Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Brian F Pfleger, Douglas J Pitera, Christina D Smolke, Jay D Keasling

ABSTRACT

Many applications of synthetic biology require the balanced expression of multiple genes. Although operons facilitate coordinated expression of multiple genes in prokaryotes and eukaryotes, coordinating the many post-transcriptional processes that determine the relative levels of gene expression in operons by a priori design remains a challenge. We describe a method for tuning the expression of multiple genes within operons by generating libraries of tunable intergenic regions (TIGRs), recombining various post-transcriptional control elements and screening for the desired relative expression levels. TIGRs can vary the relative expression of two reporter genes over a 100-fold range and balance expression of three genes in an operon that encodes a heterologous mevalonate biosynthetic pathway, resulting in a sevenfold increase in mevalonate production. This technology should be useful for optimizing the expression of multiple genes in synthetic operons, both in prokaryotes and eukaryotes. More... »

PAGES

1027-1032

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nbt1226

DOI

http://dx.doi.org/10.1038/nbt1226

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026689119

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16845378


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Algorithms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Intergenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Operon", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, Berkeley", 
          "id": "https://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Chemical Engineering, University of California-Berkeley, Berkeley, CA 94720-1462, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pfleger", 
        "givenName": "Brian F", 
        "id": "sg:person.01325623305.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325623305.34"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Pitera", 
        "givenName": "Douglas J", 
        "id": "sg:person.0746745321.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746745321.14"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Smolke", 
        "givenName": "Christina D", 
        "id": "sg:person.0637537051.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637537051.10"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Keasling", 
        "givenName": "Jay D", 
        "id": "sg:person.01260740563.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260740563.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1101/gad.1348805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000688422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m303495200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001519746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.10434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004194801"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.66.12.5399-5405.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004438660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15216540400022441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004911465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd1256", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006425891", 
          "https://doi.org/10.1038/nrd1256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrd1256", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006425891", 
          "https://doi.org/10.1038/nrd1256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.micro.58.030603.123841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010774457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkg595", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013618001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.20630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013767826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.20630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013767826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(00)24237-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017231365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.105.020727", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021079457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt833", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022297654", 
          "https://doi.org/10.1038/nbt833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt833", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022297654", 
          "https://doi.org/10.1038/nbt833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkg690", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022643124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1472-6750-6-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025295777", 
          "https://doi.org/10.1186/1472-6750-6-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10409230590918702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025759589"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025786828", 
          "https://doi.org/10.1038/nature04342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025786828", 
          "https://doi.org/10.1038/nature04342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025786828", 
          "https://doi.org/10.1038/nature04342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.2153206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028595192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.9.2422-2427.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033369697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2003.03545.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039257699"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040856976", 
          "https://doi.org/10.1038/nbt986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040856976", 
          "https://doi.org/10.1038/nbt986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041144702", 
          "https://doi.org/10.1038/nrm1403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041144702", 
          "https://doi.org/10.1038/nrm1403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041144702", 
          "https://doi.org/10.1038/nrm1403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1994.1714", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043561676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2443.2002.00563.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047790611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1996.6121101.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048548919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1996.6121101.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048548919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.r400041200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050753081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13657", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052329354", 
          "https://doi.org/10.1038/13657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/13657", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052329354", 
          "https://doi.org/10.1038/13657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90508-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052591605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075344119", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-08", 
    "datePublishedReg": "2006-08-01", 
    "description": "Many applications of synthetic biology require the balanced expression of multiple genes. Although operons facilitate coordinated expression of multiple genes in prokaryotes and eukaryotes, coordinating the many post-transcriptional processes that determine the relative levels of gene expression in operons by a priori design remains a challenge. We describe a method for tuning the expression of multiple genes within operons by generating libraries of tunable intergenic regions (TIGRs), recombining various post-transcriptional control elements and screening for the desired relative expression levels. TIGRs can vary the relative expression of two reporter genes over a 100-fold range and balance expression of three genes in an operon that encodes a heterologous mevalonate biosynthetic pathway, resulting in a sevenfold increase in mevalonate production. This technology should be useful for optimizing the expression of multiple genes in synthetic operons, both in prokaryotes and eukaryotes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nbt1226", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1115214", 
        "issn": [
          "1087-0156", 
          "1546-1696"
        ], 
        "name": "Nature Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "24"
      }
    ], 
    "name": "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", 
    "pagination": "1027-1032", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7f24f19ab018a5953b95d6254f37d8dfc6a28a8571efe1aba04c1c2b6a47bd66"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16845378"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9604648"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nbt1226"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026689119"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nbt1226", 
      "https://app.dimensions.ai/details/publication/pub.1026689119"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53990_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nbt/journal/v24/n8/full/nbt1226.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt1226'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt1226'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt1226'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt1226'


 

This table displays all metadata directly associated to this object as RDF triples.

209 TRIPLES      21 PREDICATES      65 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nbt1226 schema:about N16aae87ab52b4313bc918b37ffa728a5
2 N64c5d40d07554c1385145ac63831857f
3 N77351d896ada44c2a5981c78d1500951
4 N837d1afc39ad4b12a2fdd3c62c7c02d0
5 N8a16b2908033430cb3c849c5ca4d90d4
6 N8d3e7a922abc4834a5861fcad506bffe
7 Nb94e0698b9384f26a328db910333b8c2
8 Nf2231953b01748b0bc1b5fcc7bc0d577
9 anzsrc-for:06
10 anzsrc-for:0604
11 schema:author N61b8ebd8e2a043bebe5b530b4342ba59
12 schema:citation sg:pub.10.1038/13657
13 sg:pub.10.1038/nature04342
14 sg:pub.10.1038/nbt833
15 sg:pub.10.1038/nbt986
16 sg:pub.10.1038/nrd1256
17 sg:pub.10.1038/nrm1403
18 sg:pub.10.1186/1472-6750-6-4
19 https://app.dimensions.ai/details/publication/pub.1075344119
20 https://doi.org/10.1002/bit.10434
21 https://doi.org/10.1002/bit.20630
22 https://doi.org/10.1006/jmbi.1994.1714
23 https://doi.org/10.1016/0092-8674(88)90508-9
24 https://doi.org/10.1016/s0076-6879(00)24237-7
25 https://doi.org/10.1046/j.1365-2443.2002.00563.x
26 https://doi.org/10.1046/j.1365-2958.1996.6121101.x
27 https://doi.org/10.1046/j.1365-2958.2003.03545.x
28 https://doi.org/10.1074/jbc.m303495200
29 https://doi.org/10.1074/jbc.r400041200
30 https://doi.org/10.1080/10409230590918702
31 https://doi.org/10.1080/15216540400022441
32 https://doi.org/10.1093/nar/gkg595
33 https://doi.org/10.1093/nar/gkg690
34 https://doi.org/10.1101/gad.1348805
35 https://doi.org/10.1124/mol.105.020727
36 https://doi.org/10.1128/aem.66.12.5399-5405.2000
37 https://doi.org/10.1128/jb.182.9.2422-2427.2000
38 https://doi.org/10.1146/annurev.micro.58.030603.123841
39 https://doi.org/10.1261/rna.2153206
40 schema:datePublished 2006-08
41 schema:datePublishedReg 2006-08-01
42 schema:description Many applications of synthetic biology require the balanced expression of multiple genes. Although operons facilitate coordinated expression of multiple genes in prokaryotes and eukaryotes, coordinating the many post-transcriptional processes that determine the relative levels of gene expression in operons by a priori design remains a challenge. We describe a method for tuning the expression of multiple genes within operons by generating libraries of tunable intergenic regions (TIGRs), recombining various post-transcriptional control elements and screening for the desired relative expression levels. TIGRs can vary the relative expression of two reporter genes over a 100-fold range and balance expression of three genes in an operon that encodes a heterologous mevalonate biosynthetic pathway, resulting in a sevenfold increase in mevalonate production. This technology should be useful for optimizing the expression of multiple genes in synthetic operons, both in prokaryotes and eukaryotes.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree false
46 schema:isPartOf N0620b214aa8044048ef0e24a135f8214
47 N6cc2ee02590c4e4292431e57e365b53d
48 sg:journal.1115214
49 schema:name Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
50 schema:pagination 1027-1032
51 schema:productId N3110e9905c7a4b74bf58e1ccdde0343b
52 N56e0b8ec2fbf4b1c894ffef73b4f38a5
53 N5952416399bf48babeceddac5b1b606c
54 N9d97f4ba16694a3e84f01f8717ee5d89
55 Nad997da6f1064046a876fc62ac0c71e0
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026689119
57 https://doi.org/10.1038/nbt1226
58 schema:sdDatePublished 2019-04-11T12:12
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher N8db1d7c82afc431c8b45b08a54122317
61 schema:url http://www.nature.com/nbt/journal/v24/n8/full/nbt1226.html
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N0620b214aa8044048ef0e24a135f8214 schema:issueNumber 8
66 rdf:type schema:PublicationIssue
67 N16aae87ab52b4313bc918b37ffa728a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Algorithms
69 rdf:type schema:DefinedTerm
70 N3110e9905c7a4b74bf58e1ccdde0343b schema:name doi
71 schema:value 10.1038/nbt1226
72 rdf:type schema:PropertyValue
73 N53d9ce956e304d9e9470a21b8d06d19c rdf:first sg:person.01260740563.80
74 rdf:rest rdf:nil
75 N56e0b8ec2fbf4b1c894ffef73b4f38a5 schema:name dimensions_id
76 schema:value pub.1026689119
77 rdf:type schema:PropertyValue
78 N5952416399bf48babeceddac5b1b606c schema:name nlm_unique_id
79 schema:value 9604648
80 rdf:type schema:PropertyValue
81 N61b8ebd8e2a043bebe5b530b4342ba59 rdf:first sg:person.01325623305.34
82 rdf:rest N9b616b9838d44f40bdffa6c88d4c2c30
83 N64c5d40d07554c1385145ac63831857f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Gene Expression
85 rdf:type schema:DefinedTerm
86 N6cc2ee02590c4e4292431e57e365b53d schema:volumeNumber 24
87 rdf:type schema:PublicationVolume
88 N77351d896ada44c2a5981c78d1500951 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Gene Library
90 rdf:type schema:DefinedTerm
91 N821595708a95491e9fd87800327138a0 rdf:first sg:person.0637537051.10
92 rdf:rest N53d9ce956e304d9e9470a21b8d06d19c
93 N837d1afc39ad4b12a2fdd3c62c7c02d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Gene Transfer Techniques
95 rdf:type schema:DefinedTerm
96 N8a16b2908033430cb3c849c5ca4d90d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Operon
98 rdf:type schema:DefinedTerm
99 N8d3e7a922abc4834a5861fcad506bffe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Genetic Engineering
101 rdf:type schema:DefinedTerm
102 N8db1d7c82afc431c8b45b08a54122317 schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 N9b616b9838d44f40bdffa6c88d4c2c30 rdf:first sg:person.0746745321.14
105 rdf:rest N821595708a95491e9fd87800327138a0
106 N9d97f4ba16694a3e84f01f8717ee5d89 schema:name readcube_id
107 schema:value 7f24f19ab018a5953b95d6254f37d8dfc6a28a8571efe1aba04c1c2b6a47bd66
108 rdf:type schema:PropertyValue
109 Nad997da6f1064046a876fc62ac0c71e0 schema:name pubmed_id
110 schema:value 16845378
111 rdf:type schema:PropertyValue
112 Nb94e0698b9384f26a328db910333b8c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name DNA, Intergenic
114 rdf:type schema:DefinedTerm
115 Nf2231953b01748b0bc1b5fcc7bc0d577 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Gene Expression Regulation
117 rdf:type schema:DefinedTerm
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
122 schema:name Genetics
123 rdf:type schema:DefinedTerm
124 sg:journal.1115214 schema:issn 1087-0156
125 1546-1696
126 schema:name Nature Biotechnology
127 rdf:type schema:Periodical
128 sg:person.01260740563.80 schema:familyName Keasling
129 schema:givenName Jay D
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260740563.80
131 rdf:type schema:Person
132 sg:person.01325623305.34 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
133 schema:familyName Pfleger
134 schema:givenName Brian F
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325623305.34
136 rdf:type schema:Person
137 sg:person.0637537051.10 schema:familyName Smolke
138 schema:givenName Christina D
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637537051.10
140 rdf:type schema:Person
141 sg:person.0746745321.14 schema:familyName Pitera
142 schema:givenName Douglas J
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746745321.14
144 rdf:type schema:Person
145 sg:pub.10.1038/13657 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052329354
146 https://doi.org/10.1038/13657
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/nature04342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025786828
149 https://doi.org/10.1038/nature04342
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/nbt833 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022297654
152 https://doi.org/10.1038/nbt833
153 rdf:type schema:CreativeWork
154 sg:pub.10.1038/nbt986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040856976
155 https://doi.org/10.1038/nbt986
156 rdf:type schema:CreativeWork
157 sg:pub.10.1038/nrd1256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006425891
158 https://doi.org/10.1038/nrd1256
159 rdf:type schema:CreativeWork
160 sg:pub.10.1038/nrm1403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041144702
161 https://doi.org/10.1038/nrm1403
162 rdf:type schema:CreativeWork
163 sg:pub.10.1186/1472-6750-6-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025295777
164 https://doi.org/10.1186/1472-6750-6-4
165 rdf:type schema:CreativeWork
166 https://app.dimensions.ai/details/publication/pub.1075344119 schema:CreativeWork
167 https://doi.org/10.1002/bit.10434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004194801
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1002/bit.20630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013767826
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1006/jmbi.1994.1714 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043561676
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/0092-8674(88)90508-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052591605
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/s0076-6879(00)24237-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017231365
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1046/j.1365-2443.2002.00563.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047790611
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1046/j.1365-2958.1996.6121101.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048548919
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1046/j.1365-2958.2003.03545.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039257699
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1074/jbc.m303495200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001519746
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1074/jbc.r400041200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050753081
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1080/10409230590918702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025759589
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1080/15216540400022441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004911465
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1093/nar/gkg595 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013618001
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1093/nar/gkg690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022643124
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1101/gad.1348805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000688422
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1124/mol.105.020727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021079457
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1128/aem.66.12.5399-5405.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004438660
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1128/jb.182.9.2422-2427.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033369697
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1146/annurev.micro.58.030603.123841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010774457
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1261/rna.2153206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028595192
206 rdf:type schema:CreativeWork
207 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
208 schema:name Department of Chemical Engineering, University of California-Berkeley, Berkeley, CA 94720-1462, USA.
209 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...