Fertile, Transgenic Oat Plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-12

AUTHORS

David A. Somers, Howard W. Rines, Weining Gu, Heidi F. Kaeppler, William R. Bushnell

ABSTRACT

Transgenic friable, embryogenic oat callus cultures were selected for phosphinothricin (PPT) resistance following microprojectile bombardment with a plasmid encoding the Streptomyces hygroscopicus bar gene and the Escherichia coli uidA gene. From three microprojectile bombardment experiments, 111 PPT-resistant tissue cultures were shown to be transgenic based on Southern blot analysis. From one to more than 20 copies of the transgenes were integrated in the genome of the PPT-resistant tissue cultures. Coexpression of β-glucuronidase (GUS) activity due to expression of uidA was detected in 75% of the transgenic tissue cultures. Plants were regenerated from 38 of the 111 transgenic tissue cultures. Regenerated plants generally exhibited male sterility; however, more than 30 plants regenerated from one transgenic tissue culture were fully fertile. GUS activity in the seed of fertile regenerated plants and PPT resistance in progeny of these plants cosegregated with bar and uidA sequences demonstrating stable inheritance of the transgenes. More... »

PAGES

1589

References to SciGraph publications

  • 1990-09. Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants in BIO/TECHNOLOGY
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1991-08. Anaerobic induction and tissue-specific expression of maize Adh1 promoter in transgenic rice plants and their progeny in MOLECULAR GENETICS AND GENOMICS
  • 1986-11. Efficient Transformation of Alfalfa Protoplasts by the Intranuclear Microinjection of Ti Plasmids in NATURE BIOTECHNOLOGY
  • 1992-08. Silicon carbide fiber-mediated stable transformation of plant cells in THEORETICAL AND APPLIED GENETICS
  • 1987-05. High-velocity microprojectiles for delivering nucleic acids into living cells in NATURE
  • 1991-08. Callus formation and plant regeneration from somatic embryos of oat (Avena sativa L.) in PLANT CELL REPORTS
  • 1987-12. Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids in THEORETICAL AND APPLIED GENETICS
  • 1990-05. Bialaphos selection of stable transformants from maize cell culture in THEORETICAL AND APPLIED GENETICS
  • 1987. Direct Gene Transfer to Plants in PLANT DNA INFECTIOUS AGENTS
  • 1991-10. Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos in NATURE BIOTECHNOLOGY
  • 1992-06. Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus in NATURE BIOTECHNOLOGY
  • 1992-01. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants in PLANT MOLECULAR BIOLOGY
  • 1985-11. Selection and regeneration of toxin-insensitive plants from tissue cultures of oats (Avena sativa) susceptible to Helminthosporium victoriae in THEORETICAL AND APPLIED GENETICS
  • 1992-03. Transformation of Microbes, Plants and Animals by Particle Bombardment in BIO/TECHNOLOGY
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    12

    VOLUME

    10

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt1292-1589

    DOI

    http://dx.doi.org/10.1038/nbt1292-1589

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004769388


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