A ROCK inhibitor permits survival of dissociated human embryonic stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-06

AUTHORS

Kiichi Watanabe, Morio Ueno, Daisuke Kamiya, Ayaka Nishiyama, Michiru Matsumura, Takafumi Wataya, Jun B Takahashi, Satomi Nishikawa, Shin-ichi Nishikawa, Keiko Muguruma, Yoshiki Sasai

ABSTRACT

Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells. More... »

PAGES

681-686

Journal

TITLE

Nature Biotechnology

ISSUE

6

VOLUME

25

Related Patents

  • Method For Culture Of Stem Cell
  • Induced Pluripotent Stem Cells Produced With Oct3/4, Klf4 And Sox2
  • Composition For Suspension Culturing Of Stem Cells
  • Mass Propagation Method For Hair Follicle Stem Cells
  • Method Of Subculturing Pluripotent Stem Cells
  • Kit For Making Induced Pluripotent Stem Cells Using Modified Rnas
  • Stem Cell Aggregate Suspension Compositions And Methods Of Differentiation Thereof
  • Methods For Production Of Platelets From Pluripotent Stem Cells And Compositions Thereof
  • Immortalization Of Epithelial Cells And Methods Of Use
  • Induced Pluripotent Stem Cells With Synthetic Modified Rnas
  • Stem Cell Culture Medium And Method
  • Reprogramming Cells
  • Cardiomyocyte Production
  • Method For Forming Endothelial Cells
  • Methods And Compositions For Cell Attachment And Cultivation On Planar Substrates
  • Composition For Reducing Cell Senescence Comprising Rho-Kinase Inhibitor And Use Thereof
  • Immortalization Of Epithelial Cells And Methods Of Use
  • Differentiation Of Human Embryonic Stem Cells
  • Composition For Suspension Culturing Of Stem Cells
  • Mass Propagation Method For Hair Follicle Stem Cells
  • Suspension And Clustering Of Human Pluripotent Stem Cells For Differentiation Into Pancreatic Endocrine Cells
  • Methods And Products For Transfecting Cells
  • Generation And Maintenance Of Stem Cells
  • Methods And Compositions For The Targeted Modification Of A Mouse Genome
  • Stem Cell Culture Medium And Method
  • Methods And Products For Expressing Proteins In Cells
  • Novel Method For Rapid And Efficient Generation Of Neuroectoderm Cells And Peripheral Neurons From Pluripotent Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Methods And Compositions For Stem Cell Cultures
  • Hla G-Modified Cells And Methods
  • Differentiation Of Pluripotent Stem Cells
  • Myogenic Differentiation Of Stem Cells And Uses Thereof
  • Cardiomyocyte Production
  • Media And Methods For Cell Culture
  • Clonal Culture Of Human Pluripotent Stem Cells
  • Substrates And Methods For Culturing Stem Cells
  • Use Of Fgf-2 And Rho Kinase Inhibitors In Methods Of Inducing Human Pluripotent Stem Cells
  • Compositions, Kits, And Methods For Making Induced Pluripotent Stem Cells Using Synthetic Modified Rnas
  • Differentiation Of Pluripotent Stem Cells To Definitive Endoderm Lineage
  • Use Of A Rock Inhibitor To Sustain Primary Human Keratinocytes In A Proliferative State
  • Technique For Isolating/Culturing Colorectal Epithelial Stem Cell, And Technique For Transplanting Colorectal Epithelium Employing Said Technique
  • 6-Substituted Isoquinolines And Isoquinolinones
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • A Method Of Preparing Isolated Cells And Uses
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Products For Expressing Proteins In Cells
  • Use Of Small Molecules To Enhance Mafa Expression In Pancreatic Endocrine Cells
  • Cardiomyocyte Production
  • Culture Of Pluripotent And Multipotent Cells On Microcarriers
  • Chemical Approaches For Generation Of Induced Pluripotent Stem Cells
  • Differentiation Of Pluripotent Stem Cells
  • Liver Organoid, Uses Thereof And Culture Method For Obtaining Them
  • Defined Media For Expansion And Maintenance Of Pluripotent Stem Cells
  • Kits Comprising Linear Dnas For Sustained Polypeptide Expression Using Synthetic, Modified Rnas
  • Chemical Reagents Regulating Stem Cell Fate
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising Said Stem Cells.
  • Chemically Defined Production Of Cardiomyocytes From Pluripotent Stem Cells
  • Human Pluripotent Stem Cells And Their Medical Use
  • Methods For Production Of Platelets From Pluripotent Stem Cells And Compositions Thereof
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Immortalization Of Epithelial Cells And Methods Of Use
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Method For Selecting Reduced Differentiation Resistance Human Induced Pluripotent Stem Cells
  • Differentiation Of Pluripotent Stem Cells
  • Generation And Maintenance Of Stem Cells
  • Cellular Therapy For Ocular Degeneration
  • Cardiomyocyte Production
  • Method For Producing Cerebellar Progenitor Tissue
  • Induction Of Pluripotent Cells
  • Methods And Products For Transfecting Cells
  • Methods And Products For Nucleic Acid Production And Delivery
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Innate Immune Suppression Enables Repeated Delivery Of Long Rna Molecules
  • Methods For Purifying Cells Derived From Pluripotent Stem Cells
  • Treatment Of Pluripotent Cells
  • Automated Method And Apparatus For Embryonic Stem Cell Culture
  • Scalable Primate Pluripotent Stem Cell Aggregate Suspension Culture And Differentiation Thereof
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Reprogramming Of Cells To A New Fate
  • Single Pluripotent Stem Cell Culture
  • Vitronectin-Derived Cell Culture Substrate And Uses Thereof
  • Method For Isolating And Culturing Adult Stem Cells Derived From Human Amniotic Epithelium
  • Cardiomyocyte Production
  • Method For Culture Of Stem Cell
  • Differentiation Of Human Embryonic Stem Cells
  • Seeding Cells On Porous Supports
  • Differentiation Of Human Pluripotent Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • In Vitro Differentiation Of Pluripotent Stem Cells To Pancreatic Endoderm Cells (Pec) And Endocrine Cells
  • Method Of Making Induced Pluripotent Stem Cells
  • 3-Dimensional Scaffolds For Improved Differentiation Of Pluripotent Stem Cells To Hepatocytes
  • Chemical Approaches For Generation Of Induced Pluripotent Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • 3-Dimensional Scaffolds For Improved Differentiation Of Pluripotent Stem Cells To Hepatocytes
  • Methods And Compositions For Stem Cell Cultures
  • Differentiation Of Human Embryonic Stem Cells
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising The Stem Cells
  • Method For Constructing Chimeric Rat Using Rat Embryonic Stem Cells
  • Method For Manufacturing Telencephalon Or Progenitor Tissue Thereof
  • Providing Ipscs To A Customer
  • Episomal Reprogramming With Chemicals
  • Nuclear Reprogramming Factor
  • Stem Cell Aggregate Suspension Compositions And Methods For Differentiation Thereof
  • Differentiation Of Human Embryonic Stem Cells Into Pancreatic Endocrine Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Pluripotent Stem Cell Culture On Micro-Carriers
  • Pluripotent Stem Cell-Derived 3d Retinal Tissue And Uses Thereof
  • Composition For Suspension Culturing Of Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Isolated Naive Pluripotent Stem Cells And Methods Of Generating Same
  • Titration Of Differentiation Medium Components
  • Culture Medium Containing Kinase Inhibitors, And Uses Thereof
  • Differentiation Of Human Embryonic Stem Cells
  • Chimeric Rat Produced Using Rat Embryonic Stem Cells In The Presence Of An Es Cell Differentiation Suppressant
  • Chemically Defined Production Of Cardiomyocytes From Pluripotent Stem Cells
  • Reprogramming Cells
  • Methods And Products For Expressing Proteins In Cells
  • Cardiomyocyte Production
  • Methods And Products For Transfecting Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Methods And Products For Expressing Proteins In Cells
  • Differentiation Of Pluripotent Stem Cells
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Peptide-Presenting Surfaces For Long-Term Culture Of Pluripotent Cells
  • Improved Culture Of Stem Cells
  • Oct3/4, Klf4, C-Myc And Sox2 Produce Induced Pluripotent Stem Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Immortalization Of Epithelial Cells And Methods Of Use
  • Differentiation Of Human Embryonic Stem Cells
  • Differentiation Of Human Pluripotent Stem Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Methods Of Neural Conversion Of Human Embryonic Stem Cells
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising Said Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Defined Media For Expansion And Maintenance Of Pluripotent Stem Cells
  • Differentiation Of Human Embryonic Stem Cells
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Method For Culture Of Stem Cell
  • Cardiomyocyte Production
  • Improved Culture Of Stem Cells
  • Chemically Defined Albumin-Free Conditions For Cardiomyocyte Differentiation Of Human Pluripotent Stem Cells
  • Stem Cell Aggregate Suspension Compositions And Methods For Differentiation Thereof
  • Method For Inducing Pluripotency In Human Somatic Cells With Prdm14 Or Nfrkb
  • Differentiation Of Human Embryonic Stem Cells
  • Xeno-Free And A Feeder Free Self-Renewal Extracellular Matrix For Long-Term Maintenance Of Undifferentiated Human Pluripotent Stem Cells And Method Of Synthesizing The Same
  • Generation Of Pluripotent Stem Cells Using Recombinant Proteins
  • Transplantation Of Neural Cells
  • Suspension And Clustering Of Human Pluripotent Stem Cells For Differentiation Into Pancreatic Endocrine Cells
  • Reprogramming Of Cells To A New Fate
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1310

    DOI

    http://dx.doi.org/10.1038/nbt1310

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024578139

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17529971


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Survival", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dose-Response Relationship, Drug", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryonic Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Intracellular Signaling Peptides and Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein-Serine-Threonine Kinases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pyridines", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "rho-Associated Kinases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "RIKEN Center for Developmental Biology", 
              "id": "https://www.grid.ac/institutes/grid.474692.a", 
              "name": [
                "Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Watanabe", 
            "givenName": "Kiichi", 
            "id": "sg:person.01045604551.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045604551.43"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Ueno", 
            "givenName": "Morio", 
            "id": "sg:person.01074315450.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074315450.48"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Kamiya", 
            "givenName": "Daisuke", 
            "id": "sg:person.01242251213.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242251213.75"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Nishiyama", 
            "givenName": "Ayaka", 
            "id": "sg:person.0620770651.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620770651.54"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Matsumura", 
            "givenName": "Michiru", 
            "id": "sg:person.01276261551.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276261551.18"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Wataya", 
            "givenName": "Takafumi", 
            "id": "sg:person.01207602217.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207602217.66"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Takahashi", 
            "givenName": "Jun B", 
            "id": "sg:person.014724716632.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014724716632.42"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Nishikawa", 
            "givenName": "Satomi", 
            "id": "sg:person.016660463762.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016660463762.08"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Nishikawa", 
            "givenName": "Shin-ichi", 
            "id": "sg:person.010611340305.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010611340305.82"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Muguruma", 
            "givenName": "Keiko", 
            "id": "sg:person.0667104051.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667104051.18"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Sasai", 
            "givenName": "Yoshiki", 
            "id": "sg:person.0726721457.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726721457.12"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature04960", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000099077", 
              "https://doi.org/10.1038/nature04960"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04960", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000099077", 
              "https://doi.org/10.1038/nature04960"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04960", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000099077", 
              "https://doi.org/10.1038/nature04960"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm1128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000604254", 
              "https://doi.org/10.1038/nrm1128"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm1128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000604254", 
              "https://doi.org/10.1038/nrm1128"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00439-005-1310-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004150659", 
              "https://doi.org/10.1007/s00439-005-1310-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00439-005-1310-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004150659", 
              "https://doi.org/10.1007/s00439-005-1310-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00439-005-1310-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004150659", 
              "https://doi.org/10.1007/s00439-005-1310-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0896-6273(00)00083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005164056"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0601990103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007572086"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1063", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008046577", 
              "https://doi.org/10.1038/nbt1063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1063", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008046577", 
              "https://doi.org/10.1038/nbt1063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1001-971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014515248", 
              "https://doi.org/10.1038/nbt1001-971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1001-971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014515248", 
              "https://doi.org/10.1038/nbt1001-971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.2005-0657", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015614145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(00)25449-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016676602"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1402", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019591995", 
              "https://doi.org/10.1038/nn1402"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1402", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019591995", 
              "https://doi.org/10.1038/nn1402"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nn1402", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019591995", 
              "https://doi.org/10.1038/nn1402"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021378655", 
              "https://doi.org/10.1038/nbt1189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021378655", 
              "https://doi.org/10.1038/nbt1189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0404700101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025625724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0600104103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026195174"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbrc.2006.04.135", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028081192"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0955-0674(00)00251-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030166395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0104-42", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033013561", 
              "https://doi.org/10.1038/nbt0104-42"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0104-42", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033013561", 
              "https://doi.org/10.1038/nbt0104-42"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/74447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033604670", 
              "https://doi.org/10.1038/74447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.00970", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034743567"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0896-6273(95)90262-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040412557"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.02723", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043244774"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(02)00835-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045218174"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1523/jneurosci.0295-04.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046350810"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0960-9822(01)00144-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047314499"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1634/stemcells.22-5-669", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047491648"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/76536", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051550210", 
              "https://doi.org/10.1038/76536"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/76536", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051550210", 
              "https://doi.org/10.1038/76536"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0079-6468(08)70068-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052167672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1517/14728222.9.4.715", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053625053"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1517/14728222.9.4.715", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053625053"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074628759", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2007-06", 
        "datePublishedReg": "2007-06-01", 
        "description": "Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt1310", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "25"
          }
        ], 
        "name": "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", 
        "pagination": "681-686", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "75170c16ca11b84e47b0ffeceb3c10eebb3e1b2e952c57291e38b704dec07c89"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "17529971"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt1310"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1024578139"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt1310", 
          "https://app.dimensions.ai/details/publication/pub.1024578139"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T23:11", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000424.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/nbt/journal/v25/n6/full/nbt1310.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1310'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1310'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1310'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1310'


     

    This table displays all metadata directly associated to this object as RDF triples.

    280 TRIPLES      21 PREDICATES      72 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt1310 schema:about N2026e4829c19492b88bc77ef8f190a59
    2 N2eaf1e6159324972a0a4cafc52227dc5
    3 N3d50b10734ed4b6d89f0a90039127436
    4 N4cda998147924c26a5fd18891db9d4fd
    5 N51a402c10cac42508cbbe7707c3620af
    6 N5c8522d200ff4093a07bfa022cbacf04
    7 N5dfa4906cfed4a11a5489aca3a684859
    8 N8a42c168d4814e8cb63770a7d167f982
    9 N976163e5b7b847d08a118bd7b56dda69
    10 Na0fb7f1a31b04252a1def06533bb0dd5
    11 Ncc76578e5ac843ccae46b2af4abb5995
    12 Nd8e04ee4b5594c90bc7e01537410f778
    13 Nf224b3cc1add4302a1f324e61ad25a9d
    14 anzsrc-for:06
    15 anzsrc-for:0601
    16 schema:author Nbf6e39e30aaf49e3834e19c84d2cf375
    17 schema:citation sg:pub.10.1007/s00439-005-1310-3
    18 sg:pub.10.1038/74447
    19 sg:pub.10.1038/76536
    20 sg:pub.10.1038/nature04960
    21 sg:pub.10.1038/nbt0104-42
    22 sg:pub.10.1038/nbt1001-971
    23 sg:pub.10.1038/nbt1063
    24 sg:pub.10.1038/nbt1189
    25 sg:pub.10.1038/nn1402
    26 sg:pub.10.1038/nrm1128
    27 https://app.dimensions.ai/details/publication/pub.1074628759
    28 https://doi.org/10.1006/dbio.2000.9912
    29 https://doi.org/10.1016/0896-6273(95)90262-7
    30 https://doi.org/10.1016/j.bbrc.2006.04.135
    31 https://doi.org/10.1016/s0076-6879(00)25449-9
    32 https://doi.org/10.1016/s0079-6468(08)70068-7
    33 https://doi.org/10.1016/s0092-8674(02)00835-8
    34 https://doi.org/10.1016/s0896-6273(00)00083-0
    35 https://doi.org/10.1016/s0955-0674(00)00251-9
    36 https://doi.org/10.1016/s0960-9822(01)00144-0
    37 https://doi.org/10.1073/pnas.0404700101
    38 https://doi.org/10.1073/pnas.0600104103
    39 https://doi.org/10.1073/pnas.0601990103
    40 https://doi.org/10.1126/science.282.5391.1145
    41 https://doi.org/10.1242/jcs.00970
    42 https://doi.org/10.1242/jcs.02723
    43 https://doi.org/10.1517/14728222.9.4.715
    44 https://doi.org/10.1523/jneurosci.0295-04.2004
    45 https://doi.org/10.1634/stemcells.2005-0657
    46 https://doi.org/10.1634/stemcells.22-5-669
    47 schema:datePublished 2007-06
    48 schema:datePublishedReg 2007-06-01
    49 schema:description Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells.
    50 schema:genre research_article
    51 schema:inLanguage en
    52 schema:isAccessibleForFree false
    53 schema:isPartOf N705c45b50f644d77b06cccd85031bf11
    54 Ncb0539a147844f79bc5cb16651c7e05a
    55 sg:journal.1115214
    56 schema:name A ROCK inhibitor permits survival of dissociated human embryonic stem cells
    57 schema:pagination 681-686
    58 schema:productId N20cc955a3ac6496facd7643aad9de203
    59 Nb9b88cab0a7344f8af329e56ea71150e
    60 Ndedd7fff0a59482cb1f0d0a403c1f3a5
    61 Nf3061e7fc982497c868c205c51194637
    62 Nf4931f3ed3194c0abd86dc07243bba6d
    63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024578139
    64 https://doi.org/10.1038/nbt1310
    65 schema:sdDatePublished 2019-04-10T23:11
    66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    67 schema:sdPublisher N87bcba6621a847be88be146cc9b80601
    68 schema:url http://www.nature.com/nbt/journal/v25/n6/full/nbt1310.html
    69 sgo:license sg:explorer/license/
    70 sgo:sdDataset articles
    71 rdf:type schema:ScholarlyArticle
    72 N05687f68a61d4063a1ca5e9ce2809fc8 rdf:first sg:person.01242251213.75
    73 rdf:rest N183c22a7015f45bc8ca95dc15baa6c63
    74 N16e4e02a9419457bb1fbfaf26b618e3a rdf:first sg:person.0726721457.12
    75 rdf:rest rdf:nil
    76 N183c22a7015f45bc8ca95dc15baa6c63 rdf:first sg:person.0620770651.54
    77 rdf:rest Na5919564c44a4c1ea4136fd6b9e6550e
    78 N2026e4829c19492b88bc77ef8f190a59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Cell Survival
    80 rdf:type schema:DefinedTerm
    81 N20cc955a3ac6496facd7643aad9de203 schema:name nlm_unique_id
    82 schema:value 9604648
    83 rdf:type schema:PropertyValue
    84 N2eaf1e6159324972a0a4cafc52227dc5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Amides
    86 rdf:type schema:DefinedTerm
    87 N3d50b10734ed4b6d89f0a90039127436 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Animals
    89 rdf:type schema:DefinedTerm
    90 N4cda998147924c26a5fd18891db9d4fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Embryonic Stem Cells
    92 rdf:type schema:DefinedTerm
    93 N51a402c10cac42508cbbe7707c3620af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Cell Differentiation
    95 rdf:type schema:DefinedTerm
    96 N5b0d313652d34604b46d31184d2e21dc rdf:first sg:person.0667104051.18
    97 rdf:rest N16e4e02a9419457bb1fbfaf26b618e3a
    98 N5c8522d200ff4093a07bfa022cbacf04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Pyridines
    100 rdf:type schema:DefinedTerm
    101 N5dfa4906cfed4a11a5489aca3a684859 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Intracellular Signaling Peptides and Proteins
    103 rdf:type schema:DefinedTerm
    104 N705c45b50f644d77b06cccd85031bf11 schema:issueNumber 6
    105 rdf:type schema:PublicationIssue
    106 N87bcba6621a847be88be146cc9b80601 schema:name Springer Nature - SN SciGraph project
    107 rdf:type schema:Organization
    108 N8a42c168d4814e8cb63770a7d167f982 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Mice
    110 rdf:type schema:DefinedTerm
    111 N976163e5b7b847d08a118bd7b56dda69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Cells, Cultured
    113 rdf:type schema:DefinedTerm
    114 Na0fb7f1a31b04252a1def06533bb0dd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Humans
    116 rdf:type schema:DefinedTerm
    117 Na5919564c44a4c1ea4136fd6b9e6550e rdf:first sg:person.01276261551.18
    118 rdf:rest Ne4e6062376594adf8324388f45ab624d
    119 Nb9b88cab0a7344f8af329e56ea71150e schema:name dimensions_id
    120 schema:value pub.1024578139
    121 rdf:type schema:PropertyValue
    122 Nbf6e39e30aaf49e3834e19c84d2cf375 rdf:first sg:person.01045604551.43
    123 rdf:rest Nd6e43ae178e74954bb6368efeb4236ac
    124 Ncb0539a147844f79bc5cb16651c7e05a schema:volumeNumber 25
    125 rdf:type schema:PublicationVolume
    126 Ncc76578e5ac843ccae46b2af4abb5995 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name rho-Associated Kinases
    128 rdf:type schema:DefinedTerm
    129 Nd6e43ae178e74954bb6368efeb4236ac rdf:first sg:person.01074315450.48
    130 rdf:rest N05687f68a61d4063a1ca5e9ce2809fc8
    131 Nd8e04ee4b5594c90bc7e01537410f778 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Protein-Serine-Threonine Kinases
    133 rdf:type schema:DefinedTerm
    134 Ndedd7fff0a59482cb1f0d0a403c1f3a5 schema:name pubmed_id
    135 schema:value 17529971
    136 rdf:type schema:PropertyValue
    137 Ne4e6062376594adf8324388f45ab624d rdf:first sg:person.01207602217.66
    138 rdf:rest Nec2d2a6e311443b5b3376f74915f496f
    139 Nec2d2a6e311443b5b3376f74915f496f rdf:first sg:person.014724716632.42
    140 rdf:rest Nfaf9f93867984a0cbfd76df56a774e17
    141 Nef042196263a48b79aaec70c3a10e5c7 rdf:first sg:person.010611340305.82
    142 rdf:rest N5b0d313652d34604b46d31184d2e21dc
    143 Nf224b3cc1add4302a1f324e61ad25a9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Dose-Response Relationship, Drug
    145 rdf:type schema:DefinedTerm
    146 Nf3061e7fc982497c868c205c51194637 schema:name readcube_id
    147 schema:value 75170c16ca11b84e47b0ffeceb3c10eebb3e1b2e952c57291e38b704dec07c89
    148 rdf:type schema:PropertyValue
    149 Nf4931f3ed3194c0abd86dc07243bba6d schema:name doi
    150 schema:value 10.1038/nbt1310
    151 rdf:type schema:PropertyValue
    152 Nfaf9f93867984a0cbfd76df56a774e17 rdf:first sg:person.016660463762.08
    153 rdf:rest Nef042196263a48b79aaec70c3a10e5c7
    154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Biological Sciences
    156 rdf:type schema:DefinedTerm
    157 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biochemistry and Cell Biology
    159 rdf:type schema:DefinedTerm
    160 sg:journal.1115214 schema:issn 1087-0156
    161 1546-1696
    162 schema:name Nature Biotechnology
    163 rdf:type schema:Periodical
    164 sg:person.01045604551.43 schema:affiliation https://www.grid.ac/institutes/grid.474692.a
    165 schema:familyName Watanabe
    166 schema:givenName Kiichi
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045604551.43
    168 rdf:type schema:Person
    169 sg:person.010611340305.82 schema:familyName Nishikawa
    170 schema:givenName Shin-ichi
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010611340305.82
    172 rdf:type schema:Person
    173 sg:person.01074315450.48 schema:familyName Ueno
    174 schema:givenName Morio
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074315450.48
    176 rdf:type schema:Person
    177 sg:person.01207602217.66 schema:familyName Wataya
    178 schema:givenName Takafumi
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207602217.66
    180 rdf:type schema:Person
    181 sg:person.01242251213.75 schema:familyName Kamiya
    182 schema:givenName Daisuke
    183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242251213.75
    184 rdf:type schema:Person
    185 sg:person.01276261551.18 schema:familyName Matsumura
    186 schema:givenName Michiru
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276261551.18
    188 rdf:type schema:Person
    189 sg:person.014724716632.42 schema:familyName Takahashi
    190 schema:givenName Jun B
    191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014724716632.42
    192 rdf:type schema:Person
    193 sg:person.016660463762.08 schema:familyName Nishikawa
    194 schema:givenName Satomi
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016660463762.08
    196 rdf:type schema:Person
    197 sg:person.0620770651.54 schema:familyName Nishiyama
    198 schema:givenName Ayaka
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620770651.54
    200 rdf:type schema:Person
    201 sg:person.0667104051.18 schema:familyName Muguruma
    202 schema:givenName Keiko
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667104051.18
    204 rdf:type schema:Person
    205 sg:person.0726721457.12 schema:familyName Sasai
    206 schema:givenName Yoshiki
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726721457.12
    208 rdf:type schema:Person
    209 sg:pub.10.1007/s00439-005-1310-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004150659
    210 https://doi.org/10.1007/s00439-005-1310-3
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1038/74447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033604670
    213 https://doi.org/10.1038/74447
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1038/76536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051550210
    216 https://doi.org/10.1038/76536
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1038/nature04960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000099077
    219 https://doi.org/10.1038/nature04960
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1038/nbt0104-42 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033013561
    222 https://doi.org/10.1038/nbt0104-42
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1038/nbt1001-971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014515248
    225 https://doi.org/10.1038/nbt1001-971
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1038/nbt1063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008046577
    228 https://doi.org/10.1038/nbt1063
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1038/nbt1189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021378655
    231 https://doi.org/10.1038/nbt1189
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1038/nn1402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019591995
    234 https://doi.org/10.1038/nn1402
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1038/nrm1128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000604254
    237 https://doi.org/10.1038/nrm1128
    238 rdf:type schema:CreativeWork
    239 https://app.dimensions.ai/details/publication/pub.1074628759 schema:CreativeWork
    240 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/0896-6273(95)90262-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040412557
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1016/j.bbrc.2006.04.135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028081192
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1016/s0076-6879(00)25449-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016676602
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1016/s0079-6468(08)70068-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052167672
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1016/s0092-8674(02)00835-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045218174
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1016/s0896-6273(00)00083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005164056
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/s0955-0674(00)00251-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030166395
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1016/s0960-9822(01)00144-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047314499
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1073/pnas.0404700101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025625724
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1073/pnas.0600104103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026195174
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1073/pnas.0601990103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007572086
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1242/jcs.00970 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034743567
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1242/jcs.02723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043244774
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1517/14728222.9.4.715 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053625053
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1523/jneurosci.0295-04.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046350810
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1634/stemcells.2005-0657 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015614145
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1634/stemcells.22-5-669 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047491648
    277 rdf:type schema:CreativeWork
    278 https://www.grid.ac/institutes/grid.474692.a schema:alternateName RIKEN Center for Developmental Biology
    279 schema:name Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan.
    280 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...