A ROCK inhibitor permits survival of dissociated human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-06

AUTHORS

Kiichi Watanabe, Morio Ueno, Daisuke Kamiya, Ayaka Nishiyama, Michiru Matsumura, Takafumi Wataya, Jun B Takahashi, Satomi Nishikawa, Shin-ichi Nishikawa, Keiko Muguruma, Yoshiki Sasai

ABSTRACT

Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells. More... »

PAGES

681-686

Journal

TITLE

Nature Biotechnology

ISSUE

6

VOLUME

25

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1310

    DOI

    http://dx.doi.org/10.1038/nbt1310

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024578139

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17529971


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