cells
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Epigenetic Induction Of Human Patient-Specific Self-Propagating Dopaminergic Neuron Cell
  • Generation Of Patient-Specific Differentiated Cell Types By Epigenetic Induction
  • Methods And Materials For Obtaining Induced Pluripotent Stem Cells
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Generation Of Induced Pluripotent Stem (Ips) Cells
  • Wnt Pathway Stimulation In Reprogramming Somatic Cells
  • Generation Of Pluripotent Cells From Fibroblasts
  • Culture Method Of Embryoid Bodies And/Or Neural Stem Cells Derived From Human Differentiated Cell-Derived Pluripotent Stem Cells
  • Method For Preparing Induced Pluripotent Stem Cells And Culture Medium Used For Preparing Induced Pluripotent Stem Cells
  • Wnt Pathway Stimulation In Reprogramming Somatic Cells
  • Reprogramming Cells
  • Induction Of Pluripotent Cells
  • Providing Ipscs To A Customer
  • Methods For The Production Of Ips Cells Using Non-Viral Approach
  • Nuclear Reprogramming Factor
  • De-Differentiation Of Human Cells
  • Generation Of Induced Pluripotent Stem (Ips) Cells
  • Methods For The Production Of Ips Cells
  • Programming And Reprogramming Of Cells
  • Reprogramming Somatic Cells Using Retroviral Vectors Comprising Oct-4 And Sox2 Genes
  • Wnt Pathway Stimulation In Reprogramming Somatic Cells With Nuclear Reprogramming Factors
  • Wnt Pathway Stimulation In Reprogramming Somatic Cells
  • Oct3/4, Klf4, C-Myc And Sox2 Produce Induced Pluripotent Stem Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Method For Producing Neurospheres
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Wnt Pathway Stimulation In Reprogramming Somatic Cells
  • Compositions And Methods For Mammalian Genetics And Uses Thereof
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Reprogramming T Cells And Hematopoietic Cells
  • Method Of Effecting De-Differentiation Of A Cell
  • Production Of Pluripotent Cells Through Inhibition Of Bright/Arid3a Function
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1335

    DOI

    http://dx.doi.org/10.1038/nbt1335

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049953935

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17724450


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotechnology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Lineage", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA-Binding Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Female", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Kruppel-Like Transcription Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Octamer Transcription Factor-3", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Proto-Oncogene Proteins c-myc", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "SOXB1 Transcription Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Trans-Activators", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transduction, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Massachusetts Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meissner", 
            "givenName": "Alexander", 
            "id": "sg:person.016035276077.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016035276077.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Massachusetts Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wernig", 
            "givenName": "Marius", 
            "id": "sg:person.01256420377.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256420377.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Massachusetts Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA.", 
                "Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jaenisch", 
            "givenName": "Rudolf", 
            "id": "sg:person.015376426517.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015376426517.48"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05879", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003888215", 
              "https://doi.org/10.1038/nature05879"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009469255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014573758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04955", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014595021", 
              "https://doi.org/10.1038/nature04955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04955", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014595021", 
              "https://doi.org/10.1038/nature04955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04955", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014595021", 
              "https://doi.org/10.1038/nature04955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molbrainres.2004.09.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016575240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.101118898", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016959164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019109862", 
              "https://doi.org/10.1038/nature05944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ydbio.2004.06.026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021141005"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.blre.2005.01.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022275744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1523/jneurosci.0428-04.200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024945874"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1116447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032194044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0960-9822(01)00459-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037472156"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1973", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039230968", 
              "https://doi.org/10.1038/ng1973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1750-3639.2006.00010.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046892126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078227860", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082943978", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2007-10", 
        "datePublishedReg": "2007-10-01", 
        "description": "In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of a neomycin-resistance gene inserted into the endogenous Oct4 (also known as Pou5f1) or Nanog loci. Direct isolation of pluripotent cells from cultured somatic cells is of potential therapeutic interest, but translation to human systems would be hindered by the requirement for transgenic donors in the present iPS isolation protocol. Here we demonstrate that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1335", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "25"
          }
        ], 
        "name": "Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells", 
        "pagination": "1177-1181", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "edf6db8a9227a764e53438a46b3f97afba683184b05a2586e522de74bbb60398"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "17724450"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1335"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1049953935"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1335", 
          "https://app.dimensions.ai/details/publication/pub.1049953935"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:29", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt1335"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1335'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1335'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1335'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1335'


     

    This table displays all metadata directly associated to this object as RDF triples.

    209 TRIPLES      21 PREDICATES      64 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1335 schema:about N04a5ca4032ec4df38da02eab834b27ca
    2 N05d46823bd4b41259f4dd1ecb4b42a33
    3 N2002241dd4aa44e180d8091f2b21bf11
    4 N20c4b761f711413ebd4a6043b2499607
    5 N2cd1fb381ba4429f922a77200914a275
    6 N2f92e6e1811942659af50863dfecf1e5
    7 N38bc3fb1fc67420e8919d2933acc79b7
    8 N5ac660e446b34bfcb8bbe8b26d3a818d
    9 N5d4618e7885142d4ad61fb5626f6e2ec
    10 N86d4091c769c4522a3c9fed00898982c
    11 N9a7f7bd0d28540169de42c5db5111b45
    12 Ncdd75cb74a0947308970ac0ba8385992
    13 Nddafee9cb84848eb877ddad84ad54d96
    14 Ne5cb130b4fb248b1a6c027eee3ee11f4
    15 Necb5ccf65f454a7db6bbe3437d122225
    16 Nf5e97df7e9164d96b74add2e55f11c23
    17 Nff7221c251354c06a12e45ba068d46fe
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author N74ae5ed064984b16b8b5c9bb83ccbeef
    21 schema:citation sg:pub.10.1038/nature04955
    22 sg:pub.10.1038/nature05879
    23 sg:pub.10.1038/nature05934
    24 sg:pub.10.1038/nature05944
    25 sg:pub.10.1038/ng1973
    26 https://app.dimensions.ai/details/publication/pub.1078227860
    27 https://app.dimensions.ai/details/publication/pub.1082943978
    28 https://doi.org/10.1016/j.blre.2005.01.005
    29 https://doi.org/10.1016/j.cell.2006.07.024
    30 https://doi.org/10.1016/j.molbrainres.2004.09.002
    31 https://doi.org/10.1016/j.stem.2007.05.012
    32 https://doi.org/10.1016/j.stem.2007.05.014
    33 https://doi.org/10.1016/j.ydbio.2004.06.026
    34 https://doi.org/10.1016/s0960-9822(01)00459-6
    35 https://doi.org/10.1073/pnas.101118898
    36 https://doi.org/10.1111/j.1750-3639.2006.00010.x
    37 https://doi.org/10.1126/science.1116447
    38 https://doi.org/10.1523/jneurosci.0428-04.200
    39 schema:datePublished 2007-10
    40 schema:datePublishedReg 2007-10-01
    41 schema:description In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell-like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4. In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of a neomycin-resistance gene inserted into the endogenous Oct4 (also known as Pou5f1) or Nanog loci. Direct isolation of pluripotent cells from cultured somatic cells is of potential therapeutic interest, but translation to human systems would be hindered by the requirement for transgenic donors in the present iPS isolation protocol. Here we demonstrate that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.
    42 schema:genre research_article
    43 schema:inLanguage en
    44 schema:isAccessibleForFree false
    45 schema:isPartOf N154bb2ff46c144c8bad8c78051958b38
    46 Nf42346c09e344d779c22c2af771fd672
    47 sg:journal.1115214
    48 schema:name Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells
    49 schema:pagination 1177-1181
    50 schema:productId N366a98ab37f74db386fd80f15c1faf1a
    51 N8cc8fe8897824d86b6ee1d7009bd4e3b
    52 Nada8cbbe3f6e47c89f894c47e6a75c3c
    53 Nd5919ee2ed7b41bba5f41ae95c34a742
    54 Nd5b367c8f0b44491bbbdacf8b3172ec6
    55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049953935
    56 https://doi.org/10.1038/nbt1335
    57 schema:sdDatePublished 2019-04-10T16:29
    58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    59 schema:sdPublisher N310bca78977a4f0982c6c9b2a08a143a
    60 schema:url http://www.nature.com/articles/nbt1335
    61 sgo:license sg:explorer/license/
    62 sgo:sdDataset articles
    63 rdf:type schema:ScholarlyArticle
    64 N04a5ca4032ec4df38da02eab834b27ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    65 schema:name Cells, Cultured
    66 rdf:type schema:DefinedTerm
    67 N05d46823bd4b41259f4dd1ecb4b42a33 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    68 schema:name Trans-Activators
    69 rdf:type schema:DefinedTerm
    70 N154bb2ff46c144c8bad8c78051958b38 schema:issueNumber 10
    71 rdf:type schema:PublicationIssue
    72 N2002241dd4aa44e180d8091f2b21bf11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Cell Differentiation
    74 rdf:type schema:DefinedTerm
    75 N20c4b761f711413ebd4a6043b2499607 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name DNA-Binding Proteins
    77 rdf:type schema:DefinedTerm
    78 N2cd1fb381ba4429f922a77200914a275 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Mice
    80 rdf:type schema:DefinedTerm
    81 N2f92e6e1811942659af50863dfecf1e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Fibroblasts
    83 rdf:type schema:DefinedTerm
    84 N310bca78977a4f0982c6c9b2a08a143a schema:name Springer Nature - SN SciGraph project
    85 rdf:type schema:Organization
    86 N366a98ab37f74db386fd80f15c1faf1a schema:name pubmed_id
    87 schema:value 17724450
    88 rdf:type schema:PropertyValue
    89 N38bc3fb1fc67420e8919d2933acc79b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Octamer Transcription Factor-3
    91 rdf:type schema:DefinedTerm
    92 N5ac660e446b34bfcb8bbe8b26d3a818d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Biotechnology
    94 rdf:type schema:DefinedTerm
    95 N5d4618e7885142d4ad61fb5626f6e2ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Cell Lineage
    97 rdf:type schema:DefinedTerm
    98 N74ae5ed064984b16b8b5c9bb83ccbeef rdf:first sg:person.016035276077.37
    99 rdf:rest N9f5062ad4815452db827929cfec037c3
    100 N86d4091c769c4522a3c9fed00898982c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Kruppel-Like Transcription Factors
    102 rdf:type schema:DefinedTerm
    103 N8cc8fe8897824d86b6ee1d7009bd4e3b schema:name doi
    104 schema:value 10.1038/nbt1335
    105 rdf:type schema:PropertyValue
    106 N9a7f7bd0d28540169de42c5db5111b45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Male
    108 rdf:type schema:DefinedTerm
    109 N9f5062ad4815452db827929cfec037c3 rdf:first sg:person.01256420377.73
    110 rdf:rest Nc1e3b7b6ba964d828f52d7a80c9e38f7
    111 Nada8cbbe3f6e47c89f894c47e6a75c3c schema:name readcube_id
    112 schema:value edf6db8a9227a764e53438a46b3f97afba683184b05a2586e522de74bbb60398
    113 rdf:type schema:PropertyValue
    114 Nc1e3b7b6ba964d828f52d7a80c9e38f7 rdf:first sg:person.015376426517.48
    115 rdf:rest rdf:nil
    116 Ncdd75cb74a0947308970ac0ba8385992 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name SOXB1 Transcription Factors
    118 rdf:type schema:DefinedTerm
    119 Nd5919ee2ed7b41bba5f41ae95c34a742 schema:name nlm_unique_id
    120 schema:value 9604648
    121 rdf:type schema:PropertyValue
    122 Nd5b367c8f0b44491bbbdacf8b3172ec6 schema:name dimensions_id
    123 schema:value pub.1049953935
    124 rdf:type schema:PropertyValue
    125 Nddafee9cb84848eb877ddad84ad54d96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Female
    127 rdf:type schema:DefinedTerm
    128 Ne5cb130b4fb248b1a6c027eee3ee11f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Proto-Oncogene Proteins c-myc
    130 rdf:type schema:DefinedTerm
    131 Necb5ccf65f454a7db6bbe3437d122225 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Animals
    133 rdf:type schema:DefinedTerm
    134 Nf42346c09e344d779c22c2af771fd672 schema:volumeNumber 25
    135 rdf:type schema:PublicationVolume
    136 Nf5e97df7e9164d96b74add2e55f11c23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Transduction, Genetic
    138 rdf:type schema:DefinedTerm
    139 Nff7221c251354c06a12e45ba068d46fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Pluripotent Stem Cells
    141 rdf:type schema:DefinedTerm
    142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    143 schema:name Biological Sciences
    144 rdf:type schema:DefinedTerm
    145 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    146 schema:name Biochemistry and Cell Biology
    147 rdf:type schema:DefinedTerm
    148 sg:journal.1115214 schema:issn 1087-0156
    149 1546-1696
    150 schema:name Nature Biotechnology
    151 rdf:type schema:Periodical
    152 sg:person.01256420377.73 schema:affiliation https://www.grid.ac/institutes/grid.116068.8
    153 schema:familyName Wernig
    154 schema:givenName Marius
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256420377.73
    156 rdf:type schema:Person
    157 sg:person.015376426517.48 schema:affiliation https://www.grid.ac/institutes/grid.116068.8
    158 schema:familyName Jaenisch
    159 schema:givenName Rudolf
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015376426517.48
    161 rdf:type schema:Person
    162 sg:person.016035276077.37 schema:affiliation https://www.grid.ac/institutes/grid.116068.8
    163 schema:familyName Meissner
    164 schema:givenName Alexander
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016035276077.37
    166 rdf:type schema:Person
    167 sg:pub.10.1038/nature04955 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014595021
    168 https://doi.org/10.1038/nature04955
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1038/nature05879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003888215
    171 https://doi.org/10.1038/nature05879
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    174 https://doi.org/10.1038/nature05934
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1038/nature05944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019109862
    177 https://doi.org/10.1038/nature05944
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1038/ng1973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039230968
    180 https://doi.org/10.1038/ng1973
    181 rdf:type schema:CreativeWork
    182 https://app.dimensions.ai/details/publication/pub.1078227860 schema:CreativeWork
    183 https://app.dimensions.ai/details/publication/pub.1082943978 schema:CreativeWork
    184 https://doi.org/10.1016/j.blre.2005.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022275744
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1016/j.molbrainres.2004.09.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016575240
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1016/j.stem.2007.05.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009469255
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1016/j.ydbio.2004.06.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021141005
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1016/s0960-9822(01)00459-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037472156
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1073/pnas.101118898 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016959164
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1111/j.1750-3639.2006.00010.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046892126
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1126/science.1116447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032194044
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1523/jneurosci.0428-04.200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024945874
    205 rdf:type schema:CreativeWork
    206 https://www.grid.ac/institutes/grid.116068.8 schema:alternateName Massachusetts Institute of Technology
    207 schema:name Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA.
    208 Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA.
    209 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...