Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-09-24

AUTHORS

Héctor García Martín, Natalia Ivanova, Victor Kunin, Falk Warnecke, Kerrie W Barry, Alice C McHardy, Christine Yeates, Shaomei He, Asaf A Salamov, Ernest Szeto, Eileen Dalin, Nik H Putnam, Harris J Shapiro, Jasmyn L Pangilinan, Isidore Rigoutsos, Nikos C Kyrpides, Linda Louise Blackall, Katherine D McMahon, Philip Hugenholtz

ABSTRACT

Enhanced biological phosphorus removal (EBPR) is one of the best-studied microbially mediated industrial processes because of its ecological and economic relevance. Despite this, it is not well understood at the metabolic level. Here we present a metagenomic analysis of two lab-scale EBPR sludges dominated by the uncultured bacterium, “Candidatus Accumulibacter phosphatis.” The analysis sheds light on several controversies in EBPR metabolic models and provides hypotheses explaining the dominance of A. phosphatis in this habitat, its lifestyle outside EBPR and probable cultivation requirements. Comparison of the same species from different EBPR sludges highlights recent evolutionary dynamics in the A. phosphatis genome that could be linked to mechanisms for environmental adaptation. In spite of an apparent lack of phylogenetic overlap in the flanking communities of the two sludges studied, common functional themes were found, at least one of them complementary to the inferred metabolism of the dominant organism. The present study provides a much needed blueprint for a systems-level understanding of EBPR and illustrates that metagenomics enables detailed, often novel, insights into even well-studied biological systems. More... »

PAGES

1263-1269

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt1247

DOI

http://dx.doi.org/10.1038/nbt1247

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009864240

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16998472


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25 EBPR metabolic models
26 EBPR sludge
27 adaptation
28 analysis
29 apparent lack
30 bacterium
31 biological phosphorus removal
32 biological systems
33 blueprint
34 common functional theme
35 community
36 comparison
37 controversy
38 cultivation requirements
39 dominance
40 dominant organisms
41 dynamics
42 economic relevance
43 environmental adaptation
44 evolutionary dynamics
45 functional themes
46 genome
47 habitats
48 hypothesis
49 industrial processes
50 inferred metabolism
51 insights
52 lack
53 levels
54 lifestyle
55 light
56 mechanism
57 metabolic level
58 metabolic models
59 metabolism
60 metagenomic analysis
61 metagenomics
62 model
63 organisms
64 overlap
65 phosphorus removal
66 phylogenetic overlap
67 present study
68 process
69 relevance
70 removal
71 requirements
72 same species
73 sludge
74 sludge communities
75 species
76 spite
77 study
78 system
79 systems-level understanding
80 themes
81 uncultured bacterium
82 understanding
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